Subcellular dissection and dynamic analysis of intracellular metabolites using non-linear photonics

• Project/Area Number: 19021030
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Kinki University (2008); Osaka University (2007)
• Principal Investigator: KAJIYAMA Shinichiro Kinki University, 生物理工学部, 准教授 (20243496)
• Co-Investigator(Kenkyū-buntansha): YANAGIHARA Itaru 大阪府母子センター, 研究所, 研究員 (60314415)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥5,000,000 (Direct Cost: ¥5,000,000); Fiscal Year 2008: ¥2,500,000 (Direct Cost: ¥2,500,000); Fiscal Year 2007: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: レーザ細胞加工 / サブセルラーマイクロサンプリング / 非線形光学顕微鏡 / 細胞内凝集性タンパク質 / 細胞凝集性タンパク質 / 分子間相互作用 / 可視化 / 毒性発現機構 / 相互作用 / アミロイド / 凝集タンパク質 / 転写産物分析

Research Abstract

本研究課題では,極短パルスレーザーによる非線形光学効果を利用して,新しい細胞内代謝産物の取得方法と解析方法を開発するとともに,これらを用いて細胞毒性を有する細菌由来アミロイド様凝集性タンパク質の細胞内での作用機作の解析を行った.この過程で,フェムト秒レーザーを用いた細胞微細加工装置により細胞内に凝集するタンパク質部分を切削・回収することに成功するとともに,非線形光学顕微鏡による無線色での細胞の観察に成功した.

