Elucidation of the process of membrane protein insrtion and folding.

• Project/Area Number: 18KK0197
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Tsukazaki Tomoya 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (80436716)
• Co-Investigator(Kenkyū-buntansha): 塩田 拓也 宮崎大学, キャリアマネジメント推進機構, 准教授 (20819304) ; 西山 賢一 岩手大学, 農学部, 教授 (80291334)
• Project Period (FY): 2018-10-09 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 膜タンパク質 / タンパク質 / 輸送 / 大腸菌 / 膜タンパク質形成

Outline of Final Research Achievements

In recent years, many pathogenic bacteria gain drug resistance. Bacteria such as Escherichia coli have two membranes, the inner and outer membranes. It is important to study the formation of these membranes both for basic academic research and for the development of new type drugs. We conducted several biochemical analyses of membrane-related factors to uncover the detailed membrane protein biogenesis. In the analysis of environmental changes at the membrane interface by neutron reflectometry, we collaborated collaboration with Dr. Shen at Monash University, Australia.

Academic Significance and Societal Importance of the Research Achievements

病原性大腸菌などグラム陰性菌は、２つの生体膜(内膜・外膜)を持つ。膜には、感染や毒素分泌等に関わる膜蛋白質が局在化しており、防御と攻撃を両立させた環境を形成する。「膜環境形成の分子メカニズムを詳細に理解」するため、生化学的・構造生物学的解析と並行して、新たな国際共同研究として中性子反射率法による膜界面の環境変化の解析を行った。本研究は生命活動の根幹を明らかとするため学術的に意義深いだけでなく、病原性細菌の膜環境形成を阻害する新規抗菌薬開発の基盤となる。

Research Products

• [Int'l Joint Research] モナッシュ大学（Shen研究室, Lithgow研究室）(オーストラリア)
• [Int'l Joint Research] モナシュ大学 Shen研究室/モナシュ大学 Lithgow研究室(オーストリア)
• [Int'l Joint Research] Monash University/Biomedicine Discovery Institute/Department of Microbiology(オーストラリア)
• [Journal Article] Substrate-dependent arrangements of the subunits of the BAM complex determined by neutron reflectometry (2021)
  • Author(s): Chen Xiaoyu、Ding Yue、Bamert Rebecca S.、Le Brun Anton P.、Duff Anthony P.、Wu Chun-Ming、Hsu Hsien-Yi、Shiota Takuya、Lithgow Trevor、Shen Hsin-Hui
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Biomembranes Volume: in press Issue: 9 Pages: 183587-183587
  • DOI: 10.1016/j.bbamem.2021.183587
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ring assembly of c subunits of F0F1‐ATP synthase in Propionigenium modestum requires YidC and UncI following MPIase‐dependent membrane insertion (2021)
  • Author(s): Nishikawa Hanako、Kanno Kotoka、Endo Yuta、Nishiyama Ken‐ichi
  • Journal Title: FEBS Letters Volume: 595 Issue: 5 Pages: 647-654
  • DOI: 10.1002/1873-3468.14036
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Proton-driven bacterial protein translocation motor SecDF (2020)
  • Author(s): 塚崎 智也
  • Journal Title: 生化学 Volume: 92 Issue: 5 Pages: 717-721
  • DOI: 10.14952/SEIKAGAKU.2020.920717
  • NAID: 40022394510
  • ISSN: 0037-1017
  • Year and Date: 2020-10-25
  • Peer Reviewed / Open Access
• [Journal Article] Crystal structure of a YeeE/YedE family protein engaged in thiosulfate uptake (2020)
  • Author(s): Tanaka Yoshiki、Yoshikaie Kunihito、Takeuchi Azusa、Ichikawa Muneyoshi、Mori Tomoyuki、Uchino Sayaka、Sugano Yasunori、Hakoshima Toshio、Takagi Hiroshi、Nonaka Gen、Tsukazaki Tomoya
  • Journal Title: Science Advances Volume: 6 Issue: 35
  • DOI: 10.1126/sciadv.aba7637
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reversible auto-inhibitory regulation of Escherichia coli metallopeptidase BepA for selective β-barrel protein degradation. (2020)
