Measurement and control of cellular functions using spatio-temporal electrical stimulation

• Project/Area Number: 19200036
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: The University of Tokyo
• Principal Investigator: JIMBO Yasuhiko The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (20372401)
• Co-Investigator(Kenkyū-buntansha): MABUCHI Kunihiko 東京大学, 大学院・工学系研究科, 教授 (50192349) ; SAKUMA Ichiro 東京大学, 大学院・情報理工学系研究科, 教授 (50178597)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000); Fiscal Year 2010: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2008: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000); Fiscal Year 2007: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
• Keywords: 生体情報・計測 / 電気刺激 / 細胞 / 脳・神経 / 神経科学 / 細胞・組織 / 分子認識 / ナノバイオ

Research Abstract

Modification of cellular activity induced by spatio-temporal electrical stimulation was studied. Continuous application of2Hz stimulus for12hours elicited approximately the same contraction rhythm in cultured cardiomyocytes. In cultured cortical neurons, the history of spatio-temporal stimulation was reflected in their evoked responses. A novel microcavity-array device was developed for ensemble stimulation of embryoid bodies of stem cells. Intracellular calcium transients were elicited by the stimulus, which would be promising as a tool for regulating cell differentiation.

Research Products

• [Journal Article] Device for co-culture of sympathetic neurons and cardiomyocytes using micro-fabrication (2011)
  • Author(s): Takeuchi A., Nakafutami S., Tani H., Mori M., Takayama Y., Moriguchi H., Kotani K., Miwa K., Lee J., Noshiro M., Jimbo Y.
  • Journal Title: Lab Chip DOI:10
  • Peer Reviewed
• [Journal Article] Ensemble recording of electrical activity in neurons derived from P19 embryonal carcinoma cells (2011)
  • Author(s): Takayama Y., Saito A., Moriguchi H., Kotani K., Jimbo Y.
  • Journal Title: Electron.Commun.Jpn. 94 Pages: 9-19
  • NAID: 10021923591
  • Peer Reviewed
• [Journal Article] Development of Spatially-separatedco-culture system of the sympathetic neuron and the cardiomyocyte (2011)
  • Author(s): Takeuchi A., Moriguchi H., Kotani K., Miwa K., Lee J., Noshiro M., Jimbo Y.
  • Journal Title: IEEJ Trans. 6 Pages: 151-156
  • Peer Reviewed
• [Journal Article] Effects of extremely low frequency magnetic fields on neuronal development of P19 embryonal carcinoma cells (2011)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Journal Title: IEEJ Trans. 6 Pages: 157-162
  • Peer Reviewed
• [Journal Article] Optimization of thin-film configuration for light-addressable stimulation electrode holdings (2011)
  • Author(s): Suzurikawa J., Kanzaki R., Nakao M., Jimbo Y., Takahashi H.
  • Journal Title: Electron.Commun.Jpn. 94 Pages: 61-68
  • Peer Reviewed
• [Journal Article] Device for co-culture of sympathetic neurons and cardiomyocytes using microfabrication (2011)
  • Author(s): Takeuchi A., Nakahutami S., Tani H., Mori M., Takayama Y., Moriguchi H., Kotani K., Miwa K., Lee J., Noshiro M., Jimbo Y.
  • Journal Title: Lab Chip Volume: in press(掲載確定)
  • Peer Reviewed
• [Journal Article] 微小電極アレイを用いた上丘スライス自発活動の培養計測 (2011)
  • Author(s): 廣田, 井上, 森口, 高山, 神保
  • Journal Title: 電気学会論文誌C Volume: 131 in press(掲載確定)
  • NAID: 10030526930
  • Peer Reviewed
• [Journal Article] Development of Spatially-Separated Co-culture System of the Sympathetic Neuron and the Cardiomyocyte (2011)
  • Author(s): Takeuchi A., Moriguchi H., Kotani K., Miwa K., Lee J., Noshiro M., Jimbo Y.