Research Products

• [Journal Article] Akio Kobayashi (2009) High-resolution spatial and temporal analysis of phytoalexin production in oats (2009)
  • Author(s): Yoshihiro Izumi, Shin'ichiro Kajiyama,Ryosuke Nakamura, Atsushi Ishihara, Atsushi Okazawa, Eiichiro Fukusaki, Yasuo Kanematsu
  • Journal Title: Planta 229 Pages: 931-943
• [Journal Article] Line-scanning microscopy for time-gated and spectrally-resolved fluorescence imaging (2008)
  • Author(s): Nakamura, R., Izumi, Y., Kajiyama, S., Kobayashi, A., Kanematsu, Y
  • Journal Title: J. Biol. Phys 34 Pages: 51-62
• [Journal Article] Transient Gene Expression in Guard Cell Chloroplasts of Tobacco Using ArF Excimer Laser Microablation (2008)
  • Author(s): Kajiyama, S., Joseph, B., Inoue, F.,Shimamura, M., Fukusaki, E., Tomizawa, K., Kobayashi, A
  • Journal Title: Journal of Bioscience and Bioengineering 106 Pages: 194-198
• [Journal Article] Molecular basis of action reorganization promoted by binding of enterohaemorrhagic Escherichia coli EspB to alpha-catenin (2008)
  • Author(s): Hamaguchi M, Hamada D, Suzuki KN, Sakata I, Yanagihara I
  • Journal Title: FEBS J 275 Pages: 6260-6267
• [Journal Article] Effect of an amyloidogenic sequence attached to yellow fluorescent protein (2008)
  • Author(s): Hamada D, Tsumoto K, Sawara M, Tanaka N, Nakahira K, Shiraki K, Yanagihara I
  • Journal Title: Proteins 72 Pages: 811-821
• [Journal Article] Transient Gene Expression in Guard Cell Chloroplasts of Tobacco Using ArF Excimer Laser Microablation (2008)
  • Author(s): Kajiyama, S., et.al.
  • Journal Title: J. Biosci. Bioeng. 106(2) Pages: 194-198
  • Peer Reviewed
• [Journal Article] Line-scanning microscopy for time-gated and spectrally-resolved fluorescence imaging (2008)
  • Author(s): Nakamura, R., et.al.
  • Journal Title: J. Biol. Phys. 34 Pages: 51-62
  • Peer Reviewed
• [Journal Article] Molecular basis of action reorganization promoted by binding of enter ohaemorrhagic Escherichia coli EspB to alpha-catenin (2008)
  • Author(s): Hamaguchi M., et.al.
  • Journal Title: FEBS J 275(24) Pages: 6260-6267
  • Peer Reviewed
• [Journal Article] Effect of an amyloidogenic sequence attached to yellow fluorescent protein (2008)
  • Author(s): Hamada D., et.al.
  • Journal Title: Proteins 72(3) Pages: 811-821
  • Peer Reviewed
• [Journal Article] Improvement of transformation efficiecy in bioactive beads-mediated gene transfer using DNA-Iipofectin complex as entrapped genetic material (2008)
  • Author(s): Murakawa, T.・Kajiyama, S.・Ikeuchi, T.・Kawakami, S.・Fukui, K.
  • Journal Title: Journal of Bioscience and Bioengineering (印刷中)
  • Peer Reviewed
• [Journal Article] Femtosecond Pulsed Laser as a Micro-Scalpel for Microdissection and Isolation of Specific Sections from Biological Samples (2007)
  • Author(s): Sakakura M, Kajiyama S, Tsutsumi M, Si J, Fukusaki E, Tamaru Y, Akiyama S, Miura K, Hirao K, and Ueda M
  • Journal Title: Japanese Journal of Applied Physics 46 Pages: 5859-5864
  • NAID: 40015602604
• [Journal Article] Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser (2007)
  • Author(s): S., Kajiyama・F., Inoue・Y., Yoshikawa・T., Shoji・E., Fukusaki・A., Kobayashi
  • Journal Title: Plant Biotechnology 24 Pages: 315-320
  • Peer Reviewed
• [Journal Article] Femtosecond Pulsed Laser as a Micro-Scalpel for Microdissection and Isolation of Specific Sections from Biological Samples (2007)
  • Author(s): Sakakura, M.・Kajiyama, S.・Tsutsumi, M.・Si, J.・Fukusaki, E.・Tamaru, Y.・Akiyama, S.・Miura, K.・Hirao, K.・Ueda, M.
  • Journal Title: Japanese Journal of Applied Physics 46 Pages: 5859-5864
  • NAID: 40015602604
  • Peer Reviewed
• [Presentation] Stimulated parametric emission microscopy for high-resolution 3-D imaging of unstained live-cell (2008)
  • Author(s): Dang, H. et.al.
  • Organizer: 3次元画像コンファレンス2008
  • Place of Presentation: 東京大学
  • Year and Date: 2008-07-11
• [Presentation] Stimulated parametric emission microscopy for high-resolution 3-D imaging of unstained live-cell (2008)
  • Author(s): Dang, H., Kawasumi, T., Kajiyama, S., Ozeki, Y., Itoh, K., Fukui, K
  • Organizer: 3次元画像コンファレンス
  • Place of Presentation: 東京大学
• [Presentation] Single cell sampling and analysis byultra short pulse laser (2007)
  • Author(s): Kajiyama, S・Sakakura, M・Ueda, M・Itoh, K・Fukui, K.
  • Organizer: 5th International forum of post genome technologies
  • Place of Presentation: Bamboo Grove Hotel, Suzhou, China
  • Year and Date: 2007-09-11
• [Presentation] Single cell sampling and analysis by ultra short pulse laser (2007)
  • Author(s): Kajiyama S, Sakakura M, Ueda M, Itoh K, and Fukui K
  • Organizer: 5th International forum of post genome technologies
  • Place of Presentation: Bamboo Grove Hotel, Suzhou, China. (Invited)
• [Book] 「ナノイメージング」(執筆分担)第4編第3節 (2008)
  • Author(s): 梶山慎一郎
  • Publisher: 株式会社NTS
• [Book] ナノイメージング (2008)
  • Author(s): 梶山慎一朗(分担執筆)
  • Publisher: NTS
• [Book] 「ナノバイオ大辞典」(執筆分担山根恒夫、松永是、民谷栄一監修) (2007)
  • Author(s): 梶山慎一郎
  • Publisher: 株式会社テクノシステム
• [Patent(Industrial Property Rights)] PCRにより増幅された伸長DNA鎖の検出方法、および、標的配列の測定方法 (2006)
  • Inventor(s): 梶山慎一郎,福崎英一郎,梨原善行,広実慶彦
  • Industrial Property Rights Holder: 大阪大学
  • Industrial Property Number: 2006-346582
  • Filing Date: 2006-12-22