• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of factors implicated in pathogenicity of mononegaviruses and development of a vaccine candidate.

Research Project

Project/Area Number 19208025
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo

Principal Investigator

KAI Chieko  The University of Tokyo, 医科学研究所, 教授 (10167330)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Hiroyuki  東京大学, 農学生命科学研究科, 教授 (40155891)
YONEDA Misako  東京大学, 医科学研究所, 准教授 (40361620)
SATOU Hiroki  東京大学, 医科学研究所, 助教 (50418654)
Project Period (FY) 2007 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2009: ¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2008: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2007: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Keywordsモノネガウイルス / モービリウイルス / 病原性 / 組換えウイルス / 宿主因子 / 麻疹ウイルス / 牛疫ウイルス / イヌジステンパーウイルス / アクセサリー蛋白 / 病原性発現 / 組換えウイルスワクチン
Research Abstract

We investigated the key factors in pathogenicity of morbilliviruses, and developed a vaccine for Nipah virus infection. For morbilliviruses, it was discovered that MV induced different cellular responses in a cell-type specific mannar, and viral nucleocapsid protein had inhibitory potency for IFN-α/β and γ signaling. For Nipah virus, we showed that accessory proteins play key roles in it's pathogenicity. We have also showed that our established recombinant measles virus expressing Nipah virus protein effectively protected hamsters from NiV infection.

Report

(4 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (64 results)

All 2010 2009 2008 2007 Other

All Journal Article (18 results) (of which Peer Reviewed: 8 results) Presentation (40 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Comparative and mutational analyses of promoter regions of rinderpest virus.2010

    • Author(s)
      Imai C., Fujita K., Shimizu F., Sugai, A., Yoneda M., Kai C.
    • Journal Title

      Virology 396

      Pages: 169-177

    • Related Report
      2009 Final Research Report
  • [Journal Article] CD147/EMMPRIN acts as a functional entry receptor for measles virus on epithelial cells.2010

    • Author(s)
      Watanabe A., Yoneda M., Ikeda F., Terao-Muto Y., Sato H., Kai C.
    • Journal Title

      J.Virol. 84(9)

      Pages: 4183-4193

    • Related Report
      2009 Final Research Report
  • [Journal Article] The nonstructural proteins of Nipah virus play a key role in pathogenicity in vivo.2010

    • Author(s)
      Yoneda, M., Guillaume, V., Sato, H., Fujita, K., Georges-Courbot, M-C., Ikeda, F., Omi, M., Muto-Terao, Y., Wild, F., Kai, C.
    • Journal Title

      PLoS ONE

      Volume: 5(9)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epitope mapping of canine distemper virus phophoprotein by monoclonal antibodes.2009

    • Author(s)
      Sugai, A., Kooriyama, T., Sato, H., Yoneda, M., Kai, C.
    • Journal Title

      Microbiol Immunol.

      Volume: 53(12) Pages: 667-74

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pathological and phylogenetic features of prevalent canine distemper viruses in with Masked palm civets in Japan.2009

    • Author(s)
      Takayama, I.
    • Journal Title

      Comp. Immunol. Microb. (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of host protein synthesis in B95a cells infected with HL strain of measles virus.2008

    • Author(s)
      Inoue Y., Tshukiyama-Kohara K., Yoneda M., Sato H., Kai C.
    • Journal Title

      Comp.Immunol.Microb. 32

      Pages: 29-41

    • Related Report
      2009 Final Research Report
  • [Journal Article] Phosphorylation of measles virus nucleoprotein upregulates the transcriptional activity of minigenomic RNA.2008

    • Author(s)
      Hagiwara K., Sato H., Inoue Y., Watanabe A., Yoneda M., Ikeda F., Fujita K., Fukuda H., Takamura C., Kozuka-Hata H., Oyama M., Sugano S., Ohmi S., Kai C.
    • Journal Title

      Proteomics 8

      Pages: 1871-1879

    • Related Report
      2009 Final Research Report
  • [Journal Article] Measles virus induced cell-type specific changes in gene expression2008