  • Journal Title: IEEJ Trans. Volume: 6 Pages: 151-156
  • Peer Reviewed
• [Journal Article] Effects of Extremely Low Frequency Magnetic Fields on Neuronal Development of P19 Embryonal Carcinoma Cells (2011)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Journal Title: IEEJ Trans. Volume: 6 Pages: 157-162
  • Peer Reviewed
• [Journal Article] 微小神経回路における自発カルシウム濃度変化 (2011)
  • Author(s): 斎藤, 森口, 岩淵, 後藤, 高山, 小谷, 神保
  • Journal Title: 電気学会論文誌C Volume: 131 Pages: 613-618
  • NAID: 10027804470
  • Peer Reviewed
• [Journal Article] 微小電極アレイ上のハイドロゲル膜を介した培養神経細胞の活動計測 (2011)
  • Author(s): 後藤, 森口, 高山, 斎藤, 小谷, 神保
  • Journal Title: 電気学会論文誌C Volume: 131 Pages: 29-34
  • NAID: 10027636567
  • Peer Reviewed
• [Journal Article] 交感神経-心筋共培養系における電気刺激の効果 (2010)
  • Author(s): 武内, 谷, 森, 森口, 小谷, 李, 野城, 神保
  • Journal Title: 電気学会論文誌C Volume: 130 Pages: 1139-1144
  • NAID: 10026378901
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 神保, 高山
  • Journal Title: 「シングルセル解析の最前線」分担執筆(神原,松永,植田編)(CMC出版) Pages: 88-94
• [Journal Article] Spontaneous calcium oscillations in cultured cortical networks during development (2009)
  • Author(s): Takayama Y., Moriguchi H., Kotani K., Jimbo.Y.
  • Journal Title: IEEE Trans.BME 56 Pages: 2649-2956
  • Peer Reviewed
• [Journal Article] Light-addressed stimulation under Ca^<2+> imaging of cultured neurons (2009)
  • Author(s): Suzurikawa J., Nakao M., Jimbo Y., Kanzaki R., Takahashi H.
  • Journal Title: IEEE Trans.BME 56 Pages: 2660-2665
  • Peer Reviewed
• [Journal Article] Ensemble stimulation of embryoid bodies using substrate-embedded electrodes (2009)
  • Author(s): Takayama Y., Saito A., Moriguchi H., Jimbo Y.
  • Journal Title: IEEJ Trans. 4 Pages: 734-735
  • Peer Reviewed
• [Journal Article] Spontaneous Calcium Transients in Cultured Cortical Networks During Development (2009)
  • Author(s): Takayama Y, Moriguchi H, Kotani K, Jimbo Y.
  • Journal Title: IEEE Trans.BME 56 Pages: 2949-2956
  • Peer Reviewed
• [Journal Article] Light-Addressed Stimulation Under Ca^<2+> Imaging of Cultured Neuron (2009)
  • Author(s): Suzurikawa J, Nakao M, Jimbo Y, Takahashi H.
  • Journal Title: IEEE Trans.BME 56 Pages: 2660-2665
  • Peer Reviewed
• [Journal Article] Ensemble Stimulation of Embryoid Bodies using Substrate-Embedded Electrodes (2009)
  • Author(s): Takayama Y, Moriguchi H, Saito A, Jimbo Y.
  • Journal Title: IEEJ Trans. 4 Pages: 734-735
  • Peer Reviewed
• [Journal Article] Light-addressed stimulation under Ca2+ imaging of cultured neurons (2009)
  • Author(s): Suzurikawa J., Nakao M., Jimbo Y., Kanzaki R., Takahashi H.
  • Journal Title: IEEE Trans.BME 56 Pages: 2660-2665
  • Peer Reviewed
• [Journal Article] Ensemble Stimulation of Embryoid Bodies using Substrate-Embedded Electrodes (2009)
  • Author(s): Takayama Y, Saito A., Moriguchi H., Jimbo.Y.
  • Journal Title: IEEJ Trans. 4 Pages: 734-735
  • Peer Reviewed
• [Journal Article] 網膜・上丘組織共培養による視覚情報処理系のin vitro再構築 (2009)
  • Author(s): 廣田, 森口, 高山, 神保
  • Journal Title: 電気学会論文誌C 129 Pages: 1923-1928
  • NAID: 10025318771
  • Peer Reviewed
• [Journal Article] マイクロピペット描画法を用いた培養神経細胞のパターニング (2009)
  • Author(s): 後藤、高山、森口、小谷、神保
  • Journal Title: 電気学会論文誌C 129 Pages: 1231-1236
  • NAID: 10025101125
  • Peer Reviewed
• [Journal Article] 電気刺激/計測可能な交感神経と心筋細胞の半独立共培養系の構築 (2009)
  • Author(s): 武内、森口、李、野城、小谷、神保
  • Journal Title: 電気学会論文誌C 129 Pages: 1225-1230
  • NAID: 10025101111
  • Peer Reviewed
• [Journal Article] 幹細胞由来培養神経回路の構築と電気活動計測 (2009)
  • Author(s): 高山、斎藤、森口、小谷、神保
  • Journal Title: 電気学会論文誌C 129 Pages: 8-16
  • NAID: 10023999096
  • Peer Reviewed
• [Journal Article] 幹細胞由来培養神経回路の構築と電気活動計測 (2009)
  • Author(s): 高山, 斎藤, 森口, 小谷, 神保
  • Journal Title: 電気学会論文誌 C129 Pages: 8-16
  • NAID: 10023999096
  • Peer Reviewed
• [Journal Article] Postural-induced phase shift of respiratory sinus arrhythmia and blood pressure variations-insight from respiratory-phase domain analysis (2008)
  • Author(s): Kotani K., Takamasu K., Jimbo Y, Yamamoto Y.
