Regulation of plant hormone receptor using stereochemical isomers of a natural product

• Project/Area Number: 18KK0162
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 37:Biomolecular chemistry and related fields
• Research Institution: Tohoku University
• Principal Investigator: UEDA Minoru 東北大学, 理学研究科, 教授 (60265931)
• Co-Investigator(Kenkyū-buntansha): 岡田 憲典 東京大学, 生物生産工学研究センター(現在使用不可), 准教授 (20312241) ; 高岡 洋輔 東北大学, 理学研究科, 准教授 (80599762) ; 宮本 皓司 帝京大学, 理工学部, 講師 (90721514) ; 加治 拓哉 東北大学, 理学研究科, 助教 (80835520)
• Project Period (FY): 2019-02-07 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2020: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2019: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000); Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
• Keywords: 植物ホルモン / ジャスモン酸 / 受容体 / 遺伝的冗長性 / コロナチン / 立体異性体 / 化学プローブ / 標的選択性 / 活性チューニング / 二次代謝

Outline of Final Research Achievements

A stereoisomer library of phytotoxins was constructed by chemical synthesis, and a comprehensive evaluation of their affinity for phytohormone receptor subtypes and their biological activity was conducted in an international collaboration with CNB-CSIC, Spain. The stereoisomer library was evaluated using three plant species, Arabidopsis thaliana, tomato, and rice, and we succeeded in finding stereoisomers each showing unique selective affinity to nuclear receptor subtypes.
In this study, we focused on stereoisomers that showed affinity to the receptor subtypes in Arabidopsis thaliana and analyzed their mechanisms of action in detail. As a result, we succeeded in developing molecules that selectively activate receptor subtypes of phytohormone receptors that are involved in the induction of plant resistance to microbial infection.The molecule selectively activates only a portion of phytohormone signaling, while suppression of gene expression does not occur to any degree.

Academic Significance and Societal Importance of the Research Achievements

通常では、植物の病原菌感染耐性は、植物の生長阻害とトレードオフの関係にあるが、本手法ではこれらを切り離して活性化できることが明らかになり、既存の遺伝学的手法と比較して、ケミカルバイオロジー手法の優れた点を示すことができた。本分子は植物ホルモンのシグナル伝達の内の一部のみを選択的に活性化する一方で、遺伝子発現の抑制は歩と度起こさないことが明らかになった。これによって、植物の生長防御には、細胞壁の生合成を抑制する遺伝子群の発現が関与することが明らかになった。

Research Products

• [Int'l Joint Research] CNB-CSIC(スペイン)
• [Int'l Joint Research] スペイン国立生物工学研究所(スペイン)
• [Int'l Joint Research] マドリード自治大学(スペイン)
• [Journal Article] Protein?protein interactions between jasmonate-related master regulator MYC and transcriptional mediator MED25 depend on a short binding domain (2022)
  • Author(s): Takaoka Yousuke、Suzuki Kaho、Nozawa Akira、Takahashi Hirotaka、Sawasaki Tatsuya、Ueda Minoru
  • Journal Title: Journal of Biological Chemistry Volume: 298 Issue: 1 Pages: 101504-101504
  • DOI: 10.1016/j.jbc.2021.101504
  • Peer Reviewed / Open Access
• [Journal Article] Extended JAZ degron sequence for plant hormone binding in jasmonate co-receptor of tomato SlCOI1-SlJAZ (2021)
  • Author(s): Saito Rina、Hayashi Kengo、Nomoto Haruna、Nakayama Misuzu、Takaoka Yousuke、Saito Hiroaki、Yamagami Souhei、Muto Toshiya、Ueda Minoru
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 13612-13612
  • DOI: 10.1038/s41598-021-93067-1
  • Peer Reviewed / Open Access
• [Journal Article] Syntheses of dinor-cis/iso-12-oxo-phytodienoic acid (dn-cis/iso-OPDAs), ancestral jasmonate phytohormones of the bryophyte Marchantia polymorpha L., and their catabolites (2021)
  • Author(s): Wang Jianxin、Sakurai Haruka、Kato Nobuki、Kaji Takuya、Ueda Minoru
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 2033-2033
  • DOI: 10.1038/s41598-021-81575-z
  • Peer Reviewed / Open Access
• [Journal Article] Rational design of a stapled JAZ9 peptide inhibiting protein?protein interaction of a plant transcription factor (2021)
  • Author(s): Suzuki Kaho、Takaoka Yousuke、Ueda Minoru
  • Journal Title: RSC Chemical Biology Volume: 2 Issue: 2 Pages: 499-502
  • DOI: 10.1039/d0cb00204f
  • Peer Reviewed / Open Access
• [Journal Article] Deciphering OPDA signaling components in the momilactone-producing moss Calohypnum plumiforme (2021)
  • Author(s): Hideo Inagaki, Koji Miyamoto, Noriko Ando, Kohei Murakami, Koki Sugisawa, Shion Morita, Emi Yumoto, Miyu Teruya, Kenichi Uchida, Nobuki Kato, Takuya Kaji, Yousuke Takaoka, Yuko Hojo, Tomonori Shinya, Ivan Galis, Akira Nozawa, Tatsuya Sawasaki, Hideaki Nojiri, Minoru Ueda, Kazunori Okada
  • Journal Title: Frontiers in Plant Science Volume: - Pages: 688565-688565
  • DOI: 10.3389/fpls.2021.688565
  • Peer Reviewed / Open Access
• [Journal Article] Hoechst-tagged Fluorescein Diacetate for Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana (2020)
  • Author(s): Y. Takaoka, S. Miyagawa, A. Nakamura, S. Egoshi, S. Tsukiji, M. Ueda
  • Journal Title: Sci. Rep. Volume: 10 Issue: 1 Pages: 5333-5333
  • DOI: 10.1038/s41598-020-62239-w
  • Peer Reviewed / Open Access
• [Journal Article] Scalable synthesis of (+)-coronafacic acid, (2020)
  • Author(s): N. Kato, S. Miyagawa, H. Nomoto, M. Nakayama, M. Iwashita, M. Ueda
  • Journal Title: Chirality Volume: 32 Issue: 4 Pages: 423-430
  • DOI: 10.1002/chir.23172
  • Peer Reviewed
• [Journal Article] Stereoselective Syntheses of all the Possible Stereoisomers of Coronafacic Acid (2020)
  • Author(s): Watanabe Raku、Kato Nobuki、Hayashi Kengo、Tozawa Sho、Ogura Yusuke、Kuwahara Shigefumi、Ueda Minoru
  • Journal Title: ChemistryOpen Volume: 9 Issue: 10 Pages: 1008-1017
  • DOI: 10.1002/open.202000210
  • Peer Reviewed