Molecular Design of Oxygenases Applicable to Synthetic Chemistry

• Project/Area Number: 19105004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Nagoya University
• Principal Investigator: WATANABE Yoshihito 名古屋大学, 物質科学国際研究センター, 教授 (10201245)
• Co-Investigator(Kenkyū-buntansha): 中島 洋 名古屋大学, 大学院・理学研究科, 准教授 (00283151) ; 永野 慎吾 独立行政法人理化学研究所, 城生体金属化学研究室, 専任研究員 (60286440)
• Co-Investigator(Renkei-kenkyūsha): NAKAGIMA Hiroshi 名古屋大学, 大学院・理学研究科, 准教授 (00283151) ; NAGANO Shingo 鳥取大学, 大学院・工学研究科, 教授 (60286440)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥111,150,000 (Direct Cost: ¥85,500,000、Indirect Cost: ¥25,650,000); Fiscal Year 2011: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2010: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2009: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2008: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2007: ¥38,090,000 (Direct Cost: ¥29,300,000、Indirect Cost: ¥8,790,000)
• Keywords: 生物無機化学 / ヘム蛋白質 / シトクロムP450 / 酸化反応 / 過酸化水素 / 水酸化反応 / P450 / エポキシ化反応

Research Abstract

The study has achieved creation of novel artificial oxygenases founded on a heme protein deriving from a thermophile or a cytochrome P450 protein that utilizes H2O2 as the oxidant. A newly developed method,"Decoy molecule" succeeded to rationally modify the active site of the P450 protein in addition to conventional mutagenesis techniques. The artificial oxygenases exerted high catalytic activity and/or thermal tolerance which were not achieved by ever reported artificial oxygenases. Results in the study provide ideas and methodology to design artificial oxygenases aiming practical uses and might be applicable to artificial enzymes of other activities.

Research Products

• [Journal Article] Construction of Biocatalysts Using the Myoglobin Scaffold for the Synthesis of Indigo from Indole (2012)
  • Author(s): J. Xu, O. Shoji, T. Fujishiro, T. Ohki, T. Ueno, Y. Watanabe
  • Journal Title: Catalysis Science & Technology Volume: 2 Issue: 4 Pages: 739-744
  • DOI: 10.1039/c2cy00427e
  • Peer Reviewed
• [Journal Article] Protein Photoconductors and Photodiodes (2011)
  • Author(s): Y. Tokita, Y. Goto, S. Yamada, T. Luo, H. Nakajima, Y. Watanabe
  • Journal Title: Angew. Chem. Int. Ed. Volume: 50 Issue: 49 Pages: 11663-11666
  • DOI: 10.1002/anie.201103341
  • Peer Reviewed
• [Journal Article] Cytochrome c552 from Thermus Thermophilus Engineered for Facile Substitution of Prosthetic Group (2011)
  • Author(s): Sk. Md. Ibrahim, H. Nakajima, K. Ramanathan, N. Takatani, T. Ohta, Y. Naruta, Y. Watanabe
  • Journal Title: Biochemistry Volume: 50(45) Issue: 45 Pages: 9826-9835
  • DOI: 10.1021/bi201048e
  • Peer Reviewed
• [Journal Article] Crystal Structure of H2O2-dependent Cytochrome P450SPαwith its Bound Fatty Acid Substrate : Insight into the Regioselective Hydroxylation of Fatty Acids at theαPosition (2011)
  • Author(s): T. Fujishiro, O. Shoji, S. Nagano, H. Sugimoto, Y. Shiro, Y. Watanabe
  • Journal Title: J. Biol. Chem. Volume: 286 Issue: 34 Pages: 29941-29950
  • DOI: 10.1074/jbc.m111.245225
  • Peer Reviewed
• [Journal Article] Use of Perfluoro Carboxylic Acids Trick Cytochrome P450BM3 into Initiating Hydroxylation of Gaseous Alkanes (2011)
  • Author(s): N. Kawakami, O. Shoji, Y. Watanabe
  • Journal Title: Angew. Chem. Int. Ed. Volume: 50 Issue: 23 Pages: 5315-5318
  • DOI: 10.1002/anie.201007975
  • Peer Reviewed
• [Journal Article] Design of H2O2-Dependent Oxidation Catalyzed by Hemoproteins (2011)
  • Author(s): O. Shoji, Y. Watanabe
  • Journal Title: Metallomics Volume: 3 Issue: 4 Pages: 379-388
  • DOI: 10.1039/c0mt00090f
  • Peer Reviewed
• [Journal Article] Molecular Design of Heme Proteins for Future Application (2011)
  • Author(s): H. Nakajima, O. Shoji, Y. Watanabe
  • Journal Title: Catalysis Surveys from Asia Volume: 15 Issue: 2 Pages: 134-143
  • DOI: 10.1007/s10563-011-9117-9
  • Peer Reviewed
• [Journal Article] Non-covalent Modification of the Active Site of Cytochrome P450 for Inverting the Stereoselectivity of Monooxygenation (2011)
  • Author(s): T. Fujishiro, O. Shoji, Y. Watanabe
