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Establishment of reverse genetics in medaka

Research Project

Project/Area Number 19101002
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionOsaka University

Principal Investigator

TODO Takeshi  大阪大学, 大学院・医学系研究科, 教授 (90163948)

Co-Investigator(Kenkyū-buntansha) MITANI Hiroshi  東京大学, 新領域創成科学研究科, 教授 (70181922)
YUBA Shunnsuke  独立行政法人産業技術総合研究所, 研究員 (40263248)
KAWASAKI Takashi  独立行政法人産業技術総合研究所, 研究員 (60356839)
Project Period (FY) 2007 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥107,640,000 (Direct Cost: ¥82,800,000、Indirect Cost: ¥24,840,000)
Fiscal Year 2011: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2010: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2009: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
Fiscal Year 2008: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
Fiscal Year 2007: ¥27,300,000 (Direct Cost: ¥21,000,000、Indirect Cost: ¥6,300,000)
Keywordsメダカ / 逆遺伝学 / 突然変異 / DNA損傷 / DNA修復 / 逆遺伝子 / 突然変異生成 / 組織特異的遺伝子発現 / 突然変異誘発
Research Abstract

To develop the system to study gene function at tissue level in medaka, we have established two methods in the present study. 1) Reverse genetics : TILLING(Targeting Induced Local Lesions IN Genome) is a reverse genetic strategy that combines random chemical mutagenesis with high-throughput discovery of induced mutations in the target genes. The method has been applied to a variety of plant and animal species. Screening of induced mutation is the most important step in TILLING. We developed the High Resolution Melting(HRM) assay, evaluated its efficacy for screening ENU-induced mutations in the medaka TILLING library. The HRM assay is fast, cost-effective, and robust in detecting variations in DNA. By HRM assay many nonsense mutations were identified and the phenotypes of these nonsense mutants confirmed their loss-of-function nature. 2) Regulation of gene expression in tissue : Infrared laser. evoked gene operator(IR-LEGO) is a microscope system optimized for heating cells at single cell level. Heat stress induces transcription of genes that are under the control of a heat shock promoter in individual targeted cells. We applied this technique to medaka fish carrying a heat shock promoter driven transgene and have succeeded the control of ectopic gene expression.

Report

(7 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (82 results)

All 2012 2011 2010 2009 2008 2007 Other

All Journal Article (40 results) (of which Peer Reviewed: 39 results) Presentation (38 results) Remarks (4 results)

  • [Journal Article] Involvement of the stress kinase mitogen-activated protein kinase kinase 7 in the regulation of the mammalian circadian clock2012

    • Author(s)
      Yoshimi Uchida, et.al.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Issue: 11 Pages: 8318-8326

    • DOI

      10.1074/jbc.m111.308908

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dmrt1 mutation causes a male-to-female sex reversal after the sex determination by Dmy in the medaka2011

    • Author(s)
      Masuyama H, Yamada M, Kamei Y, Fujiwara-Ishikawa T, Todo T, Nagahama Y, Matsuda M
    • Journal Title

      Chromosome Res

      Volume: 20(1) Issue: 1 Pages: 163-76

    • DOI

      10.1007/s10577-011-9264-x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A rapid and easy method forthe qualitative detection of intracellular deposition of inhaled nanoparticles2011

    • Author(s)
      Nakajima H, Ozaki K, Hongyo T, Narama I, Todo T
    • Journal Title

      Nanomedicine

      Volume: 7 Issue: 6 Pages: 881-888

    • DOI

      10.1016/j.nano.2011.02.004

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] FTIR study of light-dependent activation and DNA repair processes of (6-4) photolyase2011

    • Author(s)
      Zhang Y, et al
    • Journal Title

      Biochemistry

      Volume: 50 Issue: 18 Pages: 3591-3598

    • DOI

      10.1021/bi1019397

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 太陽光その恩恵と脅威2011

    • Author(s)
      藤堂剛
    • Journal Title

      環境と健康

      Volume: 24 Pages: 158-167

    • NAID

      130007000037

    • Related Report
      2011 Annual Research Report
  • [Journal Article] High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library2010

