Visualization of oscillation for stress signal and molecular genetic study of establishment of cell community

• Project/Area Number: 19109003
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: MIURA Masayuki 東京大学, 大学院・薬学系研究科, 教授 (50202338)
• Project Period (FY): 2007 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥109,720,000 (Direct Cost: ¥84,400,000、Indirect Cost: ¥25,320,000); Fiscal Year 2011: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000); Fiscal Year 2010: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000); Fiscal Year 2009: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000); Fiscal Year 2008: ¥21,580,000 (Direct Cost: ¥16,600,000、Indirect Cost: ¥4,980,000); Fiscal Year 2007: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
• Keywords: 発生 / 細胞社会 / カスパーゼ / 分化 / ショウジョウバエ / マウス / イメージンク / 神経発生 / 非対称分裂 / イメージング

Research Abstract

During animal development, differentiating cells face to trial and error process and acquire both intrinsic and extrinsic stress. Caspases are activated as one of stress responses. In this study, we established in vivo imaging system to monitor caspase activation. Live imaging of cell death and caspase activation allowed us to document the role of apoptosis signaling to establish the cellular communities during development.

Research Products

• [Journal Article] Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure (2011)
  • Author(s): Yamaguchi, Y., Shinotsuka, N., Nonomura, K., Takemoto, K, Kuida, K., Yoshida, H., Miura, M.
  • Journal Title: Journal of Cell Biology Volume: 195 Issue: 6 Pages: 1047-1060
  • DOI: 10.1083/jcb.201104057
  • Peer Reviewed
• [Journal Article] Conserved metabolic energy production pathways govern Eiger/TNF-induced non-apoptotic cell death (2011)
  • Author(s): Kanda, H., Igaki, T., Okano, H., and Miura, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Issue: 47 Pages: 18977-18982
  • DOI: 10.1073/pnas.1103242108
  • Peer Reviewed
• [Journal Article] Non-autonomusapoptosis is triggered by local cell cycl eprogression during epithelial replacement in Drosophila (2011)
  • Author(s): Nakajima, Y-I, Kuranaga, E., Sugimura, K., Miyawaki, A., and Miura, M.
  • Journal Title: Mol. Cell Biol Volume: 31 Issue: 12 Pages: 2499-2512
  • DOI: 10.1128/mcb.01046-10
  • Peer Reviewed
• [Journal Article] Apoptosis controls the speed of looping morphogenesis in Drosophila male terminalia (2011)
  • Author(s): Kuranaga, E., Matsunuma, T., Kanuka, H., Takemoto, K., Koto, A., Kimura, K and Miura, M.
  • Journal Title: Development Volume: 138 Issue: 8 Pages: 1493-1499
  • DOI: 10.1242/dev.058958
  • Peer Reviewed
• [Journal Article] Aging causes distinct characteristics of polyglutamine amyloids in vivo (2011)
  • Author(s): Tonoki, A., E. Kuranaga, N. Ito, Y. Nekooki-Machida, Tanaka, M., and Miura, M.
  • Journal Title: Genes to Cells Volume: 16 Issue: 5 Pages: 557-564
  • DOI: 10.1111/j.1365-2443.2011.01505.x
  • Peer Reviewed
• [Journal Article] Apoptosis ensures spacing pattern formation of Drosophila sensory organs (2011)
  • Author(s): Koto, A., Kuranaga, E., and Miura, M.
  • Journal Title: Current Biol Volume: 21 Issue: 4 Pages: 278-287
  • DOI: 10.1016/j.cub.2011.01.015
  • Peer Reviewed
• [Journal Article] Who lives and who dies : role of apoptosis in quashing developmental errors (2011)
  • Author(s): Koto, A., and Miura, M.
  • Journal Title: Comm. Integ. Biol Volume: 4 Pages: 495-497
• [Journal Article] Apoptotic and non-apoptotic caspase functions in neural development (2011)
  • Author(s): Miura, M.
  • Journal Title: Neurochemical Res Volume: 36 Issue: 7 Pages: 1253-1260
  • DOI: 10.1007/s11064-010-0341-x
• [Journal Article] Active participation of cell death in development and organismal homeostasis (2011)
  • Author(s): Miura, M
  • Journal Title: Dev. Growth. Diff Volume: 53 Issue: 2 Pages: 125-136
  • DOI: 10.1111/j.1440-169x.2010.01228.x
  • NAID: 10027899570
• [Journal Article] Apoptosis ensures spacing pattern formation of Drosophila sensory organs. (2011)
  • Author(s): Koto, A., Kuranaga, E., Miura, M.
  • Journal Title: Current Biol. Volume: 21 Pages: 278-287
  • Peer Reviewed
• [Journal Article] Maturation of the olfactory sensory neurons by Apaf-1/caspase-9-mediated caspase activity (2010)
  • Author(s): Ohsawa, S., Hamada, S., Kuida, K., Yoshida, H., Igaki, T., and Miura, M.
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 107 Issue: 30 Pages: 13366-13371
  • DOI: 10.1073/pnas.0910488107
• [Journal Article] Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon mediated gene transfer system (2010)
  • Author(s): Yoshida, A., Yamaguchi, Y., Nonomura, K., Kawakami, K., Takahashi, Y., and Miura, M
  • Journal Title: Genes to Cells Volume: 15 Issue: 5 Pages: 501-512
  • DOI: 10.1111/j.1365-2443.2010.01397.x
• [Journal Article] Simultaneous expression of different transgenes in neurons and glia by combining in utero electroporation with the Tol2 transposon mediated gene transfer system. (2010)
  • Author(s): Yoshida, A., Yamaguchi, Y., Nonomura, K., Kawakami, K., Takahashi, Y., Miura, M.
  • Journal Title: Genes to Cells Volume: 15 Pages: 501-512
  • Peer Reviewed
• [Journal Article] Maturation of the olfactory sensory neurons by Apaf-1/caspase-9-mediated caspase activity. (2010)
  • Author(s): Ohsawa, S., Hamada, S., Kuida, K., Yoshida, H., Igaki, T., Miura, .M.
  • Journal Title: Proc.Natl.Acad.Sci.USA. Volume: 107 Pages: 13366-13371
  • Peer Reviewed
• [Journal Article] Temporal regulation of Drosophila IAP determines the dual functions of caspases in sensory organ development (2009)
  • Author(s): Koto, A., Kuranaga, E., and Miura, M
  • Journal Title: J. Cell Biol Volume: 187 Issue: 2 Pages: 219-321
  • DOI: 10.1083/jcb.200905110
  • Peer Reviewed
• [Journal Article] Caspase-9 activation revealed by Semaphorin 7A cleavage is independent of apoptosis in the aged olfactory bulb (2009)
  • Author(s): Ohsawa, S., Hamada, S., Asou, H., Kuida, K., Uchiyama, Y., Yoshida, H., and Miura, M
  • Journal Title: J. Neurosci Volume: 29 Issue: 36 Pages: 11385-11392
  • DOI: 10.1523/jneurosci.4780-08.2009
• [Journal Article] Temporal regulation of Drosophila IAP determines the dual functions of caspases in sensory organ development (2009)
  • Author(s): Koto, A., Kuranaga, E., Miura, M.
  • Journal Title: J.Cell Biol. 187 Pages: 219-321
• [Journal Article] Caspase-9 activation revealed by Semaphorin 7A cleavage is independent of apoptosis in the aged olfactory bulb (2009)
  • Author(s): Ohsawa, S., Hamada, S., Asou, H., Kuida, K., Uchiyama, Y., Yoshida, H., Miura, M.
