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Elucidation of speciation process of amphidiploid species in Brassica

Research Project

Project/Area Number 19208001
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionTohoku University

Principal Investigator

NISHIO Takeshi  Tohoku University, 大学院・農学研究科, 教授 (30301039)

Co-Investigator(Kenkyū-buntansha) KITASHIBA Hiroyasu  東北大学, 大学院・農学研究科, 准教授 (80431542)
TAKAHATA Yoshihito  岩手大学, 農学部, 教授 (10133894)
YOKOI Shuji  岩手大学, 農学部, 准教授 (80346311)
KANEKO Yukio  宇都宮大学, 農学部, 教授 (40241848)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥49,530,000 (Direct Cost: ¥38,100,000、Indirect Cost: ¥11,430,000)
Fiscal Year 2009: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2007: ¥20,930,000 (Direct Cost: ¥16,100,000、Indirect Cost: ¥4,830,000)
Keywords種間不和合性 / 雑種胚崩壊 / 自家不和合性 / ゲノム再配列 / 遺伝子発現制御 / 遺伝子発現解析
Research Abstract

Cooperative research of three laboratories was performed to elucidate the speciation process of amphidiploid species in genus Brassica by investigating genes responsible for interspecific incompatibility and breakdown of hybrid embryos and mechanisms of self-compatibility. We identified QTLs of the strengths of interspecific incompatibility and embryo breakdown, which are clues to identification of the genes. Several S genotypes were found in three amphidiploid species, and mutations of dominant S haplotypes were considered to be the cause of self-compatibility.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (53 results)

All 2010 2009 2008 2007

All Journal Article (29 results) (of which Peer Reviewed: 29 results) Presentation (18 results) Book (6 results)

  • [Journal Article] Improvement of the dot-blot-SNP technique for efficient and cost-effective genotyping2010

    • Author(s)
      Shiokai S, Shirasawa K, Sato Y, Nishio T
    • Journal Title

      Mol.Breed. 25

      Pages: 179-186

    • NAID

      120006581078

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of genetic diversity of accessions in Brassicaceae genetic resources by frequency distribution analysis of S haplotypes2010

    • Author(s)
      Takuno S, Oikawa E, Kitashiba H, Nishio T
    • Journal Title

      Theor.Appl.Genet. 120

      Pages: 1129-1138

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic analysis of interspecific incompatibility in Brassica rapa2010

    • Author(s)
      Udagawa H, Ishimaru Y, Li F, Sato Y, Kitashiba H, Nishio T
    • Journal Title

      Theor.Appl.Genet. (印刷中)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis2010

    • Author(s)
      Oikawa E, Takuno S, Izumita A, Sakamoto K, Hanzawa H, Kitashiba H, Nishio T
    • Journal Title

      Mol.Breed. (印刷中)

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of the optimum hybridization conditions of dot-blot-SNP analysis using estimated melting temperature of oligonucleotide probes2010

    • Author(s)
      Shiokai S, Kitashiba H, Nishio T
    • Journal Title

      Plant Cell Rep. (印刷中)

    • NAID

      120006581079

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of the dot-blot-SNP technique for efficient and cost-effective genotyping2010

    • Author(s)
      Shiokai S, Shirasawa K, Sato Y, Nishio T
    • Journal Title

      Molecular Breeding 25

      Pages: 179-186

    • NAID

      120006581078

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assessment of genetic diversity of accessions in Brassicaceae genetic resources by frequency distribution analysis of S haplotypes.2010

    • Author(s)
      Takuno S, Oikawa E, Kitashiba H. Nishio T
    • Journal Title

      Theoretical and Applied Genetics 120

      Pages: 1129-1138

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic analysis of interspecific incompatibility in Brassica rapa.2010

    • Author(s)
      Udagawa H, Ishimaru Y, Li F, Sato Y, Kitashiba H, Nishio T
    • Journal Title

      Theoretical and Applied Genetics (未定, 印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits2009

    • Author(s)
      Li F, Kitashiba H, Inaba K, Nishio T
    • Journal Title

      DNA Res. 16

      Pages: 311-323

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Leaf-punch method to prepare a large number of PCR templates from plants for SNP analysis2009

    • Author(s)
      Shiokai S, Kitashiba H, Shirasawa K, Nagano K, Nishio T
    • Journal Title

      Mol.Breed. 23

      Pages: 329-336

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploids "Raphanobrassica"2009

    • Author(s)
      Akaba M, Kaneko Y, Ito Y, Nakata Y, Bang BW, Matsuzawa Y
    • Journal Title

      Breed.Sci. 59

      Pages: 109-118

    • NAID

      10025922735

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line2009

    • Author(s)
      Akaba M, Kaneko Y, Hatakeyama K, Ishida M, Bang BW, Matsuzawa Y
    • Journal Title

      Breed.Sci. 59

      Pages: 203-206

    • NAID

      10025923032

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Brassica rapa linkage map of EST-based SNP markers for identification of candidate genes controlling flowering time and leaf morphological traits.2009

