| Project/Area Number |
19001003
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| Research Category |
Grant-in-Aid for Specially Promoted Research
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
Chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
HIRAMA Masahirohi (2008-2010) 東北大学, 大学院・理研究科, 教授 (10142077)
平間 正博 (2007) Tohoku University, 大学院・理学研究科, 教授 (30165203)
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| Co-Investigator(Kenkyū-buntansha) |
FUJII Ikuo 大阪府立大学, 大学院・理学研究科, 教授 (70189984)
YAMASHITA Syuuji 東北大学, 大学院・理学研究科, 助教 (50419991)
YAMAOKA Kaoru 広島国際大学, 保健医療学部, 教授 (10200586)
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| Co-Investigator(Renkei-kenkyūsha) |
TSUMURAYA Takeshi 大阪府立大学, 大学院・理学研究科, 准教授 (00372855)
SATO Itaru 東北大学, 大学院・理学研究科, 准教授 (80318196)
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| Project Period (FY) |
2007 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥425,100,000 (Direct Cost: ¥327,000,000、Indirect Cost: ¥98,100,000)
Fiscal Year 2010: ¥59,930,000 (Direct Cost: ¥46,100,000、Indirect Cost: ¥13,830,000)
Fiscal Year 2009: ¥110,110,000 (Direct Cost: ¥84,700,000、Indirect Cost: ¥25,410,000)
Fiscal Year 2008: ¥150,930,000 (Direct Cost: ¥116,100,000、Indirect Cost: ¥34,830,000)
Fiscal Year 2007: ¥104,130,000 (Direct Cost: ¥80,100,000、Indirect Cost: ¥24,030,000)
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| Keywords | 全合成 / シガトキシン / モノクローナル抗体 / ELISA / チャネル / エンジイン / C1027 / クロモフォア / Naチャネル / チャネル結合部位 / シガテラ / 抗体 / イムノアッセイ / エンジイン抗生物質 / ナトリウムチャネル / シガトキシンCTX1B / 抗シガトキシン抗体 / ハプテン / ヒト型抗体 / ELISAキット / ケダルシジン / マデュロペプチン / C-1027クロモフォア / サマリウムアイオダイド / カリブ海産シガトキシンC-CTX / 51-HydroxyCTX3C / 還元的脱離反応 / アトロプ異性 |
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| Research Abstract |
We have developed a general method for the total syntheses of ciguatoxins. Practical total synthesis of ciguatoxins allowed us to use them for interdisciplinary researches, such as unveiling the gating mechanism of voltage sensitive sodium channels. Highly sensitive anti-CTXlB monoclonal antibodiy and immunoassay method were developed to detect major congeners of ciguatoxin. Some of the previously prepared antibodies were modified for treating the human ciguatera poisoning. Furthermore, designed pseudo-ciguatoxins were also synthesized as bio-probes for understanding the biological mechanisms. On the other hand, total syntheses of the extremely potent antitumor chromoprotein nine-membered enediyne antibiotics and cortistatins were accomplished.
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