Analyses of the N-substituted formamide metabolic pathway and their applications to the production of useful compounds
| Project/Area Number |
19208008
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIGASHIBATA Hiroki 東洋大学, 生命科学部, 准教授 (20344864)
HASHIMOTO Yoshiteru 筑波大学, 大学院・生命環境科学研究科, 講師 (00323254)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2009: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2008: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2007: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
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| Keywords | N-置換ホルムアミド / イソニトリル / アミド / 代謝 / 酵素 |
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| Research Abstract |
To clarify the function of enzymes involved in the N-substituted formamide synthesis/degradation, the microorganism with both enzyme activities was analyzed. Isonitrile hydratase was purified and characterize. The enzyme was found to be a new type of isonitrile hydratase. Reverse reaction of N-substituted formamide deformylase was found and the reaction mechanism was proposed. Some compounds were produced using the reverse reaction the enzyme.
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Report
(4 results)
Research Products
(27 results)