    • Author(s)
      Li F, Kitashiba H, Inaba K, Nishio T
    • Journal Title

      DNA Research 16

      Pages: 311-323

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic a mphidiploids "Raphanobrassica".2009

    • Author(s)
      Akaba M, Kaneko Y, Ito Y, Nakata Y, Bang BW, Matsuzawa Y
    • Journal Title

      Breeding Science 59

      Pages: 109-118

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus-Raphanus sativus monosomic addition line.2009

    • Author(s)
      Akaba M, Kaneko Y, Hatakeyama K, Ishida M, Bang BW. Matsuzawa Y
    • Journal Title

      Breeding Science 59

      Pages: 203-206

    • NAID

      10025923032

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Leaf-punch method to prepare a large number of PCR templates from plants for SNP analysis2009

    • Author(s)
      Shiokai S, Kitashiba H, Shirasawa K, Nagano K, Nishio T
    • Journal Title

      Mol. Breeding 23

      Pages: 329-336

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidipoid "Raphanobrassica"2009

    • Author(s)
      Akaba M, Kaneko Y, Ito Y, Nakata Y, Bang S-W, Mats uzawa Y
    • Journal Title

      Breed. Sci 59(印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preservation of a pseudogene by gene conversion and diversifying selection2008

    • Author(s)
      Takuno S, Nishio T, Satta Y, Innan H
    • Journal Title

      Genetics 180

      Pages: 517-531

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypomethylation and transcriptional reactivation of retrotransposon-like sequences in ddm1 transgenic plants of Brassica rapa2008

    • Author(s)
      Fujimoto R, Sasaki T, Inoue H, Nishio T
    • Journal Title

      Plant Mol.Biol. 66

      Pages: 463-473

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae species2008

    • Author(s)
      Fujimoto R, Takuno S, Sasaki T, Nishio T
    • Journal Title

      Genes Genet.Syst. 83

      Pages: 13-22

    • NAID

      10024395355

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genotypic variation for floral characters in Brassica and allied genera with special reference to breeding system2008

    • Author(s)
      Takahata Y, Konno N, Hinata K
    • Journal Title

      Breed.Sci. 58

      Pages: 385-392

    • NAID

      110007007907

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preservation of a pseudogene by gene conversion and diversifying selection2008

    • Author(s)
      Takuno S, Nishio T, Satta Y, Innan H.
    • Journal Title

      Genetics 180

      Pages: 517-531

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotypic variation for floral characters in Brassica and allied genera with special reference to breeding system2008

    • Author(s)
      Takahata, Y., N. Konnno and K. Hinata
    • Journal Title

      Breed. Sci 58

      Pages: 385-392

    • NAID

      110007007907

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hypomethylation and transcriptional reactivation of retrotransposon-like sequences in ddm1 transgenic plants of Brassica rapa.2008

    • Author(s)
      Fujimoto R, Sasaki T, Inoue H, Nishio T
    • Journal Title

      Plant Molecular Biology 66

      Pages: 463-473

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The pattern of amplification and differentiation of Ty1-copia and Ty3-gypsy retrotransposons in Brassicaceae species.2008

    • Author(s)
      Fujimoto R, Takuno S, Sasaki T, Nishio T
    • Journal Title

      Genes Genetic Systems 83

      Pages: 13-22

    • NAID

      10024395355

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of recombination on the hitchhiking diversity in Brassica self-incompatibility locus complex2007

    • Author(s)
      Takuno S, Fujimoto R, Sugimura T, Sato K, Okamoto S, Zhang S-L, Nishio T
    • Journal Title

      Genetics 177

      Pages: 949-958

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Self-compatibility in Brassica napus is caused by independent mutations in S-locus genes2007

    • Author(s)
      Okamoto S, Odashima M, Fujimoto R, Sato Y, Kitashiba H, Nishio T
    • Journal Title

      Plant J. 50

      Pages: 391-400

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Self-compatibility in Brassica napus is caused by independent mutations in S-locus genes.2007

    • Author(s)
      Okamoto S, Odashima M, Fujimoto R, Sato Y, Kitashiba H, Nishio T
    • Journal Title

      Plant Journal 50

      Pages: 391-400

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of recombination on the hitchhiking diversity in Brassica self-incompatibility locus complex.2007

    • Author(s)
      Takuno S, Fujimoto R, Sugimura T, Sato K, Okamoto S, Zhang S-L, Nishio T
    • Journal Title

      Genetics 177

      Pages: 949-958

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Brassica rapa と Raphanus sativus の属間交雑における雑種胚崩壊性のQTL解析2010

    • Author(s)
      殿崎薫・道場和也・北柴大泰・房相佑・金子幸雄・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-27
    • Related Report
      2009 Final Research Report
  • [Presentation] Brassica napusとその両親種(B.rapa、B.oleracea)間の雑種胚珠発生時における遺伝子群の解析2010

    • Author(s)
      玉田深平・福島香・横井修司・高畑義人
    • Organizer
      日本育種学会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Brassica rapaとRaphanus sativusの属間交雑における雑種胚崩壊性のQTL解析2010

    • Author(s)
      殿崎薫・道場和也・北柴大泰・房相佑・金子幸雄・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Brassica rapa種間不和合性に関するQTL解析2009

    • Author(s)
      宇田川久史・石丸洋次・李鋒・佐藤豊・北柴大泰・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dot-blot法を用いたBrassica属Sハプロタイプ選抜技術の開発2009

    • Author(s)
      及川恵里子・北柴大泰・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Brassica rapaとArabidopsis thalianaの比較連鎖地図とその農業形質QTLにおける遺伝子推定への利用2009

    • Author(s)
      李鋒・北柴大泰・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Brassica rapa×Raphanus sativusから得たF1雑種集団の種子稔性2009

    • Author(s)
      道場和也・殿崎薫・房相佑・金子幸雄
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] B. rapa×R. sativusにおける高雑種形成能素材の開発2009

    • Author(s)
      殿崎薫・道場和也・房相佑・金子幸雄・松澤康男
    • Organizer
      日本育種学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Dot-blot法を利用したBrassica属Sハプロタイプ同定技術の改良2008

    • Author(s)
      及川英里子・宅野将平・泉田敦・榛澤英明・坂本浩司・北柴大泰・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] mur型細胞質雄性不稔Brassicca oleraceaの稔性回復遺伝子に連鎖する分子マーカーの同定2008

    • Author(s)
      品田智隆・稲葉清文・岸谷幸枝・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Construction of a linkage map of Brassica rapa based on dot-blot-SNP markers and QTL analysis for flowering time and leaf moroholonical traits2008

    • Author(s)
      Li F, Ishimaru Y, Kitashiba H, Nishio T
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Brassica napus cv. WestarにおけるSPII変異の相補による自家和合性の獲得2008

    • Author(s)
      栃木隆廣・岡本俊介・北柴大泰・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      滋賀県立大学
    • Year and Date
      2008-10-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Brassica rapaにおけるBrDDM1によるDNAメチル化制御の解析2008

    • Author(s)
      佐々木卓・藤本龍・岸谷幸枝・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      明治大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brassica rapa在来品種における自家不和合性対立遺伝子の頻度分布と優劣性の関係2008

    • Author(s)
      宅野将・及川英里子・北柴大泰・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      明治大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brassica napusとその両親種(B. rapa、 B. oleracea)間の交雑親和性差異の解析2008

    • Author(s)
      玉田深平・横井修司・高畑義人
    • Organizer
      日本育種学会
    • Place of Presentation
      明治大学
    • Year and Date
      2008-03-28
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brassica rapa系統C451における自家和合性原因の解析2007

    • Author(s)
      伊藤進・北柴大泰・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      山形大学
    • Year and Date
      2007-09-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brassica属自家不和合性複合遺伝子座における組換えの検出と塩基配列多型への影響2007

    • Author(s)
      宅野将平・藤本龍・杉村哲・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      山形大学
    • Year and Date
      2007-09-23
    • Related Report
      2007 Annual Research Report
  • [Presentation] Brassica rapaにおける器官特異的DNAメチル化の解析2007

    • Author(s)
      佐々木卓・藤本龍・岸谷幸枝・西尾剛
    • Organizer
      日本育種学会
    • Place of Presentation
      山形大学
    • Year and Date
      2007-09-23
    • Related Report
      2007 Annual Research Report
  • [Book] Self-incompatibility in Brassicaceae species. In "Biology and breeding of crucifers" Ed.2009

    • Author(s)
      Kitashiba H, Nishio T
    • Publisher
      By Gupta SK Taylor & Francis Group, LLC
    • Related Report
      2009 Final Research Report
  • [Book] Floral variation in the subtribe Brassicinae with special reference to pollination strategies and pollen-ovule ratios. In "Biology and breeding of crucifers" Ed.2009

    • Author(s)
      Takahata Y
    • Publisher
      By Gupta SK Taylor & Francis Group, LLC
    • Related Report
      2009 Final Research Report
  • [Book] Distant hybridization. In "Biology and breeding of crucifers" Ed.2009

    • Author(s)
      Kaneko Y, Bang S-W, Matsuzawa Y
    • Publisher
      By Gupta SK Taylor & Francis Group, LLC
    • Related Report
      2009 Final Research Report
  • [Book] Self-incompatibility in Brassicaceae species. In "Biology and breeding of crucifers"2009

    • Author(s)
      Kitashiba H. Nishio T
    • Publisher
      Taylor & Francis Groun, LLC
    • Related Report
      2008 Annual Research Report
  • [Book] Distant hybridization. In "Biology and breeding of crucifers"2009

    • Author(s)
      Kaneko Y, Bang S-W, Matsuzawa Y
    • Publisher
      Taylor & Francis Group, LLC
    • Related Report
      2008 Annual Research Report
  • [Book] Self-incompatibility. Adv.Bot.Res.2007

    • Author(s)
      Fujimoto R, Nishio T
    • Related Report
      2009 Final Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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