Development and application of functionalized nanocapsules

• Project/Area Number: 19201024
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: HANDA Hiroshi Tokyo Institute of Technology, 大学院・生命理工学研究科, 教授 (80107432)
• Co-Investigator(Kenkyū-buntansha): OCHIYA Takahiro 国立がんセンター, がん転移研究室, 独立室長 (60192530) ; 阿部 正紀 東京工業大学, 理工学研究科, 教授 (70016624)
• Co-Investigator(Renkei-kenkyūsha): ABE Masanori 東京工業大学, 大学院・理工学研究科, 教授 (70016624)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥39,390,000 (Direct Cost: ¥30,300,000、Indirect Cost: ¥9,090,000); Fiscal Year 2008: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2007: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
• Keywords: ウイルスカプシドタンパク質 / VP1五量体 / 自己組織化 / VP2 / VP3 / 高機能性ナノカプセル / ナノカプセル誘導因子 / MRI造影剤 / 新規DDS / ナノカプセル

Research Abstract

SV40 メジャ-カプシドタンパク質であるVP1 五量体の自己組織化によるナノカプセル形成技術を開発し、それを基盤として、カプセル内への多様な生理活性物質の内包技術およびカプセル表面の修飾・加工による細胞・組織指向性の改変技術を世界に先駆けて開発した。これにより、従来には無い革新的なDDS キャリヤの作製システムが構築できた。