    • Author(s)
      Ishikawa T, Kamei Y, Otozai S, Kim J, Sato A, Kuwahara Y, Tanaka M, Deguchi T, Inohara H, Tsujimura T, Todo T
    • Journal Title

      BMC Mol Biol

      Volume: 11(1) Issue: 1 Pages: 70-70

    • DOI

      10.1186/1471-2199-11-70

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a functional medaka heat shock promoter and characterization of its ability to induce exogenous gene expression in medaka in vitro and in vivo2010

    • Author(s)
      Oda S, Mikami S, Urushihara Y, Murata Y, Kamei Y, Deguchi T, Kitano T, Fujimori KE, Yuba S, Todo T, Mitani H
    • Journal Title

      Zoolog Sci

      Volume: 27(5) Pages: 410-5

    • NAID

      40017358597

    • URL

      http://dx.doi.org/10.2108/zsj.27.410

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Salt-sensitive hypertension in circadian clock-deficient Cry-null mice involves dysreglated adrenal Hsd3b6.2010

    • Author(s)
      Masao Doi
    • Journal Title

      Nature Medicine

      Volume: 16 Pages: 67-74

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a functional medaka heat shock promoter and characterization of its ability to induce exogenous gene expression in medaka in vitro and in vivo.2010

    • Author(s)
      Shoji Oda
    • Journal Title

      Zoolog Sci.

      Volume: 27 Pages: 410-415

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of two members of the Cryptochrome/Photolyase Family from Ostreococcus tauri provides insights into the origin and evolution of cryptochromes.2010

    • Author(s)
      M Heijde
    • Journal Title

      Plant Cell Environ.

      Volume: 33 Pages: 1614-1626

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Loss of circadian rhythm and light-induced suppression of pineal melatonin levels in Cryl and Cry2 double-deficient mice.2010

    • Author(s)
      Yamanaka Y
    • Journal Title

      Genes Cells

      Volume: 15 Pages: 1063-1071

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library2010

    • Author(s)
      Tomoko Ishikawa
    • Journal Title

      BMC Mol Biol.

      Volume: 15

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diatom PtCPF1 is a new cryptochrome/ photolyase family member with DNA repair and transcription regulation activity2009

    • Author(s)
      Coesel S, Mangogna M, Ishikawa T, Heijde M, Rogato A, Finazzi G, Todo T, Bowler C, Falciatore A
    • Journal Title

      EMBO Rep

      Volume: 10(6) Issue: 6 Pages: 655-61

    • DOI

      10.1038/embor.2009.59

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kamei Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana2009

    • Author(s)
      Deguchi T, Itoh M, Urawa H, Matsumoto T, Nakayama S, Kawasaki T, Kitano T, Oda S, Mitani H, Takahashi T, Todo T, Sato J, Okada K, Hatta K, Yuba S
    • Journal Title

      Dev Growth Differ

      Volume: 51(9) Issue: 9 Pages: 769-75

    • DOI

      10.1111/j.1440-169x.2009.01135.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Common light signaling pathways controlling DNA repair and circadian clock entrainment in zebrafish2009

    • Author(s)
      Hirayama J, Miyamura N, Uchida Y, Asaoka Y, Honda R, Sawanobori K, Todo T, Yamamoto T, Sassone-Corsi P, Nishina H
    • Journal Title

      Cell Cycle

      Volume: 8(17) Pages: 2794-801

    • URL

      http://dx.doi.org/10.4161/cc.8.17.9447

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Iwai S, Panda S, Getzoff ED. Functional motifs in the(6-4) photolyase crystal structure make a comparative framework for DNA repair photolyases and clock cryptochromes2009

    • Author(s)
      Hitomi K, DiTacchio L, Arvai AS, Yamamoto J, Kim ST, Todo T, Tainer JA, Iwai S, Panda S, Getzoff ED
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 106(17) Issue: 17 Pages: 6962-7

    • DOI

      10.1073/pnas.0809180106

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Infrared laser-mediated gene induction in targeted single cells in vivo2009

