Malaria parasite pattern recognition mechanism of Anopheline mosquito

• Project/Area Number: 19041007
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: FUKUMOTO Shinya Obihiro University of Agriculture and Veterinary Medicine, 原虫病研究センター, 講師 (50376422)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥8,500,000 (Direct Cost: ¥8,500,000); Fiscal Year 2008: ¥4,300,000 (Direct Cost: ¥4,300,000); Fiscal Year 2007: ¥4,200,000 (Direct Cost: ¥4,200,000)
• Keywords: マラリア / ハマダラカ / Furrowed / ショウジョウバエ

Research Abstract

ショウジョウバエを蚊の代替生物として用いることにより、蚊の体内における新規のマラリア原虫認識分子Furrowed の同定を行い、ハマダラカ中腸におけるパターン認識分子としてマラリア原虫排除因子として機能していることを明らかにした。また、吸血時に、腸内細菌数の一過性の急激な増加が起こることが明らかになり、これはFurrowed の発現上昇パターンと高い相関性を示し、ハマダラカにおけるマラリア原虫の排除には腸内細菌が関与している可能性が示唆された。

Research Products

• [Journal Article] Rapid recruitment of innate immunity regulates variation of intracellular pathogen resistance (2009)
  • Author(s): Okado, K., Shinzawa, N., Aonuma, H., Nelson, B., Fukumoto, S., Fujisaki, K., Kawazu, S., Kanuka, H.
  • Journal Title: Drosophila 379 Pages: 6-10
  • Peer Reviewed
• [Journal Article] Rapid identification of virus-carrying mosquitoes using reverse transcription-loop-mediated isothermal amplification (2009)
  • Author(s): Perera, N., Aonuma, H., Yoshimura, A., Teramoto, T., Iseki, H., Nelson, B., Igarashi, I., Yagi, T., Fukumoto, S., Kanuka, H.
  • Journal Title: J Virol Methods. 156 Pages: 32-6
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model. (2009)
  • Author(s): Aonuma, H., Yoshimura, A., Perera, N., Shinzawa, N., Bando, H., Oshiro, S., Nelson, B., Fukumoto, S., Kanuka, H.
  • Journal Title: Parasit Vectors 2 Pages: 15-15
  • Peer Reviewed
• [Journal Article] Rapid identification of Plasmodiumcarrying mosquitoes using loop-mediated isothermal amplification (2009)
  • Author(s): Aonuma, H. Suzuki, M. Iseki, H. Perera, N. Nelson, B. Igarashi, I. Yagi, T. Kanuka, H. Fukumoto, S.
  • Journal Title: Biochem Biophys Res Commun 376 Pages: 671-676
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors : Dirofilaria immitis as a study model (2009)
  • Author(s): Aonuma, H. Yoshimura, A. Perera, N. Shinzawa, N. Bando, H. Oshiro, S. Nelson, B. Fukumoto. S. Kanuka, H.
  • Journal Title: Parasit Vectors 2 Pages: 15-21
  • Peer Reviewed
• [Journal Article] Rapid recruitment of innate immunity regulates variation of intrac ellular pathogen resistance in Drosophila (2009)
  • Author(s): Okado, K. Shinzawa, N. Aonuma, H. Nelson, B. Fukumoto, S. Fujisaki, K. Kawazu, S. Kanuka, H.
  • Journal Title: Biochem Biophys Res Commun 379 Pages: 6-10
  • Peer Reviewed
• [Journal Article] Rapid identification of viruscarrying mosquitoes using reverse transcription-loopmediated isothermal amplification (2009)
  • Author(s): Perera, N. Aonuma, H. Yoshimura, A. Teramoto, T. Iseki, H. Nelson, B. Igarashi, I. Yagi, T. Fukumoto, S. Kanuka, H.
  • Journal Title: J Virol Methods 156 Pages: 32-36
  • Peer Reviewed
• [Journal Article] Rapid Identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification (2008)
  • Author(s): Aonuma, H., Suzuki, M., Iseki, H., Perera, N., Nelson, B., Igarashi, I., Yagi, T., Kanuka, H., Fukumoto, S.
  • Journal Title: Biochem Biophys Res Commun. 376 Pages: 671-6
  • Peer Reviewed
• [Presentation] 等温遺伝子増幅法(LAMP法)の蚊媒介性感染症への応用 (2009)