  • Author(s): Daimon 他7名
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 117 Issue: 45 Pages: 27989-27996
  • DOI: 10.1073/pnas.2010301117
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of BamA reconstituted into a solid-supported lipid bilayer as a platform for measuring dynamics during substrate protein assembly into the membrane (2020)
  • Author(s): Ding Yue、Shiota Takuya、Le Brun Anton P.、Dunstan Rhys A.、Wang Bo、Hsu Hsien-Yi、Lithgow Trevor、Shen Hsin-Hui
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Biomembranes Volume: 1862 Issue: 9 Pages: 183317-183317
  • DOI: 10.1016/j.bbamem.2020.183317
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biosynthesis of glycolipid MPIase (membrane protein integrase) is independent of the genes for ECA (enterobacterial common antigen) (2020)
  • Author(s): Kamemoto Yuki、Funaba Nanaka、Kawakami Mayu、Sawasato Katsuhiro、Kanno Kotoka、Suzuki Sonomi、Nishikawa Hanako、Sato Ryo、Nishiyama Ken-ichi
  • Journal Title: The Journal of General and Applied Microbiology Volume: 66 Issue: 3 Pages: 169-174
  • DOI: 10.2323/jgam.2019.05.001
  • NAID: 130007891119
  • ISSN: 0022-1260, 1349-8037
  • Peer Reviewed / Open Access
• [Journal Article] In vitro Assay for Bacterial Membrane Protein Integration into Proteoliposomes (2020)
  • Author(s): Hanako Nishikawa, Masaru Sasaki, Ken-ichi Nishiyama
  • Journal Title: Bio-protocol Volume: 10 Issue: 10
  • DOI: 10.21769/bioprotoc.3626
  • Peer Reviewed / Open Access
• [Journal Article] 大腸菌におけるタンパク質膜輸送に関与する糖脂質MPIase の発現制御機構~酵素様機能をもつ糖脂質 MPIase~ (2020)
  • Author(s): 沢里克宏, 藤川紘樹, 島本啓子, 西山賢一
  • Journal Title: 化学と生物 Volume: 58 Pages: 223-230
  • NAID: 130008013958
  • Peer Reviewed
• [Journal Article] Enzyme-like Glycolipids MPIase Involved in Membrane Protein Integration of <i>E. coli</i> (2019)
  • Author(s): Kohki Fujikawa, Ken-ichi Nishiyama and Keiko Shimamoto
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 31 Issue: 184 Pages: E151-E158
  • DOI: 10.4052/tigg.1705.1E
  • NAID: 130007751014
  • ISSN: 0915-7352, 1883-2113
  • Year and Date: 2019-11-25
  • Peer Reviewed / Open Access
• [Journal Article] Enzyme-like Glycolipids MPIase Involved in Membrane Protein Integration of <i>E. coli</i> (2019)
  • Author(s): 藤川 紘樹、西山 賢一、島本 啓子-
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 31 Issue: 184 Pages: J149-J155
  • DOI: 10.4052/tigg.1705.1J
  • NAID: 130007751011
  • ISSN: 0915-7352, 1883-2113
  • Year and Date: 2019-11-25
  • Peer Reviewed / Open Access
• [Journal Article] Novel Glycolipid Involved in Membrane Protein Integration: Structure and Mode of Action (2019)
  • Author(s): Fujikawa, K.; Nomura, K.; Nishiyama, K. I.; Shimamoto, K.
  • Journal Title: Journal of Synthetic Organic Chemistry, Japan Volume: 77 Issue: 11 Pages: 1096-1105
  • DOI: 10.5059/yukigoseikyokaishi.77.1096
  • NAID: 130007742536
  • ISSN: 0037-9980, 1883-6526
  • Year and Date: 2019-11-01
  • Peer Reviewed / Open Access
• [Journal Article] Structure of mitochondrial import gate reveals distinct preprotein paths. (2019)
  • Author(s): Araiso, Y., Tsutsumi, A., Qui, J., Imai, K., Shiota, T., Song, J., Lindau, C., Wenz, LS., Sakaue,H., Yunoki, K., Kawano, S., Suzuki, J., Wichnewski, M., Schutze, C., Ariyama, H., Ando, T., Becker, T., Lithgow, T., Weidemann, N., Pfanner, N., Kikkawa, M., Endo, T.
  • Journal Title: Nature Volume: 575 Issue: 7782 Pages: 395-401
  • DOI: 10.1038/s41586-019-1680-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural basis of sarco/endoplasmic reticulum Ca2+-ATPase 2b regulation via transmembrane helix interplay (2019)
  • Author(s): Michio Inoue, Nanami Sakuta, Satoshi Watanabe, Yuxia Zhang, Kunihito Yoshikaie, Yoshiki Tanaka, Ryo Ushioda, Yukinari Kato, Junichi Takagi, Tomoya Tsukazaki, Kazuhiro Nagata, Kenji Inaba
  • Journal Title: Cell Reports Volume: 27 Issue: 4 Pages: 1221-1230
  • DOI: 10.1016/j.celrep.2019.03.106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural Basis of the Sec Translocon and YidC Revealed Through X-ray Crystallography (2019)
  • Author(s): Tsukazaki Tomoya
  • Journal Title: The Protein Journal Volume: - Issue: 3 Pages: 249-261
  • DOI: 10.1007/s10930-019-09830-x
  • NAID: 120006718071
  • Peer Reviewed / Open Access
• [Journal Article] A snapshot of membrane protein insertion (2019)
  • Author(s): Tanaka Yoshiki、Tsukazaki Tomoya
  • Journal Title: EMBO reports Volume: 20 Issue: 10
  • DOI: 10.15252/embr.201949034
  • Peer Reviewed / Open Access
• [Journal Article] The bacterial protein YidC accelerates MPIase-dependent integration of membrane proteins (2019)
  • Author(s): Sasaki Masaru、Nishikawa Hanako、Suzuki Sonomi、Moser Michael、Huber Maria、Sawasato Katsuhiro、Matsubayashi Hideaki T.、Kumazaki Kaoru、Tsukazaki Tomoya、Kuruma Yutetsu、Nureki Osamu、Ueda Takuya、Nishiyama Ken-ichi
  • Journal Title: Journal of Biological Chemistry Volume: 294 Issue: 49 Pages: 18898-18908
  • DOI: 10.1074/jbc.ra119.011248
  • Peer Reviewed / Open Access
• [Journal Article] Real-time observation of membrane protein-embedded nanodiscs by high-speed atomic force microscopy (2019)
  • Author(s): Takamitsu Haruyama, Tomoya Tsukazaki
  • Journal Title: 野口研究所時報 Volume: 62 Pages: 42-49
  • NAID: 40022065376
  • Open Access
• [Journal Article] Free energy profiles of lipid translocation across pure POPC and POPC/CHOL bilayer: all-atom molecular dynamics study (2019)
  • Author(s): Saito Hiroaki、Morishita Tetsuya、Mizukami Taku、Nishiyama Ken-ichi、Kawaguchi Kazutomo、Nagao Hidemi
  • Journal Title: Journal of Physics: Conference Series Volume: 1290 Issue: 1 Pages: 012020-012020
  • DOI: 10.1088/1742-6596/1290/1/012020
  • Peer Reviewed / Open Access
• [Journal Article] Alteration of membrane physicochemical properties by two factors for membrane protein integration (2019)
  • Author(s): Nomura K, Yamaguchi T, Mori S, Fujikawa K, Nishiyama K, Shimanouchi T, Tanimoto Y, Morigaki K, Shimamoto K.