    • Author(s)
      Sato H., Honma R., Yoneda M., Miura R., Tsukiyama-Kohara K., Ikeda F., Seki T., Watanabe S., Kai C.
    • Journal Title

      Virology 375

      Pages: 321-330

    • Related Report
      2009 Final Research Report
  • [Journal Article] Heparin-like glycosaminoglycans prevent the infection of measles virus in SLAM-negative cell lines.2008

    • Author(s)
      Terao-Muto Y., Yoneda M., Seki T., Watanabe A., Tsukiyama-Kohara K., Fujita K., Kai C.
    • Journal Title

      Antiviral Res. 80

      Pages: 370-376

    • Related Report
      2009 Final Research Report
  • [Journal Article] Immune responses against measles virus in cynomolgus monkeys.2008

    • Author(s)
      Sato H., Kobune, F., Ami, Y., Yoneda M., Kai C.
    • Journal Title

      Comp.Immunol.Microb. 31(1)

      Pages: 25-35

    • Related Report
      2009 Final Research Report
  • [Journal Article] Inhibition of host protein synthesis in B95a cells infected with HL strain of measles2008

    • Author(s)
      Inoue, Y.
    • Journal Title

      Comp. Immunol. Microb. 32

      Pages: 29-41

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of measles virus nucleoprotein upregulates the transcriptional activity of minlgenomic RNA.2008

    • Author(s)
      Hagiwara, K.
    • Journal Title

      Proteomics 8

      Pages: 1871-1879

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measles virus induced cell-type Specific changes in gene expression.2008

    • Author(s)
      Sato, H
    • Journal Title

      Virology 375

      Pages: 321-330

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heparin-like glycosaminoglycans prevent the infection of measles virus in SLAM-negative cell lines.2008

    • Author(s)
      Terao-Muto, Y
    • Journal Title

      Antiviral Res. 80

      Pages: 370-376

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prior immunization with SARS-CoV nucleocapsid protein causes severe pneumonia in mice infected with SARS-CoV.2008

    • Author(s)
      Yasui, F.
    • Journal Title

      J. Immunol. 18

      Pages: 2337-48

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel monolayer cell line derived from human umbilical cord blood cells shows high sensitivity to measles virus.2007

    • Author(s)
      Kobune F., Ami Y., Katayama M., Takahashi M., Tuul R., Korukluoglu G., Kiyohara T., Miura R., Sato H., Yoneda M., Kai C.
    • Journal Title

      J.Gen.Virol. 88

      Pages: 1565-1567

    • Related Report
      2009 Final Research Report
  • [Journal Article] Host range and receptor utilization of canine distemper virus analyzed by recombinant viruses : involvement heparin-like molecule in CDV infection.2007

    • Author(s)
      Fujita K., Miura R., Yoneda M., Shimizu F., Sato H., Muto Y., Endo Y., Tsukiyama-Kohara K., Kai C.
    • Journal Title

      Virology 359

      Pages: 324-335

    • Related Report
      2009 Final Research Report
  • [Journal Article] Measles virus N protein inhibits host translation by binding to eIF3-p40.2007

    • Author(s)
      Sato H., Masuda M., Kanai M., Tsukiyama-Kohara K., Yoneda M., Kai C.
    • Journal Title

      J.Virol. 81(21)

      Pages: 11569-11576

    • Related Report
      2009 Final Research Report
  • [Presentation] ウイルス感染症における非人霊長類実験の必要性2009

    • Author(s)
      甲斐知惠子
    • Organizer
      京都大学ウイルス学研究所シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2009-02-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Pathological and phylogenetic features of prevalent canine distemper virus in wild masked palm civets in afukushima prefecture.2009

    • Author(s)
      Takayama I., Kubo M., Takenaka A., Fujita K., Sugiyama T., Arai T., Yoneda M., Sato H., Yanai T., Kai C.
    • Organizer
      1^<st> Symposium of the IMSUT & RCAST Global COE-Ancient Scourges and Modern Perspectives
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-02-10
    • Related Report
      2009 Final Research Report
  • [Presentation] Pathological and phylogenetic features of prevalent canine distemper virus in wild masked palm civets in afukushima prefecture.2009

