Structural and Functional analysis of RND type transporter

• Project/Area Number: 19207009
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Tokyo Institute of Technology (2008-2009); Osaka University (2007)
• Principal Investigator: MURAKAMI Satoshi Tokyo Institute of Technology, 大学院・生命理工学研究科, 教授 (30300966)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000); Fiscal Year 2009: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2008: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2007: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 多剤排出トランスポーター / 結晶化 / X線結晶構造解析 / 多剤耐性 / 膜輸送 / 構造解析 / 薬剤耐性 / 構造生物学 / 線結晶構造解析 / 膜輸送体 / トランスポーター

Research Abstract

Structure of AcrB was improved beyond 2.5Å resolution.
Along the substrate translocation pathway of AcrB, an important region where determine the substrate specificity was found.
Drug transport across the membrane was observed for Gram-positive RND transporter.

Research Products

• [Journal Article] Enhancement of femtosecond laser-induced nucleation of protein in a gel solution(043702) (2010)
  • Author(s): Ryota Murai, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori.
  • Journal Title: Appl. Phys. Lett. 96 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Enhancement of femtodecond laser-induced nucleation of protein in a gel solution (043702) (2010)
  • Author(s): Ryota Murai, Hiroshi Y.Yoshikawa, Yoshinori Takahashi, Mihoko Maruyama, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori.
  • Journal Title: Appl.Phys.Lett. 96 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Femtosecond laser processing of protein crystals grown in agarose gel (2009)
  • Author(s): Hitoshi Hasenaka, Shigeru Sugiyama, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Hiroyoshi Matsumura.
  • Journal Title: J. Cryst. Growth 312 Pages: 73-78
  • Peer Reviewed
• [Journal Article] A Manipulating Tool for Protein Microcrystals in Solution Using Adhesive Materials(118001) (2009)
  • Author(s): Tomoya Kitatani, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano.
  • Journal Title: Jpn. J. Appl. Phys. 48 Pages: 1-2
  • Peer Reviewed
• [Journal Article] Promotion of Crystal Nucleation of Protein by Semi-Solid Agarose Gel(125501) (2009)
  • Author(s): Kana Tanabe, Mika Hirose, Ryota Murai, Shigeru Sugiyama, Noriko Shimizu, Mihoko Maruyama, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Eiichi Mizohata, Tsuyoshi Inoue, Hiroyoshi Matsumura.
  • Journal Title: Appl. Phys. Express 2 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Growth of Large Protein Crystals by Top-Seeded Solution Growth Together with the Floating and Solution-Stirring Technique (2009)
  • Author(s): Noriko Shimizu, Shigeru Sugiyama, Mihoko Maruyama, Hiroshi Y. Yoshikawa, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue Hiroyoshi Matsumura, Yusuke Mori.
  • Journal Title: Cryst. Growth Des. 9 Pages: 5227-5232
  • Peer Reviewed
• [Journal Article] Femtosecond Laser Processing of Agarose Gel Surrounding Protein Crystals for Development of an Automated Crystal Capturing System(105502) (2009)
  • Author(s): Shigeru Sugiyama, Hitoshi Hasenaka, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Hiroyoshi Matsumura.
  • Journal Title: Jpn. J. Appl. Phys. 48 Pages: 1-5
  • Peer Reviewed
• [Journal Article] 多剤耐性 (2009)
  • Author(s): 村上聡
  • Journal Title: Multi-drug resistance Medical Science Digest 35 Pages: 346-347
  • Peer Reviewed
• [Journal Article] Protein Crystallization in Agarose Gel 4 with High Strength: Developing an Automated System for Protein Crystallographic Processes(075502) (2009)
  • Author(s): Shigeru Sugiyama, Kana Tanabe, Mika Hirose, Tomoya Kitatani, Hitoshi Hasenaka, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Tsuyoshi Inoue, Hiroyoshi Matsumura.
  • Journal Title: Jpn. J. Appl. Phys. 48 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Dynamic nature of disulfide bond formation catalysts revealed by crystal structures of DsbB (2009)
  • Author(s): Keiji Inaba, Satoshi Murakami, Atsushi Nakagawa, Hiroka Iida, Mai Kinjo, Koreaki Ito, Mamoru Suzuki.
  • Journal Title: EMBO J. 28 Pages: 779-791
  • Peer Reviewed
• [Journal Article] Femtosecond laser-induced nucleation of protein in agarose gel (2009)
  • Author(s): Hiroshi Y. Yoshikawa, Ryota Murai, Shigeru Sugiyama, Gen Sazaki, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Kazufumi Takano, Yusuke Mori.
  • Journal Title: J. Cryst. Growth 311 Pages: 956-959
  • Peer Reviewed
• [Journal Article] Femtosecond laser-induced nucleation of protein in agarose gel (2009)
  • Author(s): Hiroshi Y.Yoshikawa, Ryota Murai, Shigeru Sugiyama, Gen Sazaki, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Kazufumi Takano, Yusuke Mori.
  • Journal Title: J.Cryst.Growth 311 Pages: 956-959
  • Peer Reviewed
• [Journal Article] Dynamic nature of disulfide bond formation catalysts revealed by crystal structures of DsbB. (2009)
  • Author(s): Keiji Inaba, Satoshi Murakami, Atsushi Nakagwa, Hiroka Iida, Mai Kinjo, Koreaki Ito, Mamoru Suzuki.
  • Journal Title: EMBO J. 28 Pages: 779-791
  • Peer Reviewed
• [Journal Article] Protein Crystallization in Agarose Gel with High Strength : Developing an Automated System for Protein Crystallographic Processes (075502) (2009)
  • Author(s): Shigeru Sugiyama, Kana Tanabe, Mika Hirose, Tomoya Kitatani, Hitoshi Hasenaka, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Tsuyoshi Inoue, Hiroyoshi Matsumura.
  • Journal Title: Jpn.J.Appl.Phys. 48 Pages: 1-5
  • Peer Reviewed
• [Journal Article] 多剤耐性 Multi-drug resistance (2009)
  • Author(s): 村上聡
  • Journal Title: Medical Science Digest 35 Pages: 346-347
  • Peer Reviewed
• [Journal Article] Femtosecond laser Processing of Agarose Gel Surrounding Protein Crystals for Development of an Automated Crystal Capturing System(105502) (2009)
  • Author(s): Shigeru Sugiyama, Hitoshi Hasenaka, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Hiroyoshi Matsumura
  • Journal Title: Jpn. J. Appl. Phys. 48 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Growth of Large Protein Crystals by Top-Seeded Solution Growth Together with the Floating and Soulution-Stirring Technique (2009)
  • Author(s): Noriko Shimizu, Shigeru Sugiyama, Mihoko Maruyama, Hiroshi Y.Yoshikawa, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue Hiroyoshi Matsumura, Yusuke Mori.
  • Journal Title: Cryst.Growth Des. 9 Pages: 5227-5232
  • Peer Reviewed
• [Journal Article] Promotion of Crystal Nucleation of Protein by Semi-Solid Agarose Gel (125501) (2009)
  • Author(s): Kana Tanabe, Mika Hirose, Ryota Murai, Shigeru Sugiyama, Noriko Shimizu, Mihoko Maruyama, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Eiichi Mizohata, Tsuyoshi Inoue, Hiroyoshi Matsumura.
  • Journal Title: Appl.Phys.Express 2 Pages: 1-3
  • Peer Reviewed
• [Journal Article] A Manipulating Tool for Protein Microcrystals in Solution Using Adhesive Materials (118001) (2009)
  • Author(s): Tomoya Kitatani, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano.
  • Journal Title: Jpn.J.Appl.Phys. 48 Pages: 1-2
  • Peer Reviewed
• [Journal Article] Femtosecond laser processing of protein crystals grown in agarose gel (2009)
  • Author(s): Hitoshi Hasenaka, Shigeru Sugiyama, Mika Hirose, Noriko Shimizu, Tomoya Kitatani, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Hiroyoshi Matsumura.
  • Journal Title: J.Cryst.Growth 312 Pages: 73-78
  • Peer Reviewed
• [Journal Article] Femtosecond laser-induced nucleation of protein in agalose gel (2009)
  • Author(s): H. Y. Yoshikawa, et al.
  • Journal Title: J. Cryst. Growth 311 Pages: 956-959
  • Peer Reviewed
• [Journal Article] Dynamic nature of disulphide bond formation catalysts revealed by crystal structures of DsbB. (2009)
  • Author(s): K. Inaba, et al.
  • Journal Title: EMBO J. 28 Pages: 779-791
  • Peer Reviewed
• [Journal Article] Evaluation and Improvement of a Technique to Manipulate Protein Crystals in Solution (2008)
  • Author(s): Tomoya Kitatani, Hiroaki Adachi, Shigeru Sugiyama, Hiroyoshi Matsumura, Ryota Murai, Yoshinori Takahashi, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano.
  • Journal Title: Jpn. J. Appl. Phys. 47 Pages: 8995-8997
  • NAID: 40016389649
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of Ca2+-free primary Ca2+-sensor of Na+/Ca2+ exchanger (2008)
  • Author(s): Masashi Mima, Chika Kawai, Kumsun Paku, Koji Tomoo, Toshimasa Ishida, Shigeru Sugiyama, Hiroshi Matsumura, Tomoya Kitatani, Hiroshi Y. Yoshikawa, Syou Maki, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Satomi Kita, TakahMasashi Mima
  • Journal Title: Acta Crystallogr F64 Pages: 1125-1127
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary neutron diffraction studies of HIV-1 protease cocrystallized with inhibitor KNI-272 (2008)
  • Author(s): Hiroshi Matsumura, Motoyasu Adachi, Shigeru Sugiyama, Shino Okada, Megumi Yamakami, Taro Tamada, Koushi Hidaka, Yoshio Hayashi, Tooru Kimura, Yoshiaki Kiso, Tomoya Kitatani, Syou. Maki, Hiroshi Y. Yoshikawa, Hiroaki Adachi, Kazufumi Takano, Satoshi MurakaHiroshi Matsumura, Motoyasu Adachi, Shigeru Su
  • Journal Title: Acta Crystallogr. F64 Pages: 1003-1006
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray diffraction studies of an RNA aptamer in complex with the human IgG Fc fragment (2008)
  • Author(s): Shigeru Sugiyama, Yusuke Nomura, Taiichi Sakamoto, Tomoya Kitatani, Asako Kobayashi, Shin Miyakawa, Yoshinori Takahashi, Hiroaki Adachi, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Yoshikazu Nakamura, Hiroyoshi Matsumura.
  • Journal Title: Acta Crystallogr. F64 Pages: 942-944
  • Peer Reviewed
• [Journal Article] Functional role of transmembrane Helix 6 in drug binding and transport by the ABC transporter MsbA (2008)
  • Author(s): Barbara Woebking, Saroj Velamakanni, Luca Federici, Markus A. Seeger, Satoshi Murakami, Hendrik van Veen.
  • Journal Title: Biochemistry. 47 Pages: 10904-10914
  • Peer Reviewed
• [Journal Article] Multidrug efflux transporter, AcrB-the pumping mechanism (2008)
  • Author(s): Satoshi Murakami
  • Journal Title: Curr. Opin. Struct. Biol 18 Pages: 459-465
  • Peer Reviewed
• [Journal Article] Laser energy dependence on femtosecond laser-induced nucleation of protein (2008)
  • Author(s): Hiroshi Y. Yoshikawa, Ryota Murai, Syou Maki, Tomoya Kitatani, Shigeru Sugiyama, Gen Sazaki, Hiroaki Adachi, Tsuyoshi Inoue, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Takatomo Sasaki, Yusuke Mori.
  • Journal Title: Appl. Phys. A 93 Pages: 911-915
  • Peer Reviewed
• [Journal Article] Effect of solution flow by rotary shaker on protein crystallization (2008)
  • Author(s): Ryota Murai, Hiroshi Y. Yoshikawa, Hisato Kawahara, Syou Maki, Shigeru Sugiyama, Tomoya Kitatani, Hiroaki Adachi, Kazufumi Takano, Hiroyoshi Matsumura, Satoshi Murakami, Tsuyoshi Inoue, Takatomo Sasaki, Yusuke Mori.
  • Journal Title: J. Cryst. Growth 310 Pages: 2168-2172
  • Peer Reviewed
• [Journal Article] Protein crystallization in a 100 nl solution with new stirring equipment (2008)
  • Author(s): Syou Maki, Ryota Murai, Hiroshi Y. Yoshikawa, Tomoya Kitatani, Shinya Nakata, Hisato Kawahara, Hitoshi Hasenaka, Asako Kobayashi, Shino Okada, Shigeru Sugiyama, Hiroaki Adachi, Hiroshi Matsumura, Kazufumi Takano, Satoshi Murakami, Tsuyoshi Inoue, TakatomoSyou Maki, Ryota Mura
  • Journal Title: J. Synchrotron Rad. 15 Pages: 269-272
  • Peer Reviewed
• [Journal Article] New Technique of Manipulating a Protein Crystal Using Adhesive Material (2008)
  • Author(s): Tomoya Kitatani, Shigeru Sugiyama, Hiroyoshi Matsumura, Hiroaki Adachi, Hiroshi Y. Yoshikawa, Syou Maki, Satoshi Murakami, Tsuyoshi Inoue, Yusuke Mori, Kazufumi Takano.
  • Journal Title: Appl. Phys. Express 1 Pages: 1-3
  • NAID: 10025079731
  • Peer Reviewed
• [Journal Article] ultidrug recognition and pumping by bacterial multidrug transporter ~ A structural view (2008)
  • Author(s): Satoshi Murakami
  • Journal Title: Protein Science 17 Pages: 168-168
  • Peer Reviewed
• [Journal Article] 多剤耐性の仕組み (2008)
  • Author(s): 村上聡
  • Journal Title: 日本臨床 66 Pages: 193-203
• [Journal Article] Effect of solution flow by rotary shaker on protein crystallization (2008)
  • Author(s): R. Murai, et al.
  • Journal Title: J. Cryst. Growth 310 Pages: 2168-2172
  • Peer Reviewed
• [Journal Article] Protein crystallization in a 100 nl solution with new stirring equipment (2008)
  • Author(s): S. Maki, et al.