  • Journal Title: Tetrahedron Lett. Volume: 52,(3) Issue: 3 Pages: 395-397
  • DOI: 10.1016/j.tetlet.2010.11.048
  • Peer Reviewed
• [Journal Article] The role of the GAF and central domains of the transcriptional activator VnfA in Azotobacter vinelandii (2011)
  • Author(s): K.Yoshimitsu, N.Takatani, Y.Miura, Y.Watanabe, H.Nakajima
  • Journal Title: FEBS Journal Volume: 278 Issue: 18 Pages: 3287-3297
  • DOI: 10.1111/j.1742-4658.2011.08245.x
  • Peer Reviewed
• [Journal Article] Design of H_2O_2-Dependent Oxidation Catalyzed by Hemoproteins (2011)
  • Author(s): O.Shoji, Y.Watanabe
  • Journal Title: Metallomics Volume: 3(印刷中,未定) Pages: 379-388
  • Peer Reviewed
• [Journal Article] Non-covalent modification of the active site of cytochrome P450 for inverting the stereoselectivity of monooxygenation (2011)
  • Author(s): T.Fujishiro, O.Shoji, Y.Watanabe
  • Journal Title: Tetrahedron Lett. Volume: 52 Pages: 395-397
  • Peer Reviewed
• [Journal Article] Definite Coordination Arrangement of Organometallic Palladium Complexes Accumulated on the Designed Interior Surface of Apo-ferritin (2011)
  • Author(s): Z.Wang, Y.Takezawa, H.Aoyagi, S.Abe, T.Hikage, Y.Watanabe, S.Kitagawa, T.Ueno
  • Journal Title: Chem.Commun. Volume: 47 Pages: 170-172
  • Peer Reviewed
• [Journal Article] Dual modification of a triple-stranded b-helix nanotube with Ru and Re metal complexes to promote photocatalytic reduction of CO2 (2011)
  • Author(s): N.Yokoi, Y.Miura, C.-Y.Huang, N.Takatani, H.Inaba, T.Koshiyama, S.Kanamaru, F.Arisaka, Y.Watanabe, S.Kitagawa, T.Ueno
  • Journal Title: Chem. Commun. Volume: 47 Pages: 2074-2076
  • Peer Reviewed
• [Journal Article] Incorporation of Organometallic Ru Complexes into Apo-Ferritin Cage (2011)
  • Author(s): Y.Takezawa, P.Bockmann, N.Sugi, Z.Wang, S.Abe, T.Murakami, T.Hikage, G.Erker, Y.Watanabe, S.Kitagawa, T.Ueno
  • Journal Title: Dalton.Trans. Volume: 40 Pages: 2190-2195
  • Peer Reviewed
• [Journal Article] Understanding Substrate Misrecognition of Hydrogen Peroxide-Dependent Cytochrome P450 from Bacillus Subtilis (2010)
  • Author(s): O. Shoji, T. Fujishiro, S. Nagano, S. Tanaka, T. Hirose, Y. Shiro, Y. Watanabe
  • Journal Title: J. Biol. Inorg. Chem. Volume: 15,(8) Issue: 8 Pages: 1331-1339
  • DOI: 10.1007/s00775-010-0692-4
  • Peer Reviewed
• [Journal Article] Aromatic C-H bond Hydroxylation by P450 Peroxygenases : A Facile Colorimetric Assay for Monooxygenation Activities of Enzymes Based on the Russig's Blue Formation (2010)
  • Author(s): O. Shoji, C. Wiese, T. Fujishiro, C. Shirataki, B. Wunsch, Y. Watanabe
  • Journal Title: J. Biol. Inorg. Chem. Volume: 15,(7) Issue: 7 Pages: 1109-1115
  • DOI: 10.1007/s00775-010-0671-9
  • Peer Reviewed
• [Journal Article] Rational Engineering of Thermus Thermophilus Cytochrome c552 to a Thermally Tolerant Artificial Peroxidase (2010)
  • Author(s): H. Nakajima, K. Ramanathan, N. Kawaba, Y. Watanabe
  • Journal Title: Dalton Trans. Volume: 39 Issue: 12 Pages: 3105-3114
  • DOI: 10.1039/b924365h
  • Peer Reviewed
• [Journal Article] Understanding Substrate Misrecognition of Hydrogen Peroxide-Dependent Cytochrome P450 from Bacillus Subtilis (2010)
  • Author(s): O.Shoji, T.Fujishiro, S.Nagano, S.Tanaka, T.Hirose, Y.Shiro, Y.Watanabe
  • Journal Title: J.Biol.Inorg.Chem. Volume: 15 Pages: 1331-1339
  • Peer Reviewed
• [Journal Article] Aromatic C-H bond hydroxylation by P450 peroxygenases : A facile colorimetric assay for monooxygenation activities of enzymes based on the Russig's Blue formation (2010)
  • Author(s): O.Shoji, C.Wiese, T.Fujishiro, C.Shirataki, B.W nsch, Y.Watanabe
  • Journal Title: J.Biol.lnorg.Chem. Volume: 15 Pages: 1109-1115
  • Peer Reviewed
• [Journal Article] Construction of Robust Bio-nanotube by Controlled Self-assembly of Component Proteins of Bacteriophege T4 (2010)
  • Author(s): N.Yokoi, H.Inaba, M.Terauchi, A.Z.Stieg, N.J.M.Sanghamitra, T.Koshiyama, K.Yutani, S.Kanamaru, F.Arisaka, T.Hikage, A.Suzuki, T.Yamane, J.K.Gimzewski, Y.Watanabe, S.Kitagawa, T.Ueno
  • Journal Title: Small Volume: 6 Pages: 1873-1879
  • Peer Reviewed
• [Journal Article] Mechanism of Accumulation and Incorporation of Organometallic Pd Complexes into the Protein Nanocage of apo-Ferritin (2010)
  • Author(s): S.Abe, T.Hikage, Y.Watanabe, S.Kitagawa, T.Ueno
  • Journal Title: Inorg.Chem. Volume: 49 Pages: 6967-6973
  • Peer Reviewed
• [Journal Article] Rational engineering of Thermus thermophilus cytochrome c_<552> to a thermally tolerant artificial peroxidase (2010)
  • Author(s): 中島洋, Ramanathan Kalaivani, 川場直美, 渡辺芳人
  • Journal Title: Dalton Transactions 39 Pages: 3105-3114
  • Peer Reviewed
• [Journal Article] The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii (2010)
  • Author(s): H.Nakajima, N.Takatani, K.Yoshimitsu, M.Itoh, S.Aono, Y.Takahashi, Y.Watanabe
  • Journal Title: FEBS Journal 277 Pages: 817-832
  • Peer Reviewed
• [Journal Article] Elucidation of Metal-Ion Accumulation Induced by Hydrogen Bonds on Protein Surfaces by Using Porous Lysozyme Crystals Containing RhIIIIons as the Model Surfaces (2010)
  • Author(s): T.Ueno, S.Abe, T.Koshiyama, T.Ohki, T.Hikage, Y.Watanabe
  • Journal Title: Chem.Eur.J. 16 Pages: 2730-2740
  • Peer Reviewed
• [Journal Article] Modification of Porous Protein Crystals in Development of Biohybrid Materials (2010)
  • Author(s): T.Koshiyama, N.Kawaba, T.Hikage, M.Shirai, Y.Miura, Cheng-Yuan Huang, K.Tanaka, Y.Watanabe, T.Ueno
  • Journal Title: Bioconjugate Chem. 21 Pages: 264-269
  • Peer Reviewed
• [Journal Article] Preparation and catalytic reaction of Au/Pd bimetallic nanoparticles in Apo-Ferritin (2009)
  • Author(s): M.Suzuki, M.Abe, T.Ueno, S.Abe, T.Goto, Y.Toda, T.Akita, Y.Yamada, Y.Watanabe
  • Journal Title: Chem.Commun. 32 Pages: 4871-4873
  • Peer Reviewed
• [Journal Article] Construction of an Energy Transfer System in the Bio-nanocup Space by Heteromeric Assembly of gp27 and gp5 Protein Isolated from Bacteriophage T4 (2009)
  • Author(s): T.Koshiyama, T.Ueno, S.Kanamaru, F.Arisaka, Y.Watanabe
  • Journal Title: Org.Biomol.Chem. 7 Pages: 2649-2654
  • Peer Reviewed
• [Journal Article] Polymerization of Phenylacetylene by Rhodium Complexes within a Discrete Space of apo-Ferritin (2009)
  • Author(s): S.Abe, K.Hirata, T.Ueno, K.Morino, N.Shimizu, M.Yamamoto, M.Takata, E.Yashima, Y.Watanabe
  • Journal Title: J.Am.Chem.Soc. 131 Pages: 6958-6960
  • Peer Reviewed
• [Journal Article] Process of Accumulation of Metal Ions on the Interior Surface of apo-Ferritin : Crystal Structures of a Series of apo-Ferritins Containing Variable Quantities of Pd(II) Ions (2009)
  • Author(s): T.Ueno, M.Abe, K.Hirata, S.Abe, M.Suzuki, N.Shimizu, M.Yamamoto, M.Takata, Y.Watanabe
  • Journal Title: J.Am.Chem.Soc. 131 Pages: 5094-5100
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 渡辺芳人, 上野隆史
  • Journal Title: 配位空間の化学―最新技術と応用―(シーエムシー出版) Pages: 301-311
• [Journal Article] (2009)
  • Author(s): 渡辺芳人, 安部聡, 上野隆史
  • Journal Title: ナノ空間材料の創製と応用(フロンティア出版) Pages: 72-79
• [Journal Article] Process of Accumulation of Metal Ions on the Interior Surface of apo-Ferritin : Crystal Structures of a Series of apo-Ferritins Containing Variable Quantities of Pd (II) Ions (2009)
  • Author(s): T. Ueno, M. Abe, K. Hirata, S. Abe, M. Suzuki, N. Shimizu, M. Yamamoto, M. Takata, Y. Watanabe
  • Journal Title: J. Am. Chem. Soc. 131 Pages: 5094-5100
  • Peer Reviewed
• [Journal Article] Engineering of Thermus Thermophilus Cytochrome c552 : Thermally Tolerant Artificial Peroxidase (2008)
  • Author(s): H. Nakajima, Y. Ichikawa, Y. Satake, N. Takatani, S. K. Manna, J. Rajbongshi, S. Mazumdar, Y. Watanabe
  • Journal Title: ChemBioChem. Volume: 9 Issue: 18 Pages: 2954-2957
  • DOI: 10.1002/cbic.200800599
  • Peer Reviewed
• [Journal Article] Mechanism of Intermolecular electron Transfer in the Photoexcited Zn-Substituted Cytochrome c : Theoretical and Experimental Perspective (2008)
  • Author(s): Y. Tokita, J. Shimura, H. Nakajima, Y. Gotoh, Y. Watanabe
  • Journal Title: J. Am. Chem. Soc. Volume: 130 Issue: 15 Pages: 5302-5310
  • DOI: 10.1021/ja711324t
  • Peer Reviewed
