蛋白質相互作用における多重特異性の定量的記述と応用

• Project/Area Number: 19021011
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: The University of Tokyo
• Principal Investigator: 津本 浩平 The University of Tokyo, 大学院・新領域創成科学研究科, 准教授 (90271866)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,800,000 (Direct Cost: ¥4,800,000); Fiscal Year 2008: ¥2,500,000 (Direct Cost: ¥2,500,000); Fiscal Year 2007: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: 蛋白質 / 分子認識 / 生物物理 / 生体分子 / シグナル伝達 / バイオテクノロジー / 生物・生体工学 / 相互作用 / 特異性

Research Abstract

(1)多重特異性相互作用の速度論的・熱力学的解析ならびに結晶構造解析 : 前年度までに確立した発現・調製系ならびに相互作用解析システムを用いて、サイトカイン、IL-15Rα、Tob1と各種相互作用分子との相互作用を、表面プラズモン共鳴法、等温滴定型熱量測定に加えて、TEM、DLS、GPC-MALS等により解析した。これらの解析結果を基に、蛋白質相互作用における、多重特異性を定量的記述により議論することができた。
(2)相互作用を特異的にブロックできる抗体、ペプチドのファージディスプレイを用いた選択とその機能・構造評価 : 前年度取得した抗体の構造・機能評価を進めた。アミロイド線維Aβ1-42について、ファージディスプレイを用いたペプチドライブラリーならびにヒト抗体無作為変異導入ライブラリーから特異的に結合する分子種を取得した。得られた分子の機能・構造評価を行い、線維形成制御と蛋白質高次構造との関連を議論することができた。

Research Products

• [Journal Article] Activation of Leukemia-associated RhoGEF by Galphal3 with significant conformational rearrangements in the interface (2009)
  • Author(s): Nobuchika Suzuki, et.al.
  • Journal Title: J. Biol. Chem (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] Impact of intrinsic cooperative thermodynamics of peptide-MHC corn plexes on antiviral activity of HIV-specific cytotoxic T lymphocytes (2009)
  • Author(s): Chihiro Motozono, et.al.
  • Journal Title: J. Immunol (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] Contributions of interfacial residues of human Interleukin 15 to the specificity and affinity for its private alpha-receptor (2009)
  • Author(s): Sakamoto, et.al.
  • Journal Title: J. Mol. Biol (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] Molecular Glues Carrying Multiple Guanidinium Ion Pendants via an Oligc ether Spacer : Stabilization of Microtubules against Depolymerization (2009)
  • Author(s): Okuro, et.al.
  • Journal Title: J. Amer. Chem. Soc (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] To be excluded or to bind, that is the question : Arginine effects on proteins (2009)
  • Author(s): Nakakido, et.al.
  • Journal Title: Cur. Pharm. Biotechnol (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] Utilization of an arginine-elution method for FLAG-tag chromatography (2009)
  • Author(s): Futatsumori-Sugai, et.al.
  • Journal Title: Protein Expr. Purif (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt concentration (2009)
  • Author(s): Nagatoishi, et.al.
  • Journal Title: Mol. Biosyst (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] Immobilized metal affinity chromatography in the presence of arginine (2009)
  • Author(s): Abe, et.al.
  • Journal Title: Biochem. Biophys. Res. Commun (印刷中)(掲載決定)
  • Peer Reviewed
• [Journal Article] Thermodynamic consequences of mutations in vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody, 528 (2008)
  • Author(s): Makabe, et.al.
  • Journal Title: J Biol Chem 283 Pages: 1156-1166
  • Peer Reviewed
• [Journal Article] Grafting of material-binding function into antibodies : Functionalization by peptide grafting (2008)
  • Author(s): Hattori T, et.al.
  • Journal Title: Biochem Biophys Res Commun 365 Pages: 751-757
  • Peer Reviewed
• [Journal Article] critical contribution of VH-VL interaction to reshaping of en antibody (2008)
  • Author(s): Nakanishi T, et.al.
  • Journal Title: Protein Sci 17 Pages: 261-270
  • Peer Reviewed
• [Journal Article] Critical contribution of aromatic rings to specific recognition of polyether rings (2008)
  • Author(s): Tsumoto K, et.al.
