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An Integrated Strategy for Unveiling the Function of KAONASHI Unknown (Functional) Genes

Research Project

Project/Area Number 19201038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 基礎ゲノム科学
Research InstitutionKeio University

Principal Investigator

SHIMIZU Nobuyoshi  Keio University, 医学部, 名誉教授 (50162706)

Co-Investigator(Kenkyū-buntansha) ASAKAWA Shuichi  慶應義塾大学, 医学部, 講師 (30231872)
SHIMZU Atsushi  慶應義塾大学, 医学部, 助教 (30327655)
SASAKI Takashi  慶應義塾大学, 医学部, 助教 (70306843)
Research Collaborator YO Go  慶應義塾大学, 医学部, 助教 (10464992)
SHIOHAMA Aiko  慶應義塾大学, 医学部, 助教 (40383731)
KOJIMA Sabinukazuko  慶應義塾大学, 医学部, 研究員 (10445439)
Project Period (FY) 2007 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000)
Fiscal Year 2008: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2007: ¥28,860,000 (Direct Cost: ¥22,200,000、Indirect Cost: ¥6,660,000)
Keywordsメダカ / ノックダウン / モルフォリノ / カオナシ / ドメイン / モチーフ / モルフォリノアンチセンス / ノックダウン解析 / 比較ゲノム解析
Research Abstract

カオナシ遺伝子を統合的に解析するために、慶應ヒト遺伝子データベースを構築し、メダカカオナシ遺伝子のノックダウン解析結果や初期胚における発現パターンによる検索を可能とした。さらにヒトカオナシ遺伝子を培養細胞で強制発現させた細胞内局在解析や、高効率なメダカ発現コンストラクトの開発を行った。タンパク質コード遺伝子のみならず小分子カオナシRNAを対象としてシーケンシングと解析を行った。

Report

(3 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • Research Products

    (21 results)

All 2010 2009 2008 2007 Other

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (6 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Retinoic Acid Signaling Positively Regulates Liver Specification by Inducing wnt2bb Gene Expression in Medaka2010

    • Author(s)
      Negishi, T., Nagai, Y., Asaoka, Y., Namae, M., Mitani, H., Sasaki, T., Shimizu, N., Terai, S., Sakaida, I., Kondoh, H., Katada, T., Furutani-Seiki, M., Nishina, H.
    • Journal Title

      Hepatol 51(3)

      Pages: 1037-1045

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two distinct types of theca cells in the medaka gonad : germ cell-dependent maintenance of cyp19al-expressing theca cells2009

    • Author(s)
      Nakamura, S., Kurokawa, H., Asakawa, S., Shimizu, N., Tanaka M.
    • Journal Title

      Dev Dyn 238(10)

      Pages: 2652-2657

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ethylnitrosourea-induced thymus-defective mutants identify roles of KIAA1440, TRRAP, and SKIV2L2 in teleost organ development2009

    • Author(s)
      Iwanami, N., Okada, M., Hoa, V. Q., Seo, Y., Mitani, H., Sasaki, T., Shimizu. N., Kondoh, H., Furutani-Seiki, M., Takahama, Y.
    • Journal Title

      Eur J Immunol 39(9)

      Pages: 2606-2616

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polycystic Kidney Disease in the Medaka (Oryzias latipes) pc Mutant Caused by a Mutation in the Gli-Similar3 (glis3) Gene.2009

    • Author(s)
      Hashimoto, H., Miyamoto, R., Watanabe, N., Shiba, D., Ozato, K., Inoue, C., Kubo, Y, Koga, A., Jindo, T., Narita, T., Naruse, K., Ohishi, K., Nogata, K., Shin-I, T., Asakawa, S., Shimizu. N., Miyamoto, T., Mochijiki, T., Yokoyama, T., Hori, H., Takeda, T., Kohara, Y., Wakamatsu, Y.
    • Journal Title

      Plos ONE 4(7)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cell Cycle Progression, and Teleost Organ Development2008

    • Author(s)
      Iwanami, N., Higuchi, T., Sasano, Y., Fujiwara, T., Hoa, V. Q., Okada, M., Talukder, S. R., Kunimatsu, S., Li, J., Saito, F., Bhattacharya, C., Matin, A., Sasaki, T., Shimizu. N., Mitani, H., Himmelbauer, H., Momoi, A., Kondoh, H., Furutani-Seiki, M., Takahama, Y.
    • Journal Title

      PLoS Genet 4(8)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Myocyte enhancer factor 2 regulates expression of medaka Oryzias latipes fast skeletal myosin heavy chain genes in a temperature-dependent manner2008

    • Author(s)
      Liang, C. S., Ikeda, D., Kinoshita, S., Shimizu, A., Sasaki, T., Asakawa, S., Shimizu. N., Watabe, S.
    • Journal Title

      Gene 407

      Pages: 42-53

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] UTGB/medaka : Genomic Resource Database for Medaka Biology2008

