Mutagenesis using transposon system in C57BL/6 mice

• Project/Area Number: 19200034
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: TAKEDA Junji Osaka University, 先端科学イノベーションセンター, 教授 (50163407)
• Co-Investigator(Kenkyū-buntansha): HORIE Kyoji 大阪大学, 医学系研究科, 准教 (30333446)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000); Fiscal Year 2009: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2007: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: トランスポゾンシステム / ミュタゲネーシス / C57BL / 6マウス / 表現型解析 / 病態モデル / トランスポゾン / C57BL/6マウス

Research Abstract

We have succeeded generation of mutant mice with Sleeping Beauty transposon system. In this project, we established such system in C57BL/6 mice. To stably express SB transposase, we targeted the transposase gene into Rosa26 locus using C57BL/6 ES cells. We are able to distribute the ES cell line.

Research Products

• [Journal Article] SMOC1 is essential for ocular and limb development in humans and mice. (2011)
  • Author(s): Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H.
  • Journal Title: Am J Hum Genet. 88 Pages: 30-41
  • Peer Reviewed
• [Journal Article] Diacylglycerol kinase β knockout mice exhibit lithium-sensitive behavioral abnormalities. (2010)
  • Author(s): Kakefuda K, Oyagi A, Ishisaka M, Tsuruma K, Shimazawa M, Yokota K, Shirai Y, Horie K, Saito N, Takeda J, Hara H.
  • Journal Title: PLoS One. 5
  • Peer Reviewed
• [Journal Article] Functional genomics in the mouse using the sleeping beauty transposon system. (2010)
  • Author(s): Horie K, Kokubu C, Takeda J.
  • Journal Title: Methods Enzymol. 477 Pages: 71-89
  • Peer Reviewed
• [Journal Article] Essential role of neuron-enriched diacylglycerol kinase (DGK), DGKbeta in neurite spine formation, contributing to cognitive function. (2010)
  • Author(s): Shirai Y, Kouzuki T, Kakefuda K, Moriguchi S, Oyagi A, Horie K, Morita SY, Shimazawa M, Fukunaga K, Takeda J, Saito N, Hara H.
  • Journal Title: PLoS One. 5(7)
  • Peer Reviewed
• [Journal Article] Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro. (2010)
  • Author(s): Yagita K, Horie K, Koinuma S, Nakamura W, Yamanaka I, Urasaki A, Shigeyoshi Y, Kawakami K, Shimada S, Takeda J, Uchiyama Y.
  • Journal Title: Proc Natl Acad Sci USA. 107(8) Pages: 3846-3851
  • Peer Reviewed
• [Journal Article] A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in the mouse genome. (2009)
  • Author(s): Kokubu C, Horie K, Abe K, Ikeda R, Mizuno S, Uno Y, Ogiwara S, Ohtsuka M, Isotani A, Okabe M, Imai K, Takeda J
  • Journal Title: Nature Genet. 41 Pages: 946-952
  • Peer Reviewed
• [Journal Article] Generating mutant rats using the Sleeping Beauty transposon system. (2009)
  • Author(s): Kitada K, Keng VW, Takeda J, Horie K.
  • Journal Title: Methods. 49(3) Pages: 236-242
  • Peer Reviewed
• [Journal Article] Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon. (2009)
  • Author(s): Yusa K , Rad R, Takeda J, Bradley A
  • Journal Title: Nat.Methods 6 Pages: 363-369
  • Peer Reviewed
• [Journal Article] A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in the mouse genome. (2009)
  • Author(s): Kokubu C, et al
  • Journal Title: Nature Genet. Volume: 41 Pages: 946-52
  • Peer Reviewed
• [Journal Article] Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon. (2009)
  • Author(s): Yusa K, et al
  • Journal Title: Nat. Methods Volume: 6 Pages: 363-369
  • Peer Reviewed