  • Journal Title: J. Synchrotron Rad. 15 Pages: 269-272
  • Peer Reviewed
• [Journal Article] Multidrug efflux transporter, AcrB-the pumping mechanism (2008)
  • Author(s): S. Murakami
  • Journal Title: Curr. Opin. Struct. Biol 18 Pages: 459-465
  • Peer Reviewed
• [Journal Article] Laser energy dependence on femtosecond laser-induced nucleation of protein (2008)
  • Author(s): H. Y. Yoshikawa, et al.
  • Journal Title: Appl. Phys. A 93 Pages: 911-915
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray diffraction studies of an RNA aptamer in complex with the human IgG Fc fragment (2008)
  • Author(s): S. Sugiyama, et al.
  • Journal Title: Acta Crystallogr. F64 Pages: 942-944
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary neutron diffraction studies of HIV-1 protease cocrystallized with inhibitor KNI-272 (2008)
  • Author(s): H. Matsumura, et al.
  • Journal Title: Acta Crystallogr. F64 Pages: 1003-1006
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of Ca2+-free primary Ca2+-sensor of Na+/Ca2+exchanger (2008)
  • Author(s): M. Mima, et al.
  • Journal Title: Acta Crystallogr. F64 Pages: 1125-1127
  • Peer Reviewed
• [Journal Article] Evaluation and Improvement of a Technique to Manipulate Protein Crystals in Solution (2008)
  • Author(s): T. Kitatani, et al.
  • Journal Title: Jpn. J. Appl. Phys. 47 Pages: 8995-8997
  • Peer Reviewed
• [Journal Article] Crystal structures of a bacterial multidrug transporter reveal a functionally rotating mechanism. (2007)
  • Author(s): Satoshi Murakami, Akihito Yamaguchi
  • Journal Title: FEBS Journa 274Sup.1 Pages: 119-119
  • Peer Reviewed
• [Journal Article] 多剤排出トランスポーターの構造と機能結晶構造から明らかになった機能的回転メカニズム (2007)
  • Author(s): 村上聡
  • Journal Title: 蛋白質核酸酵素 52 Pages: 406-414
  • NAID: 40015447929
  • Peer Reviewed
• [Journal Article] 多剤排出トランスポーターの結晶構造解析により明らかになった機能的回転メカニズム (2007)
  • Author(s): 村上 聡
  • Journal Title: 生物物理 47 Pages: 309-316
  • NAID: 110006418741
• [Journal Article] 多剤排出トランスポーターの構造と機能 (2007)
  • Author(s): 村上 聡
  • Journal Title: 表面科学 28 Pages: 184-191
  • NAID: 10019506734
• [Journal Article] Crystal structures of a bacterial multidrug transporter reveal a functionally rotating mechanism (2007)
  • Author(s): Satoshi Murakami
  • Journal Title: the FEBS Journal 274 Pages: 119-119
  • Peer Reviewed
• [Journal Article] 大腸菌の異物排出トランスポーターAcrBの構造と異物認識機構 (2007)
  • Author(s): 村上 聡、山口 明人
  • Journal Title: 生化学 79 Pages: 542-549
• [Journal Article] 多剤排出トランスポーターの構造と機能 結晶構造から明らかになった機能的回転メカニズム (2007)
  • Author(s): 村上 聡
  • Journal Title: 蛋白質核酸酵素 52 Pages: 406-414
  • NAID: 40015447929
• [Presentation] 細菌の薬剤耐性化機構の結晶学的解析Mechanism of bacterial multi-drug resistance based on the crystal structure of efflux transporter (2010)
  • Author(s): 村上聡
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2010-03-28
• [Presentation] RND transporter, AcrB-The pumping mechanism (2010)
  • Author(s): Satoshi Murakami
  • Organizer: 3rd FEBS Special Meeting 『ATP-Binding Cassette Protein : Form Multidrug Resistance to Genetic Diseases』
  • Place of Presentation: Hotel Grauer Bar(オーストリア・インスブルック)
  • Year and Date: 2010-03-02
• [Presentation] Structure and Function of Multi-drug Transporter AcrB (2010)
  • Author(s): Satoshi Murakami
  • Organizer: 4th INTERNATIONAL SYMPOSIUM ON RECENT TRENDS IN MACROMOLECULAR STRUCTURE AND FUNCTION
  • Place of Presentation: マドラス大学(インド・チェンナイ)
  • Year and Date: 2010-01-22
• [Presentation] 細菌の薬剤耐性化機構の結晶学的解析Mechanism of bacterial multi-drug resistance based on the crystal structure of efflux transporter (2010)
  • Author(s): 村上聡
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] RNDtransporter, AcrB-Thepumping mechanism (2010)
  • Author(s): Satoshi Murakami
  • Organizer: 3rd FEBS Special Meeting『ATP-Binding Cassette Protein:From Multidrug Resistance to Genetic Diseases』
  • Place of Presentation: オーストリア
• [Presentation] Structure and Function of Multi-drug Transporter AcrB (2010)
  • Author(s): Satoshi Murakami
  • Organizer: 4th INTERNATIONAL SYMPOSIUM ON RECENT TRENDS IN MACROMOLECULAR STRUCTURE AND FUNCTION
  • Place of Presentation: インド
• [Presentation] Structure and Function of the Drug EffluxTransporter (2009)
  • Author(s): Satoshi Murakami
  • Organizer: 第一回台湾膜タンパク質・生物膜結晶と構造生物学シンポジウム
  • Place of Presentation: 国立台湾大学(台湾・台北)
  • Year and Date: 2009-12-10
• [Presentation] Multidrug Efflux Transporter -The Pumping Mechanism (2009)
  • Author(s): Satoshi Murakami
