In vivo mass culture system of human iPS cell-derived hepatocytes

• Project/Area Number: 18KK0307
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 90:Biomedical engineering and related fields
• Research Institution: The University of Tokyo (2019-2022); Osaka University (2018)
• Principal Investigator: Mashimo Tomoji 東京大学, 医科学研究所, 教授 (80397554)
• Co-Investigator(Kenkyū-buntansha): 吉見 一人 東京大学, 医科学研究所, 講師 (50709813) ; 武石 一樹 九州大学, 大学病院, 特別教員 (50733713)
• Project Period (FY): 2018-10-09 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2020: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ヒト肝細胞 / iPS細胞 / 免疫不全ラット / ゲノム編集 / 肝移植

Outline of Final Research Achievements

Using the iPS-Heps culture protocol developed by the University of Pittsburgh, we were able to culture a large number of human iPS-Heps cells in the livers of immunodeficient rats through cell transplantation. To recover a significant amount of proliferated iPS-Heps from within the rat liver, we generated immunodeficient rats transgenic with iCasp9 as a suicide gene, but we were unable to confirm cell death in the rat liver. Instead, we successfully produced severe immunodeficient rats with a knock-in of human SIRPA or KIT genes. By inducing the death of hepatocytes in these severe immunodeficient rats, we will establish a method to exclusively generate human iPS-Heps cells in vivo within immunodeficient rats.

Academic Significance and Societal Importance of the Research Achievements

本研究では、Bioreactorとしてラット肝臓内でiPS-Hepsを成熟させ、ヒト肝細胞を大量に作り出す方法を確立する。肝不全に対する革新的新規治療法を開発するだけでなく、創薬でのヒト肝細胞の大量利用を実現する。

Research Products

• [Int'l Joint Research] ピッツバーグ大学(米国)
• [Int'l Joint Research] ピッツバーグ大学(米国)
• [Int'l Joint Research] ピッツバーグ大学(米国)
• [Int'l Joint Research] ピッツバーグ大学(米国)
• [Journal Article] Developmental changes in brain activity of heterozygous Scn1a knockout rats (2023)
  • Author(s): Tahara Mayu、Higurashi Norimichi、Hata Junichi、Nishikawa Masako、Ito Ken、Hirose Shinichi、Kaneko Takehito、Mashimo Tomoji、Sakuma Tetsushi、Yamamoto Takashi、Okano Hirotaka James
  • Journal Title: Frontiers in Neurology Volume: 14 Pages: 1125089-1125089
  • DOI: 10.3389/fneur.2023.1125089
  • Peer Reviewed / Open Access
• [Journal Article] 2. Highly selective transgene expression through the flip-excision switch system by using a unilateral spacer sequence. (2023)
  • Author(s): Matsushita, N.*, Kato, S.*, Nishizawa, K., Sugawara, M., Takeuchi, K., Miyasaka, Y., Mashimo, T. and Kobayashi, K. * Equal contribution
  • Journal Title: Cell Rep. Methods Volume: 3 Issue: 2 Pages: 100393-100393
  • DOI: 10.1016/j.crmeth.2022.100393
  • Peer Reviewed / Open Access
• [Journal Article] A senolytic immunotoxin eliminates p16-positive T cells and ameliorates age-associated phenotypes of CD4+ T cells in a surface marker knock-in mouse (2023)
