Improvement of nitrogen-use-efficiency based on molecular mechanisms of ammonium assimilation and nitrogen recycling system in rice

• Project/Area Number: 19208007
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Tohoku University
• Principal Investigator: YAMAYA Tomoyuki Tohoku University, 大学院・農学研究科, 教授 (30144778)
• Co-Investigator(Kenkyū-buntansha): SATO Tadashi 東北大学, 大学院・生命科学研究科, 准教授 (40134043)
• Co-Investigator(Renkei-kenkyūsha): SATO Tadashi 東北大学, 大学院・生命科学研究科, 准教授 (40134043)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥39,520,000 (Direct Cost: ¥30,400,000、Indirect Cost: ¥9,120,000); Fiscal Year 2009: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2008: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2007: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
• Keywords: イネ / アンモニア同化 / 窒素リサイクル機構 / 窒素利用効率 / 遺伝子破壊 / サイトゾル型グルタミン合成酵素 / NADH-グルタミン酸合成酵素 / 遺伝子破壊変異体 / QTL解析 / 遺伝子機能解明 / トランスクリプトーム

Research Abstract

Two new enzymes, GS1;3 and NADH-GOGAT, related to the primary assimilation of ammonium ions in rice discovered after the complete sequencing of genome were focused to understand their functions. Reverse genetics approaches successfully showed that GS1;3 is important during the seed germination, while NADH-GOGAT2 is functioning during nitrogen remobilization during leaf senescence. A new gene related to the nitrogen use efficiency was identified with the forward genetics and it contributed the increase in root-elongation as well as nitrogen uptake in rice.

Research Products

• [Journal Article] Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH_4^+ concentration in hydroponic conditions. (2010)
  • Author(s): Obara M., Tamura W., Ebitani T., Yano M., Sato T., Yamaya, T.
  • Journal Title: Theoretical Applied Genetics 121 Pages: 535-547
  • Peer Reviewed
• [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthase1 in rice plants. (2010)
  • Author(s): Tamura W., Hidaka Y., Tabuchi M., Kojima S., Hayakawa T., Sato T., Obara M., Kojima M., Sakakibara H., Yamaya T.
  • Journal Title: Amino Acids 39 Pages: 1003-1012
  • NAID: 120006376372
  • Peer Reviewed
• [Journal Article] Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH_4^+ concentration in hydroponic conditions (2010)
  • Author(s): Obara, M., Tamura, W., Ebitani, T., Yano, M., Sato, T., Yamaya, T.
  • Journal Title: Theoretical Applied Genetics (in press)
  • Peer Reviewed
• [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthasel in rice plants (2010)
  • Author(s): Tamura, W., Hidaka, Y., Tabuchi, M., Kojima, S., Hayakawa, T., Sato, T., Obara, M., Kojima, M., Sakakibara, H., Yamaya, T.
  • Journal Title: Amino Acids (in press)
  • Peer Reviewed
• [Journal Article] Reverse genetics approach to characterize a function of NADH-glutamate synthasel in rice plants (2010)
  • Author(s): Tamura, et al.
  • Journal Title: Amino Acids (未定, In press)
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 山谷知行
  • Journal Title: 植物栄養学 第2版(文永堂出版)
• [Journal Article] Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase 2 gene. (2009)
  • Author(s): Takahashi H., Takahara K., Hashida S., Hirabayashi T., Fujimori T., Kawai-Yamada M., Yamaya T., Yanagisawa S., Uchimiya H.
  • Journal Title: Plant Physiol 151 Pages: 100-113
  • Peer Reviewed
• [Journal Article] Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase 2 gene (2009)
  • Author(s): Takahashi, H., Takahara, K., Hashida, S., Hirabayashi, T., Fujimori, T., Kawai-Yamada, M., Yamaya, T., Yanagisawa, S., Uchimiya, H.
