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Developments of transmission-blocking vaccine for tick-borne protozoan diseases

Research Project

Project/Area Number 19208026
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionKagoshima University

Principal Investigator

FUJISAKI Kouzo  Kagoshima University, 農学部, 教授 (00292095)

Co-Investigator(Kenkyū-buntansha) TANAKA Tetsuya  鹿児島大学, 農学部, 准教授 (00322842)
SUZUKI Hiroshi  帯広畜産大学, 原虫病研究センター, 教授 (60333473)
TSUJI Naotoshi  独立行政法人農業食品産業技術総合研究機構, 動物衛生研究所, 主任研究員 (70355171)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2008: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2007: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Keywordsマダニ / Babesia原虫 / マダニ生物活性分子 / TBM / アミノ酸 / TOR / オートファジー / ワクチン / TOR情報伝達経路 / BD-TBM / TORシグナル伝達機構 / S6K / GATA / TBV / TOR経路 / リボソーム / 細胞内バイオマス / リジン分解 / 多重ビテロジェニン
Research Abstract

(1) We could identify for the first time vitellogenins (Vg), vitellogeni-receptor (VgR), and longipain as tick-bioactive molecules (TBM) specifically reacted with sexual and sporogonic stages (S-S satages) of Babesia parasites developing in the vector ticks. (2) Since Vg and VgR could inhibit completely the Babesia transmission in ticks treated with their dsRNA and could suppress effectively the Babesia transmission in animals vaccinated with them, the identification of Babesia molecules reacted with them is now concentrated. (3) However, longipain could promote Babesia development in vector ticks after RNAi, it is considered that its application as candidate molecule for the transmission-blocking vaccine may be difficult under the current research situation lacking of the TBM over0expression systems. (4) During the period of this research, we started to focus on TBMs involved in cellular biomass (CB)control in host cells for Babesia parasites because it was noticed that the development of intra-cellular parasites is fundamentally influenced by the essential nutrients supply under the control of CB. (5) Therefore, the molecular identification and characterization of a series of TBMs involving in transcription, translation, post translational modification, and vesicular transport, autophagy (AT) of intra-cellular proteins are concentrated for the first time. (6) These studies are elucidating the significance of TOR pathways (TORs) as candidate molecules for the transmission-blocking vaccine.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (91 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (57 results) (of which Peer Reviewed: 56 results) Presentation (21 results) Book (3 results) Remarks (6 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Longistatin, a plasminogen activator, is key to the availability of blood meals for ixodid ticks.2011

    • Author(s)
      Anisuzzman, Islam MK, Abdul MA, Miyoshi T, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N
    • Journal Title

      PLoS Pathogen 第7巻

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of class B scavenger receptor CD36 from the hard tick Haemaphysalis longicornis.2011

    • Author(s)
      Aung KM, Boldbaatar D, Liao M, Nakao S, Umeimiya-Shirafuji R, Tanaka T, Fujisaki K
    • Journal Title

      Parasitol. Res. 第108巻

      Pages: 273-285

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The identification and characterization of lysozyme from the hard tick Haemaphysalis longicornis.2010

    • Author(s)
      Tanaka T, Kawano S, Nakao S, Umemiya-Shirafuji R, Rahman M M, Moldbaatar D, Battur B, Liao M, Fujisaki K
    • Journal Title

      Ticks Tickborne Dis. 第1巻

      Pages: 178-185

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development.2010

    • Author(s)
      Boldbaatar D, Battur B, Umemiya-Shirafuji R, Liao M, Tanaka T, Xuan X, Fujisaki K
    • Journal Title

      J.Insect Physiol. 第56巻

      Pages: 1587-1598

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy and its physiological relevance in arthropods : Current knowledge and perspectives.2010

    • Author(s)
      Malagoli D, Abdalla FC, Cao Y, Feng Q, Fujisaki K, Gregorc A, Matsuo T, Nezis IP, Papassideri IS, Sass M, Silva-Zacarin ECM, Tettamanti G, Umemiya-Shirafuji R
    • Journal Title

      Autophagy 第6巻

      Pages: 575-588

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Hlcyst-3 as a member of cystatins from the tick Haemaphysalis longicornis.2010

    • Author(s)
      Zhou J, Liao M, Gong H, Xuan X, Fujisaki K
    • Journal Title

      Exp.Appl.Acarol. 第51巻

      Pages: 327-333

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes.2010

    • Author(s)
      Hatta T, Tsuji N, Miyoshi T, Islam MK, Alim MA, Yamaji K, Anisuzzaman, Fujisaki K
    • Journal Title

