• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

細胞膜に関連したエネルギー変換マシーナリーの構造・機能相関

Research Project

Project/Area Number 19042007
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo (2008)
Tokyo Institute of Technology (2007)

Principal Investigator

石谷 隆一郎  The University of Tokyo, 医科学研究所, 准教授 (30401186)

Co-Investigator(Kenkyū-buntansha) 濡木 理  東京大学, 医科学研究所, 教授 (10272460)
塚崎 智也  東京大学, 医科学研究所, 助教 (80436716)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 2008: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2007: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywords膜蛋白質 / 金属イオンの膜透過 / ペプチドの膜透過 / X線結晶構造解析 / ペプチドの膜組込み / 恒常性 / Sec蛋白質膜透過装置 / トランスロコン
Research Abstract

金属イオン輸送体の構造解析に関、しては、我々の結晶構造解析の結果(Nsyute2007)から示唆されたMg^<2+>依存的な構造変化を検証すべく, MgtE細胞質ドメインの分子動力学シミュレーションを行った.その結果, 計算機内で細胞質ドメインのMg^<2+>依存的な開閉構造変化を再現することに成功し, 細胞質ドメイン中のNドメインが構造変化に果たす役割が示唆された(PNAS, 2008). さらに結晶構造やシミュレーション結果から示唆されたMg2+ホメオスタシス機構を検証するために,パッチクランプ法等を用いた変異体解析を行った(論文準備中).
ペプチド膜透過装置に関しては、SecYEのセレノメチオニン置換体結晶を用いた多波長異常分散法により位相決定に成功し、最終的に3.2A分解能での構造決定を行った(Nature, 2008)。さらにTSecDFについては,ペリプラズム領域のみの高分解能構造を、結晶構造解析・NMR法を用いて決定した。今後全長の電子密度マップにこれらの構造を当てはめ、新規膜タンパク質TSecDFの分子モデルを構築中である。(論文準備中)

Report

(2 results)
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (16 results)

All 2009 2008 2007

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (2 results)

  • [Journal Article] Structural basis of tRNA modification with CO_2 fixation and methylation by wybutosine synthesizing enzyme TYW42009

    • Author(s)
      Suzuki Y, Noma A, Suzuki T, Is hitani R, Nureki O.
    • Journal Title

      Nucleic Acids Res. (未定)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray diffraction analysis of GCIP/HHM transcriptional regulator2009

    • Author(s)
      Seto A, (他7名) Ishitani R and Nureki O
    • Journal Title

      Acta Crystallogr Sect. F 65

      Pages: 21-24

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The structure of the pyrrolysyl-tRNA synthetase : tRNA^<pyl> complex reveals the molecular basis of orthogonality2009

    • Author(s)
      Nozawa K, (他3名) Ishitani R, & Soll_ D, Nureki O.
    • Journal Title

      Nature 457

      Pages: 1163-1167

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The structure of the pyrrolysyl-tRNA synthetase : tRNA^<pyl> compiex reveals the molecular basis of orthogonality2009

    • Author(s)
      Nozawa K, (他3名) Ishitani R, & Soli D, Nureki O.
    • Journal Title

      Nature 457

      Pages: 1163-1167

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure, dynamics, and function of RNA modification enzymes2009

    • Author(s)
      Ishitani R, Yokoyama S, & N ureki O.
    • Journal Title

      Curr. Opin. Struct. Biol. 18

      Pages: 330-339

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conformational transition of Sec machinery inferred from bacterial SecYE structures2008

    • Author(s)
      Tsukazaki T, (他2名), Ishitan i R, (他6名) & Nureki O.
    • Journal Title

      Nature 455

      Pages: 988-991

    • NAID

      10025975982

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mg^<2+>-sensing mechanism of Mg^<2+> transporter MgtE probed by molecular dynamics study2008

    • Author(s)
      Ishitani R, (他4名), & Nure ki O.
    • Journal Title

      Proc. Natl. Acad. Sci. U.S.A. 105

      Pages: 15393-15398

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Refinement of comparative models of protein structure by using multicanonical molecular dynamics simulations2008

    • Author(s)
      Ishitani R, Terada T, & Shimizu K.
    • Journal Title

      Mol. Simul. In press

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural insights into RNA-dependent eukaryal and archaeal selenocysteine formation2008

    • Author(s)
      Araiso Y, Palioura S, Ishitani R他8名
    • Journal Title

      Nucleic Acids Res. 36(4)

      Pages: 1187-1199

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary x-ray diffraction analysis of the cytosolic domain of a cation diffusion facilitator family protein2007

    • Author(s)
      Hattori M, Tanaka Y, Ishitani R, Nureki O
    • Journal Title

      Acta Crystallogr. F63

      Pages: 771-773

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA2007

    • Author(s)
      (5名) Ishitani R, Nioreki O
    • Journal Title

      J. Mol. Biol. 372(5)

      Pages: 1204-1214

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the MgtE Mg_<2+> transporter2007

    • Author(s)
      Hattori M, Tanaka y Fukai S, Ishitani R, Nureki O
    • Journal Title

      Nature 448(7157)

      Pages: 1072-1075

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary x-ray diffraction analysis of the full-length Mg_<2+> transporter MgtE2007

    • Author(s)
      Hattori M, Tanaka Y, Fukai S, Ishitani R, Nureki O
    • Journal Title

      Acta Crystallogr. F63

      Pages: 682-684

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the Sec4p.Sec2p complex in the nucleotide exchanging intermediate state2007

    • Author(s)
      Sato Y, Fukai S, Ishitani R, Nureki O
    • Journal Title

      Proc Natl Acad Sci U S A 104(20)

      Pages: 8305-8310

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] マグネシウム輸送体MgtEの分子動力学シミュレーション2008

    • Author(s)
      石谷隆一郎, 杉田有治, 濡木理
    • Organizer
      生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2008 Annual Research Report
  • [Presentation] マグネシウム輸送体MgtEの分子動力学シミュレーション2007

    • Author(s)
      石谷隆一郎, 杉田有給, 濡木理
    • Organizer
      生物物理学会第45回年会
    • Place of Presentation
      パシフイコ横浜
    • Year and Date
      2007-12-21
    • Related Report
      2007 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi