Modeling of Biological Wastewater Treatment Processes Based on Microbial Population Analyses

• Project/Area Number: 19206057
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: The University of Tokyo
• Principal Investigator: MINO Takashi The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (60166098)
• Co-Investigator(Kenkyū-buntansha): SATO Hiroyasu 東京大学, 大学院・新領域創成科学研究科, 准教授 (90251347) ; 小貫 元治 東京大学, サステナビリティ学連携研究機構, 特任講師 (20376594)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000); Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2007: ¥33,930,000 (Direct Cost: ¥26,100,000、Indirect Cost: ¥7,830,000)
• Keywords: 生物学的廃水処理 / 活性汚泥法 / リン除去 / 窒素除去 / PHA(ポリヒドロキシアルカン酸) / 上下水道 / 生物学的りん除去 / 亜硝酸型硝化脱窒 / モデル化 / 分子生物学 / 排水処理

Research Abstract

Methods to analyze microbial communities in activated sludg were developed especially to clarify the behaviors of polyphosphate accumulating bacteria and PHA accumulating bacteria. It was found that PHA accumulating organisms mostly belong to α- and β-Proteobacteria. A new method to reduce energy consumption in wastewater treatment was proposed, which utilize PHA accumulating microorganisms. Further, a novel methodology to investigate microbial interactions mediated by chemicals was invented.

Research Products

• [Journal Article] Population dynamics in a sequencing batch reactor fed with glucose and operated for enhanced biological phosphorus removal. (2010)
  • Author(s): Zengin, G.E., Artan, N., Orhon, D., Chua, A.S.M., Satoh H., Mino, T.
  • Journal Title: Biores.Technol. 11 Pages: 4000-4005
  • Peer Reviewed
• [Journal Article] Separation of PHA-Accumulating Cells in Activated Sludge Based on Differences in Buoyant Density (2010)
  • Author(s): Oshiki, M., Onuki, M., Satoh, H., Mino, T
  • Journal Title: J.Gen.Appl.Microbiol.
  • NAID: 130004449283
  • Peer Reviewed
• [Journal Article] Separation of PHA-Accumulating Cells in Activated Sludge Based on Differences in Buoyant Density (2010)
  • Author(s): 押木守, 小貫元治, 佐藤弘泰, 味埜俊
  • Journal Title: J.Gen.Appl.Microbiol. (In press)(印刷中)(掲載確定)
  • NAID: 130004449283
  • Peer Reviewed
• [Journal Article] 活性汚泥プロセスにおける有機性一時貯蔵物質の蓄積 (2009)
  • Author(s): 押木守, 小貫元治, 佐藤弘泰, 味埜俊
  • Journal Title: 下水道協会誌 46(566) Pages: 126-137
  • NAID: 10025871509
  • Peer Reviewed
• [Journal Article] 活性汚泥中の成分が細菌群集構造に及ぼす影響のマイクロプレート培養法を用いた検討 (2009)
  • Author(s): 佐藤弘泰、小川旦、味埜俊
  • Journal Title: 環境工学研究論文集 46 Pages: 503-510
  • Peer Reviewed
• [Journal Article] 微生物の持つ有機物貯蔵能力を利用した省エネルギー型廃水処理法の提案 (2009)
  • Author(s): 押木守, 佐藤弘泰, 味埜俊
  • Journal Title: 用水と廃水 51(9) Pages: 41-49
  • Peer Reviewed