  • Author(s): Sugiyama Yuma、Harada Tanenobu、Kamei Yuka、Yasuda Tomoharu、Mashimo Tomoji、Nishikimi Akihiko、Maruyama Mitsuo
  • Journal Title: Experimental Gerontology Volume: 174 Pages: 112130-112130
  • DOI: 10.1016/j.exger.2023.112130
  • Peer Reviewed / Open Access
• [Journal Article] BRCA1 haploinsufficiency impairs iron metabolism to promote chrysotile‐induced mesothelioma via ferroptosis resistance (2023)
  • Author(s): Luo Yaguang、Akatsuka Shinya、Motooka Yashiro、Kong Yingyi、Zheng Hao、Mashimo Tomoji、Imaoka Tatsuhiko、Toyokuni Shinya
  • Journal Title: Cancer Science Volume: 114 Issue: 4 Pages: 1423-1436
  • DOI: 10.1111/cas.15705
  • Peer Reviewed / Open Access
• [Journal Article] <i>Cyba</i> and <i>Nox2</i> mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner (2023)
  • Author(s): MORI Masayuki、DAI Jian、MIYAHARA Hiroki、LI Ying、KANG Xiaojing、YOSHIMI Kazuto、MASHIMO Tomoji、HIGUCHI Keiichi、MATSUMOTO Kiyoshi
  • Journal Title: Experimental Animals Volume: 72 Issue: 2 Pages: 233-241
  • DOI: 10.1538/expanim.22-0122
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] The rat Downunder (<i>Du</i>) coat color mutation is associated with eye anomalies and embryonic lethality and maps to a 3.9-Mb region on chromosome 3 (2023)
  • Author(s): Hieu Hoang Trung、Tanaka Miyuu、Kuwamura Mitsuru、Mashimo Tomoji、Serikawa Tadao、Kuramoto Takashi
  • Journal Title: Experimental Animals Volume: 72 Issue: 1 Pages: 88-94
  • DOI: 10.1538/expanim.22-0086
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Thioredoxin deficiency increases oxidative stress and causes bilateral symmetrical degeneration in rat midbrain (2022)
  • Author(s): Ohmori I, Ouchida M, Imai H, Ishida S, Toyokuni S, Mashimo T.
  • Journal Title: Neurobiology of Disease Volume: 175 Pages: 105921-105921
  • DOI: 10.1016/j.nbd.2022.105921
  • Peer Reviewed / Open Access
• [Journal Article] A heterozygous LAMA5 variant may contribute to slowly progressive, vinculin-enhanced familial FSGS and pulmonary defects. (2022)
  • Author(s): Kaimori JY, Kikkawa Y, Motooka D, Namba-Hamano T, Takuwa A, Okazaki A, Kobayashi K, Tanigawa A, Kotani Y, Uno Y, Yoshimi K, Hattori K, Asahina Y, Kajimoto S, Doi Y, Oka T, Sakaguchi Y, Mashimo T, Sekiguchi K, Nakaya A, Nomizu M, Isaka Y.
  • Journal Title: JCI insight Volume: 7 Issue: 23 Pages: 522-524
  • DOI: 10.1172/jci.insight.158378
  • Peer Reviewed / Open Access
• [Journal Article] 3. Catecholaminergic cell type-specific expression of Cre recombinase in knock-in transgenic rats generated by the Combi-CRISPR technology. (2022)
  • Author(s): Matsushita, N., Nishizawa, K., Kato, S., Iguchi, Y., Fukabori, R., Takeuchi, K., Miyasaka, Y., Mashimo, T. and Kobayashi, K.
