Regulation of Stem Cells Gene Expression using Apatite nanocarriers coated with Cell-reconizable chimeric Protein.
| Project/Area Number |
19200038
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
AKAIKE Toshihiro Tokyo Institute of Technology, フロンティア研究センター, 教授 (30101207)
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| Co-Investigator(Kenkyū-buntansha) |
長岡 正人 東京工業大学, 大学院・生命理工学研究科, 特別研究員 (90397050)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2009: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2007: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
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| Keywords | 薬物伝達システム再生医療 / 炭酸アパタイト / ナノキャリアー / 細胞認識性バイオマテリアル / ES細胞 / m / Si-RNAデリバリー / DNAデリバリー / GFP遺伝子 / フィブロネクチン / 制ガン剤、DDS / 糖鎖高分子 / 遺伝子デリバリー / プロテインデリバリー / E-カドヘリン-FCキメラタンパク質 / ナノキャリア / 細胞認識性 / バイオマテリアル |
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| Research Abstract |
Targeted genes and relatives DNA RNA protein and drug involved delivery has received much attention throughout the world. In this study, we show for the first time pH-sensitive delivery of gene and relatives based on biocompatible inorganic nanoparticles of carbonate apatite being highly stable at the typical physiological pH and quickly degradable at the typical pH of endosomal compartment. This nano sized (100-300nm) apatite-gene conjugates demonstrated huge uptake of drug to various cells as determined by Florescence microscopy and flow cytometry. The application of carbonate apatite nanocarriers systems to stem-cells (ES, EC Cells) are successfully done with aid of cell-recogonizable nano-biomaterials such as chimeric antibodies as well as sugar-carrying polymers design.
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Disrupting actin filaments promotes efficient transfection of a leukemia cell line using cell adhesive protein-embedded carbonate apatite particles2009
Author(s)
K.Kutsuzawa, S.Tada, S.Hossain, K.Fukuda, K.Maruyama, Y.Akiyama, T.Akaike, E.H.Chowdhury,
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Journal Title
Anal. Biochem. 388, 164-166, (2009) 388
Pages: 164-166
Related Report
Peer Reviewed
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[Journal Article] Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation2008
Author(s)
Akaike, T. Hoshiba, T, Kim, S-H. Kobayashi, K. Goto, M. Jiang, H-L. Cho C-S.
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Journal Title
"Tissue Engineering ; Roles, Materials and Applications" S. J. Barners and L. P. Harries Eds, Nova Science Pubishers, Inc
Pages: 99-118
Related Report
Peer Reviewed
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