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Integrated construction of genotypic and phenotypic data of microorganisms relating to the cause of deterioration of the Takamatsuzuka Tumulus mural paintings

Research Project

Project/Area Number 19200057
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Cultural property science
Research InstitutionNational Research Institute for Cultural Properties, Tokyo

Principal Investigator

SANO Chie  National Research Institute for Cultural Properties, Tokyo, 保存修復科学センター・保存科学研究室, 室長 (40215885)

Co-Investigator(Kenkyū-buntansha) KIGAWA Rika  独立行政法人国立文化財機構東京文化財研究所, 保存修復科学センター・生物科学研究室, 室長 (40261119)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2008: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2007: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Keywords微生物 / バイオリソース / 遺伝子 / 古墳壁画 / 漆喰 / 劣化 / 保存科学 / 微生物劣化 / 遺伝子解析 / 装飾古墳 / 漆喰壁画 / カビ / バクテリア / 有機酸分析 / 環境材料 / 文化財科学
Research Abstract

This study aimed to investigate the microbial diversity which was involved in the biodeterioration of murals in the Takamatsuzuka Tumulus. To elucidate the cause of biodeterioration, we carefully identified using an integrated analysis of phenotypic and genotypic characters. The major bacterial isolates were also identified at the generic level using the same methodology. As a result, the major fungal taxa relating to biodeterioration of murals were assignable to Fusarium solani species complex, some Trichoderma spp., Acremonium (sect. Gliomastix) masseei and Penicillium paneum. Some Candida and Pichia and bacterial species in addition to molds species were involved in the formation of biofilms on plaster walls. The selected isolates and their DNA sequences determined in this study were deposited in the public culture collection and public gene bank, respectively.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (33 results)

All 2011 2010 2009 2008

All Journal Article (14 results) (of which Peer Reviewed: 9 results) Presentation (19 results)

  • [Journal Article] Molecular assessment of fungi in "black spots" that deface murals in the Takamatsuzuka and Kitora Tumuli in Japan : Acremonium sect. Gliomastix including Acremonium tumulicola sp. nov. and Acremonium felinum comb. nov.2011

    • Author(s)
      Tomohiko Kiyuna、Kwang-Deuk An, Rika Kigawa, Chie Sano, Sadatoshi Miura, Junta Sugiyama
    • Journal Title

      Mycoscience 52

      Pages: 1-17

    • NAID

      10027837028

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular assessment of fungi in "black spots"that deface murals in the Takamatsuzuka and Kitora Tumuli in Japan : Acremomum sect.Gliomastix including Acremonium tumulicola sp.nov.and Acremonium felinum comb.nov.2011

    • Author(s)
      Tomohiko Kiyuna, Kwang-Deuk An, Rika Kigawa, Chie Sano, Sadatoshi Miura, Junta Sugiyama
    • Journal Title

      Mycoscience

      Volume: 52 Pages: 1-17

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高松塚古墳石室内より分離された主要な微生物のギ酸・酢酸生成能2010

    • Author(s)
      佐野千絵・西島美由紀、喜友名朝彦、木川りか、杉山純多
    • Journal Title

      保存科学 49

      Pages: 209-220

    • NAID

      40017078662

    • URL

      http://www.tobunken.go.jp/~hozon/pdf/49/4921.pdf

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高松塚古墳石室内より分離された主要な微生物のギ酸・酢酸生成能2010

    • Author(s)
      佐野千絵・西島美由紀, 喜友名朝彦, 木川りか, 杉山純多
    • Journal Title

      保存科学 49

      Pages: 209-220

    • NAID

      40017078662

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] The identity of Penicillium sp.1, a major contaminant of the stone chambers in the Takamatsuzuka and Kitora Tumuli in Japan, is Penicillium paneum2009

    • Author(s)
      Kwang-Deuk An, Tomohiko Kiyuna, Rika Kigawa, Chie Sano, Sadatoshi Miura, Junta Sugiyama
    • Journal Title

      Antonie van Leeuwenhoek 96

      Pages: 579-592

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Candida tumulicola sp. nov. and Candida takamatsuzukensis sp. nov., novel yeast species assignable to the Candida membranifaciens clade, isolated from the stone chamber of the Takamatsuzuka tumulus2009

    • Author(s)
      Yuka Nagatsuka, Tomohiko Kiyuna, Rika Kigawa, Chie Sano, Sadatoshi Miura, Junta Sugiyama
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 59

      Pages: 186-194

    • Related Report
      2010 Final Research Report 2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The identity of Penicillium sp. 1, a major contaminant of the stone chambers in the Takamatsuzuka and Kitora Tumuli in Japan, is Penicillium paneum2009

    • Author(s)
      Kwang-Deuk An, Tomohiko Kiyuna, Rika Kigawa, Chie Sano, Sadatoshi Miura, Junta Sugiyama
    • Journal Title