  • Journal Title: J. Neurosci. Methods Volume: 381 Pages: 109707-109707
  • DOI: 10.1016/j.jneumeth.2022.109707
  • Peer Reviewed / Open Access
• [Journal Article] Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3 (2022)
  • Author(s): Yoshimi Kazuto、Takeshita Kohei、Kodera Noriyuki、Shibumura Satomi、Yamauchi Yuko、Omatsu Mine、Umeda Kenichi、Kunihiro Yayoi、Yamamoto Masaki、Mashimo Tomoji
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 4917-4917
  • DOI: 10.1038/s41467-022-32618-0
  • Peer Reviewed / Open Access
• [Journal Article] A high-quality severe combined immunodeficiency (SCID) rat bioresource. (2022)
  • Author(s): Yoshiki Miyasaka, Jinxi Wang, Kosuke Hattori, Yuko Yamauchi, Miho Hoshi, Kazuto Yoshimi, Saeko Ishida, Tomoji Mashimo,
  • Journal Title: PloS one Volume: 17 Issue: 8 Pages: e0272950-e0272950
  • DOI: 10.1371/journal.pone.0272950
  • Peer Reviewed / Open Access
• [Journal Article] Brca1^L63X/+ rat is a novel model of human BRCA1 deficiency displaying susceptibility to radiation-induced mammary cancer (2022)
  • Author(s): Yuzuki Nakamura, Jo Kubota, Yukiko Nishimura, Kento Nagata, Mayumi Nishimura, Kazuhiro Daino, Atsuko Ishikawa, Takehito Kaneko, Tomoji Mashimo, Toshiaki Kokubo, Masaru Takabatake, Kazumasa Inoue, Masahiro F
  • Journal Title: Cancer science Volume: 113 Issue: 10 Pages: 3362-3375
  • DOI: 10.1111/cas.15485
  • Peer Reviewed / Open Access
• [Journal Article] BRCA1 haploinsufficiency promotes chromosomal amplification under Fenton reaction-based carcinogenesis through ferroptosis-resistance (2022)
  • Author(s): Kong Yingyi、Akatsuka Shinya、Motooka Yashiro、Zheng Hao、Cheng Zhen、Shiraki Yukihiro、Mashimo Tomoji、Imaoka Tatsuhiko、Toyokuni Shinya
  • Journal Title: Redox Biology Volume: 54 Pages: 102356-102356
  • DOI: 10.1016/j.redox.2022.102356
  • Peer Reviewed / Open Access
• [Journal Article] Filaggrin-deficient rats generated using zinc-finger nucleases spontaneously exhibit dry scaly skin (2022)
  • Author(s): Nakashima Chisa、Doi Hiromi、Nakajima Saeko、Mashimo Tomoji、Oga Toru、Ishida-Yamamoto Akemi、Honda Tetsuya、Ishida Yoshihiro、Otsuka Atsushi、Kabashima Kenji
  • Journal Title: Allergology International Volume: 71 Issue: 4 Pages: 545-547
  • DOI: 10.1016/j.alit.2022.04.001
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel animal model of combined generalized and focal epilepsy. (2022)
  • Author(s): Ohmori I, Ouchida M, Shinohara M, Kobayashi K, Ishida S, Mashimo T.
  • Journal Title: Epilepsia Volume: 63 Issue: 7
  • DOI: 10.1111/epi.17295
  • Peer Reviewed / Open Access
• [Journal Article] CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension (2022)
  • Author(s): Kabwe JC, Sawada H, Mitani Y, Oshita H, Tsuboya N, Zhang E, Maruyama J, Miyasaka Y, Ko H, Oya K, Ito H, Yodoya N, Otsuki S, Ohashi H, Okamoto R, Dohi K, Nishimura Y, Mashimo T, Hirayama M, Maruyama K.
  • Journal Title: Respir Res. Volume: ;23 Issue: 1 Pages: 1-22
  • DOI: 10.1186/s12931-022-02005-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Impact of GAD65 and/or GAD67 deficiency on perinatal development in rats. (2022)
  • Author(s): Jiang W, Kakizaki T, Fujihara K, Miyata S, Zhang Y, Suto T, Kato D, Saito S, Shibasaki K, Ishizaki Y, Isoda K, Yokoo H, Obinata H, Hirano T, Miyasaka Y, Mashimo T, Yanagawa Y.
  • Journal Title: .FASEB J . Volume: 36(2) Issue: 2
  • DOI: 10.1096/fj.202101389r
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Study of the CRISPR/Cas3 System for Xenotransplantation (2022)
  • Author(s): Miyagawa S, Watanabe M, Nagashima H, Sato K, Kogata S, Toyama C, Masahata K, Kamiyama M, Yamamoto R, Eguchi H, Maeda A, Yoshimi K, Mashimo T, Okuyama H.
