Molecular mechanism of transcription factor controlling plant immunity and its application in the production of pathogen-resistant plants.

• Project/Area Number: 19208004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Nagoya University
• Principal Investigator: YOSHIOKA Hirofumi Nagoya University, 生命農学研究科, 准教授 (30240245)
• Co-Investigator(Kenkyū-buntansha): 川北 一人 名古屋大学, 大学院・生命農学研究科, 教授 (90186065)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000); Fiscal Year 2010: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2009: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2007: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
• Keywords: シグナル伝達 / 植物免疫 / WRKY型転写因子 / 耐病性植物 / MAPキナーゼ / 植物病理学 / 植物 / 病理学 / 転写因子

Research Abstract

Mitogen-activated protein kinase (MAPK) cascades have pivotal roles in plant innate immunity. However, downstream signaling of plant defense-related MAPKs is not well understood. Here we provide evidence that Nicotiana benthamiana WRKY8 is a physiological substrate of SIPK, NTF4, and WIPK. Phosphorylation of WRKY8 increased its DNA-binding activity. MAPK-mediated phosphorylation of WRKY8 has an important role in the defense response through activation of downstream genes.

Research Products

• [Journal Article] Regulatory mechanisms of nitric oxide and reactive oxygen species generation and their role in plant immunity. (2011)
  • Author(s): Yoshioka, H., Mase, K., Yoshioka, M., Kobayashi, M., Asai, S.
  • Journal Title: Nitric Oxide
  • Peer Reviewed
• [Journal Article] Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. (2011)
  • Author(s): Ishihama, N., Yamada, R., Yoshioka, M., Katou, S., Yoshioka, H.
  • Journal Title: Plant Cell 23 Pages: 1153-1170
  • Peer Reviewed
• [Journal Article] A plastidic glucose-6-phosphate dehydrogenase is responsible for hypersensitive response cell death and reactive oxygen species production. (2011)
  • Author(s): Asai, S., Yoshioka, M., Nomura, H., Tone, C., Nakajima, K., Nakane, E., Doke, N., Yoshioka, H.
  • Journal Title: J.Gen.Plant Pathol. 77 Pages: 152-162
  • NAID: 10029721013
  • Peer Reviewed
• [Journal Article] A plastidic glucose-6-phosphate dehydrogenase is responsible for hypersensitive response cell death and reactive oxygen species production. (2011)
  • Author(s): Asai, S., Yoshioka, M., Nomura, H., Tone, C., Nakajima, K., Nakane, E., Doke, N., Yoshioka, H.
  • Journal Title: J. Gen. Plant Pathol. Volume: 77 Pages: 152-162
  • NAID: 10029721013
  • Peer Reviewed
• [Journal Article] Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. (2011)
  • Author(s): Ishihama, N., Yamada, R., Yoshioka, M., Katou, S., Yoshiloka, H.
  • Journal Title: Plant Cell Volume: (印刷中,掲載確定)
  • Peer Reviewed
• [Journal Article] A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance. (2010)
  • Author(s): Asai, S., Mase, K., Yoshioka, H.
  • Journal Title: Plant J. 62 Pages: 911-924
  • Peer Reviewed
• [Journal Article] Role of nitric oxide and reactive oxide species in disease resistance to necrotrophic pathogens. (2010)
  • Author(s): Asai, S., Muse, K., Yoshioka, H.
  • Journal Title: Plant Signal.Behav. 5 Pages: 872-874
  • Peer Reviewed
• [Journal Article] A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance (2010)
  • Author(s): Asai, S., Mase, K., Yoshioka, H.
  • Journal Title: Plant J. (印刷中)
  • Peer Reviewed
• [Journal Article] Role of nitric oxide and reactive oxide species in disease resistance to necrotrophic pathogens (2010)
  • Author(s): Asai, S., Mase, K., Yoshioka, H.
  • Journal Title: Plant Signal.Behav. (印刷中)
  • Peer Reviewed
• [Journal Article] Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen Botrvtis cinerea in Nicotiana benthamiana. (2009)
  • Author(s): Asai, S., Yoshioka, H.
  • Journal Title: Mol.Plant-Microbe Interact. 22 Pages: 619-629
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity. (2009)
  • Author(s): Yoshioka, H., Asai, S., Yoshioka, M., Kobayashi, M.
  • Journal Title: Mol.Cells 28 Pages: 321-329
  • Peer Reviewed
• [Journal Article] Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen Botrytis cinerea in Nicotiana benthamiana (2009)
  • Author(s): Asai, S., Yoshioka, H.
  • Journal Title: Mol.Plant-Microbe Interact 22 Pages: 619-629
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity (2009)
  • Author(s): Yoshioka, H., Asai, S., Yoshioka, M., Kobayashi, M.
  • Journal Title: Mol.Cells 28 Pages: 321-329
  • Peer Reviewed
• [Journal Article] Nitric oxide as a partner of reactive oxygen species participates in disease resistance to necrotrophic pathogen Botrytis cinerea in Nicotiana benthamiana. (2009)
  • Author(s): Asai, S., et al.
  • Journal Title: Mol. Plant-Microbe Interact. 22 Pages: 619-629
  • Peer Reviewed
• [Journal Article] MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. (2008)
  • Author(s): Asai, S., Ohta, K., Yoshioka, H.
  • Journal Title: Plant Cell 20 Pages: 1390-1406
  • Peer Reviewed
• [Journal Article] The role of radical burst via MAPK signaling in plant immunity. (2008)
  • Author(s): Asai, S., Yoshioka, H.
  • Journal Title: Plant Signal.Behav. 3 Pages: 920-922
  • Peer Reviewed
• [Journal Article] MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana (2008)
  • Author(s): Asai, S., Ohta, K., Yoshioka, H.
  • Journal Title: Plant Cell 20 Pages: 1390-1406
  • Peer Reviewed
• [Journal Article] MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. (2008)
  • Author(s): Asai, S., et al.
  • Journal Title: Plant Cell 20 Pages: 1390-1406
  • Peer Reviewed
• [Journal Article] The role of radical burst via MAPK signaling in plant immunity (2008)
  • Author(s): Asai, S., Yoshioka H.
  • Journal Title: Plant Signal. Behavior 3 Pages: 920-922
  • Peer Reviewed
• [Journal Article] Involvement of NbNOAl in NO production and defense responses in INFl-treated Nicotiana benthamiana. (2008)
  • Author(s): Kato, H.
  • Journal Title: Journal of General Plant Pathology 74 Pages: 15-23
  • Peer Reviewed
• [Journal Article] Induction of a small heat shock protein and its functional roles in Nicotiana plants in the defense response against Ralstonia solanacearum (2007)
  • Author(s): Maimbo, M.
  • Journal Title: Plant Physiology 145 Pages: 1588-1599
  • Peer Reviewed
• [Journal Article] Regulation of rice NADPH oxidase by binding of Rac GTPase to its N-terminal extension. (2007)
  • Author(s): Wong, H. L.
  • Journal Title: Plant Cell 19 Pages: 4022-4034
  • Peer Reviewed
• [Journal Article] A key enzyme for flavin synthesis is required for nitric oxide and reactive oxygen species production in disease resistance
  • Author(s): Asai, S., Mase, K., Yoshioka, H.
  • Journal Title: Plant J.
  • Peer Reviewed
• [Presentation] MAPK and CDPK control NO and ROS bursts in plant immune response. (2011)
  • Author(s): Hirofumi Yoshioka
  • Organizer: The XV International Congress on Molecular Plant-Microbe Interactions(発表確定)
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2011-08-04
• [Presentation] WRKY8のMAPKによるリン酸化にはD domain依存的な相互作用が必要である. (2011)
  • Author(s): 石濱伸明・山田麗子・吉岡博文
  • Organizer: 第52回日杢植物生理学会年会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] Fumonisin B1によるプログラム細胞死にはエチレンシグナル経路が関与する. (2011)
  • Author(s): 間瀬圭介・石濱伸明・森仁志・児玉基一朗・吉岡博
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] 植物免疫におけるラジカルバーストに関わるタンパク質の機能解析 (2011)
  • Author(s): 野村裕也・藤原左幸・深尾陽一朗・椎名隆・吉岡博文
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] MAPKカスケードに依存した一酸化窒素合成に関与する因子の探索. (2011)
  • Author(s): 橋本良平・野村裕也・浅井秀太・久保康之・吉岡博文
  • Organizer: 23年度日本植物病理学会大会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] グルタチオンペルオキシダーゼNuGPXIはAAL毒素による細胞死に関与する. (2011)
  • Author(s): 藤井孝行・間瀬圭介・浅井秀太・吉岡美樹・野村裕也・児玉基一朗・吉岡博文
  • Organizer: 23年度日本植物病理学会大会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] エチレンシグナル経路はFumonisin B1によるプログラム細胞死に関与する. (2011)
  • Author(s): 間瀬圭介・石濱伸明・水野貴仁・森仁志・児玉基一朗・吉岡博文
  • Organizer: 23年度日本植物病理学会大会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] D domain依存的な直接の相互作用がWRKY8のMAPKによるリン酸化には必要である. (2011)
  • Author(s): 石濱伸明・山田麗子・吉岡博文
  • Organizer: 23年度日本植物病理学会大会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] 植物免疫におけるラジカルバースト制御タンパク質のプロテオーム解析. (2011)
  • Author(s): 野村裕也・藤原正幸・深尾陽一朗・椎名隆・吉岡博文
