Neural regulatory mechanism of memory formation and erasure during sleep

• Project/Area Number: 18KK0223
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 48:Biomedical structure and function and related fields
• Research Institution: Nagoya University
• Principal Investigator: 山中 章弘 名古屋大学, 環境医学研究所, 教授 (60323292)
• Co-Investigator(Kenkyū-buntansha): 山下 貴之 名古屋大学, 環境医学研究所, 客員教授 (40466321) ; 山下 哲 鹿児島大学, 医歯学域医学系, 助教 (40740197)
• Project Period (FY): 2018-10-09 – 2022-03-31
• Project Status: Discontinued (Fiscal Year 2021)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2023: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2022: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2021: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: メラニン凝集ホルモン / 視床下部 / カルシウムイメージング / 光遺伝学 / 活動記録 / GCaMP6 / 遺伝子改変動物 / アデノ随伴ウイルス / メラニン凝集ホルモン産生神経 / アセチルコリン作動性神経 / 記憶 / 脳スライス標本 / 睡眠覚醒 / レム睡眠 / メラニン凝集ホルモン神経 / 海馬 / 化学遺伝学 / MCH神経 / マイクロエンドスコープ / 睡眠 / ウイルスベクター / 記憶消去

Outline of Annual Research Achievements

メラニン凝集ホルモン(MCH)神経の生理的役割解明のために、光で神経活動を操作可能にする光遺伝学を適用可能な遺伝子改変マウスを独自に作出した。このマウスを用いて、MCH神経を光で活性化させたところ、レム睡眠時間が約3倍に増加することを見いだした。MCH神経を時期特異的に脱落可能な遺伝子改変マウスを作成し、睡眠覚醒調節への影響を解析した。しかし、これらのマウスでは期待されたレム睡眠時間の減少は認められなかった。このことは、MCH神経は、レム睡眠の開始などには必須ではなく、レム睡眠時に別の生理機能を担っている可能性を示唆していた。組織化学的解析からMCH神経が記憶に重要な海馬に密に投射していることを見いだしたため、レム睡眠時の記憶制御に関わっている可能性が考えられた。そこで、新奇物体認識試験や、水迷路を用いてMCH神経脱落マウスの認識記憶や空間学習記憶を評価したところ、MCH神経脱落マウスの記憶と保持が対照群マウスと比べていずれの試験においても有意に向上していることを見いだした。脱落とは逆に光遺伝学や、薬理遺伝学を用いてMCH神経を活性化すると、記憶が抑制・消失された。このことは、MCH神経活動が記憶抑制・消去に働いていることを示している。そこで、MCH神経の活動についてSRIの共同研究者であるKilduff博士と超小型内視鏡を用いて記録し、解析したところ、MCH神経には覚醒時に活動するタイプ、レム睡眠時に活動するタイプ、覚醒時とレム睡眠時の両方で活動する3タイプが認められた。光遺伝学を用いて、脳状態依存的なMCH神経活動の抑制を行ったところ、レム睡眠時に抑制した時だけに記憶が向上した。これらの結果はレム睡眠時に活動するMCH神経が記憶消去に関わっていることを示している（Izawa et al., Science 2019）。

Research Products

• [Int'l Joint Research] SRI International(米国)
• [Int'l Joint Research] SRI International(米国)
• [Int'l Joint Research] SRI International(米国)
• [Journal Article] Involvement of A5/A7 noradrenergic neurons and B2 serotonergic neurons in nociceptive processing: a fiber photometry study. (2022)
  • Author(s): Moriya S, Yamashita A, Masukawa D, Sakaguchi J, Ikoma Y, Sameshima Y, Kambe Y, Yamanaka A, *Kuwaki T.
  • Journal Title: Neural Regen Res Volume: 17(4) Issue: 4 Pages: 881-886
  • DOI: 10.4103/1673-5374.322465
  • Peer Reviewed / Open Access
• [Journal Article] Melanin-concentrating hormone-producing neurons in the hypothalamus regulate brown adipose tissue and thus contribute to energy expenditure. (2022)
  • Author(s): Izawa S., Yoneshiro T., Kondoh K., Nakagiri S., Okamatsu-Ogura Y., Terao A., Minokoshi Y., Yamanaka A., and Kimura K.
  • Journal Title: J. Physiol. Volume: 600 Issue: 4 Pages: 815-827
  • DOI: 10.1113/jp281241
  • Peer Reviewed / Open Access
• [Journal Article] The Development of Sleep/Wake Disruption and Cataplexy as Hypocretin/Orexin Neurons Degenerate in Male vs. Female Orexin/tTA; TetO-DTA Mice. (2022)
  • Author(s): Sun Y, Tisdale R, Park S, Ma SC, Heu J, Haire M, Allocca G, Yamanaka A, Morairty SR, *Kilduff TS.
  • Journal Title: Sleep Volume: 19 Issue: 12
  • DOI: 10.1093/sleep/zsac039
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Conditional Knockout of Bmal1 in Corticotropin-Releasing Factor Neurons Does Not Alter Sleep-Wake Rhythm in Mice. (2022)
  • Author(s): Hung CJ, Yamanaka A, *Ono D.
  • Journal Title: Front Neurosci Volume: 15 Pages: 808754-808754
  • DOI: 10.3389/fnins.2021.808754
  • Peer Reviewed / Open Access
• [Journal Article] Activity of putative orexin neurons during cataplexy (2022)
  • Author(s): Zhou Shi、Yamashita Akira、Su Jingyang、Zhang Yang、Wang Wuyang、Hao Liying、Yamanaka Akihiro、Kuwaki Tomoyuki
  • Journal Title: Molecular Brain Volume: 15 Issue: 1 Pages: 21-21
  • DOI: 10.1186/s13041-022-00907-w
  • Peer Reviewed / Open Access
• [Journal Article] Remote control of neural function by X-ray-induced scintillation (2021)
  • Author(s): Matsubara Takanori、Yanagida Takayuki、Kawaguchi Noriaki、Nakano Takashi、Yoshimoto Junichiro、Sezaki Maiko、Takizawa Hitoshi、Tsunoda Satoshi P.、Horigane Shin-ichiro、Ueda Shuhei、Takemoto-Kimura Sayaka、Kandori Hideki、Yamanaka Akihiro、Yamashita Takayuki
  • Journal Title: Nature Communications Volume: 12 Issue: 1 Pages: 4478-4478
  • DOI: 10.1038/s41467-021-24717-1
  • Peer Reviewed / Open Access
• [Journal Article] Aversive emotion rapidly activates orexin neurons and increases heart rate in freely moving mice (2021)
  • Author(s): Yamashita Akira、Moriya Shunpei、Nishi Ryusei、Kaminosono Jun、Yamanaka Akihiro、Kuwaki Tomoyuki
  • Journal Title: Molecular Brain Volume: 14 Issue: 1 Pages: 104-120
  • DOI: 10.1186/s13041-021-00818-2
  • Peer Reviewed / Open Access
• [Journal Article] Disruption of model-based decision making by silencing of serotonin neurons in the dorsal raphe nucleus (2021)
  • Author(s): Ohmura Yu、Iwami Kentaro、Chowdhury Srikanta、Sasamori Hitomi、Sugiura Chiaki、Bouchekioua Youcef、Nishitani Naoya、Yamanaka Akihiro、 Yoshioka Mitsuhiro
  • Journal Title: Current Biology Volume: 31 Issue: 11 Pages: 1-9
  • DOI: 10.1016/j.cub.2021.03.048
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Hypocretin/Orexin Interactions with Norepinephrine Contribute to the Opiate Withdrawal Syndrome. (2021)
  • Author(s): McGregor R, Wu MF, Holmes B, Lam HA, Maidment NT, Gera J, Yamanaka A, *Siegel JM.
  • Journal Title: J Neurosci Volume: 42(2) Issue: 2 Pages: 255-263
  • DOI: 10.1523/jneurosci.1557-21.2021
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Hypothalamic perifornical Urocortin-3 neurons modulate defensive responses to a potential threat stimulus (2021)
  • Author(s): Horii-Hayashi Noriko、Nomoto Kensaku、Endo Nozomi、Yamanaka Akihiro、Kikusui Takefumi、Nishi Mayumi
  • Journal Title: iScience Volume: 24 Issue: 1 Pages: 101908-101908
  • DOI: 10.1016/j.isci.2020.101908
  • Peer Reviewed / Open Access
• [Journal Article] Direct evidence that the brain reward system is involved in the control of scratching behaviors induced by acute and chronic itch (2021)
  • Author(s): Setsu T, Hamada Y, Oikawa D, Mori T, Ishiuji Y, Sato D, Narita M, Miyazaki S, Furuta E, Suda Y, Sakai H, Ochiya T, Tezuka H, Iseki M, Inada E, Yamanaka A, Kuzumaki N, Narita M.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 534 Pages: 624-631
  • DOI: 10.1016/j.bbrc.2020.11.030
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of MCH-oxytocin neural relay within the hypothalamus in murine nursing behavior (2021)
  • Author(s): Kato Yoko、Katsumata Harumi、Inutsuka Ayumu、Yamanaka Akihiro、Onaka Tatsushi、Minami Shiro、Orikasa Chitose
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 1-12
  • DOI: 10.1038/s41598-021-82773-5
  • Peer Reviewed / Open Access
• [Journal Article] The impacts of age and sex in a mouse model of childhood narcolepsy. (2021)
  • Author(s): Coffey AA, Joyal AA, Yamanaka A, *Scammell TE.
  • Journal Title: Front Neurosci Volume: 15 Pages: 644757-644757
  • DOI: 10.3389/fnins.2021.644757
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Involvement of A13 dopaminergic neurons located in the zona incerta in nociceptive processing: a fiber photometry study (2020)
  • Author(s): Shunpei Moriya, Akira Yamashita, Daiki Masukawa, Honami Setoyama, Yunsu Hwang, Akihiro Yamanaka, Tomoyuki Kuwaki
  • Journal Title: Molecular Brain Volume: 13 Issue: 1 Pages: 1415-1416
  • DOI: 10.1186/s13041-020-00600-w
  • Peer Reviewed / Open Access
• [Journal Article] Dual Orexin and MCH neuron-ablated mice display severe sleep attacks and cataplexy. (2020)
  • Author(s): Hung, J.C., Ono, D., Kilduff, S.T., and Yamanaka, A.
  • Journal Title: eLife Volume: なし Pages: 9-9
  • DOI: 10.7554/elife.54275
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Orexin signaling in GABAergic lateral habenula neurons modulates aggressive behavior in male mice (2020)
  • Author(s): Flanigan ME, Aleyasin H, Li L, Burnett CJ, Chan KL, LeClair KB, Lucas EK, Matikainen-Ankney B, Cuttoli RD, Takahashi A, Menard C, Pfau ML, Golden SA, Bouchard S, Calipari ES, Nestler EJ, DiLeone RJ, Yamanaka A, Huntley GW, Clem RL, Russo SJ
  • Journal Title: Nature Neuroscience Volume: - Issue: 5 Pages: 638-650
  • DOI: 10.1038/s41593-020-0617-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] VGLUT2-expressing neurons in the vestibular nuclear complex mediate gravitational stress-induced hypothermia in mice (2020)
  • Author(s): Abe C, Yamaoka Y, Maejima Y, Mikami T, Yokota S, Yamanaka A, Morita H
  • Journal Title: Communications Biology Volume: 227 Issue: 1 Pages: 1-13
  • DOI: 10.1038/s42003-020-0950-0
  • Peer Reviewed / Open Access
• [Journal Article] Opposing Ventral Striatal Medium Spiny Neuron Activities Shaped by Striatal Parvalbumin-Expressing Interneurons during Goal-Directed Behaviors. (2020)
  • Author(s): Yoshida K, Tsutsui-Kimura I, Kono A, Yamanaka A, Kobayashi K, Watanabe M, Mimura M, *Tanaka KF.
  • Journal Title: Cell Rep Volume: 31 Issue: 13 Pages: 107829-107829
  • DOI: 10.1016/j.celrep.2020.107829
  • Peer Reviewed / Open Access
• [Journal Article] Green-Sensitive, Long-Lived, Step-Functional Anion Channelrhodopsin-2 Variant as a High-Potential Neural Silencing Tool (2020)
  • Author(s): Kojima Keiichi、Miyoshi Natsuki、Shibukawa Atsushi、Chowdhury Srikanta、Tsujimura Masaki、Noji Tomoyasu、Ishikita Hiroshi、Yamanaka Akihiro、Sudo Yuki
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 11 Issue: 15 Pages: 6214-6218
  • DOI: 10.1021/acs.jpclett.0c01406
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of substances which regulate activity of corticotropin-releasing factor-producing neurons in the paraventricular nucleus of the hypothalamus. (2020)
  • Author(s): Mukai Y, Nagayama A, Itoi K, *Yamanaka A.
  • Journal Title: Sci Rep Volume: 10 Issue: 1 Pages: 13639-13639
  • DOI: 10.1038/s41598-020-70481-5
  • Peer Reviewed / Open Access
• [Journal Article] The mammalian circadian pacemaker regulates wakefulness via CRF neurons in the paraventricular nucleus of the hypothalamus. (2020)
  • Author(s): *Ono D, Mukai Y, Hung CJ, Chowdhury S, Sugiyama T, *Yamanaka A.
  • Journal Title: Sci Adv Volume: 6(45) Issue: 45 Pages: 0384-0384
  • DOI: 10.1126/sciadv.abd0384
  • Peer Reviewed / Open Access
• [Journal Article] Serotonergic projections to the orbitofrontal and medial prefrontal cortices differentially modulate waiting for future rewards (2020)
  • Author(s): Miyazaki K, Miyazaki KW, Sivori G, Yamanaka A, Tanaka KF, Doya K
  • Journal Title: Science Advances Volume: 6 Issue: 48 Pages: 7246-7246
  • DOI: 10.1126/sciadv.abc7246
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Animal Models of Narcolepsy and the Hypocretin/Orexin System: Past, Present, and Future. (2020)
  • Author(s): Tisdale RK, Yamanaka A, *Kilduff TS.
  • Journal Title: Sleep Volume: - Issue: 6
  • DOI: 10.1093/sleep/zsaa278
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Accumbal D2R-medium spiny neurons regulate aversive behaviors through PKA-Rap1 pathway. (2020)
  • Author(s): Lin, Y-H., Yamahashi, Y., Kuroda, K., Faruk M. O., Zhang, X., Yamada, K., Yamanaka, A., Nagai, T., Kaibuchi, K.
  • Journal Title: Neurocham. Int. Volume: 143 Pages: 104935-104935
  • DOI: 10.1016/j.neuint.2020.104935
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Different roles of distinct serotonergic pathways in anxiety-like behavior, antidepressant-like, and anti-impulsive effects (2020)
  • Author(s): Ohmura Yu、Tsutsui-Kimura Iku、Sasamori Hitomi、Nebuka Mao、Nishitani Naoya、Tanaka Kenji F.、Yamanaka Akihiro、Yoshioka Mitsuhiro
  • Journal Title: Neuropharmacology Volume: 167 Pages: 107703-107703
  • DOI: 10.1016/j.neuropharm.2019.107703
  • Peer Reviewed
• [Journal Article] Acute restraint stress augments the rewarding memory of cocaine through activation of α1 adrenoceptors in the medial prefrontal cortex of mice. (2020)
  • Author(s): Wada, S., Yanagida, J., Sasase, H., Zhang, T., Li, X., Kamii, H., Domoto, M., Deyama, S., Hinoi, E., Yamanaka, A., Nishitani, N., Nagayasu, K., Kaneko, S., Minami, M., Kaneda, K.