    • Author(s)
      Kamei Y, Suzuki M, Watanabe K, Fujimori K, Kawasaki T, Deguchi T, Yoneda Y, Todo T, Takagi S, Funatsu T, Yuba S
    • Journal Title

      Nature Methods

      Volume: 6(1) Issue: 1 Pages: 79-81

    • DOI

      10.1038/nmeth.1278

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Salt-sensitive hypertension in circadian clock-deficient Cry-null mice involves dysreglated adrenal Hsd3b62009

    • Author(s)
      Doi M, Takahashi Y, Komatsu R, Yamazaki F, Yamada H, Haraguchi S, Emoto N, Okuno Y, Tsujimoto G, Kanematsu A, Ogawa O, Todo T, Tsutsui K, van der Horst GT, Okamura H.
    • Journal Title

      Nature Medicine 16

      Pages: 67-74

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Infred laser-mediated local gene induction in medaka, zebrafish and Arabidopsis taliana2009

    • Author(s)
      Deguchi T, Itoh M, Urawa H, Matsumoto T, Nakayama S, Kawasaki T, Kitano T, Oda S, Mitani H, Takahashi T, Todo T, Sato J, Okada K, Hatta K, Yuba S, Kamei Y.
    • Journal Title

      Dev. Growth Differ. 51

      Pages: 769-775

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Infred laser-mediated gene induction in targeted single cells in vivo2009

    • Author(s)
      Kamei Y, Suzuki M, Watanabe K, Fujimori K, Kawasaki T, Deguchi T, Yoneda Y, Todo T, Takagi S, Funatsu T, Yuba S.
    • Journal Title

      Nature Methods 6

      Pages: 79-81

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Salt-sensitive hypertension in circadian clock-deficient Cry-null mice involves dysreglated adrenal Hsd3b6.2009

    • Author(s)
      Masao Doi
    • Journal Title

      Nature Medicine 16

      Pages: 67-74

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Infred laser-mediated local gene induction in medaka, zebrafish and Arabidopsis taliana.2009

    • Author(s)
      Tomonori Deguchi
    • Journal Title

      Dev.Growth Differ. 51

      Pages: 769-775

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Common light signaling pathways controlling DNA repair and circadian entrainment in zebrafish.2009

    • Author(s)
      Jun Hirayama
    • Journal Title

      Cell Cycle 8

      Pages: 2794-2801

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diatom PtCPF1 is a new cryptochrome/Photolyase family member with DNA repair and transcription regulation activity.2009

    • Author(s)
      Sacha Coesel
    • Journal Title

      EMBO reports 10

      Pages: 655-661

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional motif in the (6-4) photolyase crystal structure : A comparative framework for DNA repair photolyase and clock cryptochromes.2009

    • Author(s)
      Kenichi Hitomi
    • Journal Title

      Proc.Natl.Acad.Sci.USA 106

      Pages: 6962-6967

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Infred laser-mediated gene induction in targeted single cells in vivo.2009

    • Author(s)
      Yasuhiro Kamei
    • Journal Title

      Nature Methods 6

      Pages: 79-81

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Infrared laser-mediated gene induction in targeted single cells in vivo.2009

    • Author(s)
      Yasuhiro Kamei
    • Journal Title

      Nat Methods. 6

      Pages: 79-81

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional motifs in the (6-4)photolyase crystal structure : A comparative framework for DNA repair photolyase and clock cryptochromes2009

    • Author(s)
      Kenichi Hitomi
    • Journal Title

      Proc. Natl. Acad. Sci. USA 106(In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex2008

    • Author(s)
      Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T
    • Journal Title

      J Biol Chem

      Volume: 283(14) Issue: 14 Pages: 9071-9

    • DOI

      10.1074/jbc.m709835200

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryptochrome is present in the compound eyes and a subset of Drosophila's clock neurons.2008

    • Author(s)
      Taishi Yoshii
    • Journal Title

      J Comp Neurol. 508

      Pages: 952-966

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of electron transfer in the photoreaction of zebrafish Cryptochrome-DASH.2008