  • Journal Title: Transplant Proc Volume: 54 Issue: 2 Pages: 522-524
  • DOI: 10.1016/j.transproceed.2021.09.070
  • Peer Reviewed
• [Journal Article] A humanised rat model of osteosarcoma reveals ultrastructural differences between bone and mineralised tumour tissue (2022)
  • Author(s): Lahr Christoph A.、Landgraf Marietta、Wagner Ferdinand、Cipitria Amaia、Moreno-Jimenez Ines、Bas Onur、Schmutz Beat、Meinert Christoph、Cavalcanti Amanda Dos Santos、Mashimo Tomoji、Miyasaka Yoshiki、Holzapfel Boris M.、Shafiee Abbas、McGovern Jacqui A.、Hutmacher Dietmar W.
  • Journal Title: Bone Volume: 158 Pages: 116018-116018
  • DOI: 10.1016/j.bone.2021.116018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 受精卵のゲノム編集 (2022)
  • Author(s): 石田紗恵子、真下知士
  • Journal Title: HORMONE FRONTIER IN GYNECOLOGY Volume: Vol.29 No.1 Pages: 31-36
• [Journal Article] Response to correspondence on “Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation” (2021)
  • Author(s): Gurumurthy Channabasavaiah B.、O’Brien Aidan R.、・・・・・・・・・・・・・・・・・・Mashimo Tomoji、・・・・・・・・・・・・・・・Burgio Gaetan
  • Journal Title: Genome Biology Volume: 22 Issue: 1
  • DOI: 10.1186/s13059-021-02320-3
  • Open Access / Int'l Joint Research
• [Journal Article] Genetic Deletion of Glutamate Decarboxylase 67-kDa Isoform Alters Conditioned Fear Behavior in Rats. (2021)
  • Author(s): Fujihara K, Sato T, Miyasaka Y, Mashimo T, Yanagawa Y.
  • Journal Title: FEBS Open Bio. Volume: 11 Issue: 2 Pages: 340-353
  • DOI: 10.1002/2211-5463.13065
  • Peer Reviewed / Open Access
• [Journal Article] PHF24 is expressed in the inhibitory interneurons in rats (2021)
  • Author(s): Numakura Yuki、Uemura Risa、Tanaka Miyuu、Izawa Takeshi、Yamate Jyoji、Kuramoto Takashi、Kaneko Takehito、Mashimo Tomoji、Yamamoto Takashi、Serikawa Tadao、Kuwamura Mitsuru
  • Journal Title: Experimental Animals Volume: 70 Issue: 1 Pages: 137-143
  • DOI: 10.1538/expanim.20-0105
  • NAID: 130007981760
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed
• [Journal Article] CRISPR/Cas9-engineered Gad1 elimination in rats leads to complex behavioral changes: implications for schizophrenia (2020)
  • Author(s): Fujihara Kazuyuki、Yamada Kazuo、Ichitani Yukio、Kakizaki Toshikazu、Jiang Weiru、Miyata Shigeo、Suto Takashi、Kato Daiki、Saito Shigeru、Watanabe Masahiko、Kajita Yuki、Ohshiro Tomokazu、Mushiake Hajime、Miyasaka Yoshiki、Mashimo Tomoji、Yasuda Hiroki、Yanagawa Yuchio
  • Journal Title: Translational Psychiatry Volume: 10 Issue: 1 Pages: 426-426
  • DOI: 10.1038/s41398-020-01108-6
  • Peer Reviewed / Open Access
• [Journal Article] Down syndrome cell adhesion molecule like-1 (DSCAML1) links the GABA system and seizure susceptibility. (2020)
  • Author(s): Hayase Y, Amano S, Hashizume K, Tominaga T, Miyamoto H, Kanno Y, Ueno-Inoue Y, Inoue T, Yamada M, Ogata S, Balan S, Hayashi K, Miura Y, Tokudome K, Ohno Y, Nishijo T, Momiyama T, Yanagawa Y, Takizawa A, Mashimo T, Serikawa T, Sekine A, Nakagawa E, Takeshita E, Yoshikawa T, Waga C, Inoue K, Goto YI, Nabeshima Y, et al.