  • Journal Title: J Biol Chem 283 Pages: 12259-12266
  • Peer Reviewed
• [Journal Article] A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus (2008)
  • Author(s): Tanaka Y., et.al.
  • Journal Title: Structure 16 Pages: 488-496
  • Peer Reviewed
• [Journal Article] Thermodynamic analysis reveals that, GTP binding affects the interaction between the alpha-and gamma-subunits of translation initiation factor 2 (2008)
  • Author(s): Nakakido, et.al.
  • Journal Title: Biochem Biophys Res Commun 371 Pages: 596-599
  • Peer Reviewed
• [Journal Article] Effect of an amyloidogenic sequence attached to yellow fluorescent protein (2008)
  • Author(s): Hamada D, et.al.
  • Journal Title: Proteins Struct. Funct. Bioinfor 72 Pages: 811-821
  • Peer Reviewed
• [Journal Article] Expression, Purification, Crystallization, and Primary Diffraction Analysis of CapF, a Capsular Polysaccharide Synthesis Enzyme from Staphylococcus aureus (2008)
  • Author(s): Miyafusa, et.al.
  • Journal Title: Acta Crystallogr. F 64 Pages: 512-515
  • Peer Reviewed
• [Journal Article] How Protein Recognizes Ladder-like Polycyclic Ethers : Interactions between Ciguatoxin (CTX3C) Fragments and Its specific antibody 10C9 (2008)
  • Author(s): Ui, et.al.
  • Journal Title: J. Biol. Chem 283 Pages: 19440-19447
  • Peer Reviewed
• [Journal Article] Electron microscopy and computational studies of Ebh, a giant cell-wall-associated protein from Staphylococcus aureus (2008)
  • Author(s): Sakamoto, et.al.
  • Journal Title: Biochem Biophys Res Commun 376 Pages: 261-266
  • Peer Reviewed
• [Journal Article] Structure-based analysis reveals hydration changes induced by arginine hydrochloride (2008)
  • Author(s): Nakakido, et.al.
  • Journal Title: Biophys. Chem 137 Pages: 105-109
  • Peer Reviewed
• [Journal Article] Structural basis for multimeric heme complexation through a specific protein-heme interaction : the case of the third NEAT domain of IsdH from Staphylococcus aureus (2008)
  • Author(s): Watanabe, et.al.
  • Journal Title: J. Biol. Chem 283 Pages: 28649-59
  • Peer Reviewed
• [Journal Article] Staphylococcus aureus giant protein Ebh is involved in tolerance to transient hyperosmotic pressure (2008)
  • Author(s): Kuroda M, et.al.
  • Journal Title: Biochem. Biophys. Res. Commun 374 Pages: 237-41
  • Peer Reviewed
• [Journal Article] Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera (2008)
  • Author(s): Sogo, et.al.
  • Journal Title: J. Immunol. Methods 337 Pages: 16-23
  • Peer Reviewed
• [Journal Article] Thermodynamic consequences of mutations in vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody, 528. (2008)
  • Author(s): Makabe, et. al.
  • Journal Title: J Biol Chem. 283 Pages: 1156-1166
  • Peer Reviewed
• [Journal Article] Grafting of material-binding function into antibodies Functionalization by peptide grafting. (2008)
  • Author(s): Hattori, et. al.
  • Journal Title: Biochem Biophys Res Commun 365 Pages: 751-757
  • Peer Reviewed
• [Journal Article] Critical contribution of VH-VL interaction to reshaping of an antibody: the case of humanization of anti-lysozyme antibody, HyHEL-10. (2008)
  • Author(s): Nakanishi, et. al.
  • Journal Title: Protein Sci. 17 Pages: 261-270
  • Peer Reviewed
• [Journal Article] Critical contribution of aromatic rings to specific recognition of polyether rings: The case of ciguatoxin CTX3C-ABC and its specific antibody 1C49. (2008)
  • Author(s): Tsumoto, et. al.
  • Journal Title: J Biol Chem. (印刷中)
  • Peer Reviewed
• [Journal Article] A helical string of alternately connected three-helix bundles for the cell wall-associated adhesion protein Ebh from Staphylococcus aureus (2008)
  • Author(s): Tanaka, et. al.
  • Journal Title: Structure 16 Pages: 488-496
  • Peer Reviewed
• [Journal Article] Thermodynamic analysis reveals that GTP binding affects the interaction between the alpha- and gamma-subunits of translation initiation factor 2. (2008)
  • Author(s): Nakakido, et. al.
  • Journal Title: Biochem Biophys Res Commun. (印刷中)
  • Peer Reviewed
• [Journal Article] Effect of an amyloidogenic sequence attached to yellow fluorescent protein. (2008)
  • Author(s): Hamada, et. al.
  • Journal Title: Proteins Struct. Funct. Bioinfor. (印刷中)
  • Peer Reviewed
• [Journal Article] Expression, Purification, Crystallization, and Primary Diffraction Analysis of CapF, a Capsulaz Polysaccharide Synthesis Enzyme from Staphylococcus aureus (2008)
  • Author(s): Miyafusa, et. al.
  • Journal Title: Acta Crystallogr. F (印刷中)
  • Peer Reviewed
• [Journal Article] How Protein Recognizes Ladder-like Polycyclic Ethers: Interactions between Ciguatoxin (CTX3C) Fragments and Its specific antibody 10C9. (2008)
  • Author(s): Ui, et. al.
  • Journal Title: J. Biol. Chem. (印刷中)
  • Peer Reviewed
• [Journal Article] クロマトグラフィーにおける溶媒制御 (2008)
  • Author(s): Arakawa, et. al.
  • Journal Title: 生化学 80 Pages: 45-51
• [Journal Article] アミノ酸を利用したクロマトグラフィー (2008)
  • Author(s): 工藤、津本
  • Journal Title: 月刊バイオテクノロジー (印刷中)
• [Journal Article] Effects of acid exposure on the conformation, stability, and aggregation of monoclonal antibodies. (2007)
  • Author(s): Ejima, et. al.
  • Journal Title: Proteins: Structure, Function, and Bioinformatics 66 Pages: 954-962
  • Peer Reviewed
• [Journal Article] Structural consequences of mutations in interfacial Tyr residues of a protein antigen-antibody complex: The case of HyHEL-10-HEL. (2007)
  • Author(s): Shiroishi, et. al.
  • Journal Title: J.Biol. Chem. 282 Pages: 6783-6791
  • Peer Reviewed
• [Journal Article] A Human Antibody Fragment with High Affinity for Biodegradable Polymer Film. (2007)
  • Author(s): Watanabe, et. al.
  • Journal Title: Bioconjugate Chem. 18 Pages: 645-651
  • Peer Reviewed
• [Journal Article] Improved performance of column chromatography by arginine: Dye-affinity chromatography. (2007)
  • Author(s): Arakawa, et. al.
  • Journal Title: Protein Expr. Purif. 52 Pages: 410-414
  • Peer Reviewed
• [Journal Article] Arginine improves protein elution in hydrophobic interaction chromatography. The cases of human interleukin-6 and activin-A. (2007)
  • Author(s): Tsumoto, et. al.
  • Journal Title: J.Chromatogr. A 1154 Pages: 81-86
  • Peer Reviewed
• [Journal Article] Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions. (2007)
  • Author(s): Nagatoishi, et. al.
  • Journal Title: Biochem. Biophys. Res. Comm. 352 Pages: 812-817
  • Peer Reviewed
• [Journal Article] The N-terminal dormain of elastin-binding protein of Staphylococcus aureus changes its secondary structure in a membrane-mimetic environment. (2007)
  • Author(s): Nagatoishi, et. al.
  • Journal Title: J.Biochem. 142 Pages: 131-134
  • Peer Reviewed
• [Journal Article] Structural and Mutational analyses of Drp35 from Staphylococcus aureus: a possible mechanism for its lactonase activity. (2007)
  • Author(s): Tanaka, et. al.
  • Journal Title: J. Biol. Chem. 282 Pages: 5770-5780
  • Peer Reviewed
• [Journal Article] Suppression of protein interactions by arginine: A proposed mechanism of arginine effects. (2007)
  • Author(s): Arakawa, et. al.
  • Journal Title: Biophys Chem. 127 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Molecular properties of two proteins homologous to PduO-type ATP:cob(I)alamin adenosyltransferase from Sulfolobus tokodaii. (2007)
  • Author(s): Tanaka, et. al.