      Parasitol Int. 第59巻

      Pages: 286-289

    • NAID

      10029824590

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased expression of ATG genes during nonfeeding periods in the tick Haemaphysalis longicornis.2010

    • Author(s)
      Umemiya-Shirafuji R, Matsuo T, Liao M, Boldbaatar D, Battur B, Suzuki H, Fujisaki K
    • Journal Title

      Autophagy. 第6巻

      Pages: 473-481

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Parasiticidal activity of human α-edefensin-5 against Toxoplasma gondii.2010

    • Author(s)
      Tanaka T, Rahman MM, Battur B, Boldbaatar D, Liao M, Umemiya-Shirafuji R, Xuan X, Fujisaki K
    • Journal Title

      In Vitro Cell.Dev.Biol. 第46巻

      Pages: 560-565

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hlcyst-1 and Hlcyst-2 Are Potential Inhibitors of HlCPL-A in the Midgut of the Ixodid Tick Haemaphysalis longicornis.2010

    • Author(s)
      Yamaji K, Tsuji N, Miyoshi T, Hatta T, Alim MA, Anisuzzaman, Kushibiki S, Fujisaki K
    • Journal Title

      J.Vet.Med.Sci. 第72巻

      Pages: 599-604

    • NAID

      130000168010

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Kunitz-type proteinase inhibitor from the midgut of the ixodid tick, Haemaphysalis longicornis, and its endogenous target serine proteinase.2010

    • Author(s)
      Tsuji N, Islam MK, Alim MA, Hatta T, Yamaji K, Anisuzzaman, Fujisaki K
    • Journal Title

      Mol Biochem Parasitol. 第170巻

      Pages: 112-115

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] GATA transcription, translation and regulation in Haemaphysalis longicornis tick : analysis of the cDNA and essential role for vitellogenesis.2010

    • Author(s)
      Boldbaatar D, Battur B, Umemiya-Shirafuji R, Liao M, Tanaka T, Xuan X, Fujisaki K
    • Journal Title

      Insect Biochem. Mol.Biol. 第40巻

      Pages: 49-57

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Longistatin, a novel EF-hand protein from the ixodid tick Haemaphysalis longicornis, is required for acquisition of host blood-meals.2010

    • Author(s)
      Anisuzzaman, Islam MK, Miyoshi T, Alim MA, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N
    • Journal Title

      Int J Parasitol. 第40巻

      Pages: 721-729

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural characterization and cytolytic activity of a potent antimicrobial motif in longicin, a defensin-like peptide in the tick Haemaphysalis longicornis.2010

    • Author(s)
      Rahman M, Tsuji N, Boldbaatar D, Battur B, Liao M, Umemiya-Shirafuji R, You M, Tanaka T, Fujisaki K
    • Journal Title

      J.Vet.Med.Sci. 第72巻

      Pages: 149-156

    • NAID

      130000134519

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased expression of ATG genes during nonfeeding periods in the tick Haemaphysalis longicornis2010

    • Author(s)
      Umemiya-Shirafuji R
    • Journal Title

      Autophagy

      Volume: 6 Pages: 473-481

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GATA transcription, translation and regulation in Haemaphysalis longicornis tick : Analysis of the cDNA and an essential role for vitellogenesis2010

    • Author(s)
      Boldbaatar D
    • Journal Title

      Insect Biochemical and Molecular Biology

      Volume: 40 Pages: 49-57

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development2010

    • Author(s)
      Boldbaatar D
    • Journal Title

      Journal of Insect Physiology

      Volume: 56 Pages: 1587-1598

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hemalin, a thrombin inhibitor isolated from a midgut cDNA library from the hard tick Haemaphysalis longicornis.2009

    • Author(s)
      Liao M, Zhou J, Gong H, Boldbaatar D, Shirafuji-Umemiya R, Battur B, Dong J, Nishikawa Y, Fujisaki K
    • Journal Title

      J.Insect Physiol. 第55巻

      Pages: 165-174

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis.2009

    • Author(s)
      Zhou J, Liao M, Ueda M, Gong H, Xuan X, Fujisaki K
    • Journal Title

      Vet.Parasitol. 第160巻

      Pages: 180-183

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Blocking the secretion of saliva by silencing the HlYkt gene in the tick Haemaphysalis longicornis.2009