  • Journal Title: Acta Neuropathol Commun. Volume: 8 Issue: 1 Pages: 206-206
  • DOI: 10.1186/s40478-020-01082-6
  • Peer Reviewed / Open Access
• [Journal Article] Pathophysiological significance of Stim1 mutation in sympathetic response to stress and cardiovascular phenotypes in SHRSP/Izm: In vivo evaluation by creation of a novel gene knock-in rat using CRISPR/Cas9 (2020)
  • Author(s): Odongoo Batbayar、Ohara Hiroki、Ngarashi Davis、Kaneko Takehito、Kunihiro Yayoi、Mashimo Tomoji、Nabika Toru
  • Journal Title: Clinical and Experimental Hypertension Volume: 43 Issue: 1 Pages: 34-41
  • DOI: 10.1080/10641963.2020.1797085
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Generation of knockout rabbits with X-linked severe combined immunodeficiency (X-SCID) using CRISPR/Cas9 (2020)
  • Author(s): Hashikawa Yoshiko、Hayashi Ryuhei、Tajima Masaru、Okubo Toru、Azuma Shohei、Kuwamura Mitsuru、Takai Naofumi、Osada Yasuyuki、Kunihiro Yayoi、Mashimo Tomoji、Nishida Kohji
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 9957-9957
  • DOI: 10.1038/s41598-020-66780-6
  • Peer Reviewed / Open Access
• [Journal Article] Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells (2020)
  • Author(s): Takeishi Kazuki、Collin de l’Hortet Alexandra、Wang Yang、Handa Kan、Mashimo Tomoji、Fox Ira J.、Soto-Gutierrez Alejandro
  • Journal Title: Cell Reports Volume: 31 Issue: 9 Pages: 107711-107711
  • DOI: 10.1016/j.celrep.2020.107711
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ゲノム編集医療－技術開発・治療応用戦略を中心に、特集コーディネーター (2020)
  • Author(s): 真下知士・吉見一人
  • Journal Title: 遺伝子医学 Volume: 第10巻第4号（通巻34号） Pages: 1-176
• [Journal Article] 効率的なゲノム編集ラットの作製方法 (2020)
  • Author(s): 服部晃佑、吉見一人、松下夏樹、小林和人、真下知士
  • Journal Title: マウス・ラットモデル作製・解析プロフェッショナル. あなたの研究をステップアップさせる最新・最適手技. 先端モデル動物支援プラットフォーム（AdAMS） Volume: - Pages: 54-63
• [Journal Article] 高血圧関連疾患モデル動物作製の現状と展望 (2020)
  • Author(s): 山内祐子、真下知士
  • Journal Title: 日本臨牀増刊号「高血圧学（上） Volume: 78巻 Pages: 379-384
• [Journal Article] ゲノム編集技術を用いた遺伝子改変ラットの作製 (2020)
  • Author(s): 宮坂佳樹、真下知士
  • Journal Title: 医学のあゆみ「ゲノム編集の未来」 Volume: Vol.273(9) Pages: 785-794
• [Journal Article] Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair (2020)
  • Author(s): Nakazawa Y, Hara Y, Oka Y, Komine O, van den Heuvel D, Guo C, Daigaku Y, Isono M, He Y, Shimada M, Kato K, Jia N, Hashimoto S, Kotani Y, Miyoshi Y, Tanaka M, Sobue A, Mitsutake N, Suganami T, Masuda A, Ohno K, Nakada S, Mashimo T, Yamanaka K, Luijsterburg MS, Ogi T
  • Journal Title: Cell Volume: 19 Issue: 6 Pages: 1228-1244
  • DOI: 10.1016/j.cell.2020.02.010
  • NAID: 120006871293