  • Journal Title: Plant Physiol 151 Pages: 100-113
  • Peer Reviewed
• [Journal Article] Cytosolic glutamine synthetase is present in phloem sap from rice(Oryza sativa L.) (2009)
  • Author(s): Tanaka, et al.
  • Journal Title: Soil Science and Plant Nutrition 55 Pages: 102-106
  • Peer Reviewed
• [Journal Article] Plerotropic modulation of carbon and nitrogen metabokism in Arabidopsis plants overexpressing NAD kinase 2 gene (2009)
  • Author(s): Takahashi, et al.
  • Journal Title: Plant Physiology 151 Pages: 100-103
  • Peer Reviewed
• [Journal Article] Cytosolic glutamine synthetase is present in phloem sap from rice(Oryza sativa L.) (2009)
  • Author(s): Tanaka, et al.
  • Journal Title: Soil Science Plant Nutrition 55 Pages: 102-106
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.) (2008)
  • Author(s): Kudo T., Kawai A., Yamaya T., Hayakawa T.
  • Journal Title: Physiol.Plant. 133 Pages: 167-179
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis. (2008)
  • Author(s): Sugawara H., Ueda N., Kojima M., Makita N., Yamaya T., Sakakibara H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 105 Pages: 2734-2739
  • NAID: 130006995125
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice (Oryza sativa L.) (2008)
  • Author(s): Kudo, T., Kawai, A., Yamaya, T., Hayakawa, T.
  • Journal Title: Physiol. Plant. 133 Pages: 167-179
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis (2008)
  • Author(s): Sugawara, H., Ueda, N., Kojima, M., Makita, N., Yamaya, T., Sakakibara, H.
  • Journal Title: Proc.Natl.Acad.Sci.USA 105 Pages: 2734-2739
  • NAID: 130006995125
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis (2008)
  • Author(s): Sugawara, et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA 105 Pages: 2734-2739
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice(Oryza sativa L.) (2008)
  • Author(s): Kudo, et al.
  • Journal Title: Physiologia Plantaram 133 Pages: 169-179
  • Peer Reviewed
• [Journal Article] Structural insight into the reaction mechanism and evolution of cytokinin biosynthesis (2008)
  • Author(s): Sugawara, et. al.
  • Journal Title: Proc. Natl. Acad. Sci. USA 105 Pages: 2734-2739
  • Peer Reviewed
• [Journal Article] Cellular distribution of ACT domain repeat protein 9, a nuclear localizing protein, in rice(Oryza sativa L.) (2008)
  • Author(s): Kudo, et. al.
  • Journal Title: Physiologia Plantaram (In press)
  • Peer Reviewed
• [Journal Article] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene. (2007)
  • Author(s): Suzuki Y., Ohkubo M., Hatakeyama H., Ohashi K., Yoshizawa R., Kojima S., Hayakawa T., Yamaya T., Mae T., Makino A.
  • Journal Title: Plant Cell Physiol. 48 Pages: 626-637
  • NAID: 10027001603
  • Peer Reviewed
• [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice (Oryza sativa L.). (2007)
  • Author(s): Tabuchi M., Abiko T., Yamaya T.
  • Journal Title: J.Exp.Bot. 58 Pages: 2319-2327
  • Peer Reviewed
• [Journal Article] Increased Rubisco content in transgenic rice transfbrmed with "sense" rbcS gene (2007)
  • Author(s): Suzuki, Y., Ohkubo, M., Hatakeyama, H., Ohashi, K., Yoshizawa, R., Kojima, S., Hayakawa, T., Yamaya, T., Mae, T., Makino, A.
  • Journal Title: Plant Cell Physiol 48 Pages: 626-637
  • Peer Reviewed
• [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice (Oryza sativa L) (2007)
  • Author(s): Tabuchi, M., Abiko, T., Yamaya, T.
  • Journal Title: J.Exp.Bot. 58 Pages: 2319-2327
  • Peer Reviewed
• [Journal Article] Assimilation of ammonium-ions and re-utilization of nitrogen in rice(Oryza sativa L.) (2007)
  • Author(s): Tabuchi, et. al.
  • Journal Title: Journal of Experimental Botany 58 Pages: 2319-2327
  • Peer Reviewed
• [Journal Article] Increased Rubisco content in transgenic rice transformed with "sense" rbcS gene (2007)
  • Author(s): Suzuki, et. al.
  • Journal Title: Plant Cell Physiology 48 Pages: 626-637
  • Peer Reviewed
• [Presentation] シロイヌナズナ高親和型尿素輸送担体の相互作用因子の探索 (2010)
  • Author(s): 吉田圭吾, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] イネにおけるNADH依存性グルタミン酸合成酵素(NADH-GOGAT)1及び2遺伝子の破壊変異体の獲得とその表現型 (2010)
  • Author(s): 田村亘, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] シロイヌナズナ環境型のアンモニウム栄養に対する応答 (2010)
  • Author(s): 大岩優貴, ら
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] Function of glutamine synthetase and glutamate synthase in rice plants (2009)
  • Author(s): Yamaya, T.
  • Organizer: 11^<th> Inthernational Congress on Amino Acids, Peptide and Proteins
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-08-03
• [Presentation] イネの窒素飢餓ストレス (2009)
  • Author(s): 山谷知行
  • Organizer: 日本土壌肥料学会2009年度京都大会シンポジウム「植物のミネラルストレス応答」
  • Place of Presentation: 京都(招待講演)
• [Presentation] Function of glutamine synthetase and glutamate synthase in rice plants. (2009)
  • Author(s): 山谷知行
  • Organizer: 11^<th> International Congress on Amino Acids, Peptides and Proteins
  • Place of Presentation: Vienna, Austria(招待講演)
• [Presentation] イネの窒素飢餓ストレス (2009)
  • Author(s): 山谷知行
  • Organizer: 日本土壌肥料学会2009年度京都大会シンポジウム「植物のミネラルストレス応答」
  • Place of Presentation: 京都 (招待講演)
• [Presentation] Function of glutamine synthetase and glutamate synthase in rice plants. 11^<th> International Congress on Amino Acids (2009)
  • Author(s): Yamaya, T.
  • Organizer: Peptides and Proteins
  • Place of Presentation: Vienna, Austria (招待講演)
• [Presentation] イネNADH依存性グルタミン酸合成酵素1(NADH-GOGAT1)破壊変異体の獲得と表現型の解析 (2009)
  • Author(s): 田村亘, ら
  • Organizer: 日本土壌肥料学会2009年度京都大会
  • Place of Presentation: 京都大学
• [Presentation] サイトゾル型グルタミン合成酵素欠損変異イネの幼植物における解析 (2009)
  • Author(s): 田渕真由美, ら
  • Organizer: 日本土壌肥料学会2009年度京都大会
  • Place of Presentation: 京都大学
• [Presentation] 液胞型アクアアポリンの細胞膜アンモニウム輸送への寄与 (2009)
  • Author(s): 小島創一, ら
  • Organizer: 日本土壌肥料学会2009年度京都大会
  • Place of Presentation: 京都大学
• [Presentation] イネ根におけるGlnセンサー候補OsACTPK遺伝子族の発現特性とOsACTPK4遺伝子破壊イネの解析 (2009)
  • Author(s): 澤優己, ら
  • Organizer: 日本土壌肥料学会2009年度京都大会
  • Place of Presentation: 京都大学
• [Presentation] イネ根系の発達を促進するQTL遺伝子の単離 (2009)
  • Author(s): 小原実広, ら
  • Organizer: 日本土壌肥料学会2009年度京都大会
  • Place of Presentation: 京都大学
• [Presentation] Genetic and physiological approach of nitrogen utilization toward improving environmental adaptation of rice. (2008)
  • Author(s): Obara M., Yamaya T.
  • Organizer: Workshop on Development of Environmentally-friendly Water-saving Technologies for Rice
  • Place of Presentation: Tsukuba、Japan(招待講演)
  • Year and Date: 2008-07-18
• [Presentation] Genetic and physiological approach of nitrogen utilization toward improving environmental adaptation of rice. Workshop on Development of Environmentally-friendly (2008)
  • Author(s): Obara, M., Yamaya, T.
  • Organizer: Water-saving Technologies for Rice
  • Place of Presentation: Tsukuba, Japan (招待講演)
  • Year and Date: 2008-07-18
• [Presentation] Molecular mechanisms for plant biomass production-rice as a model plant. (2008)
  • Author(s): 山谷知行
  • Organizer: UCR Tech Horizon 2008 Conference
  • Place of Presentation: UC Riverside, CA, USA(招待講演)
• [Presentation] Molecular mechanisms for plant biomass production-rice as a model plant (2008)
  • Author(s): Yamaya, T.
  • Organizer: UCR Tech Horizon 2008 Conference
  • Place of Presentation: UC Riverside, CA, USA (招待講演)
• [Presentation] Molecular mechanisms for plant biomass production -rice as a model plant (2008)
  • Author(s): Yamaya, T.
  • Organizer: UCR Tech Horizon 2008 Conference
  • Place of Presentation: UC Riverside, CA, USA
• [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice. (2007)
  • Author(s): 山谷知行
  • Organizer: Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Lancaster University, UK(招待講演)
• [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice (2007)
  • Author(s): Yamaya, T.
  • Organizer: Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Lancaster University, UK (招待講演)
• [Presentation] Assimilation of ammonium ions and re-utilization of nitrogen in rice (2007)
  • Author(s): Yamaya, T.
  • Organizer: Nitrogen2007-An International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Lancaster University, UK
• [Book] Nitrogen Assimilation in Plants(India, Ammonia absorption and assimilation in plants) (2010)
  • Author(s): Kojima K., von Wiren N., Yamaya T.
  • Publisher: Research Signpost 社
• [Book] 植物栄養学 第2版(間藤徹、馬建鋒、藤原徹編)(アンモニウムイオンの吸収、アンモニアトランの同化) (2010)
  • Author(s): 山谷知行
  • Publisher: 文永堂出版
• [Book] India, Ammonia absorption and assimilation in plants, In : "Nitrogen Assinlilation in Plants" (2010)
  • Author(s): Kojima, K., von Wiren, N., Yamaya, T.
  • Publisher: Research Signpost 社(印刷中)
• [Book] 細胞内アンモニア代謝、アンモニアトランスポーター、「植物栄養学 第2版」間藤徹, 馬建鋒, 藤原徹, 編 (2010)
  • Author(s): 山谷知行
  • Publisher: 文永堂出版(印刷中)
• [Book] Nitrogen Assimilation in Plants (2010)
  • Author(s): Kojima, et al.
  • Publisher: Research Signpost, India(印刷中)
• [Book] 植物の百科事典(b窒素代謝) (2009)
  • Author(s): 山谷知行
  • Publisher: 朝倉書店
• [Book] 植物の百科事典(1.2 物質代謝) (2009)
  • Author(s): 山谷知行
  • Publisher: 朝倉書店
• [Book] b窒素代謝、「植物の百科事典」 (2009)
  • Author(s): 山谷知行
  • Total Pages: 16
  • Publisher: 朝倉書店
• [Book] 1. 2物質代謝、「植物の百科事典」 (2009)
  • Author(s): 山谷知行
  • Publisher: 朝倉書店
• [Remarks] 報道関連情報
• [Remarks] 山谷知行、東北大学の新世紀-世界をリードするイネの研究、2009年6月29日、東日本放送
• [Remarks] アウトリーチ活動
• [Remarks] 山谷知行、植物の基盤研究から食料自給率を考える。文部科学省政策会議先端科学技術調査会「植物科学研究が切り拓く未来-グリーンイノベーションに向けて」、衆議院第1議員会館民主党A会議室、2010年3月4日
• [Remarks] ホームページ等
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