    • Author(s)
      Kazunori Zikihara
    • Journal Title

      Photochem Photobiol 84

      Pages: 1016-1023

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diatom PtCPF1 is a novel Cryptochrome/Photolyase Family member with DNA repair and transcription regulation activity2008

    • Author(s)
      Sacha Coesel
    • Journal Title

      EMBO Report 10(In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage induced ubiquitylation of RFC2 subunit of RFC complex.2008

    • Author(s)
      Tomida J
    • Journal Title

      J Biol Chem (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus2007

    • Author(s)
      Kamei Y, Itou J, Oda S, Masui M, KimJH, Ishikawa T, Yuba S, Kinoshita M, Mitani H, Todo T
    • Journal Title

      Dev Growth Differ

      Volume: 49(9) Pages: 721-30

    • NAID

      10019984185

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A model for triplet mutation formation based on error-prone translesional DNA synthesis opposite UV photolesions2007

    • Author(s)
      Ikehata H., Ono T., Tanaka K., Todo T
    • Journal Title

      DNA Repair

      Volume: 6(5) Pages: 658-68

    • URL

      http://dx.doi.org/10.1016/j.dnarep.2006.12.011

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] ENDOR differentiates complementary roles for active site histidines in (6-4) photolyase2007

    • Author(s)
      Schleicher E
    • Journal Title

      J Biol Chem 282

      Pages: 4738-4747

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autonomic and cardiovascular responses to scent stimulation are altered in cry KO mice2007

    • Author(s)
      Tanida M
    • Journal Title

      Neurosci Lett. 413

      Pages: 177-182

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A model for triplet mutation formation based on error-prone translesional DNA synthesis opposite UV photolesions2007

    • Author(s)
      Ikehata H
    • Journal Title

      DNA Repair 6

      Pages: 658-668

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clock gene defect disrupts light-dependency of autonomic nerve activity2007

    • Author(s)
      Ikeda H
    • Journal Title

      Biochem Biophys Res Commun. 364

      Pages: 457-463

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a convenient in vitro fertilization method using interspecific hybrids between Oryzias latipes and Oryzias curvinotus.2007

    • Author(s)
      Kamei Y
    • Journal Title

      Dev Growth Differ 49

      Pages: 721-730

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] High throughput screening of induced mutations in Medaka TILLING library2011

    • Author(s)
      Tomoko Fujiwara-Ishikawa, Takeshi Todo
    • Organizer
      The 1st Strategic Meeting for Medaka Research
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2011-11-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] ATF6 is essential for induction of ER chaperones required for early development2011

    • Author(s)
      Tokiro Ishikawa, et al
    • Organizer
      The 1st Strategic Meeting for Medaka Research
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2011-11-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of association between GBA mutation and Parkinson's disease using medaka fish2011

    • Author(s)
      Norihito Uemura, et al
    • Organizer
      The 1st Strategic Meeting for Medaka Research
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2011-11-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] メダカTILLINGライブラリーの網羅的スクリーニング法の確立2011

    • Author(s)
      藤原(石川)智子、藤堂剛
    • Organizer
      日本放射線影響学会第54回大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2011-11-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] A single basepair difference is sufficient to distinguish males from females in medaka fish2011

    • Author(s)
      Haruo Masuyama, et al
    • Organizer
      第17回小型魚類研究会
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2011-09-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] High throughput screening of induced mutations in Medaka TILLING library2011

    • Author(s)
      Tomoko Fujiwara-Ishikawa, Takeshi Todo
    • Organizer
      第17回小型魚類研究会
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] ATF6 is essential for induction of ER chaperones required for early development2011

    • Author(s)
      Tokiro Ishikawa, et al
    • Organizer
      第17回小型魚類研究会
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of ERAD mechanism by using transgenic and knockout Medaka2011

    • Author(s)
      Taiki Hara, et al
    • Organizer
      第17回小型魚類研究会
    • Place of Presentation
      愛知県岡崎市
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Heavy ion radiation effects on spermatogenesis in Medaka (Oryzias latipes) p53 nonsense mutant2011