    • Author(s)
      Gong H, Umemiya R, Zhou J, Liao M, Zhang H, Jia H, Nishikawa Y, Xuan X, Fujisaki K
    • Journal Title

      Insect Biochem. Mol.Biol. 第39巻

      Pages: 372-381

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The hard tick Kunitz inhibitor, Haemangin, is a novel modulator or angiogenesis and wound healing and is crucial for slower blood-feeding arthropods.2009

    • Author(s)
      Islam MK, Tsuji N, Miyoshi T, Alim MA, Huang X, Hatta T, Fujisaki K
    • Journal Title

      PLoS Pathogen 第5巻

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] LKR/SDH plays important roles throughout the tick life cycle including a long starvation period.2009

    • Author(s)
      Battur B, Boldbaatar D, Umemiya-Shirafuji R, Liao M, Battsetseg B, Baymbaa B, Fujisaki K
    • Journal Title

      PLoS One 第4巻

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hemoglobinase activity of a cysteine protease from the ixodid tick Haemaphysalis longicornis.2009

    • Author(s)
      Yamaji K, Tsuji N, Miyoshi T, Islam MK, Hatta T, Alim MA, Anisuzzaman, Takenaka A, Fujisaki K.
    • Journal Title

      Parasitol. Int. 第58巻

      Pages: 232-237

    • NAID

      10027669546

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and molecular characterization of tick kynureine aminotransferase (HlKAT) from Haemaphysalis longicornis (Acari : Ixodidae).2009

    • Author(s)
      Battsetseg B, Boldbaatar D, Battur B, Xuan X, Fujisaki K
    • Journal Title

      Prasitol. Res. 第105巻

      Pages: 669-679

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis (Acari : Ixodiae).2009

    • Author(s)
      You M, Fujisaki K
    • Journal Title

      J.Vet.Med.Sci. 第71巻

      Pages: 709-712

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hemalin, a thrombin inhibitor isolated from a midgut cDNA library from the hard tick Haemaphysalis longicornis2009

    • Author(s)
      Liao M, Zhou J, Gong H, Boldbaatar D, Shirafuji-Umemiya R, Battur B, Dong J, Nishikawa Y, Fujisaki K
    • Journal Title

      J.Insect Physiol. 55

      Pages: 165-174

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis2009

    • Author(s)
      Zhou J, Liao M, Ueda M, Gong H, Xuan X, Fujisaki K
    • Journal Title

      Vet.Parasitol. 160

      Pages: 180-183

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Blocking the secretion of saliva by silencing the HlYkt gene in the tick Haemaphysalis longicornis2009

    • Author(s)
      Gong H, Umemiya R, Zhou J, Liao M, Zhang H, Jia H, Nishikawa Y, Xuan X, Fujisaki K
    • Journal Title

      Insect Biochem.Mol.Biol. 39

      Pages: 372-381

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The hard tick Kunitz inhibitor, Haemangin, is a novel modulator or angiogenesis and wound healing and is crucial for slower blood-feeding arthropods2009

    • Author(s)
      Islam MK, Tsuji N, Miyoshi T, Alim MA, Huang X, Hatta T, Fujisaki K
    • Journal Title

      PLoS Pathogen 5

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] LKR/SDH plays important roles throughout the tick life cycle including a long starvation period2009

    • Author(s)
      Battur B, Boldbaatar D, Umemiya-Shirafuji R, Liao M, Battsetseg B, Baymbaa B, Fujisaki K
    • Journal Title

      PLoS One 4

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Hemoglobinase activity of a cysteine protease from the ixodid tick Haemaphysalis longicornis2009

    • Author(s)
      Yamaji K, Tsuji N, Miyoshi T, Islam MK, Hatta T, Alim MA, Anisuzzaman, Takenaka A, Fujisaki K.
    • Journal Title

      Parasitol.Int. 58

      Pages: 232-237

    • NAID

      10027669546

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Cloning and molecular characterization of tick kynureine aminotransferase (HlKAT) from Haemaphysalis longicornis (Acari : Ixodidae)2009

    • Author(s)
      Battsetseg B, Boldbaatar D, Battur B, Xuan X, Fujisaki K
    • Journal Title

      Prasitol.Res. 105

      Pages: 669-679

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis (Acari : Ixodiae)2009

    • Author(s)
      You M, Fujisaki K
    • Journal Title

      J.Vet.Med.Sci. 71

      Pages: 709-712

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The hard tick Kunitz inhibitor, Haemangin, is a novel modulator or angiogenesis and wound healing and is crucial for slower blood-feeding arthropods2009