  • Journal Title: Am.J.Physiol. 294
  • Peer Reviewed
• [Journal Article] Postural-induced phase shift of respiratory sinus arrhythmia an dlood pressure variations: insight from respiratory-phase domain analysis. (2008)
  • Author(s): Kotani K. , Takamasu K. , Jimbo Y. , Yamamoto Y.
  • Journal Title: Am J Physiol Heart Circ Physiol 294
  • Peer Reviewed
• [Journal Article] 非対称な三次元微細構造を用いた神経細胞群間の情報伝達方向制御 (2008)
  • Author(s): 服部, 硯川, 神崎, 神保, 浜口, 高橋, 中尾
  • Journal Title: 電気学会論文誌 C128 Pages: 1036-1042
  • NAID: 10021132951
  • Peer Reviewed
• [Journal Article] 光アドレス型刺激電極の薄膜構成最適化 (2008)
  • Author(s): 硯川, 神崎, 中尾, 神保, 高橋
  • Journal Title: 電気学会論文誌 C128 Pages: 1043-1049
  • NAID: 10021132969
  • Peer Reviewed
• [Journal Article] 分子拡散培養法を用いた培養心筋細胞の成長過程における自律拍動リズムの時間変化 (2008)
  • Author(s): 武内, 小川, 森口, 李, 野城, 小谷, 神保
  • Journal Title: 電気学会論文誌 C128 Pages: 1064-1070
  • NAID: 10021133030
  • Peer Reviewed
• [Journal Article] Postural-induced phase shift of respiratory sinus arrhythmia and blood pressure variatlons-insight from respiratory-phase domain analysis (2008)
  • Author(s): Kotani K., Jimbo Y, 他
  • Journal Title: Am.J.Physiol. 294
  • Peer Reviewed
• [Journal Article] MEA-based recording of neuronal activity in vitro (2007)
  • Author(s): Jimbo Y.
  • Journal Title: Arch.Ital.Biol. 145 Pages: 289-297
  • Peer Reviewed
• [Journal Article] Light-addressable electrode with hydrogenated amorphous silicon and low-conductive passivation layer for stimulation of cultured neurons (2007)
  • Author(s): Suzurikawa J., Takahashi H., Kanzaki R., Nakao M., Takayama Y., Jimbo Y.
  • Journal Title: Appl.Phys.Lett. 90 Pages: 9390-9390
  • Peer Reviewed
• [Journal Article] MEA-based recording of neuronal activity in vitro (2007)
  • Author(s): Jimbo Y.
  • Journal Title: Arch.Ita.Biol. 145 Pages: 289-297
  • Peer Reviewed
• [Journal Article] MEA-based recording of neuronal activity in vitro (2007)
  • Author(s): Jimbo Y
  • Journal Title: Arch, Ita.Biol. 145 Pages: 289-297
  • Peer Reviewed
• [Journal Article] Light-addressable electrode with hydrogenated amorphous silicon and low-conductive passivation layer for stimulation of cultured neurons, (2007)
  • Author(s): Suzurikawa J, Jimbo Y. 他
  • Journal Title: Appl.Phys.Lett. 90 Pages: 9390-9390
  • Peer Reviewed
• [Presentation] Analysis of neuronal activity using statistical properties of Avalanche phenomena (2010)
  • Author(s): Iwabuchi S., Goto M., Saito A., Kotani K., Jimbo Y.