    • Author(s)
      Hiroshi Mitani, et al
    • Organizer
      14^<th> International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Ascorbic acid suppresses hair depigmentation caused by irradiation2011

    • Author(s)
      Tadashi Hongyo, et al
    • Organizer
      14^<th> International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] In vivo distribution of radionuclides, tumorigenicity and genome stress in mice after Chernobyl catastrophe2011

    • Author(s)
      Hiroo Nakajima, et al
    • Organizer
      14^<th> International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Isolation and character ization of p53, ATM and ATR mutants in medaka fish2011

    • Author(s)
      Tomoko Fujiwara. Ishikawa, Takeshi Todo et al
    • Organizer
      14^<th> International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Isolation and characterization of p53, ATM and ATR mutants in medaka fish2011

    • Author(s)
      Tomoko Fujiwara-Ishikawa, Takeshi Todo, et al
    • Organizer
      14^<th> International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] p53 dependent suppression of Genome Instability in Medaka germ cell2011

    • Author(s)
      Takeshi Todo, Tomoko Fujiwara-Ishikawa, et al
    • Organizer
      14^<th> International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] High throughput screening of induced mutations in Medaka TILLING library2011

    • Author(s)
      Tomoko Fujiwara. Ishikawa, Takeshi Todo
    • Organizer
      5^<th> Asia and Oceania Conf erence for Photobiology
    • Place of Presentation
      奈良県奈良市
    • Year and Date
      2011-07-31
    • Related Report
      2011 Final Research Report
  • [Presentation] High throughput screening of induced mutations in Medaka TILLING library2011

    • Author(s)
      Tomoko Fujiwara-Ishikawa, Takeshi Todo
    • Organizer
      5^<th> Asia and Oceania Conference for Photobiology
    • Place of Presentation
      奈良県奈良市
    • Year and Date
      2011-07-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Comparison of molecular properties between two functionally distinct Ciyptochrome-DASH proteins2011

    • Author(s)
      Kazunori Zikihara, et al
    • Organizer
      5^<th> Asia and Oceania Conference for Photobiology
    • Place of Presentation
      奈良県奈良市
    • Year and Date
      2011-07-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Spectroscopic study of the photoactivation process of Xenopus (6-4) photolyase2011

    • Author(s)
      Daichi Yamada, et al
    • Organizer
      5^<th> Asia and Oceania Conference for Photobiology
    • Place of Presentation
      奈良県奈良市
    • Year and Date
      2011-07-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Photolyase/Cryptochrome family-Introduction2011

    • Author(s)
      Takeshi Todo
    • Organizer
      5^<th> Asia and Oceania Conference for Photobiology
    • Place of Presentation
      奈良県奈良市(招待講演)
    • Year and Date
      2011-07-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] マイクロサテライトを指標としたメダカにおけるゲノム不安定性の解析2010

    • Author(s)
      石川智子
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      京都市
    • Year and Date
      2010-10-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] scidマウスと野生型マウス系統におけうるγ-H2AXフォーカス消長の比較2010

    • Author(s)
      中島裕夫
    • Organizer
      日本放射線影響学会
    • Place of Presentation
      京都市
    • Year and Date
      2010-10-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] メダカATM, ATR変異体の解析、日本放射線影2009

    • Author(s)
      石川智子, 亀井保博、藤堂剛
    • Organizer
      響学会第52回大会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2009-11-12
    • Related Report
      2011 Final Research Report
  • [Presentation] メダカATM,ATR変異体の解析2009

    • Author(s)
      石川智子
    • Organizer
      日本放射線影響学会第52回大会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2009-11-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] TILLING法による遺伝子破壊メダカ作製の現状2009

    • Author(s)
      谷口善仁, 武田俊一, 石川智子, 藤堂剛, 亀井保博, 音在信治
    • Organizer
      第80回日本動物学会
    • Place of Presentation
      静岡県コンベンションアーツセンターグランシップ
    • Year and Date
      2009-09-18
    • Related Report
      2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] メダカ逆遺伝学的手法による変異個体を用いた突然変異生成の分子遺伝学的解析2009