• [Journal Article] リン資源の効率的回収に向けて～頼れる生物学的リン除去活性汚泥法を目指して～ (2009)
  • Author(s): 佐藤弘泰
  • Journal Title: 再生と利用 No.124 Pages: 11-18
• [Journal Article] Microorganisms involved in anaerobic phenol degradation in the treatment of synthetic coke-oven wastewater detected by RNA stable-isotope probing. (2009)
  • Author(s): Sueoka, K., Satoh H., Onuki M., Mino, T
  • Journal Title: FEMS MICROBIOLOGY LETTERS. 291 Pages: 169-174
  • Peer Reviewed
• [Journal Article] 活性汚泥中の成分が細菌群集構造に及ぼす影響のマイクロプレート培養法を用いた検討 (2009)
  • Author(s): 佐藤弘泰, 小川旦, 味埜俊
  • Journal Title: 環境工学研究論文集 46 Pages: 503-510
  • Peer Reviewed
• [Journal Article] Microorganisms involved in anaerobic phenol degradation in the treatment of synthetic coke-oven wastewater detected by RNA stable-isotope probing (2009)
  • Author(s): Sueoka, K., Satoh, H., Onuki, M., Mino, T
  • Journal Title: FEMS MICROBIOLOGY LETTERS 291 Pages: 169-174
  • Peer Reviewed
• [Journal Article] 微生物の持つ有機物貯蔵貯蔵能力を利用した省エネルギー型廃水処理方法の提案 (2009)
  • Author(s): 押木守、佐藤弘泰、味埜俊
  • Journal Title: 用水と廃水 51 Pages: 41-49
  • Peer Reviewed
• [Journal Article] 活性汚泥プロセスにおける有機性-時貯蔵物質の蓄積 (2009)
  • Author(s): 押木守、小貫元治、佐藤弘泰、味埜俊
  • Journal Title: 下水道協会誌 46(566) Pages: 126-137
  • Peer Reviewed
• [Journal Article] リン資源の効率的回収に向けて～頼れる生物学的リン除去活性汚泥法を目指して～ (2009)
  • Author(s): 佐藤弘泰
  • Journal Title: 再生と利用 124 Pages: 11-18
• [Journal Article] Occurrence of Polyhydroxyalkanoate as Temporal Carbon Storage Material in Activated Sludge during The Removal of Organic Pollutants. (2008)
  • Author(s): Mamoru Oshiki,, You Yang, Motoharu Onuki, Hiroyasu Satoh, Yong-Zhen Peng, Takashi Mino
  • Journal Title: Journal of Water Environment Technology 6 Pages: 77-83
  • NAID: 130000098211
  • Peer Reviewed
• [Journal Article] 活性汚泥中微生物群集構造解析のための超音波破砕と希釈による新規DNA抽出手法の開発 (2008)
  • Author(s): 佐藤弘泰、小貫元治、味埜俊
  • Journal Title: 環境工学研究論文集 45 Pages: 225-232
  • NAID: 130003949597
  • Peer Reviewed
• [Journal Article] PHA-accumulating microorganisms in full-scale wastewater treatment plants. (2008)
  • Author(s): Oshiki M., Onuki M., Satoh H., Mino, T
  • Journal Title: WATER SCIENCE AND TECHNOLOGY. 58 Pages: 13-20
  • Peer Reviewed
• [Journal Article] PHA-accumulating microorganisms in full-scall wastewater treatment plants (2008)
  • Author(s): Oshiki M, Onuki M, Satoh H, Mino T
  • Journal Title: WATER SCIENCE AND TECHNOLOGY 58(1) Pages: 13-20
  • Peer Reviewed
• [Journal Article] occurrence of Polyhydroxyalkanoate as Temporal Carbon Storage Material in Activated Sludge during The Removal of Organic Pollutants. (2008)
  • Author(s): Oshiki, M. , Yang, Y. , Onuki, M. l, Satoh, H. , Peng, Y. -Z. and Mino, T.
