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Improvement of nitrogen-use-efficiency based on molecular mechanisms of ammonium assimilation and nitrogen recycling system in rice

Research Project

Project/Area Number 19208007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTohoku University

Principal Investigator

YAMAYA Tomoyuki  Tohoku University, 大学院・農学研究科, 教授 (30144778)

Co-Investigator(Kenkyū-buntansha) SATO Tadashi  東北大学, 大学院・生命科学研究科, 准教授 (40134043)
Co-Investigator(Renkei-kenkyūsha) SATO Tadashi  東北大学, 大学院・生命科学研究科, 准教授 (40134043)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥39,520,000 (Direct Cost: ¥30,400,000、Indirect Cost: ¥9,120,000)
Fiscal Year 2009: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2007: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Keywordsイネ / アンモニア同化 / 窒素リサイクル機構 / 窒素利用効率 / 遺伝子破壊 / サイトゾル型グルタミン合成酵素 / NADH-グルタミン酸合成酵素 / 遺伝子破壊変異体 / QTL解析 / 遺伝子機能解明 / トランスクリプトーム
Research Abstract

Two new enzymes, GS1;3 and NADH-GOGAT, related to the primary assimilation of ammonium ions in rice discovered after the complete sequencing of genome were focused to understand their functions. Reverse genetics approaches successfully showed that GS1;3 is important during the seed germination, while NADH-GOGAT2 is functioning during nitrogen remobilization during leaf senescence. A new gene related to the nitrogen use efficiency was identified with the forward genetics and it contributed the increase in root-elongation as well as nitrogen uptake in rice.

Report

(5 results)
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (64 results)

All 2010 2009 2008 2007 Other

All Journal Article (25 results) (of which Peer Reviewed: 24 results) Presentation (21 results) Book (9 results) Remarks (9 results)

  • [Journal Article] Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH_4^+ concentration in hydroponic conditions.2010

    • Author(s)
      Obara M., Tamura W., Ebitani T., Yano M., Sato T., Yamaya, T.
    • Journal Title

      Theoretical Applied Genetics 121

      Pages: 535-547

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthase1 in rice plants.2010

    • Author(s)
      Tamura W., Hidaka Y., Tabuchi M., Kojima S., Hayakawa T., Sato T., Obara M., Kojima M., Sakakibara H., Yamaya T.
    • Journal Title

      Amino Acids 39

      Pages: 1003-1012

    • NAID

      120006376372

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH_4^+ concentration in hydroponic conditions2010

    • Author(s)
      Obara, M., Tamura, W., Ebitani, T., Yano, M., Sato, T., Yamaya, T.
    • Journal Title

      Theoretical Applied Genetics (in press)

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthasel in rice plants2010

    • Author(s)
      Tamura, W., Hidaka, Y., Tabuchi, M., Kojima, S., Hayakawa, T., Sato, T., Obara, M., Kojima, M., Sakakibara, H., Yamaya, T.
    • Journal Title

      Amino Acids (in press)

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthasel in rice plants2010

    • Author(s)
      Tamura, et al.
    • Journal Title

      Amino Acids (未定, In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      山谷知行
    • Journal Title

      植物栄養学 第2版(文永堂出版)

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase 2 gene.2009

    • Author(s)
      Takahashi H., Takahara K., Hashida S., Hirabayashi T., Fujimori T., Kawai-Yamada M., Yamaya T., Yanagisawa S., Uchimiya H.
    • Journal Title

      Plant Physiol 151

      Pages: 100-113

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase 2 gene2009

    • Author(s)
      Takahashi, H., Takahara, K., Hashida, S., Hirabayashi, T., Fujimori, T., Kawai-Yamada, M., Yamaya, T., Yanagisawa, S., Uchimiya, H.
    • Journal Title

      Plant Physiol 151

      Pages: 100-113

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Cytosolic glutamine synthetase is present in phloem sap from rice(Oryza sativa L.)2009

    • Author(s)
      Tanaka, et al.
    • Journal Title

      Soil Science and Plant Nutrition 55

      Pages: 102-106

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plerotropic modulation of carbon and nitrogen metabokism in Arabidopsis plants overexpressing NAD kinase 2 gene2009

    • Author(s)
      Takahashi, et al.
    • Journal Title

      Plant Physiology 151

      Pages: 100-103

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytosolic glutamine synthetase is present in phloem sap from rice(Oryza sativa L.)2009

    • Author(s)
      Tanaka, et al.
    • Journal Title

      Soil Science Plant Nutrition 55

      Pages: 102-106

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.)2008

    • Author(s)
      Kudo T., Kawai A., Yamaya T., Hayakawa T.
    • Journal Title

      Physiol.Plant. 133

      Pages: 167-179

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis.2008

    • Author(s)
      Sugawara H., Ueda N., Kojima M., Makita N., Yamaya T., Sakakibara H.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 105

      Pages: 2734-2739

    • NAID

      130006995125

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.)2008

    • Author(s)
      Kudo, T., Kawai, A., Yamaya, T., Hayakawa, T.
    • Journal Title

      Physiol. Plant. 133

      Pages: 167-179

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis2008

    • Author(s)
      Sugawara, H., Ueda, N., Kojima, M., Makita, N., Yamaya, T., Sakakibara, H.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 105

      Pages: 2734-2739

    • NAID

      130006995125

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis2008

    • Author(s)
      Sugawara, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 105

      Pages: 2734-2739

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice(Oryza sativa L.)2008

    • Author(s)
      Kudo, et al.
    • Journal Title

      Physiologia Plantaram 133

      Pages: 169-179

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis2008

    • Author(s)
      Sugawara, et. al.
    • Journal Title

      Proc. Natl. Acad. Sci. USA 105

      Pages: 2734-2739

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice(Oryza sativa L.)2008

    • Author(s)
      Kudo, et. al.
    • Journal Title

      Physiologia Plantaram (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene.2007

    • Author(s)
      Suzuki Y., Ohkubo M., Hatakeyama H., Ohashi K., Yoshizawa R., Kojima S., Hayakawa T., Yamaya T., Mae T., Makino A.
    • Journal Title

      Plant Cell Physiol. 48

      Pages: 626-637

    • NAID

      10027001603

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice (Oryza sativa L.).2007

    • Author(s)
      Tabuchi M., Abiko T., Yamaya T.
    • Journal Title

      J.Exp.Bot. 58

      Pages: 2319-2327

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased Rubisco content in transgenic rice transfbrmed with "sense" rbcS gene2007

    • Author(s)
      Suzuki, Y., Ohkubo, M., Hatakeyama, H., Ohashi, K., Yoshizawa, R., Kojima, S., Hayakawa, T., Yamaya, T., Mae, T., Makino, A.
    • Journal Title

      Plant Cell Physiol 48

      Pages: 626-637

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice (Oryza sativa L)2007

    • Author(s)
      Tabuchi, M., Abiko, T., Yamaya, T.
    • Journal Title

      J.Exp.Bot. 58

      Pages: 2319-2327

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice(Oryza sativa L.)2007

    • Author(s)
      Tabuchi, et. al.
    • Journal Title

      Journal of Experimental Botany 58

      Pages: 2319-2327

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene2007

    • Author(s)
      Suzuki, et. al.
    • Journal Title

      Plant Cell Physiology 48

      Pages: 626-637

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] シロイヌナズナ高親和型尿素輸送担体の相互作用因子の探索2010

    • Author(s)
      吉田圭吾, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネにおけるNADH依存性グルタミン酸合成酵素(NADH-GOGAT)1及び2遺伝子の破壊変異体の獲得とその表現型2010

    • Author(s)
      田村亘, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] シロイヌナズナ環境型のアンモニウム栄養に対する応答2010

    • Author(s)
      大岩優貴, ら
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Function of glutamine synthetase and glutamate synthase in rice plants2009

    • Author(s)
      Yamaya, T.
    • Organizer
      11^<th> Inthernational Congress on Amino Acids, Peptide and Proteins
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2009-08-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネの窒素飢餓ストレス2009

    • Author(s)
      山谷知行
    • Organizer
      日本土壌肥料学会2009年度京都大会シンポジウム「植物のミネラルストレス応答」
    • Place of Presentation
      京都(招待講演)
    • Related Report
      2009 Final Research Report
  • [Presentation] Function of glutamine synthetase and glutamate synthase in rice plants.2009

    • Author(s)
      山谷知行
    • Organizer
      11^<th> International Congress on Amino Acids, Peptides and Proteins
    • Place of Presentation
      Vienna, Austria(招待講演)
    • Related Report
      2009 Final Research Report
  • [Presentation] イネの窒素飢餓ストレス2009

    • Author(s)
      山谷知行
    • Organizer
      日本土壌肥料学会2009年度京都大会シンポジウム「植物のミネラルストレス応答」
    • Place of Presentation
      京都 (招待講演)
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Function of glutamine synthetase and glutamate synthase in rice plants. 11^<th> International Congress on Amino Acids2009

    • Author(s)
      Yamaya, T.
    • Organizer
      Peptides and Proteins
    • Place of Presentation
      Vienna, Austria (招待講演)
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] イネNADH依存性グルタミン酸合成酵素1(NADH-GOGAT1)破壊変異体の獲得と表現型の解析2009

    • Author(s)
      田村亘, ら
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] サイトゾル型グルタミン合成酵素欠損変異イネの幼植物における解析2009

    • Author(s)
      田渕真由美, ら
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] 液胞型アクアアポリンの細胞膜アンモニウム輸送への寄与2009

    • Author(s)
      小島創一, ら
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネ根におけるGlnセンサー候補OsACTPK遺伝子族の発現特性とOsACTPK4遺伝子破壊イネの解析2009

    • Author(s)
      澤優己, ら
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] イネ根系の発達を促進するQTL遺伝子の単離2009

    • Author(s)
      小原実広, ら
    • Organizer
      日本土壌肥料学会2009年度京都大会
    • Place of Presentation
      京都大学
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic and physiological approach of nitrogen utilization toward improving environmental adaptation of rice.2008

    • Author(s)
      Obara M., Yamaya T.
    • Organizer
      Workshop on Development of Environmentally-friendly Water-saving Technologies for Rice
    • Place of Presentation
      Tsukuba、Japan(招待講演)
    • Year and Date
      2008-07-18
    • Related Report
      2009 Final Research Report
  • [Presentation] Genetic and physiological approach of nitrogen utilization toward improving environmental adaptation of rice. Workshop on Development of Environmentally-friendly2008

    • Author(s)
      Obara, M., Yamaya, T.
    • Organizer
      Water-saving Technologies for Rice
    • Place of Presentation
      Tsukuba, Japan (招待講演)
    • Year and Date
      2008-07-18
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Molecular mechanisms for plant biomass production-rice as a model plant.2008

    • Author(s)
      山谷知行
    • Organizer
      UCR Tech Horizon 2008 Conference
    • Place of Presentation
      UC Riverside, CA, USA(招待講演)
    • Related Report
      2009 Final Research Report
  • [Presentation] Molecular mechanisms for plant biomass production-rice as a model plant2008

    • Author(s)
      Yamaya, T.
    • Organizer
      UCR Tech Horizon 2008 Conference
    • Place of Presentation
      UC Riverside, CA, USA (招待講演)
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Molecular mechanisms for plant biomass production -rice as a model plant2008

    • Author(s)
      Yamaya, T.
    • Organizer
      UCR Tech Horizon 2008 Conference
    • Place of Presentation
      UC Riverside, CA, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice.2007

    • Author(s)
      山谷知行
    • Organizer
      Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Lancaster University, UK(招待講演)
    • Related Report
      2009 Final Research Report
  • [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice2007

    • Author(s)
      Yamaya, T.
    • Organizer
      Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Lancaster University, UK (招待講演)
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice2007

    • Author(s)
      Yamaya, T.
    • Organizer
      Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Lancaster University, UK
    • Related Report
      2007 Annual Research Report
  • [Book] Nitrogen Assimilation in Plants(India, Ammonia absorption and assimilation in plants)2010

    • Author(s)
      Kojima K., von Wiren N., Yamaya T.
    • Publisher
      Research Signpost 社
    • Related Report
      2009 Final Research Report
  • [Book] 植物栄養学 第2版(間藤徹、馬建鋒、藤原徹編)(アンモニウムイオンの吸収、アンモニアトランの同化)2010

    • Author(s)
      山谷知行
    • Publisher
      文永堂出版
    • Related Report
      2009 Final Research Report
  • [Book] India, Ammonia absorption and assimilation in plants, In : "Nitrogen Assinlilation in Plants"2010

    • Author(s)
      Kojima, K., von Wiren, N., Yamaya, T.
    • Publisher
      Research Signpost 社(印刷中)
    • Related Report
      2009 Self-evaluation Report
  • [Book] 細胞内アンモニア代謝、アンモニアトランスポーター、「植物栄養学 第2版」間藤徹, 馬建鋒, 藤原徹, 編2010

    • Author(s)
      山谷知行
    • Publisher
      文永堂出版(印刷中)
    • Related Report
      2009 Self-evaluation Report
  • [Book] Nitrogen Assimilation in Plants2010

    • Author(s)
      Kojima, et al.
    • Publisher
      Research Signpost, India(印刷中)
    • Related Report
      2009 Annual Research Report
  • [Book] 植物の百科事典(b窒素代謝)2009

    • Author(s)
      山谷知行
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] 植物の百科事典(1.2 物質代謝)2009

    • Author(s)
      山谷知行
    • Publisher
      朝倉書店
    • Related Report
      2009 Final Research Report
  • [Book] b窒素代謝、「植物の百科事典」2009

    • Author(s)
      山谷知行
    • Total Pages
      16
    • Publisher
      朝倉書店
    • Related Report
      2009 Self-evaluation Report
  • [Book] 1. 2物質代謝、「植物の百科事典」2009

    • Author(s)
      山谷知行
    • Publisher
      朝倉書店
    • Related Report
      2009 Self-evaluation Report
  • [Remarks] 報道関連情報

    • Related Report
      2009 Final Research Report
  • [Remarks] 山谷知行、東北大学の新世紀-世界をリードするイネの研究、2009年6月29日、東日本放送

    • Related Report
      2009 Final Research Report
  • [Remarks] アウトリーチ活動

    • Related Report
      2009 Final Research Report
  • [Remarks] 山谷知行、植物の基盤研究から食料自給率を考える。文部科学省政策会議先端科学技術調査会「植物科学研究が切り拓く未来-グリーンイノベーションに向けて」、衆議院第1議員会館民主党A会議室、2010年3月4日

    • Related Report
      2009 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2009 Final Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.agri.tohoku.ac.jp/cellbio/index-j.htm

    • Related Report
      2007 Annual Research Report

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Published: 2007-04-01   Modified: 2016-04-21  

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