    • Author(s)
      Takayama, I.
    • Organizer
      1st Symposium of the IMSUT & RCAST Global COE-Ancient Scourges and Modern Perspectives.
    • Place of Presentation
      Tokyo
    • Year and Date
      2009-02-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] ニパウイルスアクセサリー蛋白の病原性への関与2009

    • Author(s)
      米田美佐子、佐藤宏樹、藤田賢太郎、寺尾由里、池田房子、小見美央、甲斐知恵子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      東京
    • Related Report
      2009 Final Research Report
  • [Presentation] 麻疹N結合宿主蛋白の同定と機能解析2009

    • Author(s)
      渡邉彰、米田美佐子、佐藤宏樹、池田房子、甲斐知惠子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] モービリウイルスアクセサリーC蛋白による宿主遺伝子群発現維持機構の解明2009

    • Author(s)
      佐藤宏樹、米田美佐子、本間玲子、池田房子、渡邉慎哉、甲斐知惠子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] 蛍光融合蛋白を用いたニパウイルスN蛋白のP蛋白相互作用領域の解明2009

    • Author(s)
      小見-古谷美央、米田美佐子、池田房子、甲斐知惠子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] ニパウイルスヌクレオ蛋白のリン酸化部位の同定及びリン酸化の意義2009

    • Author(s)
      黄明珠、佐藤宏樹、萩原恭二、渡邉彰、池田房子、秦裕子、尾山大明、米田美佐子、甲斐知惠子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] 麻疹ウイルスNタンパク質のインターフェロンシグナル伝達経路への関与2009

    • Author(s)
      高山郁代、佐藤宏樹、甲斐知惠子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis of comprehensive downregulation of housekeeping genes triggered by morbillivirus infection.2009

    • Author(s)
      佐藤宏樹、米田美佐子、本間玲子、渡邉慎哉、甲斐知惠子
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] The nucleocapsid protein of morbillivirus blocks host interferon response.2009

    • Author(s)
      高山郁代、佐藤宏樹、甲斐知惠子
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification of a host factor binding to measles virus nucleoprotein.2009

    • Author(s)
      Watanabe A., Yoneda M., Sato H., Ikeda, F., Kai C.
    • Organizer
      The 9^<th> Awaji International Forum on Infection and Immunity
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis of phosphorylation residues on Nipah virus nucleoprotein.2009

    • Author(s)
      Huang M., Sato H., Hagiwara K., Watanabe A., Kozuka-Hata H., Oyama M., Yoneda M., Kai C.
    • Organizer
      The 9^<th> Awaji International Forum on Infection and Immunity
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis of phosphorylation residues on Nipah virus nucleoprotein.2009

    • Author(s)
      Huang, M., Sato, H., Hagiwara, K., Watanabe, A., Kozuka-Hata, H., Oyama, M., Yoneda, M., Kai, C.
    • Organizer
      The 9th Awaji International Forum
    • Place of Presentation
      淡路夢舞台国際会議場
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規麻疹ウイルスN結合宿主蛋白の機能解析2008

    • Author(s)
      寺尾由里、渡辺彰、米田美佐子、甲斐知惠子
    • Organizer
      第31回日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Moleculat determinant of Nipah virus pathogenicity. Symposium : "Emerging Infections : A tribute to the One Medicine2008

    • Author(s)
      甲斐知惠子
    • Organizer
      One Health Concept
    • Place of Presentation
      Manhattan KS, U.S.A.(Invited speaker)
    • Related Report
      2009 Final Research Report
  • [Presentation] 麻疹N結合宿主蛋白の同定2008

    • Author(s)
      寺尾(武藤)由里、渡邉彰、米田美佐子、甲斐知惠子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 上皮系細胞における麻疹ウイルス侵入経路の解析2008

