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Identification of novel indicator microorganisms for assessing pathogen contamination of groundwater in the Kathmandu Valley

Research Project

Project/Area Number 18KK0297
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 64:Environmental conservation measure and related fields
Research InstitutionUniversity of Yamanashi

Principal Investigator

HARAMOTO Eiji  山梨大学, 大学院総合研究部, 教授 (00401141)

Co-Investigator(Kenkyū-buntansha) 田中 靖浩  山梨大学, 大学院総合研究部, 准教授 (50377587)
清 和成  北里大学, 医療衛生学部, 教授 (80324177)
古川 隼士  北里大学, 医療衛生学部, 講師 (90632729)
Project Period (FY) 2018-10-09 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords健康関連微生物 / 指標微生物 / 地下水 / カトマンズ盆地 / 新型コロナウイルス
Outline of Final Research Achievements

In this study, we investigated the actual situation of contamination of water samples by pathogens, such as viruses and protozoa, and their sources in the Kathmandu Valley, Nepal, where the outbreaks of waterborne diseases caused by pathogens are a serious problem. Based on the analysis of water samples collected during surveys in February-March and August 2019 and July 2020-February 2021, along with previously collected samples, knowledge on the actual prevalence of pathogens in water samples was elucidated. Especially, the successful detection of SARS-CoV-2 RNA in wastewater and river water is a valuable demonstration of the effectiveness of wastewater-based epidemiology, where the overall incidence of the infectious diseases is not yet known.

Academic Significance and Societal Importance of the Research Achievements

病原微生物による水系感染症の発生が深刻な問題となっているネパール・カトマンズ盆地をフィールドに,現地住民の主要な飲料水源である地下水をはじめとする様々な水試料における病原微生物の汚染実態とその汚染源を明らかにした成果は,現地における水の微生物学的安全性を高めていく上で大きく貢献し得るものである。

Report

(5 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (34 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (6 results) Journal Article (11 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 11 results,  Open Access: 8 results) Presentation (17 results) (of which Int'l Joint Research: 13 results,  Invited: 5 results)

  • [Int'l Joint Research] テュレーン大学(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] トリブバン大学(ネパール)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] トリブバン大学(ネパール)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] テュレーン大学(米国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] トリブバン大学(ネパール)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] テュレーン大学(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Detection of SARS-CoV-2 RNA in wastewater, river water, and hospital wastewater of Nepal2022

    • Author(s)
      Tandukar Sarmila、Sthapit Niva、Thakali Ocean、Malla Bikash、Sherchan Samendra P.、Shakya Bijay Man、Shrestha Laxman P.、Sherchand Jeevan B.、Joshi Dev Raj、Lama Bhupendra、Haramoto Eiji
    • Journal Title

      Science of The Total Environment

      Volume: 824 Pages: 153816-153816

    • DOI

      10.1016/j.scitotenv.2022.153816

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Virus reduction at wastewater treatment plants in Nepal2021

    • Author(s)
      Tandukar Sarmila、Ghaju Shrestha Rajani、Malla Bikash、Sthapit Niva、Sherchand Jeevan B.、Sherchan Samendra P.、Haramoto Eiji
    • Journal Title

      Environmental Challenges

      Volume: 5 Pages: 100281-100281

    • DOI

      10.1016/j.envc.2021.100281

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Release of Antibiotic-Resistance Genes from Hospitals and a Wastewater Treatment Plant in the Kathmandu Valley, Nepal2021

    • Author(s)
      Thakali Ocean、Malla Bikash、Tandukar Sarmila、Sthapit Niva、Raya Sunayana、Furukawa Takashi、Sei Kazunari、Sherchand Jeevan B.、Haramoto Eiji
    • Journal Title

      Water

      Volume: 13 Issue: 19 Pages: 2733-2733

    • DOI

      10.3390/w13192733

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Possibility of Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) through Wastewater in Developing Countries2021

    • Author(s)
      Tandukar Sarmila、Khanal Rajendra、Manandhar Rija、Pandey Ashok、Sthapit Niva、Sherchan Samendra P.、Haramoto Eiji
    • Journal Title

