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Concurrent achievement of monoculture and soil biodiversity by grafting

Research Project

Project/Area Number 18KT0040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Agricultural Resources for the Next Generation
Research InstitutionNagoya University

Principal Investigator

Notaguchi Michitaka  名古屋大学, 高等研究院(生), 准教授 (00647927)

Project Period (FY) 2018-07-18 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2020: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2019: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords接木 / トランスクリプトーム解析 / 接木関連遺伝子 / 園芸 / 分子生物学
Outline of Final Research Achievements

Plant grafting is generally performed between closely related species. Recently, we have discovered that Nicotiana species of Solanaceae show the ability to graft with distantly related plant species beyond the family. Graft adhesion with diverse angiosperms by Nicotiana species was probably facilitated by the secretion of a subclade of b-1,4-glucanases. The capability of interfamily grafting was also found in the model Orobanchaceae hemiparasitic plant, Phtheirospermum japonicum, which naturally invades to the tissues of host plants of different families. Transcriptome analysis indicated that the same clade of b-1,4-glucanase plays an important role in plant parasitism. Thus, the tissue adhesion between distant plant species occurs both naturally and artificially.

Academic Significance and Societal Importance of the Research Achievements

異科接木技術に関しては、生物学的な研究によって現象の理解を徹底して進めており、これまでに鍵となる分子の同定にも成功した。科学的な知見を、技術の改善につなげる技術開発では、接着力を高める効果も認められており、今後は実用に向けた技術改良が期待される。接木は、農作物の性能を“直ちに”向上させる技術であり、その組み合わせ次第で現代社会が直面する様々な農業上の課題に対応可能なポテンシャルを持つ。また、植物科学の観点でも、接木は植物免疫や植物の再生といった現象に深く関係しており、本研究で得られたデータから今後の研究で重要な知見が一挙に広がる可能性がある。

Report

(4 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • Research Products

    (33 results)

All 2021 2020 2019 2018 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (25 results) (of which Int'l Joint Research: 9 results,  Invited: 13 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Tissue adhesion between distant plant species in parasitism and grafting2021

    • Author(s)
      Okayasu Koji、Aoki Koh、Kurotani Ken-Ichi、Notaguchi Michitaka
    • Journal Title

      Communicative & Integrative Biology

      Volume: 14 Issue: 1 Pages: 21-23

    • DOI

      10.1080/19420889.2021.1877016

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An in vitro grafting method to quantify mechanical forces of adhering tissues2020

    • Author(s)
      Yaichi Kawakatsu, Yu Sawai, Ken-ichi Kurotani, Katsuhiro Shiratake and Michitaka Notaguchi
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 4 Pages: 451-458

    • DOI

      10.5511/plantbiotechnology.20.0925a

    • NAID

      130007960236

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2020-12-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cell-cell adhesion in plant grafting is facilitated by β-1,4-glucanases2020

    • Author(s)
      Notaguchi, M., Kurotani, K., Sato, Y., Tabata, R., Kawakatsu, Y., Okayasu, K., Sawai, Y., Okada, R., Asahina, M., Ichihashi, Y., Shirasu, K., Suzuki, T., Niwa, M., Higashiyama, T
    • Journal Title

      Science

      Volume: 369 Issue: 6504 Pages: 698-702

    • DOI

      10.1126/science.abc3710

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Host-parasite tissue adhesion by a secreted type of β-1,4-glucanase in the parasitic plant Phtheirospermum japonicum2020

    • Author(s)
      Kurotani, K.-I., Wakatake, T., Ichihashi, Y., Okayasu, K., Sawai, Y., Ogawa, S., Suzuki, T., Shirasu, K., Notaguch, M
    • Journal Title

      bioRxiv.

      Volume: ー Pages: 407-407

    • DOI

      10.1101/2020.03.29.014886

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Micrografting device for testing systemic signaling in Arabidopsis2020

    • Author(s)
      Hiroki Tsutsui, Naoki Yanagisawa, Yaichi Kawakatsu, Shuka Ikematsu, Yu Sawai, Ryo Tabata, Hideyuki Arata, Tetsuya Higashiyama, Michitaka Notaguchi
    • Journal Title

      The Plant Journal

      Volume: 1 Issue: 2 Pages: 1-1

    • DOI

      10.1111/tpj.14768

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] 接ぎ木の科学による展望2021

    • Author(s)
      野田口理孝
    • Organizer
      Science Cafe
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] “接ぎ木”と“寄生”の接着におけるβ-1,4-グルカナーゼ遺伝子についての研究2021

    • Author(s)
      黒谷賢一、田畑 亮、川勝弥一、若竹崇雅、白須賢、野田口 理孝
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Construction of in vitro grafting system of Nicotiana benthamiana2021

    • Author(s)
      Yaichi Kawakatsu, Katsuhiro Shiratake, Michitaka Notaguchi
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Study on mechanism of hetero grafting2020

