Osmoregulatory MAP kinase signal transduction pathway

• Project/Area Number: 19107004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SAITO Haruo 東京大学, 医科学研究所, 教授 (60114485)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥106,340,000 (Direct Cost: ¥81,800,000、Indirect Cost: ¥24,540,000); Fiscal Year 2011: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2010: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2009: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2008: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2007: ¥28,340,000 (Direct Cost: ¥21,800,000、Indirect Cost: ¥6,540,000)
• Keywords: 細胞内シグナル伝達 / ストレス応答 / MAPキナーゼ / 浸透圧センサー / 酵母

Research Abstract

When exposed to environmental hyperosmolarity, the budding yeast Saccharomyces cerevisiae activates the Hog1 MAP kinase signal pathway, which governs an array of adaptive responses. In this project, we investigated how the activity of the Hog1 MAPK pathway is regulated by the two newly discovered osmosensors Hkr1 and Msb2. We also investigated the roles of the membrane anchor protein Opy2, membrane protein Sho1, and the adaptor protein Ste50 in dynamic regulation of the Hog1 MAPK pathway.

Research Products

• [Journal Article] Oncogenic Ras abrogates MEK SUMOylation that suppresses the ERK pathway and cell transformation (2011)
  • Author(s): Kubota Y, O' Grady P, Saito H, and Takekawa M
  • Journal Title: Nature Cell Biol Volume: 13 Pages: 282-291
  • Peer Reviewed
• [Journal Article] Oncogenic Ras abrogates MEK SUMOylation that suppresses the ERK pathway and cell transformation (2011)
  • Author(s): Yuji Kubota
  • Journal Title: Nature Cell Biology Volume: 13 Pages: 282-291
  • Peer Reviewed
• [Journal Article] Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor (2010)
  • Author(s): Yamamoto K, Tatebayashi K, Tanaka K, and Saito H
  • Journal Title: Molecular Cell Volume: 40 Pages: 87-98
  • Peer Reviewed
• [Journal Article] Regulation of cross-talk inyeast MAPK signaling pathways (2010)
  • Author(s): Saito H
  • Journal Title: Curr Opin Microbiol Volume: 13 Pages: 677-683
  • Peer Reviewed
• [Journal Article] Regulation of cross-talk in yeast MAPK signaling pathways (2010)
  • Author(s): Haruo Saito
  • Journal Title: Current Opinion in Microbiology Volume: 13 Pages: 677-683
  • Peer Reviewed
• [Journal Article] Stimulus-specific distinctions in spatial and emporal dynamics of stressactivated protein kinase kinase kinases revealed by a fluorescence resonance energy transfer biosensor (2009)
  • Author(s): Tomida T, Takekawa M, O' Grady P, and Saito H
  • Journal Title: Mol. Cell. Biol Volume: 29 Pages: 6117-6127
  • Peer Reviewed
• [Journal Article] Glycosylation defects activate filamentous growthKss1 MAPK and inhibit osmoregulatory Hog1 MAPK (2009)
  • Author(s): Yang HY, Tatebayashi K, Yamamoto K, and Saito H
  • Journal Title: EMBO J Volume: 28 Pages: 1380-1391
  • Peer Reviewed
• [Journal Article] Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK (2009)
  • Author(s): Yang, H.-Y., Tatebayashi, K., Yamamoto, K., Saito, H.
  • Journal Title: EMBO J. 28 Pages: 1380-1391
  • Peer Reviewed
• [Journal Article] Stimulus-specific distinctions in spatial and temporal dynamics of stress-activated protein kinase kinase kinases revealed by a fluorescence response energy transfer biosensor (2009)
  • Author(s): Tomida, T., Takekawa, M., O'Grady, P., Saito, H.
  • Journal Title: Mol.Cell.Biol. 29 Pages: 6117-6127
  • Peer Reviewed
• [Journal Article] Stimulus-specific distinctions in spatial and temporal dynamics of stress-activated protein kinase kinase kinases revealed by a fluorescence response energy transfer biosensor. (2009)
  • Author(s): Taichiro Tomida
  • Journal Title: Molecular and Cellular Biology 29 Pages: 6117-6127
  • Peer Reviewed
• [Journal Article] Glycosylation defects activate filamentous growth Kssl MAPK and inhibit osmoregulatory Hogl MAPK. (2009)
  • Author(s): Yang Hui-Yu
  • Journal Title: The EMBO Journal 28 Pages: 1380-1391
  • Peer Reviewed
• [Journal Article] Formation of stress granules inhibits apoptosis by suppressing stress-responsive MAPK pathways (2008)
  • Author(s): Arimoto K, Fukuda H, Imajo-Ohmi S, Saito H, and Takekawa M
  • Journal Title: Nature Cell Biol Volume: 10 Pages: 1324-1332
  • Peer Reviewed
• [Journal Article] Phosphorylated Ssk1 prevents unphosphorylated Ssk1 from activating the Ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway (2008)
  • Author(s): Horie T, Tatebayashi K, Yamada R, and Saito H
  • Journal Title: Mol. Cell. Biol Volume: 28 Pages: 5172-5183
  • Peer Reviewed
• [Journal Article] Two adjacent docing sites in the yeast Hog1 mitogen-activated protein(MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase (2008)
  • Author(s): Murakami Y, Tatebayashi K, and Saito H
  • Journal Title: Mol. Cell. Biol Volume: 28 Pages: 2481-2494
  • Peer Reviewed
• [Journal Article] Formation of stress granules inhibits apoptosis by suppressing stress-responsive MAPK pathways (2008)