  • Journal Title: Proteins: Structure, Function, and Bioinformatics 68 Pages: 446-457
  • Peer Reviewed
• [Journal Article] The effects of arginine on protein binding and elution in hydrophobic interaction and ion-exchange chromatography. (2007)
  • Author(s): Watanabe, et. al.
  • Journal Title: Protein Expr. Purif. 54 Pages: 110-116
  • Peer Reviewed
• [Journal Article] Effects of salts on protein-surface interactions: applications for column chromatography. (2007)
  • Author(s): Tsumoto, et. al.
  • Journal Title: J Pharmaceut. Sci. 96 Pages: 1677-1690
  • Peer Reviewed
• [Journal Article] Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography. (2007)
  • Author(s): Arakawa, et. al.
  • Journal Title: J. Biochem. Biophys. Methods 70 Pages: 493-498
  • Peer Reviewed
• [Journal Article] Crystal structure analysis reveals a novel forkhead-associated(FHA) domain of ESAT-6 secretion system C (EssC) protein in Staphylococcus aureus. (2007)
  • Author(s): Tanaka, et. al.
  • Journal Title: Proteins: Structure, Function, and Bioinformatics 69 Pages: 659-664
  • Peer Reviewed
• [Journal Article] Aggregation Analysis of Therapeutic Proteins, Part 2. Analytical Ultracentrifugation and Dynamic Light Scattering. (2007)
  • Author(s): Arakawa, et. al.
  • Journal Title: Biotechnol. International 5(4) Pages: 36-47
  • Peer Reviewed
• [Journal Article] Aggregation Analysis of Therapeutic Proteins, Part 3. Principles and Optimization of Field-Flow Fractionation (FFF). (2007)
  • Author(s): Arakawa, et. al.
  • Journal Title: Biotechnol. International 5(11) Pages: 52-70
  • Peer Reviewed
• [Presentation] 構造・物性解析に基づいた抗体の環状ポリエーテル系毒素ciguatoxin認識機構の解明 (2009)
  • Author(s): 宇井美穂子
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 日本大学船橋キャンパス
• [Presentation] 材料・医療への展開を目指した蛋白質相互作用解析 (2008)
  • Author(s): 津本
  • Organizer: 日本分析化学会東北支部会
  • Place of Presentation: 東北大学
  • Year and Date: 2008-03-22
• [Presentation] IL15受容体α鎖が示す多重特異性に関する速度論解析 (2008)
  • Author(s): 坂本
  • Organizer: 第10回日本化学会生命化学研究会シンポジウム
  • Place of Presentation: 熊本大学
  • Year and Date: 2008-01-11
• [Presentation] 抗体10C9が示すポリエーテル系海洋毒素シガトキシン認識機構 (2008)
  • Author(s): 宇井
  • Organizer: 第10回日本化学会生命化学研究会シンポジウム
  • Place of Presentation: 熊本大学
  • Year and Date: 2008-01-11
• [Presentation] 二本鎖DNA-超好熱菌由来TBP蛋白質間の相互作用が示す熱力学的特性 (2008)
  • Author(s): 長門石
  • Organizer: 第10回日本化学会生命化学研究会シンポジウム
  • Place of Presentation: 熊本大学
  • Year and Date: 2008-01-11
• [Presentation] 「蛋白質相互作用解析:材料・医療への展開」 (2008)
  • Author(s): 津本
  • Organizer: 第10回日本化学会生命化学研究会シンポジウム
  • Place of Presentation: 熊本大学
  • Year and Date: 2008-01-11
• [Presentation] 超好熱菌由来TATA-box結合蛋白質に対するDNAの認識特性 (2008)
  • Author(s): 長門石曉
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] 抗シガトキシン抗体10C9の分子認識機構 (2008)
  • Author(s): 宇井美穂子
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] 翻訳開始因子サブユニット間相互作用の熱力学的解析 (2008)
  • Author(s): 中木戸誠
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] 黄色ブドウ球菌由来Isd蛋白質によるヘム結合能の解析 (2008)
  • Author(s): 渡邊正人
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] アルギニン存在下での金属キレートアフィニティークロマトグラフィー (2008)