• [Journal Article] Engineering of Thermus thermophilus cytochrome c_<552> : Thermally tolerant artificial peroxidase (2008)
  • Author(s): 中島洋, 市川祐介, 佐竹由宇, 高谷信之, Manna Kanti Soumen, Rajbongshi Jitumani, Mazumdar Shyamalava, 渡辺芳人
  • Journal Title: ChemBioChem 9 Pages: 2954-2957
  • Peer Reviewed
• [Journal Article] Mechanism of Intermolecular Electron Transfer in the Photoexcited Zn-Substituted Cytochrome c : Theoretical and Experimental Perspective (2008)
  • Author(s): Y. Tokita, J. Shimura, H. Nakajima, Y. Goto, Y. Watanabe
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 5302-5310
  • Peer Reviewed
• [Journal Article] Control of the Coordination Structure of Organometallic Palladium Complexes in an apo-Ferritin Cage (2008)
  • Author(s): S. Abe, J. Niemeyer, A. Abe, Y. Takezawa, T. Ueno, T. Hikage, G. Erker, Y. Watanabe
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 10512-10514
  • Peer Reviewed
• [Journal Article] Engineering of Thermus Thermophilus Cytochrome c552 : Thermally Tolerant Artificial Peroxidase (2008)
  • Author(s): H. Nakajima, Y. Ichikawa, Y. Satake, N. Takatani, S. K. Manna, J. Rajbongshi, S. Mazumdar, Y. Watanabe
  • Journal Title: ChemBioChem 9 Pages: 2954-2957
  • Peer Reviewed
• [Journal Article] Noncovalent insertion of ferrocenes into the protein shell of apo-ferritin (2008)
  • Author(s): J. Niemeyer, S. Abe, T. Hikage, T. Ueno, G. Erker, Y. Watanabe
  • Journal Title: Chem. Commun. 48 Pages: 6519-6521
  • Peer Reviewed
• [Journal Article] 有機金属酵素創製の試み (2008)
  • Author(s): 渡辺芳人
  • Journal Title: Organo Metallic NEWS Pages: 158-164
• [Journal Article] Molecular Design of Heteroprotein Assemblies ProViding a Bionanocup as a Chemical Reactor (2008)
  • Author(s): T. Koshiyama, N. Yokoi, T. Ueno, S. Kanamaru, S. Nagano, Y. Shiro, F. Arisaka, and Y. Watanabe
  • Journal Title: Small 4 Pages: 50-54
  • Peer Reviewed
• [Journal Article] Ligand Design for the Improvement of Stability of Metal Complex-Protein Hybrid (2008)
  • Author(s): N. Yokoi, T. Ueno, T. Matsui, M. Ikeda-Saito, and Y. Watanabe
  • Journal Title: Chem. Commun. Pages: 229-231
  • Peer Reviewed
• [Journal Article] Reactivities of Oxo and Peroxo Intermediates Studied by Hemoprotein Mutants (2007)
  • Author(s): Y. Watanabe, H. Nakajima, T. Ueno
  • Journal Title: Acc. Chem. Res. Volume: 40 Issue: 7 Pages: 554-562
  • DOI: 10.1021/ar600046a
  • Peer Reviewed
• [Journal Article] Incorporation of Phebox Rhodium Complex into apo-Myoglobin Affords Stable Organometallic Protein Showing Unprecedented Arrangement of the Complex in the Cavity (2007)
  • Author(s): Y. Satake, S. Abe, S. Okazaki, N. Ban, T. Hikage, T. Ueno, H. Nakajima, A. Suzuki, T. Yamane, H. Nishiyama, Y. Watanabe
  • Journal Title: Organometallics. Volume: 26 Issue: 20 Pages: 4904-4908
  • DOI: 10.1021/om700471a
  • Peer Reviewed
• [Journal Article] Design and Structure Analysis of Artificial Metalloproteins : Selective Coordination of His64 to Copper Complexes with Square-Planar Structure in the Apo-Myoglobin Scaffold (2007)
  • Author(s): S. Abe, T. Ueno, P. Reddy, S. Okazaki, T. Hikage, A. Suzuki, T. Yamane, H. Nakajima, Y. Watanabe
  • Journal Title: Inorg. Chem. Volume: 46 Issue: 13 Pages: 5137-5139
  • DOI: 10.1021/ic070289m
  • Peer Reviewed
• [Journal Article] Hydrogen peroxide dependent monooxygenations by tricking the substrate recognition of cytochrome P450_<BSβ> (2007)
  • Author(s): 荘司長三, 藤城貴史, 中島洋, 金美沙, 永野真吾, 城宜嗣, 渡辺芳人
  • Journal Title: Angew.Chem.Int.Ed. 46 Pages: 3656-3659
  • Peer Reviewed
• [Journal Article] Hydrogen Peroxide Dependent Monooxygenations by Tricking the Substrate Recognition of Cytochrome P450_<BSβ> (2007)
  • Author(s): O. Shoji, T. Fujishiro, H. Nakajima, M. Kim, S. Nagano, Y. Shiro, and Y. Wata-nabe,
  • Journal Title: Angew. Chem. Int. Ed. 46 Pages: 3656-3659
  • Peer Reviewed
• [Journal Article] Design and Structure Analysis of Artificial Metalloproteins: Selective Coordinati-on of His64 to Copper Complexes with Square-Planar Structure in the apo-Myo-globin Scaffold (2007)
  • Author(s): S. Abe, T. Ueno, P.A.N. Reddy, S. Okazaki, T. Hikage, A. Suzuki, T. Yamane, H. Nakajima, and Y. Wat-anabe
