| Co-Investigator(Kenkyū-buntansha) |
野原 康伸 熊本大学, 大学院先端科学研究部(工), 特任准教授 (30624829)
兒島 憲二 姫路獨協大学, 薬学部, 准教授 (40542759)
横田 文彦 九州大学, アジア・オセアニア研究教育機構, 准教授 (50760451)
吉高 淳夫 北陸先端科学技術大学院大学, 先端科学技術研究科, 准教授 (60263729)
滝田 禎亮 京都大学, 農学研究科, 助教 (70263126)
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| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2019: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The ultimate goal of this research is to establish a novel method to isothermally amplify a target DNA from clinical specimen. Following DNA amplification, micro temperature gradient gel electrophoresis (TGGE) and analysis of DNA pattern were performed using image processing technology to identify mutations. For this purpose, we have carried out the following: 1) Examination of the effects of length and mutational position on the TGGE pattern; 2) Development of a micro TGGE apparatus capable of accommodating 2-5 gels in one electrophoresis cycle; 3) Development of a software to detect inflection points in a TGGE pattern; 4) Alteration of enzymes utilized in an isothermal DNA amplification method, including recombinase polymerase amplification; 5) Construction of networks to obtain clinical specimen in India.
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