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Extensive characterization of domestication-related genes in buckwheat by utilizing the genetic resource of Yunnan province, China

Research Project

Project/Area Number 18KK0172
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionKyoto University

Principal Investigator

Yasui Yasuo  京都大学, 農学研究科, 助教 (70293917)

Co-Investigator(Kenkyū-buntansha) 水野 信之  京都大学, 農学研究科, 特定研究員 (30829180)
FAWCETT JEFFREY  国立研究開発法人理化学研究所, 数理創造プログラム, 上級研究員 (50727394)
大迫 敬義  京都府立大学, 生命環境科学研究科, 准教授 (80363969)
Project Period (FY) 2018-10-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2020: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2019: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywords栽培植物起原 / ソバ / 変異導入技術 / ゲノム / 遺伝資源 / 孤児作物 / 栽培植物起原学 / 栽培ソバ / 野生ソバ / ゲノムスキャン / 中国 / 雲南省 / 四川省 / チベット自治区 / 東チベット / ソバの起原 / 栽培化 / 栽培化関連遺伝子 / NGS
Outline of Final Research Achievements

In this study, we aimed to identify genes related to the domestication of buckwheat by conducting the following steps: 1) We collected germplasm from both wild and cultivated buckwheat in Yunnan Province, China, and sequenced their genomes; 2) We identified genomic regions that exhibited significant differences in genetic diversity between the wild and cultivated buckwheat populations, which we classified as artificially selected regions. We confirmed that the ancestral wild population from southeastern Tibet is the closest relative to the cultivated species. Notably, a signature of artificial selection was discovered within the 52-53 Mb interval on the 1st chromosome. This region contains a specific genotype that was selected from the ancestral wild population in southeastern Tibet and has now proliferated among cultivated buckwheat varieties globally. Additionally, we successfully applied the NGS-TILLING technique to buckwheat, establishing a robust system for introducing mutations.

Academic Significance and Societal Importance of the Research Achievements

主要作物と比較して、地域的・伝統的な重要性を持つ多くの作物では、商業価値が低いために育種学的な研究が進んでいない。これらの作物は孤児作物と呼ばれている。本研究では中国と英国の研究者との連携のもと、孤児作物の1つであるソバのゲノムを解読し、その栽培化に重要なゲノム領域の同定に成功した。また、組換え技術やゲノム編集技術を使わずに遺伝子を改変することにも成功した。今回の成果は、発展途上国を含めた世界中での孤児作物の育種に貢献する。さらに、各地域でよく根付いている孤児作物の改良は、農家の収入向上をもたらすとともに、食料問題の解決にも貢献する。(本成果は、Nature Plants誌に掲載された。)

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (35 results)

All 2023 2022 2021 2020 2019 2018 Other

All Int'l Joint Research (4 results) Journal Article (16 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 14 results,  Open Access: 12 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results)

  • [Int'l Joint Research] 雲南農業大学(中国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ケンブリッジ大学(英国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Yunnan Agricultural University(中国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] University of Cambridge(英国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Development and chromosomal characterization of interspecific hybrids between common buckwheat (<i>Fagopyrum esculentum</i>) and a related perennial species (<i>F. cymosum</i>)2023

    • Author(s)
      Sugiyama Mari、Norizuki Miyu、Kikuchi Shinji、Yasui Yasuo、Matsui Katsuhiro
    • Journal Title

      Breeding Science

      Volume: 73 Issue: 2 Pages: 230-236

    • DOI

      10.1270/jsbbs.22063

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome sequencing reveals the genetic architecture of heterostyly and domestication history of common buckwheat2023

    • Author(s)
      Fawcett JA, Takeshima R, Kikuchi S, Yazaki E, Katsube-Tanaka T, Dong Y, Li M, Hunt HV, Jones MK, Lister DL, Ohsako T, Ogiso-Tanaka E, Fujii K, Hara T, Matsui K, Mizuno N, Nishimura K, Nakazaki T, Saito H, Takeuchi N, Ueno M, Matsumoto D, Norizuki M, Shirasawa K, Li C, Hirakawa H, Ota T, Yasui Y.
    • Journal Title