  • Organizer: Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes
  • Place of Presentation: 京都大学医学部 芝蘭会館(京都)
  • Year and Date: 2009-09-08
• [Presentation] Multidrug Efflux Transporter -The Pumping Mechanism (2009)
  • Author(s): Satoshi Murakami
  • Organizer: 1st International Multidisciplinary Workshop 『Membrane Biology Frontiers -Dynamics, Energy, Structure and Technology-』
  • Place of Presentation: Royal Myconian Hotel, Elia Beach, Mykonos, Greece(ギリシャ・ミコノス)
  • Year and Date: 2009-05-30
• [Presentation] Structure and Function of the Drug EffluxTransporter (2009)
  • Author(s): Satoshi Murakami
  • Organizer: 第一回台湾膜タンパク質・生物膜結晶構造生物学シンポジウム
  • Place of Presentation: 台湾
• [Presentation] Multidrug Efflux Transporter-The Pumping Mechanism (2009)
  • Author(s): Satoshi Murakami
  • Organizer: 1st International Multidisciplinary Workshop『Membrane Biology Frontiers-Dynamics, Energy, Structure and Technology-』
  • Place of Presentation: ギリシャ
• [Presentation] Transport by AcrB;a Structual approach (2009)
  • Author(s): Satoshi Murakami
  • Organizer: Gordon Reseach Conferences
  • Place of Presentation: アメリカ
• [Presentation] 多剤排出輸送体の結晶構造に基づく多基質認識と輸送メカニズム (2009)
  • Author(s): 村上聡
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Transport by AcrB; a structural approach. (2009)
  • Author(s): S. Murakami
  • Organizer: The Gordon Research Conference (Multi-Drug Efflux Systems)
  • Place of Presentation: Galveston, TX, USA
• [Presentation] 多剤排出輸送体の結晶構造に基づく多基質認識と輸送メカニズム (2009)
  • Author(s): 村上 聡
  • Organizer: 第82回日本薬理学会年会シンポジウム
  • Place of Presentation: パシフィコ横浜
• [Presentation] 高粘性溶液中でのフェムト秒レーザー誘起タンパク質結晶核発生 (2009)
  • Author(s): 村井良多ら
  • Organizer: 第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学筑波キャンパス(茨城県つくば市)
• [Presentation] フェムト秒レーザーを用いたタンパク質結晶加工 (2009)
  • Author(s): 長谷中仁志ら
  • Organizer: 第22回日本放射光学会年会
  • Place of Presentation: 東京大学本郷キャンパス
• [Presentation] 多剤排出トランスポーターによる多基質認識とその排出メカニズム (2008)
  • Author(s): 村上聡
  • Organizer: 第7回微生物研究会微生物研究会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-14
• [Presentation] 細菌排出システムの構造機能解析 (2008)
  • Author(s): ○村上 聡
  • Organizer: 第81回日本細菌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-03-25
• [Presentation] Structure and mechanism of bacterial multi-drug efflux systems (2008)
  • Author(s): ○Satoshi Murakami
  • Organizer: Gordon Research Conferences (NEW ANTIBACTERIAL DISCOVERY & DEVELOPMENT)
  • Place of Presentation: II Ciocco Hotel and Resort Lucca (Barga), Italy
  • Year and Date: 2008-03-12
• [Presentation] 多剤排出トランスポーターによる薬剤排出機構 (2008)
  • Author(s): ○村上 聡
  • Organizer: 千里ライフサイエンスセミナー 「生命機能を支える生体超分子の高次構造と機能」
  • Place of Presentation: 大阪
  • Year and Date: 2008-02-28
• [Presentation] Multidrug recognition and pumping by bacterial multidrug transporter-A structural view (2008)
  • Author(s): ○Satoshi Murakami
  • Organizer: GPCRシンポジウム-膜タンパク質結晶構造解析のフロンティア-
  • Place of Presentation: 兵庫
  • Year and Date: 2008-02-26
• [Presentation] Multidrug recognition and pumping by bacterial multidrug efflux transporter-A structural view (2008)
  • Author(s): ○Satoshi Murakami
  • Organizer: Katzir Workshop on The Unbearable Complexity of Life
  • Place of Presentation: Tel Aviv University, Israel
  • Year and Date: 2008-02-11
• [Presentation] Multidrug recognition and pumping by bacterial multidrug efflux transporter-A structural view (2008)
  • Author(s): ○Satoshi Murakami
  • Organizer: International Symposium on Recent Trends in Macromolecular Structure and Function
  • Place of Presentation: Chennai, India
  • Year and Date: 2008-01-07
• [Presentation] 大腸菌多剤排出トランスポーターによる薬剤認識および排出機構 (2008)
  • Author(s): 村上聡
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡
• [Presentation] Bacterial multi drug efflux transporter AcrB, -The pumping mechanism (2008)
  • Author(s): Satoshi Murakami
  • Organizer: XXI Congress of the International Union of Crystallography
  • Place of Presentation: 大阪
• [Presentation] Multidrug Recognition and Pumping by Bacterial Multidrug Transporter-A structual View (2008)
  • Author(s): Satoshi Murakami
  • Organizer: 22nd Annual Symposium of The Protein Society
  • Place of Presentation: アメリカ
• [Presentation] 大腸菌多剤排出トランスポーターによる薬剤認識および排出機構 (2008)
  • Author(s): 村上聡
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京
• [Presentation] Protein Crystallization by Femtosecond Laser Irradiation and Solution Stirring (2008)
  • Author(s): K. Takano, et al.