  • Journal Title: Neuropharmacology Volume: 166 Pages: 107968-107968
  • DOI: 10.1016/j.neuropharm.2020.107968
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of supralemniscal nucleus (B9) 5-HT neuronal system in nociceptive processing: a fiber photometry study (2020)
  • Author(s): Moriya Shunpei、Yamashita Akira、Masukawa Daiki、Kambe Yuki、Sakaguchi Junichi、Setoyama Honami、Yamanaka Akihiro、Kuwaki Tomoyuki
  • Journal Title: Molecular Brain Volume: 13 Issue: 1 Pages: 14-14
  • DOI: 10.1186/s13041-020-0553-1
  • Peer Reviewed / Open Access
• [Journal Article] Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3. (2020)
  • Author(s): Imayoshi I, Tabuchi S, Matsumoto M, Kitano S, Miyachi H, Kageyama R, Yamanaka A
  • Journal Title: Sci Rep Volume: 10(1) Issue: 1 Pages: 3191-3191
  • DOI: 10.1038/s41598-020-59984-3
  • NAID: 120006878490
  • Peer Reviewed / Open Access
• [Journal Article] Behavioral characteristics of 5-HT2C receptor knockout mice: Locomotor activity, anxiety-, and fear memory-related behaviors (2020)
  • Author(s): Nebuka Mao、Ohmura Yu、Izawa Shuntaro、Bouchekioua Youcef、Nishitani Naoya、Yoshida Takayuki、Yoshioka Mitsuhiro
  • Journal Title: Behavioural Brain Research Volume: 379 Pages: 112394-112394
  • DOI: 10.1016/j.bbr.2019.112394
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion (2020)
  • Author(s): Nagai Yuma、Takayama Kaito、Nishitani Naoya、Andoh Chihiro、Koda Masashi、Shirakawa Hisashi、Nakagawa Takayuki、Nagayasu Kazuki、Yamanaka Akihiro、Kaneko Shuji
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 6 Pages: 2160-2160
  • DOI: 10.3390/ijms21062160
  • NAID: 120006826701
  • Peer Reviewed / Open Access
• [Journal Article] GABA neurons in the ventral tegmental area regulate non-rapid eye movement sleep in mice (2019)
  • Author(s): Chowdhury Srikanta、Matsubara Takanori、Miyazaki Toh、Ono Daisuke、Fukatsu Noriaki、Abe Manabu、Sakimura Kenji、Sudo Yuki、Yamanaka Akihiro
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.44928
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dissociating orexin-dependent and -independent functions of orexin neurons using novel Orexin-Flp knock-in mice (2019)
  • Author(s): Chowdhury Srikanta、Hung Chi Jung、Izawa Shuntaro、Inutsuka Ayumu、Kawamura Meiko、Kawashima Takashi、Bito Haruhiko、Imayoshi Itaru、Abe Manabu、Sakimura Kenji、Yamanaka Akihiro
  • Journal Title: eLife Volume: 8 Pages: 1-25
  • DOI: 10.7554/elife.44927
  • NAID: 120006888021
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acute nociceptive stimuli rapidly induce the activity of serotonin and noradrenalin neurons in the brain stem of awake mice (2019)
  • Author(s): Moriya Shunpei、Yamashita Akira、Nishi Ryusei、Ikoma Yoko、Yamanaka Akihiro、Kuwaki Tomoyuki
  • Journal Title: IBRO Reports Volume: 7 Pages: 1-9
  • DOI: 10.1016/j.ibror.2019.05.005
  • Peer Reviewed / Open Access
• [Journal Article] CRISPR/Cas9-mediated in vivo gene editing reveals that neuronal 5-HT1A receptors in the dorsal raphe nucleus contribute to body temperature regulation in mice (2019)
  • Author(s): Nishitani Naoya、Ohmura Yu、Nagayasu Kazuki、Shibui Norihiro、Kaneko Shuji、Ohashi Akiko、Yoshida Takayuki、Yamanaka Akihiro、Yoshioka Mitsuhiro
  • Journal Title: Brain Research Volume: 1719 Pages: 243-252
  • DOI: 10.1016/j.brainres.2019.06.009
  • NAID: 120006885155
  • Peer Reviewed
• [Journal Article] Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination (2019)
  • Author(s): Yamanashi T, Maki M, Kojima K, Shibukawa A, Tsukamoto T, Chowdhury S, Yamanaka A, Takagi S, Sudo Y
  • Journal Title: Sci. Rep. Volume: 9(1) Issue: 1 Pages: 7863-7863
  • DOI: 10.1038/s41598-019-44308-x
  • NAID: 120006733902
  • Peer Reviewed / Open Access
• [Journal Article] GABA in the suprachiasmatic nucleus refines circadian output rhythms in mice. (2019)
  • Author(s): Ono D, Honma KI, Yanagawa Y, Yamanaka A, Honma S
  • Journal Title: Commun Biol. Volume: 2 Issue: 1 Pages: 232-232
  • DOI: 10.1038/s42003-019-0483-6
  • Peer Reviewed / Open Access
• [Journal Article] Thin‐fibre receptors expressing acid‐sensing ion channel 3 contribute to muscular mechanical hypersensitivity after exercise (2019)
  • Author(s): Matsubara Takanori、Hayashi Koei、Wakatsuki Koji、Abe Masahiro、Ozaki Noriyuki、Yamanaka Akihiro、Mizumura Kazue、Taguchi Toru
  • Journal Title: European Journal of Pain Volume: 23 Issue: 10 Pages: 1801-1813
  • DOI: 10.1002/ejp.1454
  • Peer Reviewed
• [Journal Article] REM sleep?active MCH neurons are involved in forgetting hippocampus-dependent memories (2019)
  • Author(s): Izawa Shuntaro、Chowdhury Srikanta、Miyazaki Toh、Mukai Yasutaka、Ono Daisuke、Inoue Ryo、Ohmura Yu、Mizoguchi Hiroyuki、Kimura Kazuhiro、Yoshioka Mitsuhiro、Terao Akira、Kilduff Thomas S.、Yamanaka Akihiro
  • Journal Title: Science Volume: 365 Issue: 6459 Pages: 1308-1313
  • DOI: 10.1126/science.aax9238
  • NAID: 120006777541
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transgenic Archaerhodopsin-3 Expression in Hypocretin/Orexin Neurons Engenders Cellular Dysfunction and Features of Type 2 Narcolepsy. (2019)
  • Author(s): Williams RH, Tsunematsu T, Thomas AM, Bogyo K, Yamanaka A*, Kilduff TS*.
