Persistent infection of Hepatitis C virus to get over the acquired immunity system

• Project/Area Number: 19041077
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Metropolitan Organization for Medical Research
• Principal Investigator: KOHARA Michinori Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 参事研究員 (10250218)
• Co-Investigator(Kenkyū-buntansha): YASUI Fumihiko 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 研究員 (40399473) ; HAYASHI Masahiro 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 研究員 (70446566) ; NURIYA Hideko 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 主任研究員 (80270685) ; YONEKAWA Hiromichi 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 参事研究員 (30142110) ; SEKIGUCHI Satoshi 財団法人東京都医学研究機構, 東京都臨床医学総合研究所, 研究員 (10462780)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥16,400,000 (Direct Cost: ¥16,400,000); Fiscal Year 2008: ¥8,300,000 (Direct Cost: ¥8,300,000); Fiscal Year 2007: ¥8,100,000 (Direct Cost: ¥8,100,000)
• Keywords: C型肝炎ウイルス / 持続感染 / 自然免疫 / 獲得免疫 / トランスジェニックマウス

Research Abstract

HCV感染の大きな特徴として感染後高率に持続感染化し、慢性肝炎、さらに肝細胞癌を発症することがあげられる。HCVの持続感染および免疫系の撹乱機序などを明らかにし、ウイルス感染に対する新たな防御法および治療法を確立することを目的とする。HCVの病原性発現機序を解析するためにHCV遺伝子導入トランスジェニックマウスの開発を行い、任意の時期にHCV遺伝子をスイッチング発現するトランスジェニック(Cre/loxP/HCV-MxCre Tg)マウスを樹立した。これにより、正常な免疫応答下で持続的にHCV蛋白を発現させることを可能にした。Cre/loxP/HCV-MxCre Tgマウスは正常な免疫応答下で持続的にHCV蛋白を発現させることができ、慢性肝炎を発症させることができるHCV持続感染モデルとして非常に有用であり、これまで困難とされていたHCVの持続感染化と宿主の免疫応答の関係を解析することを可能にした。

Research Products

• [Journal Article] Disruption of IFN Signaling and HCV Synergistically Enhance Lymphoproliferation through Type II CD95 and Interleukins (2009)
  • Author(s): Keigo Machida, Kyoko Tsukiyama-Kohara,Eiji Seike, ShigenobuTone, Yukiko Hayashi, Yuri Kasama, Masumi Shimizu, Hidemi Takahashi, Chyoji Taya, Hiromichi Yonekawa, Nobuyuki Tanaka, and Michinori Kohara
  • Journal Title: Gastroenterology
• [Journal Article] Synthesis of nuclease-resistant siRNAs possessing universal overhangs (2009)
  • Author(s): Yoshihito Ueno, Yuuji Watanabe, Aya Shibata, Kayo Yoshikawa, Takashi Takano, Michinori Kohara, Yukio Kitade
  • Journal Title: Bioorganic & Medicinal Chemistry 17(5) Pages: 1974-81
  • Peer Reviewed
• [Journal Article] Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain (2009)
  • Author(s): Leiyun Weng, Jiamu Du, Jingling Zhou, Jianping Ding, Takaji Wakita, Michinori Kohara, Tetsuya Toyoda
  • Journal Title: Arch Virol. 154(5) Pages: 765-773
  • Peer Reviewed
• [Journal Article] Synthesis of nuclease-resistant siRNAs possessing universal overhangs. (2009)
  • Author(s): Ueno Y.
  • Journal Title: Bioorganic & Medicinal Chemistry (in press)
  • Peer Reviewed
• [Journal Article] Disruption of IFN signaling and HCV synergistically enhance lymphoproliferation through type II CD95 and interleukins. (2009)
  • Author(s): Machida K.