  • Organizer: The 4th Asian Conference on Crystal Growth and Crystal Technology (CGCT-4)
  • Place of Presentation: 仙台
• [Presentation] 大腸菌多剤排出トランスポーターによる薬剤認識および排出機構 (2008)
  • Author(s): 村上 聡
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] Multidrug Recognition and Pumping by Bacterial Multidrug Transporter -A Structural View (2008)
  • Author(s): S. Murakami
  • Organizer: 22nd Annual Symposium of The Protein Society
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Protein Crystallization by Femtosecond Laser Ablation in Supersaturated Solution (2008)
  • Author(s): H. Y. Yoshikawa, et al.
  • Organizer: IUPAC Symposium on Photochemistry
  • Place of Presentation: Gothenburg, Sweden
• [Presentation] Bacterial Multidrug Efflux Transporter AcrB - The pumping mechanism. (Micro Symposium on New membrane protein structure. ) (2008)
  • Author(s): S. Murakami
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] 大腸菌多剤排出トランスポーターによる薬剤認識および排出機構 (2008)
  • Author(s): 村上 聡
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場(福岡市博多区)
• [Presentation] Development of a Novel Mounting Method for Protein Crystals (2008)
  • Author(s): T. Kitatani, et al.
  • Organizer: The 4th Asian Conference on Crystal Growth and Crystal Technology (CGCT-4)
  • Place of Presentation: 仙台
• [Presentation] Growth of large protein crystals by hanging a seed crystal (CGCT-4) (2008)
  • Author(s): N. Shimizu, et al.
  • Organizer: The 4th Asian Conference on Crystal Growth and Crystal Technology
  • Place of Presentation: 仙台
• [Presentation] 膜タンパク質の結晶育成〜膜輸送体の結晶構造 (2008)
  • Author(s): 村上 聡
  • Organizer: 第7回国際バイオフォーラム
  • Place of Presentation: 東京ビッグサイト
• [Presentation] フェムト秒レーザー照射によるタンパク質核発生メカニズムの解明と最適条件の検討 (2008)
  • Author(s): 村井良多ら
  • Organizer: 第38回結晶成長国内会議
  • Place of Presentation: 仙台市戦災復興記念館(宮城県仙台市)
• [Presentation] New Technique of Manipulating a Protein Crystal (2008)
  • Author(s): K. Takano, et al.
  • Organizer: The 2008 Annual Meeting of the American Crystallographic Association (ACA2008)
  • Place of Presentation: Knoxville Convention Center, Knoxville, Tennessee, USA
• [Presentation] フェムト秒レーザー照射によるタンパク質結晶化の原理解明研究 (2008)
  • Author(s): 村井良多ら
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: タワーホール船堀
• [Presentation] Study on Nucleation Mechanism with Femtosecond Laser Irradiation (2008)
  • Author(s): R. Murai, et al.
  • Organizer: 1st Global COE Student Conference on Innovative Electronic Topics SCIENT2008
  • Place of Presentation: 大阪大学
• [Presentation] Crystal structure of RNA aptamer in complex with human immunoglobulin G (2008)
  • Author(s): H. Matsumura, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] Growth of high-quality and large crystals of HIV protease-in-hibitor complex for neutron crystallography (2008)
  • Author(s): M. Yamakami, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] Crystallization and preliminary X-ray diffraction studies of RNA aptamer in complex with human immunoglobulin G. (2008)
  • Author(s): S. Sugiyama, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] Study on femtosecond laser-induced nucleation dynamics of proteins (2008)
  • Author(s): R. Murai, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] Wavelength dependence of the crystallization by the laser irradiation (2008)
  • Author(s): H. Hasenaka, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グラシキューブ大阪
• [Presentation] The first direct observation of individual protein molecules on a protein crystal under forced solution-flow (2008)
  • Author(s): H. Kawahara, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] Growth of large protein crystals for neutron crystallography by hanging a seed crystal (2008)
  • Author(s): N. Shimizu, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] Application of a novel mounting tool using adhesive for protein crystals (2008)
  • Author(s): T. Kitatani, et al.