    • Author(s)
      Ahsan, B., Kobayashi, D., Yamada, T., Kasahara, M., Sasaki, S., Saito, T. L., Nagayasu, Y., Doi, K., Nakatani, Y, Qu, W., Jindo, T., Shimada, A., Naruse, K., Toyoda, A., Kuroki, Y., Fujiyama, A., Sasaki, T., Shimizu, A., Asakawa, S., Shimizu. N., Hashimoto, S. I., Yang, J., Lee, Y. Matsushima, K., Sugano, S., Sakaizumi M, Narita T, Ohishi K, Haga S, Ohta F, Nomoto H, Nogata K, Morishita, T., Endo, T., Shin-I., T., Takeda, H., Kohara, Y., Morishita, S.
    • Journal Title

      Nucleic Acids Res. 36

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] UTGB/medaka : Genomic Resource Database for Medaka Biology2008

    • Author(s)
      Ahsan, B.
    • Journal Title

      Nucleic Acis Res. 36

      Pages: 747-752

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] WDR55 is a Nucleolar Modulator of Ribosomal RNA Synthesis, cell Cycle Progression, and Teleost Organ Developraent2008

    • Author(s)
      Iwanami, N.
    • Journal Title

      PLoS Genet. 4

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Radiation hybrid maps of Medaka chromosomes LG 12, 17, and 222007

    • Author(s)
      Su, F., Osada, Y., Ekker, M., Chevrette, M., Shimizu, A., Asakawa, S., Shiohama, A., Sasaki, T., Shimizu. N., Yamanaka, T., Sasado, T., Mitani, H., Geisler, R., Kondoh, H., Furutani-Seiki, M.
    • Journal Title

      DNA Res 14(3)

      Pages: 135-40

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The hotei mutation of medaka in the anti-Mullerian hormone receptor causes the dysregulation of germ cell and sexual development2007

    • Author(s)
      Morinaga, C., Saito, D., Nakamura, S., Sasaki, T., Asakawa, S., Shimizu, N., Mitani, H., Furutani-Seiki, M., Tanaka, M., Kondoh, H.
    • Journal Title

      PNAS. 104(23)

      Pages: 9691-9696

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] The medaka draft genome and insights into vertebrate genome evolution.2007

    • Author(s)
      Kasahara, M.
    • Journal Title

      Nature 447

      Pages: 714-719

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The DNA Sequence of Medaka Chromosome LG22.2007

    • Author(s)
      Sasaki, T.
    • Journal Title

      Genomics 89

      Pages: 124-133

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] カオナシ遺伝子群の解析およびオンライン画像データベースの構築2008

    • Author(s)
      浅川修一, 清水厚志, 佐々木貴史, 清水信義
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      (神戸ポートアイランド) 神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Final Research Report
  • [Presentation] カオナシ遺伝子群の解析およびオンライン画像データベースの構築2008

    • Author(s)
      浅川 修一
    • Organizer
      第31回日本分子生物学会年会・第80回日本生化学大会合同大会(BMB2008)
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] カオナシ遺伝子群のノックダウン解析およびオンラインデータベースの構築2007

    • Author(s)
      清水厚志, 浅川修一, 佐々木貴史, 清水信義
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      (パシフィコ横浜) 横浜
    • Year and Date
      2007-12-11
    • Related Report
      2008 Final Research Report
  • [Presentation] カオナシ遺伝子群のノックダウン解析およびオンラインデータベースの構築2007

    • Author(s)
      清水 厚志
    • Organizer
      第30回日本分子生物学会年会・第80回日本生化学会大会合同大会(BMB2007)
    • Place of Presentation
      横浜
    • Year and Date
      2007-12-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] The American Society of Human Genetics 57th Annual Meeting2007

    • Author(s)
      Shimizu, A, Asakawa, S., Sasaki, T., Shimizu. N., An On-line Database System for Knock-down Analysis of KAO-NASHI Genes
    • Organizer
      San Diego
    • Year and Date
      2007-10-26
    • Related Report
      2008 Final Research Report
  • [Presentation] An On-line Database System for Knock-down Analysis of KAO-NASHI Genes2007

    • Author(s)
      清水 厚志
    • Organizer
      第56回アメリカ人類遺伝学会
    • Place of Presentation
      米国サンディエゴ
    • Year and Date
      2007-10-26
    • Related Report
      2007 Annual Research Report
  • [Book] Encyclopedia of Life Sciences;Comparing the Human and Medaka Genomes2008

    • Author(s)
      Sasaki, T.
    • Publisher
      NPG(Nature Pubhshing Group)(in press)
    • Related Report
      2007 Annual Research Report
  • [Remarks] N-K高校講座生物第23回 第3部 遺伝 20071015

    • URL

      http://www.nhk.or.jp/kokokoza/tv/seibutsu/archive/resume023.htm

    • Related Report
      2008 Final Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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