  • Journal Title: Inorg. Chem. 46 Pages: 5137-5139
  • Peer Reviewed
• [Journal Article] Incorporation of a Phebox Rhodium Complex into apo-Myoglobin Affords a Stable Organometallic Protein Showing Unprecedented Arrangement of the Complex in the Cavity (2007)
  • Author(s): Y. Satake, S. Abe, S. Okazaki, N. Ban, T. Hikage, T. Ueno, H. Nakajima, A. Suzuki, T. Yamane, H. Nis-hiyama, and Y. Watanabe
  • Journal Title: Organometallics 26 Pages: 4904-4908
  • Peer Reviewed
• [Journal Article] Reactivities of Oxo and peroxo Intermediates Studied by Hemoprotein Mutants (2007)
  • Author(s): Y. Watanabe, H. Nakajima, and T. Ueno
  • Journal Title: Acc. Chem. Res. 40 Pages: 554-562
  • Peer Reviewed
• [Journal Article] Coordination Design of Artificial Metalloproteins Utilizing Protein Vacant Space (2007)
  • Author(s): T. Ueno, S. Abe, N. Yokoi, and Y. Watanabe
  • Journal Title: Coord. Chem. Rev. 251 Pages: 2717-2731
  • Peer Reviewed
• [Journal Article] Use of Perfluoro Carboxylic Acids to Trick Cytochrome P450BM3 into Initiating Hydroxylation of Gaseous Alkanes
  • Author(s): N.Kawakami, O.Shoji, Y.Watanabe
  • Journal Title: Angew.Chem.Int.Ed. Volume: (印刷中,未定)
  • Peer Reviewed
• [Journal Article] Molecular Design of Heme Proteins for Future Application
  • Author(s): H.Nakajima, O.Shoji, Y.Watanabe
  • Journal Title: Catalysis Surveys from Asia
  • Peer Reviewed
• [Presentation] Construction of Metalloenzymes : Catalysts Embedded in Proteins (2012)
  • Author(s): Y.Watanabe
  • Organizer: Green & Sustainable Chemistry for Next Generation-The 5th GCOE International Symposium-(Keynote lecture)
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2012-02-22
• [Presentation] Construction of Metalloenzymes : Catalysts Embedded in Proteins (2012)
  • Author(s): Y. Watanabe
  • Organizer: Green & Sustainable Chemistry for Next Generation-The 5th GCOE International Symposium "Catalysis as the Basis for the Innovation in Materials Science" The 1st International Symposium of Graduate School of Chemical Sciences and Engineering
  • Place of Presentation: Sapporo, Japan
• [Presentation] Peroxide Dependent Monooxygenation Catalyzed by Cytochromes P450 (2011)
  • Author(s): Y.Watanabe, O.Shoji, N.Kawakami, T.Fujishiro
  • Organizer: 3rd Asian Conference on Coordination Chemistry (Keynote lecture)
  • Place of Presentation: India Habitat Centre (New Delhi, India)
  • Year and Date: 2011-10-17
• [Presentation] Novel Approaches for the Construction of Metalloenzymes (2011)
  • Author(s): Y.Watanabe
  • Organizer: The 12th joint IRTG SYMPOSIUM between Nagoya University and University of Munster
  • Place of Presentation: 名古屋大学(名古屋)(Invited Lecture)
  • Year and Date: 2011-10-04
• [Presentation] 蛋白質内部空間の化学:ヘム蛋白質から有機金属酵素へ (2011)
  • Author(s): 渡辺芳人
  • Organizer: 錯体化学会第61回討論会
  • Place of Presentation: 岡山理科大学(岡山)(受賞講演)
  • Year and Date: 2011-09-19
• [Presentation] Four Approaches for the Construction of Metalloenzymes (2011)
  • Author(s): Y.Watanabe
  • Organizer: ICBIC15 (Keynote lecture)
  • Place of Presentation: University of British Columbia (Vancouver, Canada)
  • Year and Date: 2011-08-09
• [Presentation] Construction of Peroxide Dependent Monooxygenase System (2011)
  • Author(s): Y.Watanabe
  • Organizer: France-Japan Coordination Chemistry Symposium
  • Place of Presentation: the Economy Faculty of the University of Rennes 1, the Amphitheater Donzelot of the University of Rennes 1 (Rennes, France)(Invited Lecture)
  • Year and Date: 2011-07-01
• [Presentation] 金属酵素機能創成:三つのアプローチ (2011)
  • Author(s): 渡辺芳人
  • Organizer: 日本化学会第91春季年会(2011)
  • Place of Presentation: 日本化学会第91春季年会(2011)講演予稿集
  • Year and Date: 2011-03-11
• [Presentation] Peroxide Dependent Monooxygenation Catalyzed by Cytochromes P450 (2011)
  • Author(s): Y. Watanabe, O. Shoji, N. Kawakami, T. Fujishiro
  • Organizer: 3rd Asian Conference onCoordination Chemistry
  • Place of Presentation: New Delhi, India
• [Presentation] Four Approaches for the Construction of Metalloenzymes (2011)
  • Author(s): Y. Watanabe
  • Organizer: ICBIC15
  • Place of Presentation: Vancouver, Canada
• [Presentation] 蛋白質空間を利用する金属触媒反応の開発 (2010)
  • Author(s): 渡辺芳人
  • Organizer: 神奈川大学シンポジウム-生命現象に学ぶ機能物質の創製-
  • Place of Presentation: 神奈川大学横浜キャンパス(神奈川県横浜市)
  • Year and Date: 2010-10-02
• [Presentation] Hydrogen Peroxide-Dependent Cytochrome P450 (2010)
  • Author(s): Y. Watanabe
  • Organizer: The 5th Asian Bioinorganic Chemistry Conference(AsBIC-V)
  • Place of Presentation: Kaohsiung, Taiwan
• [Presentation] Molecular Design of Catalytic reactions in Proteins (2010)
  • Author(s): Y. Watanabe
  • Organizer: 6th Tokyo Conference and Advanced Catalytic Science and Technology & 5th Asai Pcific Congress on Catalysis(TOCAT6/APCAT5)
  • Place of Presentation: Sapporo, Japan
• [Presentation] Construction of Organometalloenzymes (2010)
  • Author(s): Y. Watanabe
  • Organizer: 5th International Symposium on Bioorganometallic Chemistry(ISBOMC5)
  • Place of Presentation: Bochum, Germany
• [Presentation] Coordination Chemistry in Protein Cages (2010)
  • Author(s): Y. Watanabe
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies(Pacifichem)
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] Coordination Chemistry in Protein Cages (2010)
  • Author(s): Y. Watanabe
  • Organizer: 10th European Biological Inorganic Chemistry Conference(Eurobic10)
  • Place of Presentation: Thessaloniki, Greece
• [Presentation] Coordination Chemistry in Protein Cages (2010)
  • Author(s): Y.Watanabe
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem)
  • Place of Presentation: ハワイコンベンションセンター(Honolulu, Hawaii, USA)
• [Presentation] Hydrogen Peroxide-Dependent Cytochrome P450 (2010)
  • Author(s): Y.Watanabe
  • Organizer: The 5th Asian Bioinorganic Chemistry Conference (AsBIC-V)
  • Place of Presentation: The Ambassador Hotel (Kaohsiung, Taiwan)
• [Presentation] Design of Organometalloenzymes (2010)
  • Author(s): Y.Watanabe
  • Organizer: 3rd EuCheMS Chemistry Congress Ntimberg 2010
  • Place of Presentation: NUrnberg Messe GmbH (Nurnberg, Germany)
• [Presentation] Molecular design of catalytic reactions in proteins (2010)
  • Author(s): Y.Watanabe
  • Organizer: The Sixth Tokyo Conference on Advanced Catalytic Science and Technology (TOCAT6) and The Fifth Asia Pacific Congress on Catalysis (APCAT5)
  • Place of Presentation: 札幌コンベンションセンター(北海道札幌市)
• [Presentation] Construction of Organometalloenzymes (2010)
  • Author(s): Y.Watanabe
  • Organizer: The 5th International Symposium on Bioorganometallic Chemistry (ISBOMC'10)
  • Place of Presentation: Ruhr-Universitat Bochum (Bochum, Germany)
• [Presentation] Coordination Chemistry in Protein Cages (2010)
  • Author(s): Y.Watanabe
  • Organizer: 10th European Biological Inorganic Chemistry Conference (Eurobic 10)
  • Place of Presentation: Aristotle University of Thessaloniki (Thessaloniki, Greece)
• [Presentation] 蛋白ケージの中の化学:金属酵素の設計 (2010)
  • Author(s): 渡辺芳人
  • Organizer: 第37回生体分子科学討論会
  • Place of Presentation: 山口大学吉田キャンパス(山口県山口市)
• [Presentation] Coordination Chemistry in Proteins : Construction of Organomatalloproteins (2010)
  • Author(s): Y.Watanabe
  • Organizer: The 3rd International Conference on Joint Project of Chemical Synthesis Core Research Institutions
  • Place of Presentation: Nagoya, Japan
• [Presentation] Coordination Chemistry in Protein Cages (2010)
  • Author(s): Y.Watanabe
  • Organizer: The Metals In Biology Gordon Research Conference
  • Place of Presentation: Ventura, USA
• [Presentation] Inorganic Chemistry in Proteins (2009)
  • Author(s): Y. Watanabe
  • Organizer: Symposium on Modern Trends in Inorganic Chemistry :(MTIC XIII)
  • Place of Presentation: Bangalore, India
• [Presentation] Hydrogen Peroxide-Dependent Cytochrome P450 from Bacillus Subtilis(P450BSβ) (2009)
  • Author(s): Y. Watanabe
  • Organizer: Symposium on Advanced Biological Inorganic Chemistry(SABIC-2009)
  • Place of Presentation: Mumbai, India
• [Presentation] Coordination Chemistry in Protein Cages : Construction of Metalloproteins (2009)
  • Author(s): Y. Watanabe