  • Organizer: AP-RASC10
  • Place of Presentation: Toyama Toyama Intetnetional conferce center
  • Year and Date: 2010-09-22
• [Presentation] The time course of electrical activities in co-cultured retina and superior colliculus (2010)
  • Author(s): Inoue K., Hirota S., Kotani K., Jimbo Y
  • Organizer: BMEiCON 2010
  • Place of Presentation: Kyoto
  • Year and Date: 2010-08-27
• [Presentation] Effects of electrical stimulation on cultured cadiomyocytes (2010)
  • Author(s): Mori M., Takeuchi A., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: BMEiCON 2010
  • Place of Presentation: Kyoto
  • Year and Date: 2010-08-27
• [Presentation] The measurement of neuronal-network activity using "micropipette drawing" (2010)
  • Author(s): Goto M., Moriguchi H., Saito A., Takayama Y., Kotani K., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam
  • Year and Date: 2010-07-03
• [Presentation] Interaction of P19 cell-derived neuronal networks and mouse cortical networks co-cultured on micro-electrode array (2010)
  • Author(s): Takayama Y., Moriguchi H., Saito A., Kotani K., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam
  • Year and Date: 2010-07-03
• [Presentation] Effects of electrical stimulation in sympathetic neuron-cardiomyocyte co-cultures (2010)
  • Author(s): Takeuchi A., Tani M., Mori M., Kotani K., Miwa K., Lee J., Noshiro M., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam
  • Year and Date: 2010-07-03
• [Presentation] Evaluation of neuronal differentiation of P19EC cells after alternating current magnetic field exposure (2010)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam
  • Year and Date: 2010-07-03
• [Presentation] The measurement of neuronal-network activity using "Micropipette drawing" (2010)
  • Author(s): Goto M., Moriguchi H., Saito A., Takayama Y., Kotani K., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam,Netherland
  • Year and Date: 2010-07-03
• [Presentation] Interaction of P19 cell-derived neuronal networks and mouse cortical networks co-cultured on micro-electrode array (2010)
  • Author(s): Takayama Y., Moriguchi H., Saito A., Kotani K., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam,Netherland
  • Year and Date: 2010-07-03
• [Presentation] Effects of electrical stimulation in sympathetic neuron-cardiomyocyte co-cultures (2010)
  • Author(s): Takeuchi A., Tani M., Mori M., Kotani K., Miwa K., Lee J.K., Noshiro M., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam,Netherland
  • Year and Date: 2010-07-03
• [Presentation] Evaluation of neuronal differentiation of P19EC cells after alterning current magnetic fields exposure (2010)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 7th FENS Forum
  • Place of Presentation: Amsterdam,Netherland
  • Year and Date: 2010-07-03
• [Presentation] Interactions of P19 cell-derived neuronal networks and mouse cortical networks co-cultured on microelectrode array (2010)
  • Author(s): Takayama Y., Moriguchi H., Saito A., Kotani K., Jimbo Y.
  • Organizer: 7th Int.Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen,Germany
  • Year and Date: 2010-06-29
• [Presentation] Spontaneous calcium transients in cultured cortical networks during development (2010)
  • Author(s): Takayama Y.., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 7th Int.Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen,Germany
  • Year and Date: 2010-06-29
• [Presentation] The Effects of AC Magnetic Fields on Neuronal Differentiation and Network Activities of P19EC Cells (2010)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 7th Int.Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen,Germany
  • Year and Date: 2010-06-29
• [Presentation] Effects of electrical stimulation to sympathetic neuron-cadiomyocyte coculture (2010)
  • Author(s): Takeuchi A., Tani M., Mori M., Moriguchi H., Kotani K., Miwa K., Lee J.K., Noshiro M., Jimbo Y.
  • Organizer: 7th Int.Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen,Germany
  • Year and Date: 2010-06-29
• [Presentation] Electrical and Optical Recording of Interactions between Small Neuronal Networks using Needle-Type Microelectrodes (2010)
  • Author(s): Saito A., Moriguchi H., Goto M., Saito A., Takayama Y., Kotani K., Jimbo Y.
  • Organizer: 7th Int.Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen,Germany
  • Year and Date: 2010-06-29
• [Presentation] Recordings of electrical activity in neuronal network patterned on MEA using Micropipette drawing method (2010)
  • Author(s): Goto M., Moriguchi H., Takayama Y., Saito A., Kotani K., Jimbo Y.