  • Journal Title: Gastroenterology (in press)
  • Peer Reviewed
• [Journal Article] Modification of hepatitis C virus 1b RNA polymerase to make a highly active JFH1-type polymerase by mutation of the thumb domain. (2009)
  • Author(s): Leiyun Weng
  • Journal Title: Arch. Virol. (in press)
  • Peer Reviewed
• [Journal Article] Hepatitis C virus NS5A protein interacts with and negatively regulates the non-receptor protein-tyrosine kinase Syk (2008)
  • Author(s): Sachiko Inubushi, Motoko Nagano-Fujii, Kikumi Kitayama, Motofumi Tanaka, Chunying An, Hiroshi Yokozaki, Hirohei Yamamura, Hideko Nuriya, Michinori Kohara, Kiyano Sada, and Hak Hotta
  • Journal Title: J. Gen. Virology 89 Pages: 1231-1242
  • Peer Reviewed
• [Journal Article] Comparative aspects on the role of polypyrimidine tract-binding proteinin internal initiation of hepatitis C virus and picornavirus RNAs (2008)
  • Author(s): T. Nishimura, M. Saito, T. Takano, A. Nomoto, M. Kohara, K. Tsukiyama-Kohara
  • Journal Title: Compara. Immu. Microbio. Infect. Dises 31 Pages: 435-448
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virus infection and expression in vitro and in vivo by recombinant adenovirus expressing short hairpin RNA. J (2008)
  • Author(s): Naoya Sakamoto, Yoko Tanabe, Takanori Yokota, Kenichi Satoh, Yuko Sekine-Osajima, Mina Nakagawa, Yasuhiro Itsui, Megumi Tasaka, Yuki Sakurai, Chen Cheng-Hsin, Masahiko Yano, Shogo Ohkoshi, Yutaka Aoyagi, Shinya Maekawa, Nobuyuki Enomoto, Michinori Kohara, and Mamoru Watanabe
  • Journal Title: Gastroenterology Hepatology 23(9) Pages: 1437-47
  • Peer Reviewed
• [Journal Article] Prior immunization with SARS-CoV nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV (2008)
  • Author(s): Fumihiko Yasui, Chieko Kai, Masahiro Kitabatake, Shingo Inoue, Misako Yoneda, Shoji Yokochi, Ryoichi Kase, Satoshi Sekiguchi, Kouichi Morita, Tsunekazu Hishima, Hidenori Suzuki, Katsuo Karamatsu, Yasuhiro Yasutomi, Hisatoshi Shida, Minoru Kidokoro, Kyosuke Mizuno, Kouji Matsushima, Michinori Kohara
  • Journal Title: J. Immunology 181(9) Pages: 6337-48
  • Peer Reviewed
• [Journal Article] A mutational shift from domain III to II in the internal ribosome entry site of hepatitis C virus after interferon-ribavirin therapy (2008)
  • Author(s): Kei Ogata, Takahito Kashiwagi, Jun Iwahashi, Koyu Hara, Haruhito Honda, Tatsuya Ide, Ryukichi Kumashiro, Michinori Kohara, Michio Sata, and Nobuyuki Hamada
  • Journal Title: Arch Virol 153(8) Pages: 1575-9
  • Peer Reviewed
• [Journal Article] C型肝炎ウイルス;宿主因子を標的にしたHCV増殖抑制・阻害を目指して (2008)
  • Author(s): 小原道法
  • Journal Title: ファルマシア 44(1) Pages: 17-21
• [Journal Article] 宿主因子を標的としたC型肝炎ウイルスの抑制 (2008)
  • Author(s): 平田雄一、須藤正幸、小原道法
  • Journal Title: ウイルス 58(2) Pages: 207-214
  • NAID: 10023888598
• [Journal Article] Hepatitis C virus NS5A protein interacts with and negatively regulates the non-receptor protein-tyrosine kinase Syk. (2008)
  • Author(s): Inubishi S.
  • Journal Title: J. Gen. Virology 89 Pages: 1231-1242
  • Peer Reviewed
• [Journal Article] Comparative aspects on the role of polypyrimidine tract-binding protein in internal initiation of hepatitis C virus and picornavirus RNAs. (2008)
  • Author(s): Nishimura T.
  • Journal Title: Compara. Immu. Microbio. Infect. Dises. 31 Pages: 435-448
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virus infection and expression in vitro and in vivo by recombinant ade ovirus expressing short hairpin RNA. (2008)
  • Author(s): Sakamoto N.
  • Journal Title: J. Gastroenterology 23(9) Pages: 1437-47
  • Peer Reviewed
• [Journal Article] Prior immunization with SARS-CoV nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV. (2008)
  • Author(s): Yasui F.
  • Journal Title: J. Immunology 181(9) Pages: 6337-48
  • Peer Reviewed
• [Journal Article] A mutational shift from domain III to II in the internal ribosome entry site of hepatitis C virus after interferon-ribavirin therapy. (2008)
  • Author(s): Ogata K.
  • Journal Title: Arch. Virol. 153(8) Pages: 1575-9
  • Peer Reviewed
• [Journal Article] Comparative aspects on the role of polypyrimidine tract-binding protein in internal initiation of hepatitis C virus and picornavirs RNAs. (2008)
  • Author(s): Nishimura T
  • Journal Title: Compara.Immu.Microbio.Infect.Dises. (in press)
  • Peer Reviewed
• [Journal Article] SARS-CoV spike protein recombinant vaccinia virus efficiently induces neutralizing antibodies in spite of pre-immunization with vaccinia virus (2007)
  • Author(s): Masahiro Kitabatake, Shingo Inoue, Fumihiko Yasui, Shoji Yokochi, Masaaki Arai, Kouichi Morita, Hisatoshi Shida, Minoru Kidokoro, Fukashi Murai, Mai Quynh Le, Kouji Matsushima and Michinori Kohara
  • Journal Title: Vaccine 25 Pages: 630-637
  • Peer Reviewed
• [Journal Article] Inhibition of Hsp90 suppresses HCV replication in replicon cell lines and in chimeric mice with humanized liver (2007)
  • Author(s): Shin-ichiro Nakagawa, Takuya Umehara, Chiho Matsuda, Shusuke Kuge, Masayuki Sudoh and Michinori Kohara.
  • Journal Title: Biochem. Biophys. Res. Commun 353 Pages: 882-888
  • Peer Reviewed
• [Journal Article] Evaluation of a Cyclophilin Inhibitor in Hepatitis C Virus-Infected Chimeric Mice In Vivo (2007)
  • Author(s): Kazuaki Inoue, Takuya Umehara, Urs T. Ruegg, Fumihiko Yasui, Tsunamasa Watanabe, Hiroshi Yasuda, Makoto Yoshiba, Jean-Maurice Dumont, Pietro Scalfaro, Michinori Kohara
  • Journal Title: Hepatology 45 Pages: 921-928
  • Peer Reviewed
• [Journal Article] Identification of CTL epitopes in hepatitis C virus by a genome-wide computational scanning and a rational design of peptide vaccine (2007)
  • Author(s): Toshie Mashiba, Keiko Udaka, Yasuko Hirachi, Yoichi Hiasa, Tomoya Miyakawa, Yoko Satta, Tsutomu Osoda, Sayo Kataoka, Michinori Kohara and Morikazu Onji
  • Journal Title: Immunogenetics 59 Pages: 197-209
  • Peer Reviewed
• [Journal Article] Survival of liver failure pigs by transplantation of reversibly immortalized human hepatocytes with Tamoxifen-mediated self-recombination (2007)
  • Author(s): Totsugawa T, Yong C, Rivas-Carrillo JD, Soto-Gutierrez A, Navarro-Alvarez N, Noguchi H, Okitsu T, Westerman KA, Kohara M, Reth M, Tanaka N, Leboulch P, Kobayashi N
  • Journal Title: J. Hepatology 47 Pages: 74-82
  • Peer Reviewed
• [Journal Article] Liver target delivery of small interfering RNA to the HCV gene by lactosylated cationic liposome (2007)
  • Author(s): Tsunamasa Watanabe, Takuya Umehara, Fumihiko Yasui, Shin-ichiro Nakagawa, Junichi Yano, Tadaaki Ohgi, Satoru Sonoke, Kazuaki Inoue, Makoto Yoshiba and Michinori Kohara
  • Journal Title: J. Hepatology 47 Pages: 744-750
  • Peer Reviewed