Research Products

• [Journal Article] SV40 vectors carrying minimal sequence of viral origin with exchangeable capsids (2008)
  • Author(s): Nakanishi, A., Chapellie, B., Maekawa, N., Hiramoto, M., Kuge, T., Takahashi, R-H. Handa, H. and Imai, T.
  • Journal Title: Virology 379 Pages: 110-117
  • Peer Reviewed
• [Journal Article] Presentation of functional foreign peptides on the surface of SV40 virus-like particles (2008)
  • Author(s): Takahashi, R-H., Kanesashi, S-N., Inoue, T., Enomoto, T., Kawano, M-A., Tsukamoto, H., Takeshita, F., Imai, T., Ochiya, T., Kataoka, K., Yamaguchi, Y. and Handa, H
  • Journal Title: J. Biotechnol 135 Pages: 385-392
  • Peer Reviewed
• [Journal Article] Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with minor capsid proteins VP2/3 (2008)
  • Author(s): Inoue. T., Kawano, M., Takahashi, R., Tsukamoto, H., Enomoto, T., Imai, T., Kataoka, K. and Handa, H
  • Journal Title: J. Biotechnol 134 Pages: 181-192
  • Peer Reviewed
• [Journal Article] SV40 vectors carrying minimal sequence of viral origin with exchangeable capsids. (2008)
  • Author(s): Nakanishi, A., Chapellie, B., Maekawa, N., Hiramoto, M, Kuge, T., Takahashi, R-H. Handa, H., Imai, T
  • Journal Title: Virology 379 Pages: 110-117
  • Peer Reviewed
• [Journal Article] Presentation of functional foreign peptides on the surface of SV40 virus-like particles. (2008)
  • Author(s): Takahashi, R-H., Kanesashi, S-N., Inoue, T., Enomoto, T., Kawano, M-A., Tsukamoto, H., Takeshita, F., Imai, T., Ochiya, T., Kataoka, K., Yamaguchi, Y., Handa, H.
  • Journal Title: J. Biotechnol 135 Pages: 385-392
  • Peer Reviewed
• [Journal Article] Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with minor capsid proteins VP2/3. (2008)
  • Author(s): Inoue. T., Kawano, M., Takahashi, R., Tsukamoto, H., Enomoto, T., Imai, T., Kataoka, K., Handa, H.
  • Journal Title: J. Biotechnol 134 Pages: 181-192
  • Peer Reviewed
• [Journal Article] Engineering of SV40-based nano-capsules for delivery of heterologousproteins as fusions with minor capsid proteins VP2/3 (2008)
  • Author(s): Inoue, T., Kawano, M., Takahashi, R., Tsukamoto, H., Enomoto, T., Imai, T., Kataoka, K., and Handa, H.
  • Journal Title: J. Biotechnol 134 Pages: 181-192
  • Peer Reviewed
• [Journal Article] Evidence that SV40 VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles (2007)
  • Author(s): Tsukamoto, H., Kawano, M., Inoue, T., Enomoto, T., Takahashi, R., Yokoyama, N., Yamamoto, N., Imai, T., Kataoka, K., Yamaguchi, Y. and Handa, H
  • Journal Title: Genes Cells 12 Pages: 1267-1279
  • Peer Reviewed
• [Journal Article] Evidence that SV40 VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles. (2007)
  • Author(s): Tsukamoto, H., Kawano, M., Inoue, T., Enomoto, T., Takahashi, R., Yokoyama, N., Yamamoto, N., Imai, T., Kataoka, K., Yamaguchi, Y. and Handa, H.
  • Journal Title: Genes Cells 12 Pages: 1267-1279
  • Peer Reviewed
• [Journal Article] Development of novel magnetic anno-carriers for high-performance affinitypurification.
  • Author(s): Nishio, K., Masaike, Y., Ikeda, M., Narimatsu, H., Gokon, N., Tsubouchi, S., Hatakeyama, M., Sakamoto, S., Hanyu, N., Sandhu, A., Kawaguchi, H., Abe, M. and Handa, H.
  • Journal Title: Colloid and Surface B:Biointerfaces (In press)
  • Peer Reviewed
• [Presentation] ウイルスの特性を利用した遺伝子導入用ナノカプセルの構築(Development of Nano-capsule for GeneDelivery by utilizing the in vitro viruscapsid assembly) (2008)
  • Author(s): 榎本輝也、川野雅章、塚本寛子、井上隆昌、高橋陵宇、半田宏
  • Organizer: 第31 回日本分子生物学会年会・第81 回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド ワールド記念ホール
  • Year and Date: 2008-12-12
• [Presentation] ウイルスの特性を利用した遺伝子導入用ナノカプセルの構築Development of Nano-capsule for Gene Delivery by utilizing the in vitro virus capsid assembly (2008)
  • Author(s): 榎本 輝也, 川野 雅章, 塚本 寛子, 井上 隆昌, 高橋 陵宇, 半田 宏
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランドワールド記念ホール
  • Year and Date: 2008-12-12
• [Presentation] 遺伝子導入用ナノカプセルの構築 (2007)
  • Author(s): 榎本輝也、川野雅章、塚本寛子、井上隆昌、高橋陵宇、半田宏
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-12
• [Book] STRUCTURE-BASED STUDY OF VIRAL REPLICATION (2008)
  • Author(s): Hiroshi Handa, Masaaki Kawano, R.Holland Cheng
  • Publisher: World scientific
• [Book] STRUCTURE-BASED STUDY OF VIRAL REPLICATION (2008)
  • Author(s): Hiroshi Handa, Masaaki Kawano, R. Holland Cheng
  • Total Pages: 638
  • Publisher: World scientific
• [Book] STRUCTURE-BASED STUDY OF VIRAL REPLICATION (2008)
  • Author(s): Hiroshi Handa Masaaki Kawano, R.Holland Cheng
  • Total Pages: 637
  • Publisher: World Scientific
• [Remarks]
  • URL: http://www.handa.bio.titech.ac.jp/j/j-home.html
• [Patent(Industrial Property Rights)] 改変されたウィルスカプシドタンパク質及びその使用 (2008)
  • Inventor(s): 半田宏, 中西章, 金刺進之助,高橋陵宇
  • Industrial Property Rights Holder: 東京工業大学
  • Industrial Property Number: 2007-503799
  • Filing Date: 2008-11-19
• [Patent(Industrial Property Rights)] 改変されたウィルスカプシドタンパク質及びその使用 (2008)
  • Inventor(s): 半田宏, 中西章, 金刺進之助, 高橋陵宇
  • Industrial Property Rights Holder: 東京工業大学
  • Industrial Property Number: 2007-503799
  • Filing Date: 2008-11-19
• [Patent(Industrial Property Rights)] 表面被膜無機物粒子の製造方法 (2007)
  • Inventor(s): 半田宏、畠山士、阿部正紀、坂本聡、政池由佳、西尾広介、北善紀、岸寛
  • Industrial Property Rights Holder: 東京工業大学
  • Industrial Property Number: 2007-194233
  • Filing Date: 2007-07-26
• [Patent(Industrial Property Rights)] 被覆磁性微粒子を用いたバイオセンシング方法及び該方法に用いるバイオセンシング方法及びセンシング装置 (2007)
  • Inventor(s): 半田宏、畠山士、阿部正紀、坂本聡、西尾広介、望月勇輔
  • Industrial Property Rights Holder: 東京工業大学
  • Industrial Property Number: 2007-311860
  • Filing Date: 2007-12-03
• [Patent(Industrial Property Rights)] ポリマー被膜無機物微粒子とその製造方法 (2007)
  • Inventor(s): 半田宏、畠山士、阿部正紀、坂本聡、西尾広介、望月勇輔
  • Industrial Property Rights Holder: 東京工業大学
  • Industrial Property Number: 2007-313073
  • Filing Date: 2007-12-04