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Pathological characteristics of <i>Ccdc85c</i> knockout rats: a rat model of genetic hydrocephalus (2020)
  • Author(s): Konishi S, Tanaka N, Mashimo T, Yamamoto T, Sakuma T, Kaneko T, Tanaka M, Izawa T, Yamate J, Kuwamura M
  • Journal Title: Experimental Animals Volume: 69 Issue: 1 Pages: 26-33
  • DOI: 10.1538/expanim.19-0005
  • NAID: 130007791587
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Endothelial Cell-Selective Adhesion Molecule Contributes to the Development of Definitive Hematopoiesis in the Fetal Liver (2019)
  • Author(s): Ueda Tomoaki、Yokota Takafumi、Okuzaki Daisuke、Uno Yoshihiro、Mashimo Tomoji、Kubota Yoshiaki、Sudo Takao、Ishibashi Tomohiko、Shingai Yasuhiro、Doi Yukiko、Ozawa Takayuki、Nakai Ritsuko、Tanimura Akira、Ichii Michiko、Ezoe Sachiko、Shibayama Hirohiko、Oritani Kenji、Kanakura Yuzuru
  • Journal Title: Stem Cell Reports Volume: 13 Issue: 6 Pages: 992-1005
  • DOI: 10.1016/j.stemcr.2019.11.002
  • Peer Reviewed / Open Access
• [Journal Article] Null mutation of the endothelin receptor type B gene causes embryonic death in the GK rat (2019)
  • Author(s): Wang Jinxi、Dang Ruihua、Miyasaka Yoshiki、Hattori Kousuke、Torigoe Daisuke、Okamura Tadashi、Tag-EI-Din-Hassan Hassan T.、Morimatsu Masami、Mashimo Tomoji、Agui Takashi
  • Journal Title: PLOS ONE Volume: 14 Issue: 6 Pages: e0217132-e0217132
  • DOI: 10.1371/journal.pone.0217132
  • Peer Reviewed
• [Journal Article] 疾患モデルラットの国内外トレンド (2019)
  • Author(s): 真下知士
  • Journal Title: 実験動物技術 Volume: 54 Pages: 91-95
• [Journal Article] ラット受精卵でのゲノム編集 (2019)
  • Author(s): 吉見一人、真下知士
  • Journal Title: 実験医学別冊 ゲノム編集実験スタンダード Volume: -
• [Journal Article] 免疫不全ラットの開発と利用 (2019)
  • Author(s): 15.山内祐子、吉見一人、真下知士
  • Journal Title: LABIO21実験動物としてのラットの有用性 Volume: 78 Pages: 16-19
• [Journal Article] ゲノム編集技術によるモデル動物の作製 (2019)
  • Author(s): 真下 知士
  • Journal Title: メディカル・サイエンス・ダイジェスト Volume: 45 Pages: 17-20
• [Presentation] Genome editing technologies and humanized rats, The 36th International Mammalian Genome Conference (IMGC2023) (2023)
  • Author(s): Tomoji Mashimo
  • Organizer: 第36回国際哺乳類ゲノム会議
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集がひらく明るい未来 (2023)
  • Author(s): 真下知士
  • Organizer: ゲノム編集の未来を考える会 メディアセミナー
  • Invited
• [Presentation] CRISPR-Cas3, a Genome Editing Technology, in Genome Therapy (2023)
  • Author(s): Tomoji Mashimo
  • Organizer: IMSUT NY Seminar 2023
  • Int'l Joint Research / Invited
• [Presentation] ラットにおける遺伝子改変技術とヒト化動物の現状と展望 (2022)
  • Author(s): 真下知士
  • Organizer: 公益社団法事日本実験動物学会主催第10回実験動物科学シンポジウム
  • Invited
• [Presentation] A new genome editing technology CRISPR-Cas3 (2022)
  • Author(s): Tomoji Mashimo
  • Organizer: World Science Leaders' Seminar in Doctoral Program in Human Biology