  • Organizer: 2nd Asian Conference on Coordination Chemistry(2nd ACCC 2009)
  • Place of Presentation: Nanjing, China
• [Presentation] Hydrogen Peroxide-Dependent Cytochrome P450 from Bacillus Subtilis (P450BSβ) (2009)
  • Author(s): Y.Watanabe
  • Organizer: Symposium on Advanced Biological Inorganic Chemistry (SABIC-2009)
  • Place of Presentation: Mumbai, India
• [Presentation] Inorganic chemistry in proteins (2009)
  • Author(s): Y.Watanabe
  • Organizer: Modern Trends in Inorganic Chemistry (MTIC-XIII)
  • Place of Presentation: Bangalore, India
• [Presentation] Organometallo-Ferritins : A New Approach for Bioinorganic Chemistry (2009)
  • Author(s): Y.Watanabe
  • Organizer: 2nd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound, Canada
• [Presentation] Coordination Chemistry in Protein Cages : Construction of Metalloproteins (2009)
  • Author(s): Y.Watanabe
  • Organizer: 2nd Asian Conference on Coordination Chemistry (2^<nd>ACCC 2009)
  • Place of Presentation: Nanjing, China
• [Presentation] Molecular Design of Organometalloproteins (2009)
  • Author(s): Y. Watanabe
  • Organizer: The 1st G-COE International Symposium on Elucidation and Design of Materials and Molecular Functions
  • Place of Presentation: Nagoya, Japan
• [Presentation] 有機金属酵素の創製 (2008)
  • Author(s): 渡辺芳人
  • Organizer: 近畿化学協会有機金属部会第2回(東京)例会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-25
• [Presentation] Molecular Design of Organometalloprotein (2008)
  • Author(s): Y. Watanabe
  • Organizer: IISc-Nagoya University Joint Science and Technology Seminar : From Nanoscience to Atmospheric Science
  • Place of Presentation: Bangalore, India
  • Year and Date: 2008-06-16
• [Presentation] 有機金属酵素の分子設計:蛋白質内部空間への金属錯体、金属イオンの導入 (2008)
  • Author(s): 渡辺芳人
  • Organizer: 第2期菅野シンポジウム(第4回講演会)
  • Place of Presentation: 東京
  • Year and Date: 2008-03-08
• [Presentation] Coordination Chemistry in Protein Cages (2008)
  • Author(s): Y. Watanabe
  • Organizer: Inorganic Chemistry Colloquium at Yale University
  • Place of Presentation: New Haven, USA
  • Year and Date: 2008-02-26
• [Presentation] Hydrogen peroxide-dependent monooxygenations of non-natural substrates induced by a substrate-misrecognition of cytochrome P450_<BSβ> (2008)
  • Author(s): 渡辺芳人, 荘司長三, 藤城貴史, 中島洋
  • Organizer: International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: ロシア (モスクワ)
• [Presentation] Coordination Chemistry on Protein Super Structures (2008)
  • Author(s): Y. Watanabe
  • Organizer: The 5th joint IRTG SYMPOSIUM between Nagoya University and University of Mtinster
  • Place of Presentation: Munster, Germany
• [Presentation] Hydrogen Peroxide-Dependent Monooxygenations of Non-Natural Substrates Induced by a Substrate-Misrecognition of Cytochrome P450_<BSβ> (2008)
  • Author(s): Y. Watanabe, O. Shoji, T. Fujishiro, H. Nakajima
  • Organizer: International Conference on Porphyrins and Phthalocyanines
  • Place of Presentation: Moscow, Russia
• [Presentation] Construction of Organometalloproteins : A New Approach for Bioinorganic Chemistry (2008)
  • Author(s): Y. Watanabe
  • Organizer: The 4th Asian Biological Inoreaic Chemistry Conference
  • Place of Presentation: Cheju, Korea
• [Presentation] Proteins are Great Playgrounds of Coordination Chemistry (2008)
  • Author(s): 渡辺芳人
  • Organizer: 第18回金属の関与する生体関連反応シンポジウム(SRM2008)
  • Place of Presentation: 名古屋
• [Presentation] Coordination Chemistry in Protein Cages (2008)
  • Author(s): Y. Watanabe, T. Ueno, T. Koshiya-ma, M. Suzuki, S. Abe, and M Abe
  • Organizer: The 10th Eurasia Conference on Chemical Sciences (EuAs C_2S-10)
  • Place of Presentation: Manila, Phillipines
• [Presentation] 生体反応場錯体化学 (2008)
  • Author(s): 渡辺芳人
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] ナノサイズ空間を有する蛋白質を利用した金属微粒子作成 (2008)
  • Author(s): 渡辺芳人
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 東京
• [Presentation] Construction of Organometalloproteins (2007)
  • Author(s): Y. Watanabe
  • Organizer: The Second International Conference on Joint Project of Chemical Synthesis Core Research Institutions
  • Place of Presentation: Kyoto, Japan.