  • Organizer: 7th Int.Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen,Germany
  • Year and Date: 2010-06-29
• [Presentation] 網膜-上丘共培養における電気活動の経時変化 (2010)
  • Author(s): 井上, 廣田, 森口, 高山, 小谷, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-23
• [Presentation] 培養心筋細胞の拍動リズムに対する電気刺激の効果 (2010)
  • Author(s): 谷, 武内, 森, 李, 小谷, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-23
• [Presentation] 小規模培養神経回路群における非シナプス性相互作用の光学的計測と時系列解析 (2010)
  • Author(s): 斎藤, 森口, 岩淵, 後藤, 高山, 小谷, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-23
• [Presentation] マイクロ構造物を用いた培養神経回路のパターン化培養 (2010)
  • Author(s): 伊藤, 仲二見, 武内, 齋藤, 高山, 小谷, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-23
• [Presentation] マイクロピペット描画法による神経細胞ネットワークの活動計測 (2010)
  • Author(s): 後藤, 森口, 斎藤, 高山, 小谷, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-23
• [Presentation] ニューロエンジニアリングの新しい展開へ向けて (2010)
  • Author(s): 神保
  • Organizer: 電気学会全国大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-18
• [Presentation] 培養心筋細胞の拍動リズムに対する電気刺激の効果 (2010)
  • Author(s): 谷, 森, 武内, 李, 小谷, 神保
  • Organizer: 医用生体電磁気学シンポジウム
  • Place of Presentation: 沖縄
  • Year and Date: 2010-01-23
• [Presentation] P19細胞の神経分化能に対する低周波磁場の影響 (2010)
  • Author(s): 斎藤, 高山, 森口, 小谷, 神保
  • Organizer: 医用生体電磁気学シンポジウム
  • Place of Presentation: 沖縄
  • Year and Date: 2010-01-23
• [Presentation] 交感神経-心筋共培養系における電気刺激の効果 (2009)
  • Author(s): 武内, 谷, 小谷, 三輪, 李, 野城, 神保
  • Organizer: 第24回生体生理工学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-09-24
• [Presentation] 微細加工基板を用いた胚様体の多数同時電気刺激 (2009)
  • Author(s): 高山, 森口, 齋藤, 伊藤, 小谷, 神保
  • Organizer: 第24回生体生理工学シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-09-24
• [Presentation] In vitroでの聴覚情報処理系計測技術の基礎研究 (2009)
  • Author(s): 仲二見, 高山, 森口, 小谷, 神保, 舘野
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-09-03
• [Presentation] マウス胚性腫瘍細胞(P19細胞)由来の心筋・神経細胞の共培養に関する基礎的検討 (2009)
  • Author(s): 伊藤, 武内, 齋藤, 高山, 小谷, 神保
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-09-03
• [Presentation] 微小電極アレイ基板を用いた培養交感神経・心筋組織の電気活動評価 (2009)
  • Author(s): 谷, 武内, 三輪, 李, 野城, 小谷, 神保
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-09-03
• [Presentation] 小規模培養神経回路における周期的電気活動の計測 (2009)
  • Author(s): 斎藤, 森口, 高山, 小谷, 神保
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-09-03
• [Presentation] ラット海馬由来スフェロイドの形成と電気活動計測 (2009)
  • Author(s): 森口, 斎藤, 高山, 小谷, 神保
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 徳島
  • Year and Date: 2009-09-03
• [Presentation] Development of semi-separated co-culture system of sympathetic neuron and cardiomyocyte (2009)
  • Author(s): Takeuchi A., Moriguchi H., Kotani K., Miwa K., Lee J., Noshiro M., Jimbo Y.
  • Organizer: 31st Ann.Int.IEEE EMBS Conf.
  • Place of Presentation: Minneapolis
  • Year and Date: 2009-09-02
• [Presentation] Developmental effects of low frequency magnetic fields on P19-derived neuronal cells (2009)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 31st Ann.Int.IEEE EMBS Conf.
  • Place of Presentation: Minneapolis
  • Year and Date: 2009-09-02
• [Presentation] Ensemble stimulation of embryoid bodies using microfabricated ITO substrates (2009)
  • Author(s): Takayama Y., Moriguchi H., Saito A., Kotani K., Jimbo Y.
  • Organizer: 31st Ann.Int.IEEE EMBS Conf.
  • Place of Presentation: Minneapolis
  • Year and Date: 2009-09-02
• [Presentation] Developmental Effects of Low Frequency Magnetic Fields on P19-Derived Neuronal Cells (2009)
  • Author(s): Saito A., Takayama Y., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 31^<st> Ann. Int. IEEE EMBS Conf.
  • Place of Presentation: Minneapolis
  • Year and Date: 2009-09-02
• [Presentation] Ensemble Stimulation of Embryoid Bodies using microfabricated ITO Substrates (2009)
  • Author(s): Takayama Y., Moriguchi H., Saito A., Kotani K., Jimbo Y.
  • Organizer: 31^<st> Ann. Int. IEEE EMBS Conf.