  • Organizer: XXI Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: グランキューブ大阪
• [Presentation] X-ray structure of RNA aptamer in complex with human immunoglobulin G (2008)
  • Author(s): S. Sugiyama, et al.
  • Organizer: 第46回日本生物物理学会
  • Place of Presentation: 福岡国際会議場(福岡市博多区)
• [Presentation] 多剤排出トランスポーターによる多基質認識と輸送機耕を考える (2007)
  • Author(s): ○村上 聡
  • Organizer: 第45回生物物理学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-23
• [Presentation] Molecular mechanism of multidrug efflux transporter (2007)
  • Author(s): ○村上 聡
  • Organizer: 第30回日本分子生物学会年会・第80回日本生化学会大会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-11
• [Presentation] 膜輸送体作動メカニズムの結晶学的解明 (2007)
  • Author(s): ○村上 聡、中島 良介、松本 崇、近藤 洋平、津田 岳夫、小川 治夫、豊島 近
  • Organizer: 第11回 Spring8シンポジウム
  • Place of Presentation: 兵庫
  • Year and Date: 2007-10-29
• [Presentation] 膜タンパク質を含むターゲット蛋白質の精製 (2007)
  • Author(s): 村上聡
  • Organizer: 千里ライフサイエンス技術講習会第47回「蛋白質X線構造解析技術～発現からデータ処理まで～」
  • Place of Presentation: 大阪
  • Year and Date: 2007-10-23
• [Presentation] 膜タンパク質を含むターゲット蛋白質の精製 (2007)
  • Author(s): ○村上 聡
  • Organizer: 千里ライフサイエンス技術講習会 第47回 「蛋白質X線構造解析技術 〜発現からデータ処理まで〜」
  • Place of Presentation: 大阪
  • Year and Date: 2007-10-23
• [Presentation] Structural aspects of drug recognition and transport by bacterial multidrug efflux transporter (2007)
  • Author(s): ○村上 聡
  • Organizer: 財団法人 応用微生物学研究奨励会設立50周年記念東京大学創立130周年記念 第12回分生研シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2007-10-11
• [Presentation] 多剤排出トランスポーターの結晶構造と薬剤排出メカニズム (2007)
  • Author(s): ○村上 聡
  • Organizer: サントリー生有研フォーラム 小分子を運ぶトランスポーター機能制御と機構解明に向けて
  • Place of Presentation: 京都
  • Year and Date: 2007-08-31
• [Presentation] Structure and mechanism of bacterial multi-drug efflux transporter (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: 24th European Crystallographic Meeting
  • Place of Presentation: Marrakech, Morocco
  • Year and Date: 2007-08-22
• [Presentation] Structure and mechanism of AcrAB bacterial multi-drug efflux pump (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: Seminar
  • Place of Presentation: Imperial College London, London, England
  • Year and Date: 2007-08-21
• [Presentation] Structure and mechanism of AcrAB bacterial multi-drug efflux pump (2007)
  • Author(s): Satoshi Murakami
  • Organizer: AstraZeneca Membran Protein Day(Seminar)
  • Place of Presentation: イングランド
  • Year and Date: 2007-08-20
• [Presentation] Structure and mechanism of AcrAB bacterial multi-drug efflux pump (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: AstraZeneca Membran Protein Day(Seminar)
  • Place of Presentation: Alderlay Park, Macclesfield, UK
  • Year and Date: 2007-08-20
• [Presentation] Structural studies of multi-drug efflux transporter (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: 9th International Conference on Biology and Synchrotron Radiation
  • Place of Presentation: Manchester, England
  • Year and Date: 2007-08-15
• [Presentation] 多剤排出トランスポーターによる薬剤認識および排出機構 (2007)
  • Author(s): ○村上 聡
  • Organizer: 日本ヒトプロテオーム機構第5回大会〜プロテオミクス生物学から医科学研究へ向かう新たな潮流〜
  • Place of Presentation: 日本科学未来館、東京
  • Year and Date: 2007-07-31
• [Presentation] 膜タンパク質精製への新しいアプローチ (2007)
  • Author(s): ○村上 聡
  • Organizer: GE Healthcare, Challenging Protein Purification Seminar
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-19
• [Presentation] 膜タンパク質精製への新しいアプローチ (2007)
  • Author(s): ○村上 聡
  • Organizer: GE Healthcare, Challenging Protein Purification Seminar
  • Place of Presentation: 東京
  • Year and Date: 2007-07-19
• [Presentation] Crystal structures of a bacterial multidrug transporter reveal a function ally rotating mechanism (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: 32nd FEBS Congress, Molecular Machines