  • Author(s): 青沼宏佳、鈴木萌美、吉村文、井関博、土井裕子、五十嵐郁男、平田晴之、嘉糠洋陸、福本晋也
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎
  • Year and Date: 2009-09-25
• [Presentation] 新規アリールアミジン系化合物T-2307の抗マラリア活性 (2009)
  • Author(s): 木村亜希子、福本晋也、野村伸彦、満山順一、井上昇、河津信一郎
  • Organizer: 第78回日本寄生虫学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] Invitro and In vivo Antimalarial activity of T-2037, a novel Arylamidine (2009)
  • Author(s): 木村亜希子、福本晋也、野村伸彦、満山順一、井上昇、河津信一郎、五十嵐郁男
  • Organizer: 第42回日米医学協力・寄生虫疾患部会合同会議
  • Place of Presentation: 東京
  • Year and Date: 2009-01-07
• [Presentation] マラリア原虫における温度ロバストネス機構 (2008)
  • Author(s): 土井裕子、福本晋也、小林朋美、岡野栄之、嘉糠洋陸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 兵庫
  • Year and Date: 2008-12-10
• [Presentation] 貪食細胞による囲い込みは細菌感染に対するtolerance 機能を宿主に付与する (2008)
  • Author(s): 新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 兵庫
  • Year and Date: 2008-12-10
• [Presentation] 蚊・マラリア原虫・腸内細菌の三者の相互作用 (2008)
  • Author(s): 伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 兵庫
  • Year and Date: 2008-12-10
• [Presentation] 感染症媒介蚊・腸内細菌・病原体の間に存在するインターフェース (2008)
  • Author(s): 伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 第8回昆虫病理研究会シンポジウム
  • Place of Presentation: 山梨
  • Year and Date: 2008-09-11
• [Presentation] Impact of midgut bacteria on Plasmodium development in mosquito (2008)
  • Author(s): 伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 8th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫
  • Year and Date: 2008-09-10
• [Presentation] Cellular encapsulation-based host tolerance in bacterial infection (2008)
  • Author(s): 新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
  • Organizer: 8th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫
  • Year and Date: 2008-09-10
• [Presentation] Impact of midgut bacteria on Plasmodium development in mosqulto (2008)
  • Author(s): 伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 8th Awaji International Forum of Infbction and Immunity
  • Place of Presentation: 淡路
  • Year and Date: 2008-09-10
• [Presentation] 細菌感染における感受性の多様性と認識シグナル伝達メカニズム (2008)
  • Author(s): 岡戸清、新澤直明、青沼宏佳、福本晋也、河津信一郎、嘉糠洋陸
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2008-05-26
• [Presentation] マラリア媒介蚊における腸内細菌と病原体のインターフェース (2008)
  • Author(s): 伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2008-05-26
• [Presentation] ウイルス媒介蚊における抗ウイルス反応メカニズムの解析 (2008)
  • Author(s): 寺本時靖、吉村文、福本晋也、嘉糠洋陸
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2008-05-26
• [Presentation] 細菌感染におけるトレランス機能による新規感染防御メカニズム (2008)
  • Author(s): 新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2008-05-26
• [Presentation] 等温遺伝子増幅法(LAMP法)のマラリア原虫媒介蚊への応用 (2008)
  • Author(s): 青沼宏佳、鈴木萌美、井関博、土井裕子、五十嵐郁男、嘉糠洋陸、福本晋也
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2008-05-26
• [Presentation] ロバストネスによるマラリア原虫温度適応メカニズム (2008)
  • Author(s): 土井裕子、福本晋也、岡野栄之、嘉糠洋陸
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2008-05-26
• [Presentation] マラリア媒介蚊における腸内細菌と病原体のインターフェース (2008)
  • Author(s): 伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 第77回日本寄生虫学会
  • Place of Presentation: 札幌
  • Year and Date: 2008-05-26
• [Presentation] ハマダラカ中腸に存在する細菌がマラリア原虫の発育に与える影響 (2008)
  • Author(s): 伴戸寛徳, 青沼宏佳, 福本晋也, 嘉糠洋陸
  • Organizer: 第77回日本寄生虫学会
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-02