      Water

      Volume: 13 Issue: 23 Pages: 3412-3412

    • DOI

      10.3390/w13233412

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Investigation of Shiga Toxin-Producing Escherichia coli in Groundwater, River Water, and Fecal Sources in the Kathmandu Valley, Nepal2020

    • Author(s)
      Sthapit Niva、Malla Bikash、Ghaju Shrestha Rajani、Tandukar Sarmila、Sherchand Jeevan B.、Haramoto Eiji、Kazama Futaba
    • Journal Title

      Water, Air, & Soil Pollution

      Volume: 231 Issue: 12 Pages: 557-557

    • DOI

      10.1007/s11270-020-04920-4

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Host-specific mitochondrial DNA markers for tracking the sources of fecal pollution2020

    • Author(s)
      Malla Bikash、Haramoto Eiji
    • Journal Title

      Current Opinion in Environmental Science & Health

      Volume: 16 Pages: 34-46

    • DOI

      10.1016/j.coesh.2020.02.006

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation of CrAssphage Marker for Tracking Fecal Contamination in River Water in Nepal2020

    • Author(s)
      Ward Lauren M.、Ghaju Shrestha Rajani、Tandukar Sarmila、Sherchand Jeevan B.、Haramoto Eiji、Sherchan Samendra P.
    • Journal Title

      Water, Air, & Soil Pollution

      Volume: 231 Issue: 6 Pages: 282-282

    • DOI

      10.1007/s11270-020-04648-1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Occurrence of Antibiotic Resistance Genes in an Urban River in Nepal2020

    • Author(s)
      Thakali Ocean、Tandukar Sarmila、Brooks John、Sherchan Samendra、Sherchand Jeevan、Haramoto Eiji
    • Journal Title

      Water

      Volume: 12 Issue: 2 Pages: 450-450

    • DOI

      10.3390/w12020450

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Pathogenic Viruses, Pathogen Indicators, and Fecal-Source Markers within Tanker Water and Their Sources in the Kathmandu Valley, Nepal2019

    • Author(s)
      Malla Bikash、Ghaju Shrestha Rajani、Tandukar Sarmila、Bhandari Dinesh、Thakali Ocean、Sherchand Jeevan B.、Haramoto Eiji
    • Journal Title

      Pathogens

      Volume: 8 Issue: 2 Pages: 81-81

    • DOI

      10.3390/pathogens8020081

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of 16S rRNA and Virulence-Associated Genes of Arcobacter in Water Samples in the Kathmandu Valley, Nepal2019

    • Author(s)
      Ghaju Shrestha Rajani、Tanaka Yasuhiro、Sherchand Jeevan B.、Haramoto Eiji
    • Journal Title

      Pathogens

      Volume: 8 Issue: 3 Pages: 110-110

    • DOI

      10.3390/pathogens8030110

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prevalence of Arcobacter and Other Pathogenic Bacteria in River Water in Nepal2019

    • Author(s)
      Shrestha Rajani Ghaju、Tandukar Sarmila、Bhandari Dinesh、Sherchan Samendra P.、Tanaka Yasuhiro、Sherchand Jeevan B.、Haramoto Eiji
    • Journal Title

      Water

      Volume: 11 Issue: 7 Pages: 1416-1416

    • DOI

      10.3390/w11071416

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ネパール・カトマンズ盆地の河川水・生活用水の薬剤耐性遺伝子汚染の実態調査2022

    • Author(s)
      竹澤翼, Mohan Amarasiri, 古川隼士, Bikash Malla, 原本英司, 清和成
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Prevalence of antibiotic resistance genes in drinking and environmental water sources of the Kathmandu Valley, Nepal2022

    • Author(s)
      Tsubasa Takezawa, Mohan Amarasiri, Takashi Furukawa, Bikash Malla, Jeevan B. Sherchand, Eiji Haramoto, Kazunari Sei
    • Organizer
      IWA World Water Congress & Exhibition 2022
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detection of SARS-CoV-2 RNA from two wastewater treatment plants of Nepal2021