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集動物作製とCRISPR診断薬CONAN (2022)
  • Author(s): 吉見一人・真下知士
  • Organizer: UTOPIAキックオフミーティング
  • Invited
• [Presentation] 新規ゲノム編集法の開発 (2022)
  • Author(s): 真下知士
  • Organizer: 名古屋大学生命農学研究科大学院セミナー
  • Invited
• [Presentation] A novel genome editing technology (2022)
  • Author(s): Tomoji Mashimo
  • Organizer: CRISPR-Cas3, PASTEUR JAPAN 2022 SYMPOSIUM
  • Int'l Joint Research / Invited
• [Presentation] RISPR-Cas3を中心とした最新のゲノム編集技術と応用 (2022)
  • Author(s): 真下知士
  • Organizer: 富士フィルム株式会社Rゼミ講演
  • Invited
• [Presentation] 遺伝子編集技術 CRISPR-Cas3 の概要と応用事例について (2022)
  • Author(s): 真下知士
  • Organizer: 第40回JBDAバイオベンチャーフォーラム
  • Invited
• [Presentation] 遺伝子編集技術CRISPR-Cas3の概要と応用事例について (2022)
  • Author(s): 真下知士
  • Organizer: 第3回エリクサジェンサイエンティフィックセミナー
  • Invited
• [Presentation] ゲノム編集CRISPR-Cas3の遺伝子治療に向けた研究開発について (2022)
  • Author(s): 真下知士
  • Organizer: ヒトゲノム編集技術のガバナンスと基礎研究・臨床応用に関する委員会（第２５期・第３回）
  • Invited
• [Presentation] ゲノム編集が拓く疾患モデル研究の最先端 (2021)
  • Author(s): 真下知士
  • Organizer: 第68回日本実験動物学会総会
  • Invited
• [Presentation] ゲノム編集技術の最新動向 (2021)
  • Author(s): 真下知士
  • Organizer: 第21回日本抗加齢医学会総会
  • Invited
• [Presentation] 次世代型ゲノム編集技術の開発 (2021)
  • Author(s): 真下知士
  • Organizer: 第73回日本細胞生物学会大会
  • Invited
• [Presentation] ゲノム編集が拓く疾患モデル研究 (2021)
  • Author(s): 真下知士
  • Organizer: 第17 回 北海道実験動物研究会(HALAS)学術集会 特別講演
  • Invited
• [Presentation] 脳神経疾患・モデルの開発 (2021)
  • Author(s): 真下知士
  • Organizer: 日中ハイレベル研究者交流会～パブリックヘルスと生命システム科学～
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集とヒト化動物 (2021)
  • Author(s): 真下知士
  • Organizer: 実験動物に感謝する集い（招待講演）
  • Invited
• [Presentation] ゲノム編集技術の開発や新しいモデル動物の開発動向などについて (2021)
  • Author(s): 真下知士
  • Organizer: 日本実験動物協同組合(JCALA)の研修会
  • Invited
• [Presentation] 遺伝子編集が拓く疾患モデル研究のフロンティア (2020)
  • Author(s): 真下知士
  • Organizer: 第56回高血圧関連疾患モデル学会学術総会、理事長企画／未病栄養シンポジウム
  • Invited
• [Presentation] CRISPR-Cas3がもたらす新たなゲノム編集基盤技術 (2020)
  • Author(s): 真下知士
  • Organizer: 千里ライフサイエンスセミナー「ゲノム編集がもたらす革新と更なる展望」
  • Invited
• [Presentation] CRISPR-Cas3: a new tool for genome editing and nucleic acid detection (2020)
  • Author(s): Tomoji Mashimo
  • Organizer: The 15th International Symposium of the Institute Network for Biomedical Sciences ～Cutting Edge of Biomedical and Metabolic Sciences～
  • Int'l Joint Research / Invited
• [Presentation] New CRISPR Technology for Genome Editing (2020)
  • Author(s): Tomoji Mashimo
  • Organizer: 第84回日本循環器学会学術集会Genome editing: CRISPR, Stem cells, and Beyond
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集技術とその医療応用：基礎技術的視点から (2020)
  • Author(s): 真下知士