  • Journal Title: J Neurosci Volume: 39(47) Issue: 47 Pages: 9435-9452
  • DOI: 10.1523/jneurosci.0311-19.2019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Mechanical allodynia induced by optogenetic sensory nerve excitation activates dopamine signaling and metabolism in medial nucleus accumbens. (2019)
  • Author(s): Sugiyama E, Kondo T, Kuzumaki N, Honda K, Yamanaka A, Narita M, Suematsu M, Sugiura Y.
  • Journal Title: Neurochem Int Volume: 129 Pages: 104494-104494
  • DOI: 10.1016/j.neuint.2019.104494
  • Peer Reviewed / Open Access
• [Journal Article] Glutamatergic neurons in the medial prefrontal cortex mediate the formation and retrieval of cocaine-associated memories in mice (2019)
  • Author(s): Zhang Tong、Yanagida Junko、Kamii Hironori、Wada Shintaro、Domoto Masaki、Sasase Hitoki、Deyama Satoshi、Takarada Takeshi、Hinoi Eiichi、Sakimura Kenji、Yamanaka Akihiro、Maejima Takashi、Mieda Michihiro、Sakurai Takeshi、Nishitani Naoya、Nagayasu Kazuki、Kaneko Shuji、Minami Masabumi、Kaneda Katsuyuki
  • Journal Title: Addiction Biology Volume: 印刷中 Issue: 1 Pages: 1-11
  • DOI: 10.1111/adb.12723
  • Peer Reviewed / Open Access
• [Journal Article] Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo (2019)
  • Author(s): Koizumi K, Inoue M, Chowdhury S, Bito H, Yamanaka A, Ishizuka T, *Yawo H
  • Journal Title: J Physiol Sci Volume: 69(1) Issue: 1 Pages: 65-77
  • DOI: 10.1007/s12576-018-0618-4
  • NAID: 40021804699
  • Peer Reviewed
• [Journal Article] Large Timescale Interrogation of Neuronal Function by Fiberless Optogenetics Using Lanthanide Micro-particles (2019)
  • Author(s): Toh Miyazaki, Srikanta Chowdhury, Takayuki Yamashita, Takanori Matsubara, Hiromu Yawo, Hideya Yuasa and Akihiro Yamanaka
  • Journal Title: Cell Reports Volume: 26 Issue: 4 Pages: 1033-1043
  • DOI: 10.1016/j.celrep.2019.01.001
  • Peer Reviewed / Open Access
• [Journal Article] Preproenkephalin-expressing ventral pallidal neurons control inhibitory avoidance learning. 2019 (in press) (2019)
  • Author(s): Macpherson T, Mizoguchi H, Yamanaka A, Hikida T
  • Journal Title: Neurochem Int Volume: 126 Pages: 11-18
  • DOI: 10.1016/j.neuint.2019.02.011
  • Peer Reviewed / Open Access
• [Journal Article] Upconversion Amplification through Dielectric Superlensing Modulation (2019)
  • Author(s): Liang L, Teh DBL, Dinh ND, Chen W, Chen Q, Wu Y, Chowdhury S, Yamanaka A, Sum TC, Chen CH, Thakor NV, All AH, Liu X
  • Journal Title: Nat Commun Volume: 10(1) Issue: 1 Pages: 1391-1391
  • DOI: 10.1038/s41467-019-09345-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Opposing roles of dopamine receptor D1- and D2-expressing neurons of the anteromedial olfactory tubercle in acquisition of place preference (2019)
  • Author(s): Koshi Murata, Tomoki Kinoshita, Yugo Fukazawa, Kenta Kobayashi, Akihiro Yamanaka, Takatoshi Hikida, Hiroyuki Manabe, and Masahiro Yamaguchi
  • Journal Title: Frontiers in Behavioral Neuroscience Volume: 13 Pages: 50-50
  • DOI: 10.3389/fnbeh.2019.00050
  • Peer Reviewed / Open Access
• [Journal Article] Reward probability and timing uncertainty alter the effect of dorsal raphe serotonin neurons on patience (2018)
  • Author(s): Miyazaki K*, Miyazaki KW*, Yamanaka A, Tokuda T, Tanaka KF, Doya K *These authors contributed equally to this work.
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 2048-2048
  • DOI: 10.1038/s41467-018-04496-y
  • Peer Reviewed / Open Access
• [Journal Article] Partial ablation of the orexin field induces a sub narcoleptic phenotype in a conditional mouse model of orexin neurodegeneration (2018)
  • Author(s): Black SW,* Sun JD, Santiago P, Laihsu A, Kimura N, Yamanaka A, Bersot R, Humphries PS
  • Journal Title: Sleep Volume: 41(9) Issue: 9
  • DOI: 10.1093/sleep/zsy116
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Inactivation of Serotonergic Neurons in the Rostral Medullary Raph? Attenuates Stress-Induced Tachypnea and Tachycardia in Mice (2018)
  • Author(s): Ikoma Yoko、Kusumoto-Yoshida Ikue、Yamanaka Akihiro、Ootsuka Youichirou、Kuwaki Tomoyuki
  • Journal Title: Frontiers in Physiology Volume: 9 Pages: 1-16
  • DOI: 10.3389/fphys.2018.00832
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acute aversive stimuli rapidly increase the activity of ventral tegmental area dopamine neurons in awake mice. (2018)
  • Author(s): *Moriya S, Yamashita A, Kawashima S, Nishi R, Yamanaka A, Kuwaki T
  • Journal Title: Neuroscience Volume: 386 Pages: 16-23
  • DOI: 10.1016/j.neuroscience.2018.06.027
  • Peer Reviewed / Open Access
• [Journal Article] Parallel arousal pathways in the lateral hypothalamus (2018)
  • Author(s): Heiss JE, Yamanaka A, *Kilduff TS
  • Journal Title: eNeuro Volume: 5(4) Issue: 4 Pages: 0228-18
  • DOI: 10.1523/eneuro.0228-18.2018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Calcium Transient Dynamics of Neural Ensembles in the Primary Motor Cortex of Naturally Behaving Monkeys (2018)
  • Author(s): Kondo T, Saito R, Otaka M, Yoshino-Saito K, Yamanaka A, Yamamori T, Watakabe A, Mizukami H, Schnitzer MJ, Tanaka KF, *Ushiba J, *Okano H
  • Journal Title: Cell Rep Volume: 24(8) Issue: 8 Pages: 2191-2195
  • DOI: 10.1016/j.celrep.2018.07.057
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Opiates increase the number of hypocretin-producing cells in human and mouse brain and reverse cataplexy in a mouse model of narcolepsy (2018)
  • Author(s): Thannickal Thomas C.、John Joshi、Shan Ling、Swaab Dick F.、Wu Ming-Fung、Ramanathan Lalini、McGregor Ronald、Chew Keng-Tee、Cornford Marcia、Yamanaka Akihiro、Inutsuka Ayumu、Fronczek Rolf、Lammers Gert Jan、Worley Paul F.、Siegel Jerome M.
  • Journal Title: Science Translational Medicine Volume: 10 Issue: 447 Pages: 447-447
  • DOI: 10.1126/scitranslmed.aao4953
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Neuronal SIRT1 regulates macronutrient-based diet selection through FGF21 and oxytocin signalling in mice (2018)
  • Author(s): Matsui Sho、Sasaki Tsutomu、Kohno Daisuke、Yaku Keisuke、Inutsuka Ayumu、Yokota-Hashimoto Hiromi、Kikuchi Osamu、Suga Takayoshi、Kobayashi Masaki、Yamanaka Akihiro、Harada Akihiro、Nakagawa Takashi、Onaka Tatsushi、Kitamura Tadahiro
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 1-17
  • DOI: 10.1038/s41467-018-07033-z
  • Peer Reviewed / Open Access
• [Presentation] Regulation of non-REM and REM sleep by the hypothalamic neurons. (2022)
  • Author(s): Yamanaka A.
  • Organizer: World Sleep
  • Int'l Joint Research / Invited
• [Presentation] 光による神経活動の操作と記録を用いた睡眠覚醒調節機構の解明. (2022)
  • Author(s): 山中章弘
  • Organizer: ２０２１年度 キャンパスライフ健康支援センター研究推進ＦＤ
  • Invited
• [Presentation] 神経活動の操作と記録によるナルコレプシー症状発現メカニズムの解明. (2022)
  • Author(s): 山中章弘
  • Organizer: Rising Neurologist Club 2022
  • Invited
• [Presentation] 光を用いた視床下部神経活動の記録と操作によって解明する睡眠覚醒調節メカニズム. (2022)
  • Author(s): 山中章弘
  • Organizer: 日本薬学会第142年会
  • Invited
• [Presentation] Identification of neural pathway which converts circadian rhythm to sleep/wakefulness. (2021)
  • Author(s): Yamanaka A.
  • Organizer: 2020 Oriental International Sleep Medicine Summit Forum
  • Int'l Joint Research / Invited
• [Presentation] 視床下部の特定神経細胞の運命操作、活動操作を用いた睡眠覚醒調節機構の解明. (2021)
  • Author(s): 山中章弘
  • Organizer: 第68回日本実験動物学会総会 学術集会委員会シンポジウム
  • Invited
• [Presentation] オレキシン神経活動によるレム睡眠と脱力発作の調節. (2021)
  • Author(s): 山中章弘
  • Organizer: 2021年度生理学研究所研究会 運動・行動から紐解く脳神経回路発達メカニズムの異分野融合研究による解明
  • Invited
• [Presentation] 神経活動操作を用いた睡眠覚醒と記憶の制御機構の解明. (2021)
  • Author(s): 山中章弘
  • Organizer: 第145回日本薬理学会関東部会
  • Invited
• [Presentation] 睡眠覚醒調節におけるオレキシンの役割について. (2021)
  • Author(s): 山中章弘
  • Organizer: 第49回日本頭痛学会総会
  • Invited
• [Presentation] ナルコレプシーモデルマウスを用いた症状発現メカニズムの解明. (2021)
  • Author(s): 山中章弘
  • Organizer: 第30回日本臨床精神神経薬理学会学術集会
  • Invited
• [Presentation] 睡眠、概日リズムの神経制御メカニズム. (2021)
  • Author(s): 山中章弘
  • Organizer: 脳とこころの研究センター ウインタースクール
  • Invited
• [Presentation] 視床下部神経細胞による睡眠覚醒調節機構. (2021)
  • Author(s): 山中章弘
  • Organizer: 第98回日本生理学会大会
  • Invited
• [Presentation] The role of melanin concentrating hormone-producing neurons in the regulation of sleep and memory. (2020)
  • Author(s): Yamanaka A
  • Organizer: 25th Congress of the European Sleep Research Society
  • Int'l Joint Research / Invited
• [Presentation] Regulation of sleep and memory by the hypothalamic neurons. (2020)
  • Author(s): Yamanaka A
  • Organizer: Molecular Cellular Cognition Society (MCCS)
  • Int'l Joint Research / Invited
• [Presentation] Identification of neural pathway which converts circadian rhythm to sleep/wakefulness. (2020)
  • Author(s): Yamanaka A
  • Organizer: 2020 Oriental International Sleep Medicine Summit Forum
  • Int'l Joint Research / Invited
• [Presentation] 睡眠覚醒と記憶の制御メカニズム. (2020)
  • Author(s): 山中章弘
  • Organizer: 第43回日本神経科学大会
  • Invited
• [Presentation] 視床下部神経による睡眠覚醒と記憶の制御. (2020)
  • Author(s): 山中章弘
  • Organizer: 第31回東海睡眠障害研究会
  • Invited
• [Presentation] he suprachiasmatic nucleus regulates wakefulness via CRF neurons in the paraventricular nucleus of the hypothalamus. (2020)
  • Author(s): Ono D, Mukai Y, Hung CJ, Chowdhury S, Sugiyama T, Yamanaka A.
  • Organizer: 第27回日本時間生物学会学術大会
  • Invited
• [Presentation] 睡眠覚醒調節機構とその破綻による睡眠障害のメカニズム. (2020)
  • Author(s): 山中章弘
  • Organizer: 第65回日本口腔外科学会総会・学術大会
  • Invited
• [Presentation] Neural pathway which converts circadian rhythm to sleep/wakefulness. (2020)
  • Author(s): 山中章弘
  • Organizer: 第43回日本分子生物学会年会
  • Invited
• [Presentation] 視床下部神経による睡眠覚醒と記憶の制御 (2020)
  • Author(s): 山中章弘
  • Organizer: 第218回精神科医会及び第174回学術講演会
  • Invited
• [Presentation] オレキシンとは？ 睡眠覚醒調節における役割について (2020)
  • Author(s): 山中章弘
  • Organizer: 東区医師会学術講演会
  • Invited
• [Presentation] Functional interaction between the hypothalamus, septum and hippocampus to regulate sleep and memory (2019)
  • Author(s): Yamanaka A
  • Organizer: NUS Physiology Research Seminar
  • Int'l Joint Research / Invited
• [Presentation] Role of REM sleep on memory (2019)
  • Author(s): Yamanaka A
  • Organizer: Cold Spring Harbor Asia Neurobiology of Behavior & Neuropsychiatric Disorders
  • Int'l Joint Research / Invited
• [Presentation] REM sleep active MCH neurons are involved in forgetting hippocampus-dependent memories (2019)
  • Author(s): Yamanaka A, Izawa S
  • Organizer: IBRO 2019
  • Int'l Joint Research / Invited
• [Presentation] MCH neurons impaired memories during REM sleep (2019)
  • Author(s): Yamanaka A
  • Organizer: 2019 Chinese Sleep Research Society (CSRS) Annual Academic Conference
  • Int'l Joint Research / Invited
• [Presentation] REM sleep active MCH neurons are involved in forgetting hippocampus-dependent memories (2019)
  • Author(s): Yamanaka A
  • Organizer: 6th Congress of Asian College of Neuropsychopharmacology
  • Int'l Joint Research
• [Presentation] 睡眠覚醒調節と記憶の制御に関わる神経の活動記録と操作 (2019)
  • Author(s): 山中章弘
  • Organizer: 平成30年度生命融合科学教育部シンポジウム 神経科学が解き明かす動物の行動：機能の変容と疾患
  • Invited
• [Presentation] 睡眠覚醒調節神経の機能的同定 (2019)
  • Author(s): 山中章弘
  • Organizer: 第92回日本薬理学会年会
  • Invited
• [Presentation] Adeno-associated virus vector micro injection into mice brain to reveal function of neural circuitry involved in the regulation of sleep/wakefulness. (2019)
  • Author(s): Yamanaka A
  • Organizer: FAOPS2019
  • Int'l Joint Research / Invited
• [Presentation] Regulation of sleep and memory (2018)
  • Author(s): Yamanaka A
  • Organizer: 第10回光操作研究会・第2回脳情報動態合同国際シンポジウムJoint symposium of 10th Optogenetics Research Conference and Second International Symposium on Brain Information Dynamics 2018
  • Int'l Joint Research / Invited
• [Presentation] Dissociating orexin-dependent and -independent functions of orexin neurons using novel orexin-Flp knock-in mice (2018)
  • Author(s): Yamanaka A
  • Organizer: 7th International Symposium on Narcolepsy
  • Int'l Joint Research / Invited
• [Presentation] Sleep/wakefulness analysis of orexin and MCH neurons double ablated mice (2018)
  • Author(s): Hung CJ, Ono D, Yamanaka A
  • Organizer: 7th International Symposium on Narcolepsy
  • Int'l Joint Research / Invited
• [Presentation] Orexin and MCH neurons double ablation mice. Isolation of effect of orexin using orexin-Flippase mice. (2018)
  • Author(s): Yamanaka A
  • Organizer: 7th International Symposium on Narcolepsy
  • Int'l Joint Research / Invited
• [Presentation] Neural activity manipulation and behavior control by using fiber-less optogenetics (2018)
  • Author(s): Yamanaka A
  • Organizer: National University of Singapore, Department of Chemistry, Research Seminar
  • Int'l Joint Research / Invited
• [Presentation] Regulatory mechanism of sleep/wakefulness and memory by the hypothalamic neurons (2018)
  • Author(s): 山中章弘
  • Organizer: 島根大学セミナー
  • Invited
• [Presentation] 睡眠覚醒調節、痛み調節におけるオレキシンの役割 (2018)
  • Author(s): 山中章弘
  • Organizer: 第12回日本緩和医療薬学会年会
  • Invited
• [Presentation] 睡眠覚醒調節におけるオレキシンの役割 (2018)
  • Author(s): 山中章弘
  • Organizer: 第41回日本神経科学大会 ランチョンセミナー10
  • Invited
• [Presentation] Role of cholinergic neurons in the septum on the regulation of sleep and memory (2018)
  • Author(s): 山中章弘、宮﨑杜夫
  • Organizer: 第41回日本神経科学大会 シンポジウム： 神経修飾物質による脳状態制御： 先進的実験と計算論的アプローチを結ぶ
  • Invited
• [Presentation] アップコンバージョンを用いたファイバーレス光遺伝学の開発 (2018)
  • Author(s): 宮﨑杜夫、Chowdhury Srikanta、山下貴之、八尾 寛、湯浅英哉、山中章弘
  • Organizer: 第56回日本生物物理学会年会
  • Invited
• [Presentation] Study of regulatory mechanism of sleep/wakefulness and memory by optogenetical neural activity manipulation (2018)
  • Author(s): 山中章弘
  • Organizer: 第91回日本生化学会大会
  • Invited
• [Presentation] 内側中核アセチルコリン作動性神経による睡眠と記憶の制御 (2018)
  • Author(s): 山中章弘
  • Organizer: 記憶研究会2018 記憶・学習の基盤機構と回路研究の新展開へのアプローチ
  • Invited
• [Presentation] 睡眠と記憶を制御する神経活動の操作と記録 (2018)
  • Author(s): 山中章弘
  • Organizer: 第８回生理学研究所・名古屋大学医学系研究科合同シンポジウム
  • Invited
• [Presentation] 睡眠と記憶を制御する神経活動の操作と記録 (2018)
  • Author(s): 山中章弘
  • Organizer: 星薬科大学セミナー
  • Invited
• [Presentation] ファイバーレス光遺伝学を用いた睡眠覚醒と記憶制御のメカニズム解明 (2018)
  • Author(s): 山中章弘
  • Organizer: 第三回ルミノジェネティクス研究会
  • Invited
• [Presentation] 生活習慣病と睡眠覚醒とのただならぬ関係 (2018)
  • Author(s): 山中章弘
  • Organizer: 第3回Cortin Conference
  • Invited
• [Presentation] 腹側被蓋野(VTA)のGABA作動性神経はノンレム睡眠を調節する (2018)
  • Author(s): 山中章弘
  • Organizer: 平成30年度脳研究所共同利用共同研究合同セミナー
  • Invited
• [Book] Optogenetics-Light-Sensing Proteins and Their Applications in Neuroscience and Beyond-Second Edition (2021)
  • Author(s): Chowdhury S, Yamanaka A*
  • Total Pages: 663
  • Publisher: Springer
  • ISBN: 9789811587627
• [Remarks] 研究成果のプレスリリースScience2019
  • URL: http://www.riem.nagoya-u.ac.jp/4/drof1/nr/files/Sci_190920.pdf
• [Remarks] 研究成果のプレスリリースeLife2019a
  • URL: http://www.riem.nagoya-u.ac.jp/4/drof1/nr/kaisetsu/HPVTA-GABA.pdf
• [Remarks] 研究成果のプレスリリースeLife2019b
  • URL: http://www.riem.nagoya-u.ac.jp/4/drof1/nr/kaisetsu/HPOX-FLP.pdf