• [Journal Article] Generating mutant rats using the Sleeping Beauty transposon system. (2009)
  • Author(s): Kitada K, et al
  • Journal Title: Methods. Volume: 49 Pages: 236-42
  • Peer Reviewed
• [Journal Article] Translation from nonautonomous type IAP retrotransposon is a critical determinant of transposition activity : implication for retrotransposon-mediated genome evolution. (2008)
  • Author(s): Saito ES, Keng VW, Takeda J, Horie K
  • Journal Title: Genome Res. 18(6) Pages: 859-868
  • Peer Reviewed
• [Journal Article] Insertional mutagenesis of the mouse germline with Sleeping Beauty transposition.Methods Mol Biol.: Chromosomal Mutagenesis (2008)
  • Author(s): Takeda J, Izsvak Z, Ivies Z
  • Journal Title: J.Kayser(Edited by Gregory D.Davis , Kevin) 435 Pages: 109-125
  • Peer Reviewed
• [Journal Article] T Cell Response Mediated by Myeloid Cell-Derived IL-12 Is Responsible for Porphyromonas gingivalis-Induced Periodontitis in IL-10-Deficient Mice. (2008)
  • Author(s): Sasaki H, Suzuki N, Kent R Jr, Kawashima N, Takeda J, Stashenko P.
  • Journal Title: J Immunol. 1;180 Pages: 6193-6198
  • Peer Reviewed
• [Journal Article] Translation from nonautonomous type IAP retrotransposon is a critical determinant of transposition activity : implication for retrotransposon-mediated genome evolution (2008)
  • Author(s): Saito ES, et al
  • Journal Title: Genome Res. 18 Pages: 859-68
  • Peer Reviewed
• [Journal Article] Insertional mutagenesis of the mouse germline with Sleeping Beauty transposition (2008)
  • Author(s): Takeda J, et al
  • Journal Title: Methods Mol Biol. 435 Pages: 109-25
  • Peer Reviewed
• [Journal Article] Germline mutagenesis mediated by Sleeping Beauty transposon system in mice. (2007)
  • Author(s): Takeda VW.Keng, K Horie
  • Journal Title: Genome Biol. 8(Suppl 1)
  • Peer Reviewed
• [Journal Article] Germline mutagenesis mediated by Sleeping Beauty transposon system in mice (2007)
  • Author(s): Takeda J.
  • Journal Title: Genome Biol. 8
  • Peer Reviewed
• [Journal Article] Retrotransposons Influence the Mouse Transcriptome: Implication for the Divergence of Genetic Traits (2007)
  • Author(s): Horie K.
  • Journal Title: Genetics 176 Pages: 815-827
  • Peer Reviewed
• [Presentation] レトロウィルスおよびDNA型トランスポゾンベクターのゲノムワイドな挿入部位の比較解析 (2010)
  • Author(s): 吉田純子、國府力、竹田潤二、堀江恭二
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-12-08
• [Presentation] To12 transposonを用いたマウスES細胞ゲノムへの変異導入 (2009)
  • Author(s): 吉田純子、國府力、川上浩一、竹田潤二、堀江恭二
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-12-11
• [Presentation] Bi-allelically mutated ES cell bank readily applicable for functional analysis (2009)
  • Author(s): Takeda J
  • Organizer: Wellcome Trust Advanced Course : Genetic Manipulation of ES Cells
  • Place of Presentation: Wellcome Trust Sanger Institute (UK)
  • Year and Date: 2009-11-02
• [Presentation] Comprehensive production of a bi-allelically mutated ES cell bank (2008)
  • Author(s): Junji Tekada
  • Organizer: 8TH INTERNATIONAL Transgenic Technology meeting
  • Place of Presentation: Mount Sinai Hospital, Samuel Lunenfeld Research Institute
• [Remarks] ホームページ等
  • URL: http://www.med.Osaka-u.ac.jp/pub/mr-envi/www/index.html
• [Patent(Industrial Property Rights)] 異常行動を示す遺伝子改変非ヒト哺乳動物及びその作製方法 (2008)
  • Inventor(s): 竹田潤二、堀江恭二、八戸宏二郎、山田清文、田熊一敞
  • Industrial Property Rights Holder: 財団法人大阪産業振興機構、国立大学法人大阪大学、国立大学法人金沢大学
  • Filing Date: 2008-07-28