    • Author(s)
      Michitaka Notaguchi
    • Organizer
      Botany 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 接木において細胞接着を促進するGH9B3遺伝子に関する研究2020

    • Author(s)
      野田口理孝、黒谷賢一、田畑亮、川勝弥一
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 寄生植物Phtheirosper-mum Japonicumと宿主植物の組織癒合ぶあける分泌型β-1.4‐グルカナーゼの機能分析2020

    • Author(s)
      黒谷賢一、若竹 崇雅、市橋 泰範、岡安 浩二、澤井 優、小川 哲史、Songkui Cui、鈴木 孝征、白須 賢、野田口 理孝
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ実生での接木を効率化する接木チップの開発2020

    • Author(s)
      筒井大貴、川勝弥一、柳澤直樹、池松朱夏、澤井優、田畑亮、新田英之、東山哲也、野田口理孝
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] タバコ属のIn vitro graftingシステムの構築2020

    • Author(s)
      川勝弥一、白武勝裕、野田口理孝
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 接木の研究から探る、茎の傷修復機構2020

    • Author(s)
      野田口理孝
    • Organizer
      第6回幹細胞研究会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 接木の科学研究と展望2020

    • Author(s)
      野田口理孝
    • Organizer
      種生物学会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] RNAs as systemic signals in plants and its potential application2019

    • Author(s)
      Michitaka Notaguchi
    • Organizer
      International Symposium on the Future Direction of Plant Science by Young Researchers
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 異科接木の分子機構と技術開発2019

    • Author(s)
      野田口理孝
    • Organizer
      種生物学会シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 接木技術の再考 ストレス土壌の活用を目指して2019

    • Author(s)
      野田口理孝、田畑亮、岡安浩次、澤井優、鈴木孝征、黒谷賢一
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Application for overcoming interfamily grafting and grafting microchip2019

    • Author(s)
      Michitaka Notaguchi
    • Organizer
      International Symposium on Vegetable Grafting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Nicotiana interfamily grafting and grafting microchip2019

    • Author(s)
      Michitaka Notaguchi
    • Organizer
      The 2nd International Symposium on Horticultural Biology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 非自己でもつながる接木法とその未来2019

    • Author(s)
      野田口理孝
    • Organizer
      私立大学戦略的研究基盤形成支援事業プロジェクト・シンポジウム「環境および非自己応答機構から読み解く植物の巧みな生存戦略」
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Development of a micro-grafting chip for Arabidopsis and its application aiming to control nutrition and symbiosis with microbiota2019

    • Author(s)
      Yaichi Kawakatsu, Hiroki Tsutsui, Ryo Tabata, Hirokazu Toju, Michitaka Notaguchi
    • Organizer
      Cold Spring Harbor Asia conference on Plant Cell and Development Biology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study on the mechanism of Nicotiana interfamily grafting2019

    • Author(s)
      Michitaka Notaguchi, Kenichi Kurotani, Ryo Tabata
    • Organizer
      Plant Vascular Biology Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] シロイヌナズナ接ぎ木チップの開発2019

    • Author(s)
      川勝弥一,筒井大貴,柳沢直樹,澤井優,池松朱夏,新田英之,東山哲也,野田口理孝
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] Molecular mechanisms of Nicotiana interfamily grafting2019

    • Author(s)
      Notaguchi M
    • Organizer
      Plant and Animal Genome XXVII Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Advances on plant grafting2019

    • Author(s)
      Notaguchi M
    • Organizer
      5th International Horticulture Research Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Interfamily grafting using a plant genus Nicotiana2019

    • Author(s)
      Notaguchi M, Kurotani K, Okayasu K, Sawai Y, Tsutsui H, Okada R, Niwa M, Sato Y, Higashiyama T
    • Organizer
      International Plant Molecular Biology
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 接ぎ木はいかにして接着するのか? 分子機構の解明と農業への応用に向けた取り組み2019

    • Author(s)
      野田口理孝,岡安浩次,澤井 優,鈴木孝征,黒谷賢一
    • Organizer
      園芸学会平成31年度春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] タバコ属の異科接木における特徴的な遺伝子発現の同定2019

    • Author(s)
      野田口理孝,田畑亮,岡安浩次,澤井優,鈴木孝征,黒谷賢一
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 接ぎ木能力の高いタバコ属植物2018

    • Author(s)
      野田口理孝
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Book] Phloem: Methods and Protocols2019

    • Author(s)
      Liesche, Johannes (Ed.)
    • Total Pages
      473
    • Publisher
      Springer
    • ISBN
      9781493995622
    • Related Report
      2019 Research-status Report
  • [Book] 月刊「アグリバイオ」2018

    • Author(s)
      野田口理孝
    • Total Pages
      5
    • Publisher
      株式会社 北隆館
    • Related Report
      2018 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      https://www.agr.nagoya-u.ac.jp/~hort/notaguchi/

    • Related Report
      2018 Research-status Report

URL: 

Published: 2018-07-20   Modified: 2022-12-28  

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