  • Organizer: 23年度日本植物病理学会大会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-11
• [Presentation] 物免疫応答における脚キナーゼの役割 (2011)
  • Author(s): 吉岡博文
  • Organizer: 第3回植物ストレス科学研究シンポジウム並びに第27回資源植物科学シンポジウム
  • Place of Presentation: 倉敷市芸分館ホール(岡山県)
  • Year and Date: 2011-03-08
• [Presentation] WRKY8のMAPKによるリン酸化にはD domain依存的な相互作用が必要である (2011)
  • Author(s): 石濱伸呪・山田麗子・吉岡博文
  • Organizer: 要旨集
• [Presentation] AAL毒素による細胞死におけるSIPKとWIPKの役割 (2010)
  • Author(s): 間瀬圭介・水野貴仁・森仁志・児玉基一朗・吉岡博文
  • Organizer: 平成22年度日本植物生理学会関西部会
  • Place of Presentation: AOSSA(福井県)
  • Year and Date: 2010-09-30
• [Presentation] Regulatory mechanisms of NO and ROS generation and role in plantimmunity. (2010)
  • Author(s): Yoshioka, H., Mase, K., Yoshioka, M., Kobayashi, M., Asai, S.
  • Organizer: 6th International Conference on the Biology, Chemistly, and Therapeutic Application of Nitric Oxide.
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2010-06-16
• [Presentation] Regulation of NADPH oxidase-mediated ROS generation and role in plant immunity (2010)
  • Author(s): Yoshioka H.
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
  • Year and Date: 2010-03-20
• [Presentation] Regulatory mechanisms of NO and ROS generation and role in plant immunity. (2010)
  • Author(s): Yoshioka H., Mase, K., Yoshioka M., Kobayashi M. Asai,S.
  • Organizer: 6th International Conference on the Biology Chemistly, and Therapeutic Application of Nitric Oxide.
  • Place of Presentation: 京都国際会議場,京都府
• [Presentation] MAPKによるWRKY型転写因子のリン酸化が植物免疫応答の誘導に関与する. (2010)
  • Author(s): 石濱伸明・山田麗子・吉岡博文
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 国立京都国際会館,京都市
• [Presentation] MAPKによりリン酸化されるWRKY型転写因子が植物免疫応答の誘導に関与する. (2010)
  • Author(s): 石濱伸明・吉岡博文
  • Organizer: ボスター発表第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学,熊本市
• [Presentation] Regulation of NADPH oxidase-mediated ROS generation and role in plant immunity. (2010)
  • Author(s): 吉岡博文
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学,熊本市
• [Presentation] Riboflavin synthesis participates in regulation of nitric oxide and reactive oxygen species production. (2010)
  • Author(s): Asai, S., Mase, K., Yoshioka H.
  • Organizer: Genome-Enabled Integration of Research in Plant Pathogen Systems.Poster, 10th US-Japan seminar
  • Place of Presentation: Oregon, USA
• [Presentation] Nitric oxide and reactive oxygen species in plant defense responses. Genome-Enabled Integration of Research in Plant Pathogen Systems. (2010)
  • Author(s): Yoshioka H., Asai, S., Yoshioka M., Kobayashi M., Doke, N.
  • Organizer: 10th US-Japan seminar
  • Place of Presentation: Oregon, USA
• [Presentation] MAPKの基質であるWRKY8は植物の防御応答に関与する. (2009)
  • Author(s): 石濱伸明, 山田麗子, 吉岡博文
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋市
  • Year and Date: 2009-03-22
• [Presentation] Molecular mechanisms of generation for nitric oxide and reactive oxygen species, and role of the radical burst in plant immunity. (2009)
  • Author(s): 吉岡博文
  • Organizer: The 21st Annual Meeting of the Korean Society for Molecular and Cellular Biology "RNA Biology and Disease"
  • Place of Presentation: Seoul, Korea
• [Presentation] StCDPK5のvaluabieドメインおよびkinaseドメインがStRBOHBの活性化に重要である (2009)
  • Author(s): 浅井秀太・市川達士・小林光智衣・上吉原裕介・森仁志・吉岡博文
  • Organizer: 平成21年度日本植物病理学会関西部会
  • Place of Presentation: 神戸市
• [Presentation] MAPKの基質であるNbWRKY8はHMGR2の発現を正に制御する (2009)
  • Author(s): 石濱伸明・山田麗子・吉岡博文
  • Organizer: 平成21年度日本植物病理学会関西部会
  • Place of Presentation: 神戸市
• [Presentation] 活性酸素および活性窒素の生成機構および病害抵抗性における役割について (2009)
  • Author(s): 浅井秀太・小林光智衣・吉岡美樹・吉岡博文
  • Organizer: 植物微生物研究会第19回研究交流会,あがたの森文化会館
  • Place of Presentation: 松本市
• [Presentation] Riboflavin synthesis participates in plant immune responses in Nicotiana benthamiana. (2009)
  • Author(s): Asai, S., Mase K., Yoshioka H.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions.