      Nature Plants

      Volume: 9 Issue: 8 Pages: 1236-1251

    • DOI

      10.1038/s41477-023-01474-1

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] New locality and bud growth of the world biggest flower, <i>Rafflesia tuan-mudae,</i> in Naha Jaley, Sarawak, Malaysia2022

    • Author(s)
      Diway Bibian、Yasui Yasuo、Innan Hideki、Takeuchi Yayoi
    • Journal Title

      Tropics

      Volume: 30 Issue: 4 Pages: 71-82

    • DOI

      10.3759/tropics.MS21-14

    • NAID

      130008165603

    • ISSN
      0917-415X, 1882-5729
    • Year and Date
      2022-03-01
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chromosome-scale genome assembly of a Japanese chili pepper landrace, <i>Capsicum annuum</i> ‘Takanotsume’2022

    • Author(s)
      Shirasawa Kenta、Hosokawa Munetaka、Yasui Yasuo、Toyoda Atsushi、Isobe Sachiko
    • Journal Title

      DNA Research

      Volume: 30 Issue: 1

    • DOI

      10.1093/dnares/dsac052

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A <i>CLAVATA3</i>-like Gene Acts as a Gynoecium Suppression Function in White Campion2022

    • Author(s)
      Kazama Yusuke、Kitoh Moe、Kobayashi Taiki、Ishii Kotaro、Krasovec Marc、Yasui Yasuo、Abe Tomoko、Kawano Shigeyuki、Filatov Dmitry A
    • Journal Title

      Molecular Biology and Evolution

      Volume: 39 Issue: 10

    • DOI

      10.1093/molbev/msac195

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Degenerate oligonucleotide primer MIG-seq: an effective PCR-based method for high-throughput genotyping2022

    • Author(s)
      Nishimura Kazusa、Kokaji Hiroyuki、Motoki Ko、Yamazaki Akira、Nagasaka Kyoka、Takisawa Rihito、Yasui Yasuo、Kawai Takashi、Ushijima Koichiro、Yamasaki Masanori、Saito Hiroki、Nakano Ryohei、Nakazaki Tetsuya
    • Journal Title

      bioRxiv

      Volume: 2022.08.25 Pages: 504752-504752

    • DOI

      10.1101/2022.08.25.504752

    • Related Report
      2022 Research-status Report
    • Open Access
  • [Journal Article] MIG-seq is an effective method for high-throughput genotyping in wheat (<i>Triticum</i> spp.)2022

    • Author(s)
      Nishimura Kazusa、Motoki Ko、Yamazaki Akira、Takisawa Rihito、Yasui Yasuo、Kawai Takashi、Ushijima Koichiro、Nakano Ryohei、Nakazaki Tetsuya
    • Journal Title

      DNA Research

      Volume: 29 Issue: 2

    • DOI

      10.1093/dnares/dsac011

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 野生ソバの遺伝資源を求めて起原地へ, そしてゲノム育種へ2022

    • Author(s)
      安井 康夫, ジェフリ フォーセット, 大迫 敬義
    • Journal Title

      アグリバイオ

      Volume: 6 Pages: 23-27

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] 野生ソバの遺伝資源を求めて起原地へ、そしてゲノム育種へ2022

    • Author(s)
      安井康夫、ジェフリー フォーセット、大迫敬義
    • Journal Title

      アグリバイオ

      Volume: 6 Pages: 23-27

    • Related Report
      2021 Research-status Report
  • [Journal Article] Targeted amplicon sequencing + next-generation sequencing?based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)2021

    • Author(s)
      Takeshima Ryoma、Ogiso-Tanaka Eri、Yasui Yasuo、Matsui Katsuhiro
    • Journal Title

      BMC Plant Biology

      Volume: 21 Issue: 1 Pages: 18-18

    • DOI

      10.1186/s12870-020-02790-w

    • Related Report
      2021 Research-status Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The genotype-dependent phenotypic landscape of quinoa in salt tolerance and key growth traits2020

    • Author(s)
      Mizuno Nobuyuki、Toyoshima Masami、Fujita Miki、Fukuda Shota、Kobayashi Yasufumi、Ueno Mariko、Tanaka Kojiro、Tanaka Tsutomu、Nishihara Eiji、Mizukoshi Hiroharu、Yasui Yasuo、Fujita Yasunari
    • Journal Title

      DNA Research

      Volume: 27 Issue: 4

    • DOI

      10.1093/dnares/dsaa022

    • NAID

      120006892056

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genetic and genomic research for the development of an efficient breeding system in heterostylous self-incompatible common buckwheat (Fagopyrum esculentum)2020

    • Author(s)
      Matsui Katsuhiro、Yasui Yasuo
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 133 Issue: 5 Pages: 1641-1653

    • DOI

      10.1007/s00122-020-03572-6

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] History of the progressive development of genetic marker systems for common buckwheat2020

    • Author(s)
      Yasuo Yasui
    • Journal Title

      Breeding Science

      Volume: 70 Issue: 1 Pages: 13-18

    • DOI

      10.1270/jsbbs.19075

    • NAID

      130007809718

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Buckwheat heteromorphic self-incompatibility: genetics, genomics and application to breeding2020

    • Author(s)
      Katsuhiro Matsui, Yasuo Yasui
    • Journal Title

      Breeding Science

      Volume: 70 Issue: 1 Pages: 32-38

    • DOI

      10.1270/jsbbs.19083

    • NAID

      130007809720

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Classification and systematics of the <i>Fagopyrum</i> species2020

    • Author(s)
      Takanori Ohsako, Chengyun Li
    • Journal Title

      Breeding Science

      Volume: 70 Issue: 1 Pages: 93-100

    • DOI

      10.1270/jsbbs.19028

    • NAID

      130007809746

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gene flow signature in the S-allele region of cultivated buckwheat2019

    • Author(s)
      N. Mizuno, Y. Yasui
    • Journal Title

      BMC Plant Biology

      Volume: 19 Issue: 1 Pages: 125-125

    • DOI

      10.1186/s12870-019-1730-1

    • NAID

      120006623974

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 孤児作物のゲノム遺伝学およびその展望2023

    • Author(s)
      大田竜也 FAWCETT Jeffrey A. 竹島亮馬 菊池真司 大迫敬義 白澤健太 法月美悠 松井勝弘 矢崎裕規 小木曽映里 藤井健一郎 原尚資 JONES Martin K. 平川英樹 LI Cheng-Yun 安井康夫
    • Organizer
      日本進化学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ソバ・ゲノム・プロジェクト-その概要と展望-2023

    • Author(s)
      大田竜也 FAWCETT J. 竹島亮馬 菊池真司 大迫敬義 白澤健太 法月美悠 松井勝弘 矢崎裕規 木曽映里 藤井健一郎 原尚資 JONES Martin K. 平川英樹 LI Cheng-Yun 安井康夫
    • Organizer
      日本育種学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] NGS-TILLINGを利用したモチ性フツウソバの開発2023

    • Author(s)
      安井康夫 FAWCETT J. 田中朋之 西村和紗 西村和紗 中崎鉄也 岩橋優 齊藤大樹 竹内直子 上野まりこ 上野まりこ 白澤健太 平川英樹 大田竜也
    • Organizer
      日本育種学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ソバの異形花型自家不和合性の分子機構とゲノム構造2023

    • Author(s)
      竹島亮馬 , FAWCETT J. , 松井勝弘 , 水野信之 , 松本大生 , 平川英樹 , 大田竜也 , 安井康夫
    • Organizer
      日本育種学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ソバゲノム解読から見えてきたフラボノイド合成制御系2023

    • Author(s)
      松井勝弘 , 大島良美 , 光田展隆 , 坂本真吾 , FAWCETT J. , 平川英樹 , 大田竜也 , 安井康夫
    • Organizer
      日本育種学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ヘミザイガスなゲノム領域によるソバ二花柱性の制御-古典的なSuper geneモデルじゃなかったです-2023

    • Author(s)
      安井康夫 , 竹島亮馬 , FAWCETT Jeffrey , 松井勝弘 , 水野信之 , 松本大生 , 平川英樹 , 白澤健太 , 大田竜也
    • Organizer
      日本分子生物学会年会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] ゲノムシークエンシングは普通ソバにおける異型花柱性の遺伝的構造を明らかにする2023

    • Author(s)
      FAWCETT Jeffrey , 竹島亮馬 , 松井勝弘 , 水野信之 , 松本大生 , 平川英樹 , 大田竜也 , 安井康夫
    • Organizer
      日本遺伝学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] De novoアセンブリとbulked segregant 解析を用いたキヌア本葉の赤色色素生産に関わる遺伝子の同定2022

    • Author(s)
      久篠 沙耶子, 水野 信之, 西村 和紗, 上野 まりこ, 中崎 鉄矢, 小林 安文, 藤田 泰成, 白澤 健太, 平川 英樹, 安井 康夫, 桂 圭佑
    • Organizer
      日本作物学会
    • Related Report
      2022 Research-status Report
  • [Presentation] ソバアナフィラキシーリスクマネジメントに向けた低アレルゲン特性系統の探索と獲得2022

    • Author(s)
      原尚資, 佐藤里絵, 岡本薫, 圓山恭之進, 松井勝弘, 鈴木達郎, 石黒浩二, 大塚しおり, CHEN R., 手島玲子, 近藤康人, 安井康夫
    • Organizer
      日本育種学会
    • Related Report
      2022 Research-status Report
  • [Presentation] ソバ全染色体の塩基配列決定2021

    • Author(s)
      安井康夫
    • Organizer
      育種学会(2021.09.24) ランチタイムセミナー
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] High genetic diversity within cultivated common buckwheat: implication of introgression from wild common buckwheat in China2019

    • Author(s)
      Yasuo Yasui
    • Organizer
      2019年第1回西南四省(自治区)遺伝学学会連合会(中国)
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] ソバ属植物におけるゲノムの進化2019

    • Author(s)
      FAWCETT Jeffrey, 上野まりこ, 大澤良, 大田竜也, 齊藤大樹, 齊藤大樹, 白澤健太, 竹島亮馬, 中崎鉄也, 西村和紗, 原尚資, 原尚資, 平川英樹, 松井勝弘, 水野信之, 安井康夫
    • Organizer
      日本遺伝学会
    • Related Report
      2019 Research-status Report
  • [Presentation] ソバにおける異型花型自家不和合性遺伝子座のゲノム解析2019

    • Author(s)
      大田竜也, 相井城太郎, 上野まりこ, 大澤良, 齊藤大樹, 齊藤大樹, 白澤健太, 竹島亮馬, 中崎鉄也, 西村和紗, 原尚資, 原尚資, 平川英樹, FAWCETT J, 松井勝弘, 水野信之, 安井康夫
    • Organizer
      日本育種学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 研究者単位でのリファレンスゲノムの時代へ ーDeNovoMAGIC を用いたソバのリファレンス配列の作成とその利用についてー2019

    • Author(s)
      安井康夫
    • Organizer
      アグリゲノム産業研究会(招待講演)
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Identification of genes at the S-locus controlling heteromorphic self-incompatibility in buckwheat through genetic and genomic analyses2018

    • Author(s)
      Y. Yasui, N. Mizuno, J. Aii and T. Ota
    • Organizer
      The Society for Molecular Biology & Evolution
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research

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Published: 2018-10-12   Modified: 2025-01-30  

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