• [Journal Article] Therapeutic application of RNA interference for Hepatitis C Virus (2007)
  • Author(s): Tsunamasa Watanabe, Takuya Umehara, Michinori Kohara
  • Journal Title: Advanced Drug Delivery Reviews 59 Pages: 1263-1276
  • Peer Reviewed
• [Journal Article] スフィンゴ脂質生成とC型肝炎ウイルス増殖 (2007)
  • Author(s): 楳原琢哉、坂本洋、須藤正幸、小原道法
  • Journal Title: BIO Clinica 22(1) Pages: 90-95
• [Journal Article] SARS-CoV spike protein recombinant vaccinia virus efficiently induces neutralizing antibodies in spite of pre-immunization with vaccinia virus (2007)
  • Author(s): Kitabatake M
  • Journal Title: Vaccine 25 Pages: 630-637
  • Peer Reviewed
• [Journal Article] Inhibition of Hsp90 suppresses HCV replication in replicon cell lines and in chimeric mice with humanized liver. (2007)
  • Author(s): Nakagawa S
  • Journal Title: Biochem.Biophys.Res.Commun. 353 Pages: 882-888
  • Peer Reviewed
• [Journal Article] Evaluation of the cyclophilin inhibitor DEBIO-025 in hepatitis C virus-infected chimeric mice in vivo. (2007)
  • Author(s): Inoue K
  • Journal Title: Hepatology 45 Pages: 921-928
  • Peer Reviewed
• [Journal Article] Identification of CTL epitopes in hepatitis C virus by a genome-wide computational scanning and a rational design of peptide vaccine. (2007)
  • Author(s): Mashiba T
  • Journal Title: Immunogenetics 59 Pages: 197-209
  • Peer Reviewed
• [Journal Article] Survival of liver failure pigs by transplantation of reversibly immortaliz-ed human hepatocytes with Tamoxifen-mediated self-recombination (2007)
  • Author(s): Totsugawa T
  • Journal Title: J.Hepatology 47 Pages: 74-82
  • Peer Reviewed
• [Journal Article] Liver target delivery of small interferg RNA to the HCV gene by Lactosylated cationic liposome. (2007)
  • Author(s): Watanabe T
  • Journal Title: J.Hepatology 47 Pages: 744-750
  • Peer Reviewed
• [Presentation] SPT阻害剤によるC型肝炎ウイルス(HCV)複製阻害機序の解析 (2009)
  • Author(s): 小原道法
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-28
• [Presentation] スフィンゴミエリンはC型肝炎ウイルス複製に必要な脂質成分である (2008)
  • Author(s): 平田雄一
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] HCV遺伝子組換えワクチニアウイルスの治療ワクチン効果 (2008)
  • Author(s): 関口敏
  • Organizer: 第12回日本ワクチン学会学術総会
  • Place of Presentation: 熊本
  • Year and Date: 2008-11-09
• [Presentation] HCV遺伝子組換えワクチニアウイルスの作製とワクチンとしての検討 (2008)
  • Author(s): 千代智子
  • Organizer: 第12回日本ワクチン学会学術総会
  • Place of Presentation: 熊本
  • Year and Date: 2008-11-09
• [Presentation] Characterization of HCV replication by DHCR24. (2008)
  • Author(s): Takano T.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-10-30
• [Presentation] Regulation of HCV replication by DHCR24. (2008)
  • Author(s): Kohara M
  • Organizer: 15^<th> International Symposium on hepatitis C virus & related viruses.
  • Place of Presentation: Texas
  • Year and Date: 2008-10-07
• [Presentation] Presistent infection of hepatitis C virus to get over the acquired immunity system (2008)
  • Author(s): Sekiguchi S.
  • Organizer: 第8回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 兵庫
  • Year and Date: 2008-09-09
• [Presentation] Serine palmitoyltransferase inhibitor suppresses HCV replication in a mouse model. (2008)
  • Author(s): Kohara M.