  • Place of Presentation: Quebec, Canada
• [Presentation] MAPKs phosphorylate WRKYB and induce defense-related genes in Nicotiana benthamiana. (2009)
  • Author(s): 石濱伸明, 山田麗子, 吉岡博文
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions.
  • Place of Presentation: Quebec, Canada
• [Presentation] Transgenic potato plants expressing constitutively active StCDPK5 show late blight resistance, but early blight susceptibility. (2009)
  • Author(s): Yoshioka M., Kobayashi M., Asai, S., Yoshioka H.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions.
  • Place of Presentation: Quebec, Canada
• [Presentation] MAPKの基質であるWRKY8は植物の防御応答に関与する (2009)
  • Author(s): 石濱伸明・山田麗子・吉岡博文
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学,名古屋市
• [Presentation] MAPKs phosphorylate WRKY8 and induce defense-related genes in Nicotiana benthamiana (2009)
  • Author(s): Ishihama, N., Yamada, R., Yoshioka, H.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Quebec, Canada
• [Presentation] Involvement of maitogen-activated protein kinase substrate PPS8 in plant immunity. (2007)
  • Author(s): Ishihama, N.
  • Organizer: XIII International Congress on Molecular Plant-Microbe Interactions.
  • Place of Presentation: イタリア
• [Book] In Genome-Enabled Analysis of Plant-Pathogen Interactions.((Wolpert, T., Shiraishi, T., Allen, C., Akimitsu, K.and Glazebrook, J.eds)(Nitric oxide and reactive oxygen species in plant defense responses.) (2011)
  • Author(s): Yoshioka, H., Asai, S., Yoshioka, M., Kobayashi, M., Doke, N.
  • Publisher: APS Press, MN
• [Book] Nitric oxide and reactive oxygen species in plant defense responses. In Genome-Enabled Analysis of Plant-Pathogen Interactions. (2011)
  • Author(s): Yoshioka, H., Asai, S., Yoshioka, M., Kobayashi, M., Doke, N.
  • Total Pages: 272
  • Publisher: APS Press
• [Book] Proceedings of the 14th International Congress on Molecular Plant-Microbe Interactions.(Antoun, H., Avis, T., Brisson, L., Prevost, D.and Trepanier, M.eds)(Riboflavin synthesis participates in plant immune responses in Nicotiana benthamiana. In Biology of Plant-Microbe Interactions Vol.7.Chapter 28) (2010)
  • Author(s): Asai, S.Mase, K., Yoshioka, H.
  • Publisher: IS-MPMI Press, MN
• [Book] Nitric oxide and reactive oxygen species in plant defense responses. In Genome-Enabled Integration of Research in Plant Pathogen Systems (2010)
  • Author(s): Yoshioka, H., Asai, S., Yoshioka, M., Kobayashi, M., Doke, N.
  • Publisher: APS Press(印刷中)
• [Book] In Molecular Plant-Microbe Interactions.(Bouarab, K., Brisson, N.and Daayf, F.Eds)(Molecular mechanisms of the radical burst in plant immunity. In Molecular Plant-Microbe Interactions.) (2009)
  • Author(s): Yoshioka, H., Asai, S., Miyagawa, N., Ichikawa, T., Yoshioka, M., Kobayashi, M.
  • Publisher: CAB International, Oxfordshire, UK
• [Book] Molecular Plant-Microbe Interactions.(Bouarab, K., Brisson, N. and Daayf, F. eds, Molecular mechanisms of the radical burst in plant immunity.) (2009)
  • Author(s): Yoshioka, H., Asai, S., Miyagawa, N., Ichikawa, T., Yoshioka, M., Kobayashi, M.
  • Publisher: CAB International
• [Book] Molecular mechanisms of the radical burst in plant immunity. In Molecular Plant-Microbe Interactions. (2009)
  • Author(s): Yoshioka H., et al.
  • Publisher: CABI press(印刷中)
• [Book] 植物免疫におけるラジカルバーストの分子機構.植物-病原微生物の相互作用のダイナミズム. (2007)
  • Author(s): 吉岡博文
  • Total Pages: 11
  • Publisher: 日本植物病理学会
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