  • Author(s): Arimoto, K., Fukuda, H., Imajo-Ohmi, S., Saito, H., Takekawa, M.
  • Journal Title: Nature Cell Biol. 10 Pages: 1324-1332
  • Peer Reviewed
• [Journal Article] Two adjacent docking sites in the yeast Hogl mitogen-activated protein (MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyroslne phosphatase. (2008)
  • Author(s): Yulia Murakami
  • Journal Title: Molecular and Cellular Biology 28 Pages: 2481-2494
  • Peer Reviewed
• [Journal Article] Phosphorylated Sskl prevents unphosphorylated Sskl from activating the Ssk2 mitogen-activated protein kimase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway. (2008)
  • Author(s): Tetsuro Horie
  • Journal Title: Molecular and Cellular Biology 1 28 Pages: 5172-5183
  • Peer Reviewed
• [Journal Article] Formation of stress granules inhibits apoptosis by suppressing stress-responsive MAPK pathways. (2008)
  • Author(s): Kyoko Arimoto
  • Journal Title: Nature Cell Biology 10 Pages: 1324-1332
  • Peer Reviewed
• [Journal Article] Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway (2007)
  • Author(s): Tatebayashi K, Tanaka K, Yang HY, Yamamoto K, Matsushita Y, Tomida T, Imai M, and Saito H
  • Journal Title: EMB J Volume: 26 Pages: 3521-3533
  • Peer Reviewed
• [Journal Article] Activation of MTK1/MEKK4 by GADD45 through induced N-C dissociation and dimerization-mediated trans autophosphorylation of the MTK1 kianse domain (2007)
  • Author(s): Miyake Z, Takekawa M., Ge Q, andSaito H
  • Journal Title: Mol. Cell. Biol Volume: 27 Pages: 2765-2776
  • Peer Reviewed
• [Journal Article] Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway (2007)
  • Author(s): Tatebayashi, K., Tanaka, K., Yang, H.-Y., Yamamoto, K., Matsushita, Y., Tomida, T., Imai, M., Saito, H.
  • Journal Title: EMBO J. 26 Pages: 3521-3533
  • Peer Reviewed
• [Journal Article] Two adjacent docking sites in the yeast Hogl mitogen-activated protein(MAP) kinase differentially interact with the Pbs2 MAP kinase kinase and the Ptp2 protein tyrosine phosphatase (2007)
  • Author(s): Murakami, Yulia
  • Journal Title: Molecular and Cellular Biology 28 Pages: 2481-2494
  • Peer Reviewed
• [Journal Article] Transmembrane mucins Hkrl and Msb2 are putative osmosensors in the Shol branch of yeast HOG pathway (2007)
  • Author(s): Tatebayashi, Kazuo
  • Journal Title: The EMBO Journal 26 Pages: 3521-3522
  • Peer Reviewed
• [Journal Article] Response to hyperosmotic stress
  • Author(s): Saito H, and Posas F
  • Journal Title: Genetics Volume: (in press)
  • Peer Reviewed
• [Presentation] Dynamic regulation of hyperosmotic stress response in the budding yeast (2011)
  • Author(s): 斎藤春雄
  • Organizer: Society of General Microbiology Conference
  • Place of Presentation: Harrogate, UK
  • Year and Date: 2011-04-13
• [Presentation] Dynamic regulation of hyperosmotic stress response in the budding yeast (2011)
  • Author(s): Haruo Saito
  • Organizer: Society for General Microbiology Conference 2011
  • Place of Presentation: Harrogate, UK
  • Year and Date: 2011-04-13
• [Presentation] Signal transduction pathways (2010)
  • Author(s): 斎藤春雄
  • Organizer: Analysis and Engineering of Biomolecular Systems
  • Place of Presentation: Spetses, Greece
  • Year and Date: 2010-09-14
• [Presentation] Osmosensory signal transduction inbudding yeast (2010)
  • Author(s): 斎藤春雄
  • Organizer: Gordon Research Conference on "Sensory Transduction in Microorganisms."
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 2010-01-27
• [Presentation] Gordon Research Conference on "Sensory Transduction in Microorganisms." (2010)
  • Organizer: Ventura
  • Place of Presentation: California, USA
  • Year and Date: 2010-01-27
• [Presentation] Osmosensory signal transduction in budding yeast (2010)
  • Author(s): Haruo Saito
  • Organizer: Gordon Research Conference on"Sensory Transduction in Microorganims."
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 2010-01-27
• [Presentation] Regulation of the osmoadaptive HOG MAPK pathway in the budding yeast (2009)
  • Author(s): 斎藤春雄
  • Organizer: Marie Curie Research & Training Network Meeting on "MAPK Signalling during Fungal Pathogenesis"
  • Place of Presentation: Berlin, Germa
  • Year and Date: 2009-10-28
• [Presentation] Regulation of the osmoadaptive HOG MAPK pathway in the budding yeast (2009)
  • Author(s): Haruo Saito
  • Organizer: Marie Curie Research & Training Network Meeting on"MAPK Signalling during Fungal Pathogenesis."
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2009-10-28
• [Presentation] Regulation of stress MAPK pathways in mammalian and yeast cells (2008)
  • Author(s): 斎藤春雄
  • Organizer: OIST国際ワークショップ
  • Place of Presentation: 沖縄県国頭郡恩納
  • Year and Date: 2008-03-10
• [Presentation] Regulation of stress MAPK pathways in mammalian and yeastcells (2008)
  • Author(s): 斎藤 春雄
  • Organizer: OIST(沖縄科学技術研究基盤整備機構)国際ワークショップ「Systems Biology on MAPK Pathways Workshop」
  • Place of Presentation: 沖縄県国頭郡恩納
  • Year and Date: 2008-03-10
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