  • Organizer: 第2回 Translational and Regulatory Sciences Symposium
  • Invited
• [Presentation] 新規ゲノム編集ツール、遺伝子改変ラット、ヒト化動物の未来 (2019)
  • Author(s): 真下知士
  • Organizer: 第144回関西実験動物研究会
  • Invited
• [Presentation] ゲノム編集による疾患モデルの作製 (2019)
  • Author(s): 真下知士
  • Organizer: 第53回日本実験動物技術者協会総会
  • Invited
• [Presentation] ゲノム編集と重症免疫不全ラットの開発について (2019)
  • Author(s): 真下知士
  • Organizer: 第90回信州実験動物研究会秋期勉強会
  • Invited
• [Presentation] ゲノム編集研究の進展と医工薬への応用 (2019)
  • Author(s): 真下知士
  • Organizer: 関西大学・大阪医科大学・大阪薬科大学医工薬連環科学教育研究機構研究セミナ
  • Invited
• [Presentation] 動物生命科学におけるゲノム編集技術の利用 (2019)
  • Author(s): 真下知士
  • Organizer: TARAセミナー
  • Invited
• [Presentation] RISPR/Cas9-mediated genome editing in zygotes (2019)
  • Author(s): Mashimo T
  • Organizer: The 9th International Forum on Laboratory Animal Science &Technology
  • Int'l Joint Research / Invited
• [Presentation] ラットにおける最先端生殖工学技術 (2019)
  • Author(s): 真下知士
  • Organizer: 第66回日本実験動物学会総会ランチョンセミナー
  • Invited
• [Presentation] CRISPR-mediated genome engineering and its application in animal models (2019)
  • Author(s): Tomoji Mashimo
  • Organizer: 22nd US-Japan Cellular and Gene Therapy Conference, Adeno-associated Virus-Mediated Gene Therapy Gene Therapy
  • Int'l Joint Research / Invited
• [Presentation] トランスレーショナルリサーチにおけるゲノム編集への期待と限界 (2019)
  • Author(s): 真下知士
  • Organizer: 塩見雅志准教授退職記念祝賀会・シンポジウム
  • Invited
• [Presentation] ゲノム編集の最新技術動向 (2018)
  • Author(s): 真下知士
  • Organizer: 第24回国立大学法人動物実験施設協議会高度技術研修会
  • Invited
• [Presentation] マウス・ラットモデル作成の新展開 (2018)
  • Author(s): 真下知士
  • Organizer: 第41回日本分子生物学会年会バイオテクノロジーセミナー
  • Invited
• [Presentation] マウス・ラットにおけるゲノム編集技術の現状 (2018)
  • Author(s): 吉見 一人、真下知士
  • Organizer: 第41回日本分子生物学会年会
• [Book] 腫瘍内科 (2023)
  • Author(s): 藤井智明、真下知士
  • Total Pages: 123
  • Publisher: 科学評論社
• [Book] 真の実臨床応用をめざした再生医療2023 (2023)
  • Author(s): 渡辺祥司，吉見一人，真下知士
  • Total Pages: 226
  • Publisher: 羊土社
  • ISBN: 9784758104081
• [Book] Medical Science Digest 特集 遺伝子治療の最前線 (2022)
  • Author(s): 藤井智明、真下知士
  • Total Pages: 54
  • Publisher: ニューサイエンス社
• [Book] 再生医療 (2022)
  • Author(s): 吉見一人、真下知士
  • Total Pages: 57
  • Publisher: 日本再生医療学会雑誌
• [Remarks] 東京大学医科学研究所実験動物研究施設先進動物ゲノム研究分野
  • URL: https://www.ims.u-tokyo.ac.jp/animal-genetics/index.html
• [Remarks] 東京大学医科学研究所システム疾患モデル研究センター先進モデル動物作製コア
  • URL: https://www.ims.u-tokyo.ac.jp/cemsb/public_html/index.html
• [Remarks] 重症免疫不全（SCID）ラット
  • URL: http://www2.med.osaka-u.ac.jp/gerdc/srpo
• [Remarks] 大阪大学大学院医学系研究科実験動物学講座
  • URL: http://www.iexas-osaka-u.jp/lab/
• [Remarks] 大阪大学大学院医学系研究科附属共同研ゲノム編集センター
  • URL: http://www2.med.osaka-u.ac.jp/gerdc/
• [Remarks] 重症免疫不全（SCID）ラット
  • URL: http://www2.med.osaka-u.ac.jp/gerdc/srpo/