      Antonie van Leeuwenhoek 96

      Pages: 579-592

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] Candida tumulicola sp. nov. and Candida takamatsuzukensis sp. nov., novel yeast species assignable to the Candida membranifaciens Glade, isolated from the stone chamber of the Takamatsuzuka tumulus2009

    • Author(s)
      Yuka Nagatsuka Tomohiko Kiyuna Rika Kigawa Chie Sano Sadatoshi Miura Junta Sugiyama
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology 59

      Pages: 186-194

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] キトラ古墳の微生物等の状況報告(2008)2009

    • Author(s)
      木川りか, 佐野千絵, 間渕創, 喜友名朝彦, 立里臨, 西島美由紀, 杉山純多
    • Journal Title

      保存科学 48

      Pages: 167-174

    • NAID

      40016589694

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mycobiota of the Takamatsuzuka and Kitora Tumuli in Japan, focusing on the molecular phylogenetic diversity of Fusarium and Trichoderma2008

    • Author(s)
      Tomohiko Kiyuna, Kwang-Deuk An, Rika Kigawa, Chie Sano, Sadatoshi Miura, Junta Sugiyama
    • Journal Title

      Mycoscience 49

      Pages: 298-311

    • NAID

      10021906290

    • Related Report
      2010 Final Research Report 2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高松塚古墳発掘・解体作業に伴う生物調査の概要について2008

    • Author(s)
      木川りか・杉山純多・高鳥浩介・間渕創・佐野千絵・三浦定俊
    • Journal Title

      保存科学 47

      Pages: 121-128

    • NAID

      40015981641

    • URL

      http://www.tobunken.go.jp/~hozon/pdf/47/4712.pdf

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mycobiota of the Takamatsuzuka and Kitora Tumuli in Japan, focusing on the molecular phylogenetic diversity of Fusarium and Trichoderma2008

    • Author(s)
      Tomohiko Kiyuna・Kwang-Deuk An・Rika Kigawa Chie Sano・Sadatoshi Miura・Junta Sugiyama
    • Journal Title

      Mycoscience 49

      Pages: 298-311

    • NAID

      10021906290

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] 2008高松塚古墳発掘・解体作業に伴う生物調査の概要について2008

    • Author(s)
      木川りか・杉山純多・高鳥浩介・間渕創・佐野千絵・三浦定俊
    • Journal Title

      保存科学 47

      Pages: 121-128

    • Related Report
      2009 Self-evaluation Report
  • [Journal Article] 2008高松塚古墳発掘・解体作業に伴う生物調査の概要について2008

    • Author(s)
      木川 りか・杉山 純多・高鳥 浩介・間渕 創・佐野 千絵・三浦 定俊
    • Journal Title

      保存科学 47

      Pages: 121-128

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 高松塚古墳壁画やキトラ古墳壁画に危害をもたらすカビとは?2010

    • Author(s)
      杉山純多
    • Organizer
      日本菌学会・日本防菌防黴学会合同シンポジウム「文化財の生物劣化を防ぐための菌類科学の挑戦」
    • Place of Presentation
      東京
    • Year and Date
      2010-11-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Mycobiota in the stone chamber interior and its neighboring environment of the Takamatsuzuka Tumulus in Nara, Japan : Insight into biodeterioration of mural paintings and plaster walls2010

    • Author(s)
      Junta Sugiyama, Tomohiko Kiyuna, Kwang-Deuk An, Yuka Nagatsuka, Rika Kigawa, Chie Sano
    • Organizer
      The Biology of Fungi (IMC9) Edinburgh
    • Place of Presentation
      UK
    • Related Report
      2010 Final Research Report
  • [Presentation] 高松塚古墳石室内より分離された主要な微生物のギ酸・酢酸生成能2010

    • Author(s)
      佐野千絵, 西島美由紀, 喜友名朝彦, 木川りか, 杉山純多
    • Organizer
      日本文化財科学会
    • Place of Presentation
      吹田
    • Related Report
      2010 Final Research Report
  • [Presentation] 漆喰材料の水分特性の比較研究2010

    • Author(s)
      林美木子, 佐野千絵, 石崎武志
    • Organizer
      日本文化財科学会
    • Place of Presentation
      吹田
    • Related Report
      2010 Final Research Report
  • [Presentation] 高松塚古墳石室内およびその周辺環境における菌類相と壁画の生物劣化との関わり2010

    • Author(s)
      喜友名朝彦, 安光得, 木川りか, 佐野千絵, 三浦定俊, 杉山純多
    • Organizer
      日本菌学会第54回大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] 高松塚古墳石室内より分離された主要な微生物のギ酸・酢酸生成能2010

    • Author(s)
      佐野千絵, 西島美由紀, 喜友名朝彦, 木川りか, 杉山純多
    • Organizer
      日本文化財科学会
    • Place of Presentation
      関西大学(吹田市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mycobiota in the stone chamber interior and its neighboring environment of the Takamatsuzuka Tumulus in Nara, Japan : Insight into biodeterioration of mural paintings and plaster walls2010

    • Author(s)
      Junta Sugiyama, Tomohiko Kiyuna, Kwang-Deuk An, Yuka Nagatsuka, Rika Kigawa, Chie Sano
    • Organizer
      The Biology of Fungi
    • Place of Presentation
      Edinburgh, UK
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高松塚古墳石室内およびその周辺環境における菌類相と壁画の生物劣化との関わり2010

    • Author(s)
      喜友名朝彦, 安光得, 木川りか, 佐野千絵, 三浦定俊, 杉山純多
    • Organizer
      日本菌学会第54回大会
    • Place of Presentation
      玉川大学(東京都)
    • Related Report
      2010 Annual Research Report
  • [Presentation] キトラ古墳石室内より分離された酢酸菌2新種および二、三の系統分類学的問題2009

    • Author(s)
      立里臨、半田豊、西島美由紀、木川りか、佐野千絵、杉山純多
    • Organizer
      第19回日本微生物系統分類研究会年次大会
    • Place of Presentation
      木更津
    • Related Report
      2010 Final Research Report 2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] Surface condition of the murals of the Takamatsuzuka tumulus investigated by observation techniques2009

    • Author(s)
      Naoto Yoshida、Yosei Kozuma、Junko Fuirihata、Yoshikazu Tsujimoto、Yasuhiro Hayakawa、Chie Sano、Sadatoshi Miura、Takayasu Koezuka、Shigemasa Udagawa
    • Organizer
      東アジア文化遺産保存学会第1回大会
    • Place of Presentation
      北京(中国)
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 国宝高松塚古墳壁画の材料調査の変遷,2009

    • Author(s)
      佐野千絵、早川泰弘、三浦定俊
    • Organizer
      日本文化財科学会第26回大会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] 国指定史跡「屋形古墳群」珍敷塚古墳保存施設内の温湿度環境2009

    • Author(s)
      佐野千絵、三浦定俊
    • Organizer
      文化財保存修復学会第31回大会
    • Place of Presentation
      倉敷
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 国宝高松塚古墳壁画の材料調査の変遷2009

    • Author(s)
      佐野千絵、早川泰弘、三浦定俊
    • Organizer
      日本文化財科学会第26回大会
    • Place of Presentation
      名古屋
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高松塚古墳・キトラ古墳石室の微生物調査:漆喰壁画の生物劣化にかかわる原因究明の一里塚2008

    • Author(s)
      杉山純多、喜友名朝彦、安光得、小出知己、木川りか、佐野千絵、三浦定俊
    • Organizer
      第31回文化財の保存と修復に関する国際研究集会
    • URL

      http://www.tobunken.go.jp/~hozon/publications/symp2008/int'l-symposium_2008_sugiyama.pdf

    • Place of Presentation
      東京
    • Year and Date
      2008-02-06
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] 微生物繁殖状況モニタリングとしての室内大気分析の有効性2008

    • Author(s)
      佐野千絵・間渕創
    • Organizer
      室内環境学会2008年度大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 高松塚古墳の石室解体中に採取された試料の細菌群集解析2008

    • Author(s)
      西島美由紀、富田順子、中村葵、下村謙悟、立里臨、半田豊、喜友名朝彦、木川りか、佐野千絵、三浦定俊、杉山純多
    • Organizer
      第24回日本微生物生態学会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 高松塚・キトラ両古墳石室内壁画面に発生した"黒いしみ"の正体2008

    • Author(s)
      喜友名朝彦、安光得、木川りか、佐野千絵、三浦定俊、杉山純多
    • Organizer
      日本菌学会第52回大会
    • Place of Presentation
      三重
    • Related Report
      2010 Final Research Report
  • [Presentation] 高松塚・キトラ古墳から分離した主要なPenicillium spp.の分子系統解析法の評価:菌類DNAバーコード化に向けて2008

    • Author(s)
      安光得、喜友名朝彦、木川りか、佐野千絵、三浦定俊、杉山純多
    • Organizer
      日本農芸化学会 2008年度(平成20年度)大会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] 高松塚・キトラ古墳から分離した主要なPenicillium spp.の分子系統解析法の評価:菌類DNAバーコード化に向けて2008

    • Author(s)
      安 光得、喜友名 朝彦、木川 りか、佐野 千絵、三浦 定俊、杉山 純多
    • Organizer
      日本農芸化学会2008年度(平成20年度)大会
    • Place of Presentation
      名古屋
    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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