  • Journal Title: J. Water Environ. Technol. 6 Pages: 77-83
  • NAID: 130000098211
  • Peer Reviewed
• [Journal Article] 活性汚泥中微生物群集構造解析のための超音波破砕と希釈による新規DNA抽出手法の開発 (2008)
  • Author(s): 佐藤弘泰, 小貫元治, 味埜 俊
  • Journal Title: 環境工学研究論文集 45 Pages: 225-232
  • NAID: 130003949597
  • Peer Reviewed
• [Journal Article] Abundance of Candidatus 'Accumulibacter phosphatis' in enhanced biological phosphorus removal activated sludge acclimatized with different carbon sources. (2007)
  • Author(s): Fukushima, T., Uda, N., Okamoto, M., Onuki M., Satoh H., Mino, T
  • Journal Title: MICROBES AND ENVIRONMENTS. 22 Pages: 346-354
  • NAID: 110006473361
  • Peer Reviewed
• [Journal Article] Abundance of Candidatus 'Accumulibacter phosphatis' in enhanced biological phosphorus removal activated sludge acclimatized with different carbon sources (2007)
  • Author(s): Fukushima, T., Uda, N., Okamoto, M., Onuki, M., Satoh, H., Mino, T
  • Journal Title: MICROBES AND ENVIRONMENTS 22 Pages: 346-354
  • NAID: 110006473361
  • Peer Reviewed
• [Journal Article] Abundance of Candidatus'Accumulibacter phosphatis' in Enhanced Biological Phosphorus Removal Activated Sludge Acclimatized with Different Carbon Sources (2007)
  • Author(s): Toshikazu Fukushima, Naoki Uda, Mayuko Okamoto, Motoharu Onuki, Hiroyasu Satoh, Takashi Mino
  • Journal Title: Microbes and Environments 22 Pages: 346-354
  • Peer Reviewed
• [Journal Article] Separation of PHA-Accumulating Cells in Activated Sludge Based on Differences in Buoyant Density
  • Author(s): Oshiki M., Onuki M., Satoh H., Mino, T
  • Journal Title: J.Gen.Appl.Microbiol. In press.(掲載確定) Pages: 2010-2010
  • NAID: 130004449283
  • Peer Reviewed
• [Presentation] 余剰汚泥消化プロセスにおけるポリヒドロキシアルカン酸(PHA)の分解挙動 (2010)
  • Author(s): 押木守, 佐藤弘泰, 味埜俊
  • Organizer: 第44回日本水環境学会講演集.P72
  • Place of Presentation: 福岡市
• [Presentation] 余剰汚泥消化プロセスにおけるポリヒドロキシアルカン酸 (PHA) の分解挙動 (2010)
  • Author(s): 押木守, 佐藤弘泰, 味埜俊
  • Organizer: 第44回日本水環境学会講演集
  • Place of Presentation: 福岡市
• [Presentation] 余剰汚泥消化プロセスにおけるポリヒドロキシアルカン酸(PHA)の分解挙動 (2010)
  • Author(s): 押木守、佐藤弘泰、味埜俊
  • Organizer: 第44回日本水環境学会
  • Place of Presentation: 福岡市
• [Presentation] 一時貯蔵反応を利用した省曝気・有機物回収型廃水処理プロセスの提案 (2009)
  • Author(s): 押木守, 小貫元治, 佐藤弘泰, 味埜俊
  • Organizer: 第43回日本水環境学会年会
  • Place of Presentation: 山口大学(山口市)
  • Year and Date: 2009-03-16
• [Presentation] 微生物のもつ有機物貯蔵能力の有機物回収への利用 (2009)
  • Author(s): 佐藤弘泰, 押木守,小貫元治, 味埜俊
  • Organizer: 第12回水環境学会シンポジウム講演集,164-165
  • Place of Presentation: 東京
• [Presentation] 活性汚泥中細菌による生分解性プラスチックPHAの生産～菌体外に見いだされたPHA顆粒～ (2009)
  • Author(s): 押木守, 佐藤弘泰, 味埜俊, 小貫元治
  • Organizer: 第12回水環境学会シンポジウム講演集,pp166-167
  • Place of Presentation: 東京
• [Presentation] 微生物のもつ有機物貯蔵能力の有機物回収への利用 (2009)
  • Author(s): 佐藤弘泰, 押木守, 小貫元治,味埜俊
  • Organizer: 第12回水環境学会シンポジウム講演集
  • Place of Presentation: 東京
• [Presentation] 活性汚泥中細菌による生分解性プラスチックPHAの生産～菌体外に見いだされたPHA顆粒～ (2009)
  • Author(s): 押木守, 佐藤弘泰, 味埜俊, 小貫元治
  • Organizer: 第12回水環境学会シンポジウム講演集
  • Place of Presentation: 東京
• [Presentation] Development of a novel DNA Extraction Method based on Sonication followed by Dilution for the Microbial Community Analysis of Activated Sludge Samples (2009)
  • Author(s): 佐藤弘泰
  • Organizer: International Workshop on Water and Wastewater Treatment
  • Place of Presentation: Tainan, Taiwan
• [Presentation] 超音波破砕・希釈によるPCR反応用鋳型試料調整法の各種試料への適用 (2009)
  • Author(s): 佐藤弘泰、押木守、近藤宗俊、Gunawardana Wasana, 高梨正訓、鯉渕幸生、味埜俊