  • Organizer: XIV International Congress of Virology
  • Place of Presentation: Istanbul
  • Year and Date: 2008-08-15
• [Presentation] Serine palmitoyltransferase inhibitor suppresses HCV replication in a mouse model. (2008)
  • Author(s): Kohara M.
  • Organizer: 21^<th> International Conference on Antiviral Research
  • Place of Presentation: Montreal
  • Year and Date: 2008-04-14
• [Presentation] Serine Palmitoyltransferase Inhibitor Suppresses HCV Replication in a Mouse Model (2008)
  • Author(s): Hirata Y. Umehara T., Sudoh M., Yasui F., Kohara M
  • Organizer: 21th International Conference on Antiviral Research
  • Place of Presentation: Montreal
• [Presentation] Prior immunization with SARS-CoVnucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV (2008)
  • Author(s): Yasui F., Kai C., Kohara M.
  • Organizer: XIV International Congress of Virology
  • Place of Presentation: Istanbul
• [Presentation] Sudoh M., Kohara M.: Serine palmitoyltransferase inhibitor suppresses HCV replication in a mouse model (2008)
  • Author(s): Hirata Y., Umehara T
  • Organizer: XIV International Congress of Virology
  • Place of Presentation: Istanbul
• [Presentation] Presistent infection of Hepatitis C virus to get over the acquired immunity system (2008)
  • Author(s): Sekiguchi S., Tobita Y., Kohara M.
  • Organizer: 第8回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 兵庫県
• [Presentation] Regulation of HCV replication by DHCR24 (2008)
  • Author(s): Takano T., Hirata Y., Tsukiyama-Kohara K., Sudoh M., Kohara M
  • Organizer: 15th International Symposium on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Texas
• [Presentation] Proteome analysis of HCV inhibitor from natural product, onjisaponin (2008)
  • Author(s): Sudoh M., Itoh T., Aoki M., Ohmori Y., Hirata Y., Yasui F., Kohara M., Aoki Y.
  • Organizer: 15th International Symposium on Hepatitis C Virus & Related Viruses
  • Place of Presentation: Texas
• [Presentation] 新規HCV持続感染モデルマウスの作製とその病態解析 (2008)
  • Author(s): 関口 敏、飛田良美、千代智子、小原道法
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] DHCR24によるHCV複製能制御の検討 (2008)
  • Author(s): 小原道法、高野貴士、平田雄一、小原恭子
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] SPT阻害剤によるHCV複製阻害機序の解析 (2008)
  • Author(s): 平田雄一、須藤正幸、池田和貴、楳原琢哉、飛田良美、田口 良、小原道法
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
• [Presentation] Characterization of HCV replication by DHCR24 (2008)
  • Author(s): Takano T., Hirata Y., Tukiyama-Kohara K., Kohara M.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] :HCV遺伝子組換えワクチニアウイルスの治療ワクチン効果 (2008)
  • Author(s): 関口 敏、千代智子、飛田良美、松原明弘、保富康宏、水野喬介、村井 深、小原道法
  • Organizer: 第12回日本ワクチン学会学術集会
  • Place of Presentation: 熊本
• [Presentation] HCV遺伝子組換えワクチニアウイルスの作製とワクチンとしての検討 (2008)
  • Author(s): 千代智子、関口 敏、松原明弘、保富康宏、脇田隆字、志田壽利、水野喬介、村井 深、小原道法
  • Organizer: 第12回日本ワクチン学会学術集会
  • Place of Presentation: 熊本
• [Presentation] 高RNAi活性をもつDicer認識型siRNA配列の同定 (2008)
  • Author(s): 松田千穂、渡邊綱正、須藤正幸、楳原琢哉、小原道法
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] HCV遺伝子スイッチング発現Tgマウスにおける改良型Cre組換えアデノウイルスの効果 (2008)
  • Author(s): 千代智子、関口 敏、林 昌弘、小原道法
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] スフィンゴミエリンは、C型肝炎ウイルス(HCV)複製に必要な脂質成分である (2008)
  • Author(s): 平田雄一、池田和貴、須藤正幸、楳原琢哉、飛田良美、田口 良、小原道法
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] 宿主因子を標的にしたC型肝炎ウイルス複製の制御 (2007)
  • Author(s): 小原 道法
  • Organizer: 第21回臨床肝臓カンファレンス
  • Place of Presentation: 東京
  • Year and Date: 2007-06-30
• [Presentation] スフィンゴ脂質とC型肝炎ウイルス複製 (2007)
  • Author(s): 小原道法
  • Organizer: 第49回日本脂質生化学会
  • Place of Presentation: 札幌
• [Presentation] Persistent infection of Hepatitis C Virus (2007)
  • Author(s): Kohara M., Sekiguchi S., Tobita Y
  • Organizer: 第7回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 兵庫
• [Presentation] Inter-genotype comparison of replicating and infecting abilities using partitioned-full genomic replicon system (2007)
  • Author(s): Arai M., Suzuki H., Tobita Y., Okamoto K., Ohta A., Sudoh M., Kohara M.