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2007-07-08
• [Presentation] 多剤排出トランスポーターの結晶構造に基づく機能解明 (2007)
  • Author(s): ○村上 聡
  • Organizer: 第34回 生体分子科学討論会
  • Place of Presentation: 仙台
  • Year and Date: 2007-06-22
• [Presentation] 膜タンパク質の結晶育成〜膜輸送体の結晶構造 (2007)
  • Author(s): ○村上 聡
  • Organizer: 第6回国際バイオフォーラム
  • Place of Presentation: 東京
  • Year and Date: 2007-06-20
• [Presentation] Structure and mechanism of AcrAB bacterial multi-drug efflux pump (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: Seminar
  • Place of Presentation: Scripps Inst., SanDiego
  • Year and Date: 2007-05-25
• [Presentation] Structure and mechanism of AcrAB bacterial multi-drug efflux pump (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: Seminar
  • Place of Presentation: TakedaSD, SanDiego
  • Year and Date: 2007-05-24
• [Presentation] Structure and mechanism of AcrAB bacterial multi-drug efflux pump (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: Seminar
  • Place of Presentation: Mpex Pharmaceuticals, Inc., SanDiego
  • Year and Date: 2007-05-23
• [Presentation] Structure and mechanism of AcrAB bacterial multi-drug efflux pump (2007)
  • Author(s): ○Satoshi Murakami
  • Organizer: Seminar
  • Place of Presentation: UCSF, USA
  • Year and Date: 2007-05-22
• [Presentation] 膜輸送体作動メカニズムの結晶学的解明 (2007)
  • Author(s): 村上聡、中島良介、松本崇、近藤洋平1、津田岳夫1、小川治夫1、豊島近1
  • Organizer: 第11回Spring8シンポジウム
  • Place of Presentation: 兵庫
• [Presentation] Structure and mechanism of bacterial multi-drug efflux transporter (2007)
  • Author(s): Satoshi Murakami
  • Organizer: 24th European Crystallographic Meeting
  • Place of Presentation: モロッコ
• [Presentation] Session1 Multi-Protein complex, Structural studies of multi-drug efflux transporter (2007)
  • Author(s): Satoshi Murakami
  • Organizer: 9th International Conference on Biology and Synchrotron Radiation
  • Place of Presentation: イングランド
• [Presentation] Crystal structures of a bacterial multidrug transporter reveal a functionally rotating mechanism (2007)
  • Author(s): Satoshi Murakami
  • Organizer: 32nd FEBS Congress, Molecular Machines
  • Place of Presentation: オーストリア
• [Book] やさしい原理から入るタンパク質科学実験:Iタンパク質をつくる-抽出・精製と合成 (2008)
  • Author(s): 村上聡
  • Publisher: 化学同人
• [Book] シリーズ生物薬科学実験講座 (2008)
  • Author(s): 村上聡
  • Publisher: 廣川書店
• [Book] 多剤耐性のしくみ (2008)
  • Author(s): 村上 聡
  • Total Pages: 11
  • Publisher: 日本臨床
• [Book] やさしい原理からはいるタンパク質科学実験法 (2008)
  • Author(s): 村上 聡
  • Total Pages: 27
  • Publisher: 化学同人
• [Book] 生化学vol. 79 (2007)
  • Author(s): 村上聡
  • Publisher: 日本生化学会
• [Patent(Industrial Property Rights)] 有機高分子結晶製造装置 (2009)
  • Inventor(s): 杉山成、安達宏昭、松村浩由、高野和文、村上聡、井上豪、森勇介、国宗範彰
  • Industrial Property Rights Holder: 株式会社クニムネ
  • Industrial Property Number: 2009-275465
  • Filing Date: 2009-12-03
• [Patent(Industrial Property Rights)] 結晶製造方法、凍結結晶製造方法、結晶構造解析方法、結晶化スクリーニング方法、結晶化スクリーニング装置 (2008)
  • Inventor(s): 杉山成、安達宏昭、松村浩由、高野和文、村上聡、井上豪、森勇介
  • Industrial Property Rights Holder: 株式会社創晶(506243161)
  • Industrial Property Number: 2008-008561
  • Filing Date: 2008-01-17
• [Patent(Industrial Property Rights)] 粘着式ピンセット、粘着式ピンセット用部材、粒子の保持方法、粒子の解析方法および分析装置 (2007)
  • Inventor(s): 安達宏昭、松村浩由、高野和文、村上聡、井上豪、森勇介、柴田和博、上野元信、山本浩司
  • Industrial Property Rights Holder: 有限会社シバタシステムサービス
  • Industrial Property Number: 2007-296354
  • Filing Date: 2007-11-15
• [Patent(Industrial Property Rights)] 有機高分子結晶製造装置
  • Inventor(s): 杉山成、安達宏昭、松村浩由、高野和文、村上聡、井上豪、森勇介、国宗範彰
  • Industrial Property Rights Holder: 株式会社クニムネ
  • Industrial Property Number: 2009-275465