  • Journal Title: J.Neurosci. 29 Pages: 11385-11392
• [Journal Article] Intrincic tumor suppression and epithelial maintenance by endocytic activation of Eiger/TNF signaling in Drosophila. (2009)
  • Author(s): Igaki, T., Pastor-Pareja, J. C., Aonuma, H., Miura, M., and Xu, T
  • Journal Title: Dev. Cell 16 Pages: 458-465
  • Peer Reviewed
• [Journal Article] Genetic evidence linking age-dependent attenuation of the 26S proteasome with aging process. (2009)
  • Author(s): Tonoki, A., Kuranaga, E., Tomioka, T., Hamazaki, J., Murata, S., Tanaka, K., and Miura, M
  • Journal Title: Mol. Cell Biol 29 Pages: 1095-1106
  • Peer Reviewed
• [Journal Article] Caspase-mediated changes in Histone H1 in early apoptosis : prolonged caspase activation in developing olfactory sensory neurons (2008)
  • Author(s): Ohsawa, S., Hamada, S., Yoshida, H., and Miura, M
  • Journal Title: Cell Death Diff Volume: 15 Issue: 9 Pages: 1429-1439
  • DOI: 10.1038/cdd.2008.71
• [Journal Article] Caspase-mediated changes in Histone H1 in early apoptosis : prolonged caspase activation in developing olfactory sensory neurons (2008)
  • Author(s): Ohsawa, S., Hamada, S., Yoshida, H., Miura, M.
  • Journal Title: Cell Death Diff. 15 Pages: 1429-1439
• [Journal Article] RNA-binding protein Hoip accelerates polyQ-induced neurodegeneration in Drosophila. (2008)
  • Author(s): Murata, T., Suzuki, E., Ito, S., Sawatsubashi, S., Zhao, Y., Yamagata, K., Tanabe, M., Fujiyama, S., Kimura, S., Ueda, T., Matsukawa, H., KUranaga, E., Miura, M., Takeyama, K., and Kato, S.
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 2255-2261
  • NAID: 10027531576
  • Peer Reviewed
• [Journal Article] A Drosophila orphan GPCR BOSS functions as a glucose-responding receptor: loss of boss causes abnormal sugar and lipid metabolism. (2008)
  • Author(s): Kohyama-Koganeya, A., Kim, Y-J., Miura, M., and Hirabayashi, Y.
  • Journal Title: PNAS 105 Pages: 15328-15333
  • Peer Reviewed
• [Journal Article] Novel antibody to human BASPl labels apoptotic cells post-caspase activation (2008)
  • Author(s): Ohasawa. S., Watanabe, T., Katada, T., Nishina, H., and Miura, M.
  • Journal Title: BBRC 371 Pages: 639-643
  • Peer Reviewed
• [Journal Article] Caspase-mediated changes in Histone Hl in early apoptosis: prolonged caspase activation in developing olfactory sensory neurons. (2008)
  • Author(s): Ohsawa, S., Hamada, S., Yoshida, H., anda Miura, M.
  • Journal Title: Cell Death Diff 15 Pages: 1429-1439
  • Peer Reviewed
• [Journal Article] Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo (2007)
  • Author(s): Takemoto, K., Kuranaga, E., Tonoki, A., Nagai, T., Miyawaki, A., and Miura, M
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 104 Pages: 13367-13372
• [Journal Article] Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo (2007)
  • Author(s): Takemoto, K., Kuranaga, E., Tonoki, A., Nagai, T., Miyawaki, A., Miura, M.