  • Organizer: 14th International Symposium on Hepatitis C Virus & Related Viruses
  • Place of Presentation: GLASGOW
• [Presentation] Hsp90 inhibitors suppress HCV replication in replicon cells and humanized-liver mice (2007)
  • Author(s): Nakagawa S., Kohara M.
  • Organizer: 14th International Symposium on Hepatitis C Virus & Related Viruses
  • Place of Presentation: GLASGOW
• [Presentation] The cyclophillin inhibitor DEBIO-025 shows anti-HCV effect in chimeric mouse with human hepatocytes (2007)
  • Author(s): Inoue K., Umehara T., Watanabe T., Yoshiba M., Kohara M.
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 免疫抑制作用のないサイクロスポリンA誘導体によるヒト肝臓キメラマウスにおけるHCV複製阻害 (2007)
  • Author(s): 井上和明、楳原琢哉、安井文彦、渡邊綱正、与芝真彰、小原道法
  • Organizer: 第55回日本ウイルス学会学術集会
  • Place of Presentation: 札幌
• [Presentation] SRAS-CoVヌクレオキャプシドタンパク質の免疫によるSARS-CoV感染後の肺炎重篤化への関与 (2007)
  • Author(s): 安井文彦、甲斐知恵子、北畠正大、井上真吾、米田美佐子、森田公一、松島綱治、小原道法
  • Organizer: SRAS-CoVヌクレオキャプシドタンパク質の免疫によるSARS-CoV感染後の肺炎重篤化への関与
  • Place of Presentation: 札幌
• [Presentation] Immunizationof SARS-CoV nucleocapsid protein is involved in an aggravation of pulmonary inflammatory response to subsequent SARS-CoV infection (2007)
  • Author(s): Yasui F., Kai C., Kitabatake M., Inoue S., Morita K. Matsushima K., Kohara M
  • Organizer: 第37回日本免疫学会
  • Place of Presentation: 東京
• [Presentation] SARS-CoV全構造タンパク質発現型RVV免疫マウスでのSARS-CoV感染後に起きる肺炎増悪の解析 (2007)
  • Author(s): 安井文彦、甲斐知恵子、北畠正大、井上真吾、米田美佐子、横地祥司、加瀬良一、森田公一、松島綱治、志田壽利、水野喬介、小原道法
  • Organizer: 第11回日本ワクチン学会
  • Place of Presentation: 横浜
• [Presentation] Pathogenesis of SARS-CoV infection in BALB/c mice pre-immunized with recombinant vaccinia virus expressing SARS-CoV structural proteins (2007)
  • Author(s): Yasui F., Kai C., Kitabatake M., Inoue S., Yoneda M., Yokochi S., Kase R., Morita K., Matsushima K., Sida K., Mizuno K., Kohara M.
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Characterization of HCV Replication regulatory by DHCR24 (2007)
  • Author(s): Takano T., Hayashi M., Umehara T., Hirata Y., Tsukiyama-Kohara K., Kohara M
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Cyclophilins are essential for HCV RNA replication (2007)
  • Author(s): Matsuda C., Inoue K., Takahashi N., Sudoh M., Watanabe T., Yoshiba S., Kohara M
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Inter-genotype comparison of replicating and infecting abihties using partitioned-full genomic replicon system. (2007)
  • Author(s): Arai M.