  • Organizer: 微生物生態学会第25回大会
  • Place of Presentation: 東広島市
• [Presentation] 超音波破砕・希釈法によるDNA抽出のバイアス評価 (2009)
  • Author(s): 高梨正訓、押木守、小貫元治、佐藤弘泰、味埜俊
  • Organizer: 第46回環境工学研究フォーラム
  • Place of Presentation: 高崎市
• [Presentation] Wastewatercharacteristics and non-acclimatized sludge activity as indicators for evaluating polyhydroxyalkanoates (PHA) production potential of food industrial activated sludge (2009)
  • Author(s): Chiemchaisri, C., Chiemchaisri, W., Buathong, S., 佐藤弘泰
  • Organizer: The 3rd IWA-Aspire Conference
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Acetate Uptake by PHA-Accumulating and non-PHA-Accumulating Organisms in Activated Sludge from Aerobic Sequencing Batch Reactor fed with Acetate (2009)
  • Author(s): 押木守, 佐藤弘泰, 味埜俊
  • Organizer: The 3rd IWA-Aspire Conference
  • Place of Presentation: Taipei, Taiwan
• [Presentation] 微生物のもつ有機物貯蔵能力の有機物回収への利用 (2009)
  • Author(s): 佐藤弘泰、押木守、小貫元治、味埜俊
  • Organizer: 第12回日本水環境学会シンポジウム
  • Place of Presentation: 東京
• [Presentation] 活性汚泥中細菌による生分解プラスチックPHAの生産～菌体外に見出されたPHA顆粒～ (2009)
  • Author(s): 押木守、佐藤弘泰、味埜俊、小貫元治
  • Organizer: 第12回日本水環境学会シンポジウム
  • Place of Presentation: 東京
• [Presentation] 活性汚泥内に存在する細胞外PHA顆粒の観察 (2009)
  • Author(s): 押木守、佐藤弘泰、味埜俊
  • Organizer: 第17回衛生工学シンポジウム
  • Place of Presentation: 札幌、北海道大学学術交流会館
• [Presentation] 廃水処理におけるβおよびγ Proteobacteriaに属するアンモニア酸化細菌の定量 (2008)
  • Author(s): 庄司 仁、小貫 元治、佐藤 弘泰、味埜 俊
  • Organizer: 第24回日本微生物生態学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-11-26
• [Presentation] Characterization of different granule types occurring in an enhanced biological phosphorus removal activated sludge process (2008)
  • Author(s): 福島 寿和、Jeremy Barr、佐藤 弘泰、味埜 俊、Phillip Bond
  • Organizer: 第24回日本微生物生態学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2008-11-26
• [Presentation] PHA-Accumulating Microorganisms in Full-Scale Wastewater Treatment Plants (2008)
  • Author(s): Oshiki, M. , Onuki, M. , Satoh, H. and Mino, T.
  • Organizer: International Water Association (IWA) World Water Congress and Exhibition 2008
  • Place of Presentation: ウィーン(オーストラリア)
  • Year and Date: 2008-09-09
• [Presentation] MICROBIAL PUPULATION OF PHA (POLYHYDROXYALKANOAATE)-ACCUMULATING OTGANISMS IN ACTIVATED SLUDGE AND THEIR DISTRIBUTION IN EIGHT FULL-SCALE WASTEWATER TREATMENT PLANTS (2008)
  • Author(s): Oshiki, M. , Onuki, M. , Satoh, H. and Mino, T.
  • Organizer: International Society for Microbial Ecology(ISME)-12
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2008-08-22
• [Presentation] Development of Real Time Quantitative Reverse Transcription PCR for Candidatus 'Accumulibacter phosphatis' and Its Application to Activated Sludge (2008)
  • Author(s): Toshikazu FUKUSHIMA, Motoharu ONUKI, Hiroyasu SATOH and Takashi MINO
  • Organizer: International Society for Microbial Ecology(ISME)-12
  • Place of Presentation: ケアンズ(オーストラリア)
  • Year and Date: 2008-08-20
• [Presentation] 有機物除去過程における有機性一時貯蔵物質の生成 (2008)
  • Author(s): 押木守, 小貫元治, 佐藤弘泰, 味埜俊
  • Organizer: 有機物除去過程における有機性一時貯蔵物質の生成
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2008-07-22
• [Presentation] Simultaneous thiocyanate and nitrogen removal by anoxic-aerobic activated sludge process under high salinity conditions. (2008)
  • Author(s): Tadashi Shoji, Motoharu Onuki, Hiroyasu Satoh, Takashi Mino
  • Organizer: IWA Chemical Industries 2008
  • Place of Presentation: Beijing, China.