  • Place of Presentation: Minneapolis
  • Year and Date: 2009-09-02
• [Presentation] Development of Semi-Separated Co-culture System of Sympathetic Neuron and Cardiomyocyte (2009)
  • Author(s): 武内, 森口, 小谷, 三輪, 李, 野城, 神保
  • Organizer: 31st Ann.Int.IEEE EMBS Conf.
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 2009-09-02
• [Presentation] Developmental Effects of Low Frequency Magnetic Fields on P19-Derived Neuronal Cells (2009)
  • Author(s): 斎藤, 高山, 森口, 小谷, 神保
  • Organizer: 31st Ann.Int.IEEE EMBS Conf.
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 2009-09-02
• [Presentation] Ensemble Stimulation of Embryoid Bodies using microfabricated ITO Substrates (2009)
  • Author(s): 高山, 森口, 斎藤, 小谷, 神保
  • Organizer: 31st Ann.Int.IEEE EMBS Conf.
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 2009-09-02
• [Presentation] In vitroでの聴覚情報処理系計測技術の基礎研究 (2009)
  • Author(s): 仲二見, 高山, 森口, 小谷, 神保, 舘野
  • Organizer: 電気学会バイオ・マイクロシステム研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-23
• [Presentation] 微小電極アレイを用いた無脊椎動物由来培養神経回路の電気活動計測 (2009)
  • Author(s): 斎藤, 高山, 森口, 神保, 川名
  • Organizer: 電気学会バイオ・マイクロシステム研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-07-23
• [Presentation] 網膜-上丘組織共培養による視覚情報処理系の生体外再構築 (2009)
  • Author(s): 廣田、森口、高山、神保
  • Organizer: 日本生体医工学会第48回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Presentation] 神経再生研究のためのPDMS培養チャンバーの作成 (2009)
  • Author(s): 高山、森口、斎藤、小谷、神保
  • Organizer: 日本生体医工学会第48回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Presentation] マイクロピペット描画法を用いた培養神経細胞のパターニング (2009)
  • Author(s): 後藤、森口、高山、小谷、神保
  • Organizer: 日本生体医工学会第48回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Presentation] 交感神経支配のある培養心室筋組織の電気刺激への応答 (2009)
  • Author(s): 武内、森口、小谷、李、野城、神保
  • Organizer: 日本生体医工学会第48回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Presentation] 磁場印加が神経分化へ及ぼす影響の評価 (2009)
  • Author(s): 斎藤、高山、森口、小谷、神保
  • Organizer: 日本生体医工学会第48回大会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-23
• [Presentation] 細胞分化制御を目的とした胚様体電気刺激基板の作成 (2009)
  • Author(s): 高山, 森口, 齋藤, 小谷, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-10
• [Presentation] マイクロピペット描画法を用いた神経細胞パターニング (2009)
  • Author(s): 後藤, 高山, 森口, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-10
• [Presentation] 交感神経支配のある培養心室筋細胞の拍動リズム (2009)
  • Author(s): 武内, 森口, 小谷, 李, 野城, 神保
  • Organizer: 電気学会医用・生体工学研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-10
• [Presentation] Hierarchical oscillatory patterns observed in the spontaneous and evoked activity in cultured small recurrent networks (2008)
  • Author(s): Moriguchi H., Tamai N., Takayama Y., Kotani K., Jimbo Y.
  • Organizer: 6th FENS Forum
  • Place of Presentation: Geneva
  • Year and Date: 2008-07-12
• [Presentation] Neurons derived from P19 embryonal carcinoma cells establish functional neuronal network (2008)
  • Author(s): Takayama Y., Saito A., Moriguchi H., Kotani K., Jimbo Y.
  • Organizer: 6th FENS Forum
  • Place of Presentation: Geneva
  • Year and Date: 2008-07-12
• [Presentation] Characterization of response patterns evoked by light addressed electrical stimulation in cultured neuronal network (2008)
  • Author(s): Suzurikawa J.Nakao M., Jimbo Y., Kanzaki R., Takahashi H.
  • Organizer: 6th Int.Meet.Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen
  • Year and Date: 2008-07-08
• [Presentation] Characterization of Response Patterns Evoked by Light Addressed Electrical Stimulation in Cultured Neuronal Network (2008)
  • Author(s): Suzurikawa J. Nakao M., Jimbo Y., Kanzaki R., Takahashi H.
  • Organizer: 6th Int. Meet. Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen
  • Year and Date: 2008-07-08
• [Presentation] 静磁界およびELF磁界が胚性癌腫細胞P19の細胞分化に及ぼす影響 (2008)
  • Author(s): 斎藤, 高山, 森口, 神保
  • Organizer: 電気学会医用生体工学研究会
  • Place of Presentation: 東京
• [Presentation] H型チャンバーを用いた心筋細胞と交感神経の接合に関する基礎的研究 (2008)
  • Author(s): 武内, 森口, 小川, 李, 小谷, 神保
  • Organizer: 電気学会医用生体工学研究会
  • Place of Presentation: 東京
• [Presentation] 生きたモデル神経回路の量産と電気的活動の計測 (2008)
  • Author(s): 森ロ, 玉井, 高山, 小谷, 神保
  • Organizer: 日本生体医工学会第47回大会
  • Place of Presentation: 神戸
• [Presentation] 電極アレイ基板システムを用いたP19胚性癖腫瘍細胞由来の神経回路網活動の計測 (2008)
  • Author(s): 高山, 森口, 斎藤, 小谷, 神保
  • Organizer: 日本生体医工学会第47回大会
  • Place of Presentation: 神戸
• [Presentation] 電気刺激印加による培養心筋細胞の自律拍動リズムの変化 (2008)
  • Author(s): 武内, 森口, 李, 野城, 小谷, 神保
  • Organizer: 日本生体医工学会第47回大会
  • Place of Presentation: 神戸
• [Presentation] Neurons derived from P19 embryonal carcinoma cells establish functional neuronal network properties (2008)
  • Author(s): Takayama, Y. , Saito A. , Moriguchi. Kotani, Jimbo Y
  • Organizer: 6th FENS Forum
  • Place of Presentation: Geneva, Switzerland
• [Presentation] Hierarchic oscillatory patterns observed in the spontaneous and evoked activity of cultured small recurrent networks (2008)
  • Author(s): Moriguchi H. , Tamai N. , Takayama, Kotani K. , Jimbo Y.
  • Organizer: 6th FENS Forum
  • Place of Presentation: Geneva, Switzerland
• [Presentation] Neurons derived from P19 embryonal carcinoma cells establish functional neuronal network (2008)
  • Author(s): Takayama Y. , Saito A. , Moriguchi, Kotani, Jimbo Y.
  • Organizer: 6th Int. Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen, Germany
• [Presentation] Extracellular Recording from Mass-produced Small Neuronal Networks using Mobile Metal Microelectrodes (2008)
  • Author(s): Moriguchi H. , Takayama Y. , Kotani K. , Jimbo Y.
  • Organizer: 6th Int. Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen, Germany
• [Presentation] Characterization of Response Patterns Evoked by Light Addressed Electrical Stimulation in Cultured Neuronal Networks (2008)
  • Author(s): Suzurikawa, Nakao, Kanzaki, Jimbo Y. , Takahashi H.
  • Organizer: 6th Int. Meeting on Substrate-Integrated Microelectrodes
  • Place of Presentation: Reutlingen, Germany
• [Presentation] Parallel Multipoint Recording of Aligned and Cultured Neurons on Corresponding Micro Channel Array Toward On-Chip Cell Analysis (2008)
  • Author(s): Tonomura W. , Moriguchi H. , Jimbo Y. , Konishi S.
  • Organizer: 30th Ann. Int. IEEE EMBS Conf.
  • Place of Presentation: Vancouver, Canada
• [Presentation] P19胚性癌腫瘍細胞由来の神経回路網活動の観測 (2008)
  • Author(s): 高山, 斎藤, 森口, 小谷, 神保
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 函館
• [Presentation] H型チャンバーを用いた心筋細胞と交感神経の接合に関する基礎的研究 (2008)
  • Author(s): 武内, 森口, 小川, 李, 小谷, 神保
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 函館
• [Presentation] 微小神経回路アレイのネットワーク活動の電気的・光学的計測 (2008)
  • Author(s): 森口, 高山, 小谷, 神保
  • Organizer: 電気学会電子・情報・システム部門大会
  • Place of Presentation: 函館
• [Presentation] 磁場印加がP19 由来神経細胞の機能分化に及ぼす影響 (2008)
  • Author(s): 斎藤, 高山, 森口, 小谷, 神保
  • Organizer: 第23回生体生理工学シンポジウム
  • Place of Presentation: 名古屋
• [Presentation] Effects of ELFMF exposure on differentiation and spontaneous activity of P19EC-derived neuronal cell (2008)
  • Author(s): A. Saito, Y. Takayama, Moriguchi, Kotani, Y. Jimbo
  • Organizer: 3rd International Symposium on Biomedical Engineering
  • Place of Presentation: Bangkok, Thailand
• [Presentation] Site-selective Stimulation and Recording of the Electrical Activity of Cul tured Neuronal Networks using Mobile Microelectrodes (2008)
  • Author(s): H. Moriguchi, Y. Jimbo, 他
  • Organizer: International Symposium on Biological and Physio logical Engineering
  • Place of Presentation: Harbin,China
• [Presentation] Developmental Activity Changes in P19EC-derived Neurons Cultured on Microelect.rode Array (2008)
  • Author(s): Y. Takayama, X. Jimbo, 他
  • Organizer: International Symposium on Biological and Physio logical Engineering
  • Place of Presentation: Harbin,China
• [Presentation] Site-selective Recording of Electrical Activity from Cultured Neuronal Networks Composed of Small Numbers of Neurons using a Mobile Microelectrode and Spray-patterning Method (2007)
  • Author(s): Moriguchi H., Jimbo Y, 他
  • Organizer: 2nd Int.Workshop Single-cell analysis
  • Place of Presentation: Tokyo
• [Presentation] Light-Addressed Stimulation and Simyltaneous Calcium Imaging for Probing Spatio-Temporal Activety of Cultured Neural Network (2007)
  • Author(s): Suzurikawa, J., Jimbo, Y, 他
  • Organizer: 3rd Int. IEEE EMBS Conf. Neural Engineering
  • Place of Presentation: Hawaii,USA
• [Presentation] Spontaneous Calcium Dynamics in the Development of Cultured Cortical Networks (2007)
  • Author(s): Takayama, Y., Jimbo, . Y, 他
  • Organizer: 3rd Int.IEEE EMBS Conf.Neural Engineering
  • Place of Presentation: Hawati,USA
• [Presentation] Site-Selective Electrical Recording from Small Neuronal Circuits Using Spray Patterning Method and Mobile Microelectrodes (2007)
  • Author(s): Moriguchi, H., Jimbo, Y, 他
  • Organizer: 3rd Int.IEEE EMBS Conf.Neural Engineering
  • Place of Presentation: Hawah,USA
• [Presentation] 培養心筋細胞成長過程における自律拍動リズム変化 (2007)
  • Author(s): 武内 神保, 他
  • Organizer: 電気学会電子情報システム部門大会
  • Place of Presentation: 大阪
• [Presentation] 微小電極アレイを用いたP19細胞分化過程の評価 (2007)
  • Author(s): 齋藤 神保, 他
  • Organizer: 電気学会電子情報システム部門大会
  • Place of Presentation: 大阪
• [Presentation] 培養神経回路成長過程における自発Cawave現象の役割 (2007)
  • Author(s): 高山 神保, 他
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 仙台
• [Presentation] 可動式微小電極による培養細胞の電気的活動の位置選択的・非侵襲的計測法の開発 (2007)
  • Author(s): 森口 神保, 他
  • Organizer: 第46回日本生体医工学会大会
  • Place of Presentation: 仙台
• [Book] シングルセル解析の最前線(分化誘導細胞によって形成した神経回路の電気活動)(神原,松永,植田編) (2010)
  • Author(s): 神保, 高山
  • Publisher: CMC出版
• [Book] 分化誘導細胞によって形成した神経回路の電気活動(神原, 松永, 植田編)「シングルセル解析の最前線」分担執筆 (2010)
  • Author(s): 神保, 高山
  • Publisher: CMC出版
• [Book] 理工系からの脳科学入門(神経回路活動の計測)(合原,神崎編) (2008)
  • Author(s): 神保
  • Publisher: 東京大学出版会
• [Book] 神経回路活動の計測(合原, 神崎編)「理工系からの脳科学入門」分担執筆 (2008)
  • Author(s): 神保
  • Publisher: 東京大学出版会
• [Book] 動物実験代替のためのバイオマテリアル・デバイス(電極アレイシステムを用いる神経機能解析)(酒井,民谷編) (2007)
  • Author(s): 神保, 森口, 高山
  • Publisher: CMC出版
• [Book] 電極アレイシステムを用いる神経機能解析(酒井, 民谷編)「動物実験代替のためのバイオマテリアル・デバイス」分担執筆 (2007)
  • Author(s): 神保, 森口, 高山
  • Publisher: CMC出版
• [Remarks] ホームページ等
  • URL: http://neuron.k.u-tokyo.ac.jp/
• [Remarks]
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• [Remarks]
  • URL: http://neuron.k.u-tokyo.ac.jp
• [Remarks]
  • URL: http://neuron.k.u-tokyo.ac.jp/
• [Remarks]
  • URL: http://neuron.k.u-tokyo.ac.jp/