Mutagenesis using transposon system in C57BL/6 mice
| Project/Area Number |
19200034
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
TAKEDA Junji Osaka University, 先端科学イノベーションセンター, 教授 (50163407)
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| Co-Investigator(Kenkyū-buntansha) |
HORIE Kyoji 大阪大学, 医学系研究科, 准教 (30333446)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2009: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2007: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
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| Keywords | トランスポゾンシステム / ミュタゲネーシス / C57BL / 6マウス / 表現型解析 / 病態モデル / トランスポゾン / C57BL/6マウス |
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| Research Abstract |
We have succeeded generation of mutant mice with Sleeping Beauty transposon system. In this project, we established such system in C57BL/6 mice. To stably express SB transposase, we targeted the transposase gene into Rosa26 locus using C57BL/6 ES cells. We are able to distribute the ES cell line.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] SMOC1 is essential for ocular and limb development in humans and mice.2011
Author(s)
Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H.
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Journal Title
Am J Hum Genet. 88
Pages: 30-41
Related Report
Peer Reviewed
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[Journal Article] Diacylglycerol kinase β knockout mice exhibit lithium-sensitive behavioral abnormalities.2010
Author(s)
Kakefuda K, Oyagi A, Ishisaka M, Tsuruma K, Shimazawa M, Yokota K, Shirai Y, Horie K, Saito N, Takeda J, Hara H.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Essential role of neuron-enriched diacylglycerol kinase (DGK), DGKbeta in neurite spine formation, contributing to cognitive function.2010
Author(s)
Shirai Y, Kouzuki T, Kakefuda K, Moriguchi S, Oyagi A, Horie K, Morita SY, Shimazawa M, Fukunaga K, Takeda J, Saito N, Hara H.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro.2010
Author(s)
Yagita K, Horie K, Koinuma S, Nakamura W, Yamanaka I, Urasaki A, Shigeyoshi Y, Kawakami K, Shimada S, Takeda J, Uchiyama Y.
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Journal Title
Proc Natl Acad Sci USA. 107(8)
Pages: 3846-3851
Related Report
Peer Reviewed
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[Journal Article] A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in the mouse genome.2009
Author(s)
Kokubu C, Horie K, Abe K, Ikeda R, Mizuno S, Uno Y, Ogiwara S, Ohtsuka M, Isotani A, Okabe M, Imai K, Takeda J
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Journal Title
Nature Genet. 41
Pages: 946-952
Related Report
Peer Reviewed
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