• [Presentation] Development of Real Time Quantitative Reverse Transcription PCR for Candidatus 'Accumulibacter phosphatis' and Its Application to Activated Sludge (2008)
  • Author(s): Toshikazu FUKUSHIMA, Motoharu ONUKI, Hiroyasu SATOH, Takashi MINO
  • Organizer: 12th International Symposium on Microbial Ecology -ISME-12
  • Place of Presentation: Cairns, Australia.
• [Presentation] Development of Real Time Quantitative Reverse Transcription PCR for Candidatus 'Accumulibacter phosphatis' and Its Application to Activated Sludge (2008)
  • Author(s): Toshikazu FUKUSHIMA, Motoharu ONUKI, Hiroyasu SATOH, Takashi MINO
  • Organizer: 12th International Symposium on Microbial Ecology-ISME-12
  • Place of Presentation: Cairns, Australia
• [Presentation] Simultaneous thiocyanate and nitrogen removal by anoxic-aerobic activated sludge process under high salinity conditions (2008)
  • Author(s): Tadashi Shoji, Motoharu Onuki, Hiroyasu Satoh, Takashi Mino
  • Organizer: IWA Chemical Industries 2008
  • Place of Presentation: Beijing, China
• [Presentation] 脱窒性リン蓄積細菌の凝集・分散能に関する基礎的検討 (2008)
  • Author(s): 小堀貴弘, 森亮, 齋藤利晃, 湯本高太, 吉田征史, 福島寿一, 庄司仁, 佐藤弘泰
  • Organizer: 第42回日本水環境学会年会講演集
  • Place of Presentation: 名古屋大学
• [Presentation] 活性汚泥細菌群集解析に用いられるユニバーサルプライマ-の比較 (2008)
  • Author(s): 田崎彩, 小貫元治, 佐藤弘泰, 味埜俊
  • Organizer: 第42回日本水環境学会年会講演集
  • Place of Presentation: 名古屋大学
• [Presentation] 硝化脱窒細菌の窒素負荷履歴を考慮した活性汚泥モデルの開発 (2007)
  • Author(s): 山下哲生, 竹田尚弘, 辻幸志, 藤田昌史, 味埜俊, 赤司昭
  • Organizer: 第44回環境工学研究フォーラム講演集
  • Place of Presentation: 山口大学
• [Presentation] Substrate Competition betwwen PAOs and GAOs in EBPR A Sludge Process (2007)
  • Author(s): Fujiwara, K., Onuki, M., Satoh, H., Mino, T.
  • Organizer: 7th China-Japan Symposium on Water Environment
  • Place of Presentation: 北京(中国)
• [Presentation] Comparison of Different Universal Primers Used for the Bacterial Community Analyses in Activated Sludge (2007)
  • Author(s): Tazaki, A., Onuki, M., Oshiki, M., Satoh, H., Peng, Y-Z., Mino, T.
  • Organizer: 7th China-Japan Symposium on Water Environment
  • Place of Presentation: 北京(中国)
• [Presentation] Analysis of Growth Characteristics of Candidatus 'Accumulibacter phosphatis' by Using Quantitative PCR Method and Its Application to Isolation (2007)
  • Author(s): Fukushima, T., Yano, M., Onuki, M., Satoh, H., Mino, T.
  • Organizer: 7th China-Japan Symposium on Water Environment
  • Place of Presentation: 北京(中国)
• [Presentation] 標準活性汚泥法における有機性-時貯蔵物質の挙動 (2007)
  • Author(s): 近藤光佳, 佐藤弘泰, 小貫元治, 味埜俊泰
  • Organizer: 第44回環境工学研究フォーラム講演集
  • Place of Presentation: 山口大学