    • Author(s)
      石川智子, 亀井保博, 音在信治, 藤堂剛
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋大学野依記念学術交流会館
    • Year and Date
      2009-09-12
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] メダカ逆遺伝学的手法による変異個体を用いた突然変異生成の分子遺伝学的解析2009

    • Author(s)
      石川智子
    • Organizer
      第15回小型魚類研究会
    • Place of Presentation
      名古屋大学野依記念学術交流?会館
    • Year and Date
      2009-09-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] 多機能性新規クリプトクローム/光回復酵素ファミリーメンバー2009

    • Author(s)
      Sacha Coesel
    • Organizer
      日本光生物学協会第15回大会
    • Place of Presentation
      自然科学研究機構岡崎コンフェレンスセンター
    • Year and Date
      2009-08-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] メダカにおける逆遺伝学的手法の確立:突然変異生成機構の分子遺伝学的解析を目指して2009

    • Author(s)
      石川智子
    • Organizer
      変異機構研究会第22回夏の学校
    • Place of Presentation
      愛知県小牧勤労センター
    • Year and Date
      2009-06-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] Establishment of Reverse Genetics in Medaka : Isolation and characterization of UV-sensitive mutant.2008

    • Author(s)
      Tomoko Ishikawa
    • Organizer
      4^<th> Asia Oceania Conference on Photobiology
    • Place of Presentation
      Varanasi, India
    • Year and Date
      2008-11-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] 突然変異生成の分子遺伝学的解析2008

    • Author(s)
      石川智子
    • Organizer
      日本放射線影響学会第51回大会
    • Place of Presentation
      小倉
    • Year and Date
      2008-11-20
    • Related Report
      2008 Annual Research Report
  • [Presentation] Establishment of Reverse Genetics in Medaka2008

    • Author(s)
      Todo T
    • Organizer
      The Fourth Aquatic Animal Models of Human Disease co nference
    • Place of Presentation
      North Carolina USA
    • Year and Date
      2008-02-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Establishment of Reverse Genetics in Medaka2008

    • Author(s)
      Todo T.
    • Organizer
      The Fourth Aquatic Animal Models of Human Disease conference
    • Place of Presentation
      Durham, North Carolina USA
    • Year and Date
      2008-02-01
    • Related Report
      2007 Annual Research Report
  • [Presentation] メダカにおける逆遺伝学的手法を用いた変異体の作製2007

    • Author(s)
      石川 智子
    • Organizer
      日本放射線影響学会第50回大会
    • Place of Presentation
      千葉
    • Year and Date
      2007-11-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] DNA損傷によって誘導されるRFC複合体のユビキチン化2007

    • Author(s)
      冨田 純也
    • Organizer
      日本放射線影響学会第50回大会
    • Place of Presentation
      千葉
    • Year and Date
      2007-11-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] メダカにおける逆遺伝学的手法の確立:光生物学への応用を目指して2007

    • Author(s)
      石川智子
    • Organizer
      第14回光生物協会年会
    • Place of Presentation
      奈良
    • Year and Date
      2007-07-31
    • Related Report
      2007 Annual Research Report
  • [Presentation] 魚類培養細胞における光応答メカニズム2007

    • Author(s)
      石川智子
    • Organizer
      第14回光生物協会年会
    • Place of Presentation
      奈良
    • Year and Date
      2007-07-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Screening of induced pointmutations in Medaka with TILING2007

    • Author(s)
      Todo T
    • Organizer
      13^<th> International congress of Radiat ion Research
    • Place of Presentation
      San Francisco USA
    • Year and Date
      2007-07-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Screening of induced point mutations in Medaka with TILING2007

    • Author(s)
      Todo T.
    • Organizer
      13th International congress of Radiation Research
    • Place of Presentation
      San Francisco USA
    • Year and Date
      2007-07-10
    • Related Report
      2007 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/radbio/www/index-jp.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/radbio/www/index-jp.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/radbio/www/index-jp.html

    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/radbio/www/index-jp.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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