  • Author(s): 安部良太
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] ワクチンの創成を目指した黄色ブドウ球菌毒素改変体の分子特性解析 (2008)
  • Author(s): 谷中冴子
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] ポリエーテル系海洋毒素シガトキシン認識抗体10C9の構造学・熱力学的解析 (2008)
  • Author(s): 宇井美穂子
  • Organizer: 第55回毒素シンポジウム-山中湖畔シンポジウム-
  • Place of Presentation: ラフォーレ山中湖
• [Presentation] 好熱性蛋白質に対する温度に対応したDNAの相互作用特性 (2008)
  • Author(s): 長門石曉
  • Organizer: 第3回バイオ関連化学合同シンポジウム
  • Place of Presentation: 東京工業大学
• [Presentation] 抗ciguatoxin抗体とポリエーテル環相互作用解析 (2008)
  • Author(s): 宇井美穂子
  • Organizer: 第3回バイオ関連化学合同シンポジウム2008
  • Place of Presentation: 東京工業大学
• [Presentation] 黄色ブドウ球菌由来Isd蛋白質によるヘム結合能の解析 (2008)
  • Author(s): 渡邊正人
  • Organizer: 第3回バイオ関連化学合同シンポジウム2008
  • Place of Presentation: 東京工業大学
• [Presentation] ワクチンの創成を目指した黄色ブドウ球菌毒素改変体の分子特性解析 (2008)
  • Author(s): 谷中冴子
  • Organizer: 第3回バイオ関連化学合同シンポジウム2008
  • Place of Presentation: 東京工業大学
• [Presentation] 超好熱古細菌由来TATA-box結合蛋白質のSELEX解析 (2008)
  • Author(s): 長門石曉
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] 抗ciguatoxin抗体10C9Fabの抗原認識機構-変異体解析と低分子スクリーン- (2008)
  • Author(s): 宇井美穂子
  • Organizer: 第46回日本生物物理学会年会
  • Place of Presentation: 福岡国際会議場
• [Presentation] IL15とIL15R受容体α鎖の相互作用の精査 (2008)
  • Author(s): 坂本壮
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Structure-based analysis reveals hydration changes induced by Areinine hydrochloride (2008)
  • Author(s): Nakakido Makoto
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Transducer of ErbB2, 1(TOB1)とCNOT7間相互作用の精密解析 (2008)
  • Author(s): 渡邊正人
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 黄色ブドウ球菌由来ヘム鉄取り込み蛋白質におけるヘム鉄受け渡し機構の解析 (2008)
  • Author(s): 安部良太
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 抗アミロイドβ抗体を用いた生体超分子構造形成制御 (2008)
  • Author(s): 有里樹理
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 黄色ブドウ球菌由来莢膜合成蛋白質CapE, CapFの構造・機能解析 (2008)
  • Author(s): 宮房孝光
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] ワクチンの創成を目指した黄色ブドウ球菌毒素改変体の分子特性解析 (2008)
  • Author(s): 谷中冴子
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] タンパク質の構造に与えるアルギニンの効果 (2008)
  • Author(s): 工藤
  • Organizer: 第89回 日本化学会春期年会
  • Place of Presentation: 立教大学
• [Presentation] 黄色ブドウ球菌由来膜表面蛋白質EbpSの構造・物性解析 (2008)
  • Author(s): 中木戸
  • Organizer: 第89回 日本化学会春期年会
  • Place of Presentation: 立教大学
• [Presentation] 構造情報を基盤とした黄色ブドウ球菌由来莢膜合成蛋白質CapFの機能解析 (2008)
  • Author(s): 宮房
  • Organizer: 第89回 日本化学会春期年会
  • Place of Presentation: 立教大学
• [Presentation] 黄色ブドウ球菌由来蛋白質によるヘム受け渡し機構の解析 (2008)
  • Author(s): 安部
  • Organizer: 第89回 日本化学会春期年会
  • Place of Presentation: 立教大学
• [Presentation] ワクチンの創成を目指した黄色ブドウ球菌毒素改変体の分子特性解析 (2008)
  • Author(s): 谷中
  • Organizer: 第89回 日本化学会春期年会
  • Place of Presentation: 立教大学
• [Presentation] さらに進化した蛋白質相互作用の熱力学解析 (2007)
  • Author(s): 津本
  • Organizer: 第45回日本生物物理学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-12-21
• [Presentation] Crystal structure and heme binding property of the third NEAT domain of IsdH from Staphylococcus aureus. (2007)
  • Author(s): Tanaka, et. al.