    • Author(s)
      渡邉彰、米田美佐子、佐藤宏樹、池田房子、寺尾(武藤)由里、甲斐知恵子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] 牛疫ウイルスのpromoter領域の解析2008

    • Author(s)
      藤田賢太郎、今井千恵子、米田美佐子、甲斐知恵子
    • Organizer
      第56回日本ウイルス学会
    • Place of Presentation
      岡山
    • Related Report
      2009 Final Research Report 2008 Annual Research Report
  • [Presentation] Comparative and mutational analysis of promoter regions of rinderpest virus.2008

    • Author(s)
      Fujita K., Imai C., Yoneda M., Kai C.
    • Organizer
      The 8^<th> Awaji International Forum on Infection and Immunity
    • Related Report
      2009 Final Research Report
  • [Presentation] Inhibitory effect of heparin on infection of SLAM-negative cells with measles virus.2008

    • Author(s)
      Terao-Muto Y., Yoneda M., Watanabe A., Fujita K., Kai C.
    • Organizer
      The 8^<th> Awaji International Forum on Infection and Immunity
    • Related Report
      2009 Final Research Report
  • [Presentation] The function of Nipah virus accessory protein as virulence factors in vivo.2008

    • Author(s)
      Yoneda M., Gillaume V., Sato H., Georges-Courbot M-C., Takayama I., Ikeda F., Wild F., 甲斐知惠子
    • Organizer
      XIVth Int.Cong.Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2009 Final Research Report
  • [Presentation] Phosphorylation of measles virus nucleoprotein upregulates the transcriptional activity of minigenomic RNA.2008

    • Author(s)
      Hagiwara, Sato H., Inoue Y., Watanabe A., Yoneda M., Ikeda F., Fujita K., Kai C.
    • Organizer
      XIVth Int.Cong.Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2009 Final Research Report
  • [Presentation] Translation of measles virus nucleocapsid mRNA is upregulated by a host RNA binding protein.2008

    • Author(s)
      Inoue Y., Tsukiyama-Kohara K., Fujita K., Hagiwara K., Sato H., Yoneda M., Kai C.
    • Organizer
      XIVth Int.Cong.Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2009 Final Research Report
  • [Presentation] A functional entry receptor for measles virus in epithelial cells.2008

    • Author(s)
      Watanabe A., Yoneda M., Terao-Muto Y., Sato H., Kai C.
    • Organizer
      XIVth Int.Cong.Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2009 Final Research Report
  • [Presentation] Analysis on proteins of measles virus required for neuropathogenesis in mice.2008

    • Author(s)
      Arai T., Terao-Muto Y., Uema A., Ikeda, F., Yoneda M., Kai C.
    • Organizer
      XIVth Int.Cong.Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2009 Final Research Report
  • [Presentation] The function of Nipah virus accessory protein as virulence factors in vivo.2008

    • Author(s)
      Yoneda, M.
    • Organizer
      XIVth Int. Cong. Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2008 Annual Research Report
  • [Presentation] Phosphorylation of measles virus nucleoprotein upregulates the transcriptional activity of minigenomic RNA.2008

    • Author(s)
      Hagiwara, K.
    • Organizer
      XIVth Int. Cong. Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2008 Annual Research Report
  • [Presentation] Translation of measles virus nucleocapsid mRNA is upregulated by a host RNA binding protein.2008

    • Author(s)
      Inoue, Y
    • Organizer
      XIVth Int. Cong. Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2008 Annual Research Report
  • [Presentation] A funvtional entry receptor for measles virus in epithelial cells.2008

    • Author(s)
      Watanabe, A.
    • Organizer
      XIVth Int. Cong. Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2008 Annual Research Report
  • [Presentation] Analysis on proteins of measles virus required for neuropathogenesis in mice.2008

    • Author(s)
      Arai, T.
    • Organizer
      XIVth Int. Cong. Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2008 Annual Research Report
  • [Presentation] Effects on a measles virus vector targeting breast cancer cells by expression of single chain antibody against rat HER-2/neu.2008