• [Presentation] ハマダラカ中腸に存在する細菌がマラリア原虫の発育に与える影響 (2008)
  • Author(s): 伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: 日本分子生物学会第8回春季シンポジウム
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-02
• [Presentation] Why can't the insect vector be killed by an arbovirus? (2007)
  • Author(s): 寺本時靖、宮内亜宜、吉村文、福本晋也、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection andn Immunity
  • Place of Presentation: 兵庫
  • Year and Date: 2007-09-02
• [Presentation] Pattern recognition of Plasmodium parasite in mosquito (2007)
  • Author(s): 福本晋也、青沼宏佳、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection andn Immunity
  • Place of Presentation: 兵庫
  • Year and Date: 2007-09-02
• [Presentation] Interaction between vectors and parasites: Filaria behavior at blood-feeding of mosquito (2007)
  • Author(s): 吉村文、福本晋也、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-09-02
• [Presentation] Why can't the insect vector be killed by an arbovirus? (2007)
  • Author(s): 寺本時靖、宮内亜宜、吉村文、福本晋也、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-09-02
• [Presentation] Pattern recognition of Plasmodium parasite in mosquito (2007)
  • Author(s): 福本晋也、青沼宏佳、嘉糠洋陸
  • Organizer: 7th Awaji International Forum of Infection and Immunity
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-09-02
• [Presentation] David Schneider Furrowed/CTLSE1 aborts development of Plasmodium parasite in Anopheles gambiae EMBO WORKSHOP "Molecular and Population Biology of Mosquitoes and Other Disease (2007)
  • Author(s): 青沼宏佳、Stephanie Brandt、福本晋也、寺本時靖、三浦正幸、八木健、嘉糠洋陸
  • Organizer: Vectors
  • Place of Presentation: ギリシャ
  • Year and Date: 2007-07-14
• [Presentation] Furrowed/CTLSEI aborts development of Plasmodium parasite in Anopheles gambiae (2007)
  • Author(s): 青沼宏佳、Stephanie Brandt、福本晋也、寺本時靖、三浦正幸、八木健、嘉糠洋陵、David Schneider
  • Organizer: EMBO WORKSHOP "Molecular and Population Biology of Mosquitoes and Other Disease Vectors
  • Place of Presentation: ギリシャ
  • Year and Date: 2007-07-14
• [Presentation] Revisiting CO2-activated mosquito behavior (2007)
  • Author(s): 前川絵美、福本晋也、徳永史生、嘉糠洋陸
  • Organizer: EMBO WORKSHOP "Molecular and Population Biology of Mosquitoes and Other Disease Vectors
  • Place of Presentation: ギリシャ
  • Year and Date: 2007-07-14
• [Presentation] What Drosophila Can Tell Us About Insect-borne Diseases: Mosquito Immune Mechanisms Against Malaria Parasite (2007)
  • Author(s): 青沼宏佳、福本晋也、寺本時靖、三浦正幸、八木健、David Schneider、嘉糠洋陸
  • Organizer: ショウジョウバエ研究会第8回研究集会
  • Place of Presentation: 兵庫
  • Year and Date: 2007-07-03
• [Presentation] Fly Immunity: Drosophila Host Resistance Against Bacterial Infection (2007)
  • Author(s): 新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
  • Organizer: ショウジョウバエ研究会第8回研究集会
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-07-03
• [Presentation] Fly Immunity: Diversity of Drosophila Susceptibility to Bacterial Infection (2007)
  • Author(s): 岡戸清、新澤直明、青沼宏佳、福本晋也、嘉糠洋陸
  • Organizer: ショウジョウバエ研究会第8回研究集会
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-07-03
• [Presentation] What Drosophila Can Tell Us About Insect-borne Diseases: Mosquito Immune Mechanisms Against Malaria Parasite (2007)
  • Author(s): 青沼宏佳、福本晋也、寺本時靖、三浦正幸、八木健、David Schneider、嘉糠洋陸
  • Organizer: ショウジョウバエ研究会第8回研究集会
  • Place of Presentation: 兵庫県
  • Year and Date: 2007-07-03