  • Organizer: The 2nd International Symposium on Diffraction Structure Biology
  • Place of Presentation: 船堀ホール
  • Year and Date: 2007-11-21
• [Presentation] 癌免疫療法の現状と今後 (2007)
  • Author(s): 津本
  • Organizer: 日本癌学会シンポジウム
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2007-10-03
• [Presentation] 不安定な二本鎖DNA条件下における蛋白質・DNA間相互作用の熱力学的解析 (2007)
  • Author(s): 長門石
  • Organizer: 第22回生体機能関連化学シンポジウム若手フォーラム
  • Place of Presentation: 東北大学
  • Year and Date: 2007-09-30
• [Presentation] アルギニンを用いたクロマトグラフィー:抗体を中心に (2007)
  • Author(s): 津本
  • Organizer: 第204回液体クロマトグラフィー研究懇談会
  • Place of Presentation: 東京理科大学
  • Year and Date: 2007-06-29
• [Presentation] Role of Arginine in Handling Proteins. (2007)
  • Author(s): Tsumoto
  • Organizer: 第1回日本スイスケミカルバイオロジー会議
  • Place of Presentation: スイスETH(ローザンヌ)
  • Year and Date: 2007-06-25
• [Presentation] Thermodynamic analysis of the interaction between a hyperthermophilic TATA-BOX binding protein (TBP) and the promoter DNA at high temperature (2007)
  • Author(s): Nagatoishi, et. al.
  • Organizer: Current Trends in Microcalorimetry Conference
  • Place of Presentation: ボストンコルネドホテル
• [Presentation] Thermodynamic profiles of the interaction between DNA and DNA-binding protein at high temperature; the case of hyperthermostable TATA-box binding protein and the promoter DNA (2007)
  • Author(s): Nagatoishi, et. al.
  • Organizer: The 2nd Iternational Workshop on Approaches to Single-Cell Analysis
  • Place of Presentation: 早稲田大学
• [Presentation] X線結晶構造解析による抗シガトキシン抗体の分子認識機構の解明 (2007)
  • Author(s): 宇井
  • Organizer: 第10回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 早稲田大学
• [Presentation] 超好熱古細菌由来TATA-box結合蛋白質のDNA結合における熱力学的特性 (2007)
  • Author(s): 長門石
  • Organizer: 第10回バイオテクノロジー部会シンポジウム
  • Place of Presentation: 早稲田大学
• [Presentation] 抗シガトキシン抗体の分子認識機構:結晶構造と熱力学 (2007)
  • Author(s): 宇井
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
• [Presentation] 黄色ブドウ球菌由来莢膜合成蛋白質CapFのX線結晶構造解析 (2007)
  • Author(s): 宮房
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
• [Presentation] アルギニンの示す蛋白質凝集抑制効果:作用機序と応用 (2007)
  • Author(s): 田中
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
• [Presentation] 黄色ブドウ球菌由来接着関連蛋白質EbpSの構造・物性解析 (2007)
  • Author(s): 中木戸
  • Organizer: 第22回生体機能関連化学シンポジウム
  • Place of Presentation: 東北大学
• [Presentation] IL15受容体α鎖が示す多重特異性に関する相互作用解析 (2007)
  • Author(s): 坂本
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 相互作用解析を主としたTransducer of erbB2-1(TOB1)の機能解析 (2007)
  • Author(s): 渡邊
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 黄色ブドウ球菌由来莢膜合成蛋白質の構造機能解析 (2007)
  • Author(s): 宮房
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 抗シガトキシン抗体10C9 Fabの構造学的・熱力学的解析 (2007)
  • Author(s): 宇井
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 高温領域における超好熱古細菌由来TATA-box binding proteinの二本鎖DNA認識機構 (2007)
  • Author(s): 長門石
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 翻訳開始因子サブユニットの会合の熱力学的解析 (2007)
  • Author(s): 中木戸
  • Organizer: 日本生物物理学会第45回年会
  • Place of Presentation: パシフィコ横浜
• [Book] たんぱく質結晶の新展開 (2008)
  • Author(s): 津本、田中
  • Publisher: シーエムシー出版(印刷中)
• [Remarks]
  • URL: http://www.park.itc.u?tokyo/phys?biochem/