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 13367-13372
• [Journal Article] Evolution of mitochondrial cell death pathway:Proapoptotic role of HtrA2/Omi in Drosophila (2007)
  • Author(s): Igaki, T., Suzuki, Y., Tokushige, N., Aonuma, H., Takahashi, R, and Miura, M
  • Journal Title: BBRC 356 Pages: 993-997
  • Peer Reviewed
• [Journal Article] Local initiation of caspase activation in Drosophila salivary gland programmed cell death in vivo (2007)
  • Author(s): Takemoto, K., Kuranaga, E., Tonoki, A., Nagai, T., Miyawaki, A., and Miura, M
  • Journal Title: Proc.Natl.Acad.Sci.USA 104 Pages: 13367-13372
  • Peer Reviewed
• [Journal Article] Tumor suppressor CYLD regulates JNK-Induced cell death in Drosophila (2007)
  • Author(s): Xue, L., Igaki, T., Kuranaga, E., Kanda, H., Miura, M., and Xu.T
  • Journal Title: Dev.Cell 13 Pages: 446-454
  • Peer Reviewed
• [Journal Article] DIAP2 functions as a mechanism-based regulator of drICE that conttributes to the caspase activity threshold in living cells (2007)
  • Author(s): Ribeiro, P.S., Kuranaga, E., Tenev, T., Leulier, F., Miura, M., Meier.P
  • Journal Title: J.Cell Biol 179 Pages: 1467-1480
  • Peer Reviewed
• [Presentation] Physiological function of caspases in organogenesis and tissue remodeling (2011)
  • Author(s): Miura, M
  • Organizer: 25^<th> Annual French Drosophila Conference
  • Place of Presentation: Lyon, France
• [Presentation] The replacement boundary coordinates epidermal remodeling in Drosophila (2011)
  • Author(s): Miura, M
  • Organizer: 第63回日本細胞生物学会シンポジウム
  • Place of Presentation: 札幌、日本
• [Presentation] Roles of programmed cell death signaling in Drosophila sensory organ development (2010)
  • Author(s): Miura, M.
  • Organizer: International Symposium on "Cell Cycle and Development"
  • Place of Presentation: Kyoto, Japan
• [Presentation] Caspase regulates axon pathfinding and maturation of mouse olfactory neurons (2010)
  • Author(s): Miura, M.
  • Organizer: International Symposium on Morphological Sciences
  • Place of Presentation: Taormina, Italy
• [Presentation] Caspase regulates axon pathfinding and maturation of mouse olfactory neurons. (2010)
  • Author(s): Miura, M
  • Organizer: International Symposium on Morphological Sciences.
  • Place of Presentation: Taormina, Italy
• [Presentation] TNF signaling and its physiological roles in Drosophila (2009)
  • Author(s): Miura, M
  • Organizer: 12^<th> International TNF conference
  • Place of Presentation: Madrid, Spain
• [Presentation] In vivo dynamics of cell death signaling during animal development. (2008)
  • Author(s): Miura, M.
  • Organizer: Symopsium in BNB 2008
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2008-12-09
• [Presentation] In vivo dynamics of cell death signaling in the Drosophila sensory organ development. (2008)
  • Author(s): Miura, M.
  • Organizer: International Cell Death Society Symposiu
  • Place of Presentation: Shanghai, China
  • Year and Date: 2008-06-07
• [Presentation] In vivo dynamics of cell death signaling in the Drosophila sensory organ development (2008)
  • Author(s): Miura, M.
  • Organizer: International Cell Death Society Symposium
  • Place of Presentation: Shanghai, China
• [Presentation] Non-apoptotic function of caspase signaling in Drosophila (2007)
  • Author(s): Miura, M
  • Organizer: Keystone Symposium on Apoptotic and Non-apoptotic Cell Death Pathways
  • Place of Presentation: Monterey, California, USA
• [Presentation] Non-apoptotic function of caspase signaling in Drosophila. (2007)
  • Author(s): Miura, M.
  • Organizer: Keystone Symposium
  • Place of Presentation: Monterey, California, USA
• [Presentation] Novel function of caspase activation signal in neural development. (2007)
  • Author(s): Miura, M.
  • Organizer: Symposium in BMB 2007
  • Place of Presentation: Yokohama, Japan.
• [Remarks]
  • URL: http://www.f.u-tokyo.ac.jp/~genetics/index.html
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