  • Organizer: 14thInternational Symposiumon Hepatitis C Virus & Related Viruses
  • Place of Presentation: GLASGOW
• [Presentation] Hsp90 inhibitors suppress HCV replication in replicon cells and humanized-liver mice (2007)
  • Author(s): Nakagawa S.
  • Organizer: 14th International Symposiumon Hepatitis C Virus & Related Viruses
  • Place of Presentation: GLASGOW
• [Presentation] The cyclophillin inhibitor DERIO-025 shows anti-HCV effect in chimeric mouse with human hepatocytes (2007)
  • Author(s): Inoue K.
  • Organizer: 第66回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] Immunization of SARS-CoV nucleocapsid protein is involved in anaggravation of pulmonary inflammatory response to subsequent SARS-CoVinfection (2007)
  • Author(s): Yasui F.
  • Organizer: 第37回日本免疫学会
  • Place of Presentation: 東京
• [Presentation] Characterization of HCVReplication regulatory by DHCR24 (2007)
  • Author(s): Takano T.
  • Organizer: 第30回日本分子生物学会
  • Place of Presentation: 横浜
• [Book] C型肝炎ウイルス ; 宿主因子を標的にしたHCV増殖抑制・阻害を目指して (2008)
  • Author(s): 小原道法
  • Total Pages: 5
  • Publisher: ファルマシア
• [Book] 宿主因子を標的としたC型肝炎ウイルスの抑制 (2008)
  • Author(s): 平田雄一
  • Total Pages: 8
  • Publisher: ウイルス
• [Book] スフィンゴ脂質生成とC型肝炎ウイする増殖 (2007)
  • Author(s): 楳原 琢哉
  • Total Pages: 6
  • Publisher: BIOClinica
• [Book] Therapeutic application of RNA interference for hepatitis C virus (2007)
  • Author(s): Watanabe T.
  • Total Pages: 14
  • Publisher: Advanced Drug Delivery Reviews
• [Remarks]
  • URL: http://www.rinshoken.or.jp/
• [Remarks]
  • URL: http://www.rinshoken.or.jp/
• [Patent(Industrial Property Rights)] C型肝炎ウイルス阻害剤 (2008)
  • Inventor(s): 国立大学法人熊本大学 後藤正明、小原恭子 財団法人東京都医学研究機構 小原道法 財団法人化学及血清療法研究所 西村知裕
  • Industrial Property Rights Holder: 国立大学法人熊本大学 財団法人東京都医学研究機構、財団法人化学及血清療法研究所
  • Industrial Property Number: 2008-072420
  • Filing Date: 2008-03-14
• [Patent(Industrial Property Rights)] ベシクル製剤 (2008)
  • Inventor(s): 財団法人東京都医学研究機構 小原道法、北海道システム・サイエンス株式会社 中野善郎 中外製薬株式会社 須藤正幸
  • Industrial Property Rights Holder: 中外製薬株式会社、財団法人東京都医学研究機構、北海道システム・サイエンス株式会社
  • Industrial Property Number: 2008-066158
  • Filing Date: 2008-11-26
• [Patent(Industrial Property Rights)] C型肝炎ウイルスの働きを 阻害するオリゴリボヌクレオ チドまたはペプチド核酸 (2008)
  • Inventor(s): 財団法人東京都医学研究機構 小原道法 中外製薬株式会社 須藤正幸
  • Industrial Property Rights Holder: 中外製薬株式会社 財団法人東京都医学研究機構
  • Industrial Property Number: 2008-301764
  • Filing Date: 2008-11-26
• [Patent(Industrial Property Rights)] C型肝炎ウイルス遺伝子を有する組換ワクシニアウイルス (2008)
  • Inventor(s): 小原道法
  • Industrial Property Rights Holder: (財)東京都医学研究機構、(財)化学及血清療法研究所
  • Industrial Property Number: 2008-294361
  • Filing Date: 2008-11-18