    • Author(s)
      Sugiyama, T.
    • Organizer
      XIVth Int. Cong. Virol.
    • Place of Presentation
      Istanbul, Turkey
    • Related Report
      2008 Annual Research Report
  • [Presentation] Comparative and mutational analysis of promoter regions of rinderpest virus.2008

    • Author(s)
      Fujita, K.
    • Organizer
      The 8th Awaji International Forum on Infection and Immunlty
    • Place of Presentation
      Hyogo, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] Inhibitory effect of heparin on infection of SLAM-negative cells with measles virus.2008

    • Author(s)
      Terao-Muto, Y
    • Organizer
      The 8th Awaji International Forum on Infection and Immunlty
    • Place of Presentation
      Hyogo, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] ヒトリンパ球移入NOD/SCIDマウス(huPBL NOD/SCID)を用いた組換麻疹ウイルス評価系の構築2008

    • Author(s)
      佐藤正明
    • Organizer
      日本ウイルス学会九州支部会
    • Place of Presentation
      熊本
    • Related Report
      2008 Annual Research Report
  • [Presentation] Moleculat determinant of Nipah virus pathogenicity.2008

    • Author(s)
      Kai, C.
    • Organizer
      Symposium: "Emerging Infections: A tribute to the One Medicine, One Health Concept"
    • Place of Presentation
      Manhattan KS, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Nipah virus reverse genetics.2007

    • Author(s)
      甲斐知惠子
    • Organizer
      5^<th>Int.Conf.on Emerging zoonoses
    • Place of Presentation
      Limassol, Cyprus. (Invited speaker)
    • Related Report
      2009 Final Research Report
  • [Presentation] 麻疹ウイルス齧歯類脳馴化株を用いた組換えウイルスのマウスにおける神経病原性2007

    • Author(s)
      新井哲郎、武藤由里、上間亜希子、池田房子、米田美佐子、甲斐知惠子
    • Organizer
      第55回日本ウイルス学会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] 麻疹ウイルス感染におけるheparinの機能的関与2007

    • Author(s)
      武藤由里、藤田賢太郎、米田美佐子、甲斐知惠子
    • Organizer
      第55回日本ウイルス学会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Presentation] 麻疹ウイルスヌクレオカプシドタンパク質のリン酸化部位の同定2007

    • Author(s)
      萩原恭二、井上義久、藤田賢太郎、渡辺彰、佐藤宏樹、甲斐知惠子
    • Organizer
      第55回日本ウイルス学会
    • Place of Presentation
      札幌
    • Related Report
      2009 Final Research Report
  • [Book] 臨床と微生物(ニパウイルス,ヘンドラウイルス(特集:種の壁を越える感染症-EpidemiologyとEpizootiology-) 37(2))2010

    • Author(s)
      米田美佐子、甲斐知恵子
    • Publisher
      近代出版
    • Related Report
      2009 Final Research Report
  • [Book] Virus Report(ジステンパーウイルス(特集:動物のウイルス感染症) 6)2009

    • Author(s)
      佐藤宏樹、甲斐知恵子
    • Related Report
      2009 Final Research Report
  • [Book] ジステンパーウイルス(特集:動物のウイルス感染症)2009

    • Author(s)
      佐藤宏樹、甲斐知恵子
    • Publisher
      Virus Report
    • Related Report
      2009 Annual Research Report
  • [Book] Methods in Molecular Biology, "Viral Applications of the Green Fluorescent Protein"(Reverse genetics of Nipah virus to probe viral pathogenicity.)2008

    • Author(s)
      Yoneda M., Fujita K., Sato H., Kai C.
    • Publisher
      The Humana Press
    • Related Report
      2009 Final Research Report
  • [Book] Reverse genetics of Nipah virus to probe viral pathogenicity. In Methods in Molecular Biology, "Viral Applications of the Green Fluorescent Protein"2008

    • Author(s)
      Yoneda, M
    • Total Pages
      357
    • Publisher
      The Humana Press
    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.ims.u-tokyo.ac.jp/jikkendoubutsu/index.htm

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi