| Budget Amount *help |
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2009: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2007: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
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| Research Abstract |
Targeted genes and relatives DNA RNA protein and drug involved delivery has received much attention throughout the world. In this study, we show for the first time pH-sensitive delivery of gene and relatives based on biocompatible inorganic nanoparticles of carbonate apatite being highly stable at the typical physiological pH and quickly degradable at the typical pH of endosomal compartment. This nano sized (100-300nm) apatite-gene conjugates demonstrated huge uptake of drug to various cells as determined by Florescence microscopy and flow cytometry. The application of carbonate apatite nanocarriers systems to stem-cells (ES, EC Cells) are successfully done with aid of cell-recogonizable nano-biomaterials such as chimeric antibodies as well as sugar-carrying polymers design.
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