  • Journal Title: Biophys. J. Volume: 117 Issue: 1 Pages: 99-110
  • DOI: 10.1016/j.bpj.2019.05.014
  • NAID: 120006844183
  • Peer Reviewed / Open Access
• [Journal Article] Two‐step induction of cdsA promoters leads to upregulation of the glycolipid MPIase at cold temperature (2019)
  • Author(s): Sawasato Katsuhiro、Sekiya Yusei、Nishiyama Ken‐ichi
  • Journal Title: FEBS Letters Volume: 593 Issue: 14 Pages: 1711-1723
  • DOI: 10.1002/1873-3468.13460
  • Peer Reviewed / Open Access
• [Journal Article] Expression of alcohol oxidase gene from Ochrobactrum sp. AIU 033 in recombinant Escherichia coli through the twin-arginine translocation pathway (2019)
  • Author(s): Matsumura Kenji、Yamada Miwa、Yamashita Takeshi、Muto Hitomi、Nishiyama Ken-ichi、Shimoi Hitoshi、Isobe Kimiyasu
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 128 Issue: 1 Pages: 13-21
  • DOI: 10.1016/j.jbiosc.2018.12.012
  • NAID: 40021953936
  • Peer Reviewed / Open Access
• [Journal Article] Increased expression of the bacterial glycolipid MPIase is required for efficient protein translocation across membranes in cold conditions (2019)
  • Author(s): Sawasato Katsuhiro、Suzuki Sonomi、Nishiyama Ken-ichi
  • Journal Title: Journal of Biological Chemistry Volume: 294 Issue: 21 Pages: 8403-8411
  • DOI: 10.1074/jbc.ra119.008457
  • Peer Reviewed / Open Access
• [Journal Article] YnbB is a CdsA paralogue dedicated to biosynthesis of glycolipid MPIase involved in membrane protein integration (2019)
  • Author(s): Sato Ryo、Sawasato Katsuhiro、Nishiyama Ken-ichi
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 510 Issue: 4 Pages: 636-642
  • DOI: 10.1016/j.bbrc.2019.01.145
  • Peer Reviewed / Open Access
• [Journal Article] CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo (2019)
  • Author(s): Sawasato Katsuhiro、Sato Ryo、Nishikawa Hanako、Iimura Naoki、Kamemoto Yuki、Fujikawa Kohki、Yamaguchi Toshiyuki、Kuruma Yutetsu、Tamura Yasushi、Endo Toshiya、Ueda Takuya、Shimamoto Keiko、Nishiyama Ken-ichi
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 1372-1372
  • DOI: 10.1038/s41598-018-37809-8
  • NAID: 120007176808
  • Peer Reviewed / Open Access
• [Journal Article] Structural basis of the function of the β-barrel assembly-enhancing protease BepA (2019)
  • Author(s): Mohammad Shahrizal, Yasushi Daimon, Yoshiki Tanaka, Yugo Hayashi, Shintaro Nakayama, Shigehiro Iwaki, Shin-ichiro Narita, Hironari Kamikubo, Yoshinori Akiyama, Tomoya Tsukazaki
  • Journal Title: Journal of Molecular Biology Volume: 431 Issue: 3 Pages: 625-635
  • DOI: 10.1016/j.jmb.2018.11.024
  • Peer Reviewed / Open Access
• [Journal Article] Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy (2019)
  • Author(s): Haruyama, T. Sugano, Y. Kodera, N. Uchihashi, T. Ando, T. Tanaka, Y. Konno, H. Tsukazaki, T.
  • Journal Title: Structure Volume: 27 Issue: 1 Pages: 152-160
  • DOI: 10.1016/j.str.2018.09.005
  • Peer Reviewed / Open Access
• [Journal Article] Porin Associates with Tom22 to Regulate the Mitochondrial Protein Gate Assembly (2019)
  • Author(s): Sakaue Haruka、Shiota Takuya、Ishizaka Naoya、Kawano Shin、Tamura Yasushi、Tan Kher Shing、Imai Kenichiro、Motono Chie、Hirokawa Takatsugu、Taki Kentaro、Miyata Non、Kuge Osamu、Lithgow Trevor、Endo Toshiya
  • Journal Title: Molecular Cell Volume: 73 Issue: 5 Pages: 1044-1055
  • DOI: 10.1016/j.molcel.2019.01.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Free energy profiles of lipid translocation across pure POPC and POPC/CHOL bilayer : all - atom molecular dynamics study (2019)
  • Author(s): Saito Hiroaki、Morishita Tetsuya、Mizukami Taku、Nishiyama Ken-ichi、Kawaguchi Kazutomo、Nagao Hidemi
  • Journal Title: Journal of Physics: Conference Series Volume: -
  • Peer Reviewed / Open Access
• [Journal Article] 2.8-A crystal structure of Escherichia coli YidC revealing all core regions, including flexible C2 loop (2018)
  • Author(s): Tanaka Yoshiki、Izumioka Akiya、Abdul Hamid Aisyah、Fujii Akira、Haruyama Takamitsu、Furukawa Arata、Tsukazaki Tomoya
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 505 Issue: 1 Pages: 141-145
  • DOI: 10.1016/j.bbrc.2018.09.043
  • Peer Reviewed / Open Access
• [Journal Article] Structure-based working model of SecDF, a proton-driven bacterial protein translocation factor (2018)
  • Author(s): Tsukazaki Tomoya
  • Journal Title: FEMS Microbiology Letters Volume: 365 Issue: 12
  • DOI: 10.1093/femsle/fny112
  • NAID: 120006537408
  • Peer Reviewed / Open Access
• [Journal Article] Remote Coupled Drastic β-Barrel to β-Sheet Transition of the Protein Translocation Motor. (2018)
  • Author(s): Furukawa A, Nakayama S, Yoshikaie K, Tanaka Y, Tsukazaki T.
  • Journal Title: Structure Volume: 26 Issue: 3 Pages: 485-489
  • DOI: 10.1016/j.str.2018.01.002
  • Peer Reviewed / Open Access
• [Journal Article] Cholesterol blocks spontaneous insertion of membrane proteins into liposomes of phosphatidylcholine (2018)
  • Author(s): Shota Nakamura, Sonomi Suzuki, Hiroaki Saito and Ken-ichi Nishiyama
  • Journal Title: The Journal of Biochemistry Volume: 163 Issue: 4 Pages: 313-319
  • DOI: 10.1093/jb/mvx083
  • NAID: 40021524045
  • Peer Reviewed / Open Access
• [Journal Article] Molecular dynamics study of binary POPC bilayers: molecular condensing effects on membrane structure and dynamics (2018)
  • Author(s): Saito Hiroaki、Morishita Tetsuya、Mizukami Taku、Nishiyama Ken-ichi、Kawaguchi Kazutomo、Nagao Hidemi
  • Journal Title: Journal of Physics: Conference Series Volume: 1136 Pages: 012022-012022
  • DOI: 10.1088/1742-6596/1136/1/012022
  • Peer Reviewed / Open Access
• [Journal Article] Syntheses and Activities of the Functional Structures of a Glycolipid Essential for Membrane Protein Integration (2018)
  • Author(s): Fujikawa Kohki、Suzuki Sonomi、Nagase Ryohei、Ikeda Shiori、Mori Shoko、Nomura Kaoru、Nishiyama Ken-ichi、Shimamoto Keiko
  • Journal Title: ACS Chemical Biology Volume: 13 Issue: 9 Pages: 2719-2727
  • DOI: 10.1021/acschembio.8b00654
  • Peer Reviewed / Open Access
• [Presentation] 高速原子間顕微鏡による膜輸送装置Secの動態観察 (2021)
  • Author(s): 長池航，板家成良，春山隆充，塚﨑智也，内橋貴之
  • Organizer: 令和2年度中部支部講演会および総会
• [Presentation] 高速原子間力顕微鏡によるタンパク質膜輸送装置Secの動態観察 (2020)
  • Author(s): 長池航，板家成良，春山隆充，塚崎智也，内橋貴之
  • Organizer: 日本生体エネルギー研究会第46回討論会
• [Presentation] Observation of Substrate Binding Sec Translocon and Structural Change of SecA with HS-AFM (2020)
  • Author(s): 長池航，春山隆充，塚崎智也，内橋貴之
  • Organizer: The 58th Annual Meeting of the BSJ
  • Int'l Joint Research
• [Presentation] タンパク質膜挿入・膜透過に必須の糖脂質MPIaseの構造と機能 (2020)
  • Author(s): 西山賢一
  • Organizer: 2020年度東北支部シンポジウム「いまホットな脂質研究」
  • Invited
• [Presentation] バクテリア外膜タンパク質アセンブリー解析の新手法「EMMアセンブリーアッセイ」 (2020)
  • Author(s): 塩田拓也
  • Organizer: 第93回 日本細菌学会総会
  • Invited
• [Presentation] タンパク質膜挿入・膜透過反応に関与する「糖脂質酵素(Glycolipozyme)」MPIaseの構造と機能 (2020)
  • Author(s): 西山賢一
  • Organizer: 新潟薬科大学学術講演会
  • Invited
• [Presentation] 大腸菌のタンパク質膜挿入・膜透過にかかわる糖脂質MPIaseの構造と機能 (2020)
  • Author(s): 西山賢一
  • Organizer: 遺伝研研究会「単細胞システムにおける細胞内装置の動的相互作用」
  • Invited
• [Presentation] 硫黄トランスポーターの結晶構造解析 (2019)
  • Author(s): 塚崎智也
  • Organizer: 第16回21世紀大腸菌研究会
• [Presentation] タンパク質膜透過チャネルSecトランスロコンを経由するタンパク質輸送のメカニズム (2019)
  • Author(s): 塚崎智也
  • Organizer: 岩手大学放射光利用セミナー
  • Invited
• [Presentation] 大腸菌外膜βバレル型膜タンパク質輸送阻害ペプチドの探索 (2019)
  • Author(s): 塩田拓也
  • Organizer: 第16回21世紀大腸菌研究会
• [Presentation] Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs by High-Speed Atomic Force Microscopy (2019)
  • Author(s): 塚崎智也
  • Organizer: EMBO Workshop: CURRENT ADVANCES IN PROTEIN TRANSLOCATION ACROSS MEMBRANES
  • Int'l Joint Research
• [Presentation] EMM アセンブリーアッセイを用いた外膜タンパク質アセンブリーの分子メカニズム解析 (2019)
  • Author(s): 塩田拓也
  • Organizer: 2018年度遺伝研研究会
  • Invited
• [Presentation] Analysis of the molecular mechanism of E. coli BAM complex by new method "EMM assembly assay (2019)
  • Author(s): 塩田拓也
  • Organizer: EMBO Workshop Current advances in protein translocation across membranes
  • Int'l Joint Research
• [Presentation] タンパク質膜挿入・膜透過に関与する糖脂質MPIaseの構造と機能 (2019)
  • Author(s): 西山賢一
  • Organizer: 理学部講演会(山形大学)
  • Invited
• [Presentation] タンパク質膜挿入・膜透過に関与する糖脂質MPIase の構造と機能 (2019)
  • Author(s): 西山賢一
  • Organizer: 第1回細胞形成研究会
  • Invited
• [Presentation] Working model of SecDF inferred from structural studies (2018)
  • Author(s): 塚崎智也
  • Organizer: Joint Symposium ~ The 10th symposium on International Collaborative Laboratories ~ BioTechnology Institute, U. Minnesota & Grad School BioScience, NAIST
  • Int'l Joint Research / Invited
• [Presentation] Drastic conformational changes of SecDF (2018)
  • Author(s): 塚崎智也
  • Organizer: BACTERIAL PROTEIN EXPORT 2018
  • Int'l Joint Research
• [Presentation] High-speed AFM imaging of membrane protein embedded in Nanodisc (2018)
  • Author(s): 塚崎智也
  • Organizer: 第56回日本生物物理学会年会
  • Int'l Joint Research
• [Presentation] 結晶構造解析から明らかとなってきたタンパク質膜透過モーター SecDF の劇的な構造変化 (2018)
  • Author(s): 塚崎智也
  • Organizer: 第18回日本蛋白質科学会年会
• [Presentation] Cell cycle-dependent dynamic association of the mitochondrial protein entry gate, TOM complex (2018)
  • Author(s): 塩田拓也
  • Organizer: 第41回日本分子生物学会年会
  • Invited
• [Presentation] Increase in the expression level of Glycolipozyme MPIase is required for the efficient protein translocation in the cold (2018)
  • Author(s): 西山賢一
  • Organizer: Bacterial Protein Export 2018
  • Int'l Joint Research
• [Presentation] Structure and function of MPIase involved in membrane protein integration and preprotein translocation (2018)
  • Author(s): 西山賢一
  • Organizer: BMB Seminar (Freiburg University)
  • Int'l Joint Research / Invited
• [Book] 膜タンパク質工学ハンドブック (2020)
  • Author(s): 浜窪隆雄, 津本浩平
  • Total Pages: 538
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