  • Year and Date: 2007-08-07
• [Presentation] Construction of Organometalloproteins : A New Approach for Bioinorganic Chemistry (2007)
  • Author(s): Y. Watanabe
  • Organizer: 13th International Conference on Inorganic Biological Chemistry(ICBIC 13)
  • Place of Presentation: Vienna, Austria
• [Presentation] Construction of Organometalloproteins: A New Approach for Bioinorganic Chemistry (2007)
  • Author(s): Y. Watanabe
  • Organizer: Japan-USA Joint Symposium of Chemistry of Coordination Space
  • Place of Presentation: Evanston, USA
• [Presentation] Construction of Organometalloproteins: A New Approach for Bioinorganic Chemistry (2007)
  • Author(s): Y. Watanabe
  • Organizer: The First Asian Conference on Coordination Chemistry
  • Place of Presentation: Okazaki, Japan
• [Presentation] Transformation of Cytochrome P450_<BSβ> into a Versatile Peroxygenase (2007)
  • Author(s): Y. Watanabe
  • Organizer: The 4th joint IRTG SYMPOSIUM between Nagoya University and University of Munster
  • Place of Presentation: Munster, Germany
• [Presentation] Introduction of Metal Cofactors in Protein cages: Construction of Organometallo protein (2007)
  • Author(s): Y. Watanabe
  • Organizer: The 67th Okazaki Conference
  • Place of Presentation: Okazaki, Japan
• [Presentation] Molecular Design of Organometalloenzymes (2007)
  • Author(s): Y. Watanabe
  • Organizer: The 11th International Chemical Conference, Taipei (ICCT)
  • Place of Presentation: Hsinchu, Taiwan
• [Book] 「P450による酸素活性化機構と基質の酸素化機構」、P450の分子生物学 (2009)
  • Author(s): 渡辺芳人、荘司長三
  • Publisher: 講談社サイエンティフィク
• [Book] ヘム酵素の創成 (2009)
  • Author(s): 渡辺芳人、荘司長三
  • Publisher: 酵素工学ニュース
• [Book] “蛋白質が提供するナノ空間”ナノ空間材料の創製と応用 (2009)
  • Author(s): 渡辺芳人、安部聡、上野隆史
  • Publisher: フロンティア出版
• [Book] “蛋白空間錯体hybrid.”配位空間の化学 (2009)
  • Author(s): 渡辺芳人、上野隆史
  • Publisher: シーエムシー出版
• [Book] 「P450による酸素活性化機構と基質の酸素化機構」P450の分子生物学(第2版) (2009)
  • Author(s): 渡辺芳人, 荘司長三
  • Total Pages: 297
  • Publisher: 講談社サイエンティフィク
• [Book] 「酵素反応の速度と反応機構」、触媒便覧第4章 (2008)
  • Author(s): 渡辺芳人、中島洋
  • Publisher: 講談社サイエンティフィック
• [Book] 好熱菌由来タンパク質を化学の視点で利用する (2008)
  • Author(s): 中島洋、渡辺芳人
  • Publisher: 学術月報
• [Book] 有機金属酵素創製の試み (2008)
  • Author(s): 渡辺芳人
  • Publisher: Organo Metallic NEWS
• [Remarks]
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/
• [Remarks]
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/
• [Remarks] ホームページ
  • URL: http://bioinorg.chem.nagoya-u.ac.jp
• [Remarks]
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/
• [Remarks]
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/
• [Remarks]
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/
• [Patent(Industrial Property Rights)] ヘム蛋白質のヘム置換法 (2012)
  • Inventor(s): 渡辺芳人、荘司長三、川上了史
  • Industrial Property Rights Holder: 名古屋大学
  • Filing Date: 2012-03-13
  • Overseas
• [Patent(Industrial Property Rights)] ヘム蛋白質のヘム置換法 (2012)
  • Inventor(s): 渡辺芳人、荘司長三、川上了史
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Filing Date: 2012-03-13
  • Overseas
• [Patent(Industrial Property Rights)] 不活性炭化水素の水酸化方法およびダミー分子 (2010)
  • Inventor(s): 渡辺芳人、荘司長三、川上了史
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 2010-164828
  • Filing Date: 2010-07-22