    • Author(s)
      Sarmila Tandukar, Ocean Thakali, Niva Sthapit, Bikash Malla, Eiji Haramoto
    • Organizer
      4th International Forum on Asian Water Environment Technology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Wastewater surveillance of enteric viruses for wastewater-based epidemiology2021

    • Author(s)
      Niva Sthapit, Bikash Malla, Sarmila Tandukar, Eiji Haramoto
    • Organizer
      4th International Forum on Asian Water Environment Technology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Health-related water microbiology: wastewater-based epidemiology and microbial source tracking2021

    • Author(s)
      Eiji Haramoto
    • Organizer
      Virtual Conferences: ERIS International Scientific Week
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新型コロナウイルスの下水疫学研究の展開2021

    • Author(s)
      原本英司
    • Organizer
      日本水処理生物学会第57回(神奈川)大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Wastewater surveillance for monitoring of the incidence of COVID-19 in communities2021

    • Author(s)
      Eiji Haramoto
    • Organizer
      STI (Science, Technology and Innovation) for Global Challenges: International Research Collaboration Against the COVID-19 Crisis
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 環境水中における病原微生物の汚染実態と微生物指標の活用2021

    • Author(s)
      原本英司
    • Organizer
      第66回日本水環境学会セミナー「水環境における病原性ウイルスモニタリング技術の動向」
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 水環境中におけるノロウイルスの汚染実態2020

    • Author(s)
      原本英司
    • Organizer
      第69回日本感染症学会東日本地方会学術集会・第67回日本化学療法学会東日本支部総会合同学会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Enteric virus contamination in hospital wastewater of the Kathmandu Valley, Nepal2019

    • Author(s)
      Sarmila Tandukar, Jeevan B. Sherchand, Bikash Malla, Rajani Ghaju Shrestha, Ocean Thakali, Eiji Haramoto
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microbiological situation of environmental water in the Kathmandu Valley, Nepal2019

    • Author(s)
      Ocean Thakali, Bikash Malla, Rajani Ghaju Shrestha, Sarmila Tandukar, Yasuhiro Tanaka, Daisuke Inoue, Kazunari Sei, Jeevan B. Sherchand, Eiji Haramoto
    • Organizer
      7th SURF Workshop on River Basin Environment and Management
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Quantification of Arcobacter and its virulence genes in environmental water samples in the Kathmandu Valley, Nepal2019

    • Author(s)
      Rajani Ghaju Shrestha, Yasuhiro Tanaka, Daisuke Inoue, Kazunari Sei, Jeevan B. Sherchand, Eiji Haramoto
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Performance evaluation of animal-specific viral and mitochondrial DNA markers and their application to microbial source tracking of drinking water sources in the Kathmandu Valley, Nepal2019

    • Author(s)
      Bikash Malla, Jeevan B. Sherchand, Eiji Haramoto
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reduction of enteropathogens at two conventional wastewater treatment plants in Nepal2019

    • Author(s)
      Sarmila Tandukar, Jeevan B. Sherchand, Bikash Malla, Rajani Ghaju Shrestha, Ocean Thakali, Eiji Haramoto
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Abundance and antibiotic resistance of Escherichia coli O157:H7 isolated from hospital wastewater in the Kathmandu Valley, Nepal2019

    • Author(s)
      Ocean Thakali, Sarmila Tandukar, Sunayana Raya, Jeevan B. Sherchand, Takashi Furukawa, Kazunari Sei, Eiji Haramoto
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Investigation of Shiga toxin-producing Escherichia coli in different wastewaters in the Kathmandu Valley, Nepal2019

    • Author(s)
      Niva Sthapit, Bikash Malla, Rajani Ghaju Shrestha, Sarmila Tandukar, Ocean Thakali, Jeevan B. Sherchand, Eiji Haramoto, Futaba Kazama
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Pepper mild mottle virus and crAssphage as fecal pollution markers in aquatic environments of the Kathmandu Valley, Nepal2019

    • Author(s)
      Bikash Malla, Rajani Ghaju Shrestha, Sarmila Tandukar, Jeevan B. Sherchand, Eiji Haramoto
    • Organizer
      20th International Symposium on Health-Related Water Microbiology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2018-10-12   Modified: 2023-01-30  

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