    • Author(s)
      Islam MK
    • Journal Title

      PLoS Pathogen. 5

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LKR/SDH plays important roles throughout the tick life cycle in hiding a long starvation period2009

    • Author(s)
      Battur B
    • Journal Title

      PLoS One 4

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blocking the secretion of saliva by silencing the HIYkt gene in the tick Haemaphysalis longicornis2009

    • Author(s)
      Gong H
    • Journal Title

      Insect Biochemical and Molecular Biology 39

      Pages: 372-381

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      今井壮一, ほか3名
    • Journal Title

      図説獣医衛生動物学(講談社)

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Vaccination effects of recombinant chitinase protein from the hard tick Haemaphysalis longicornis(Acari : Ixodiae)2009

    • Author(s)
      You M
    • Journal Title

      J.Vet.Med.Sci. 71

      Pages: 709-712

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tick vitellogenin receptor reveals critical role in oocyte development and transovarial transmission of Babesia parasite.2008

    • Author(s)
      Boldbaatar B, Battsetseg B, Matuso T, Hatta T, Umemiya-Shirafuji R, Xuan X, Fujisaki K
    • Journal Title

      Biochem. Cell Biol. 第86巻

      Pages: 331-344

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy in ticks.2008

    • Author(s)
      Umemiya-Shirafuji R, Matsuo T, Fujisaki K
    • Journal Title

      Meth.Enzymol. 第451巻

      Pages: 621-638

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Developmental stage- and organ-specific expression profiles of asparaginyl endopeptidases/legumains in the ixodid tick Haemaphysalis longicornis.2008

    • Author(s)
      Alim MA, Tsuji N, Miyoshi T, Islam MK, Hatta T, Fujisaki K
    • Journal Title

      J.Vet.Med.Sci. 第70巻

      Pages: 1363-1366

    • NAID

      110007021720

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A set of serine proteinase paralogs are required for blood-digestion in the ixodid tick Haemaphysalis longicornis.2008

    • Author(s)
      Miyoshi T, Tsuji N, Islam MK, Alim MA, Hatta T, Huang X, Fujisaki K
    • Journal Title

      Parasitol. Int. 第57巻

      Pages: 499-505

    • NAID

      10025329547

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of two forms of Cyclophilin from the hard tick Haemaphysalis longicornis.2008

    • Author(s)
      Boldbaatar D, Musyoka KR, Battur B, Umemiya R, Tanaka T, Xuan X, Fujisaki K
    • Journal Title

      Process Bicohem. 第43巻

      Pages: 615-625

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A cysteine protease is critical for Babesia spp. Transmission in Haemaphysalis ticks.2008

    • Author(s)
      Tsuji N, Miyoshi T, Battstseg B, Matuo T, Xuan X, Fujisaki K
    • Journal Title

      PLoS Pathogen 第4巻

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.2008

    • Author(s)
      Klionsky DJ, ……Fuisaki K……, et al
    • Journal Title

      Autophagy 第4巻

      Pages: 151-175

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] HlLgm2, a member of asparaginyl endopeptidases/legumains in the midgut of the ixodid tick Haemaphysalis longicornis, is involved in blood-meal digestion.2008

    • Author(s)
      Alim MA, Tsuji N, Miyoshi T, Islam MK, Huang X, Hatta T, Fujisaki K
    • Journal Title

      J.Insect Physiol. 第54巻

      Pages: 573-585

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of protein disulfide isomerases in blood feeding, viability and oocyte development in Haemaphysalis longicornis ticks Insect Biochem.2008

    • Author(s)
      Liao M, Boldbaatar D, Gong H, Huang P, Umemiya R, Harnnoi T, Zhou J, Tanaka T, Suzuki H, Xuan X, Fujisaki K
    • Journal Title

      Mol.Biol. 第38巻

      Pages: 285-295

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gene silencing of ribosomal protein P0 is lethal to the tick Haemaphysalis longicornis.2008

    • Author(s)
      Gong H, Liao M, Zhou J, Hatta T,Huan P, Zhang G, Kanuka H, Nishikawa Y, Xuan X, Fujisaki K
    • Journal Title

      Vet.Parasitol. 第151巻

      Pages: 268-278

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy- related genes from a tick, Haemaphysalis longicornis.2008

    • Author(s)
      Umemiya R, Matsuo T, Hatta T, Sakakibara S, Boldbaatar D, Fujisaki K
    • Journal Title

      Autophagy 第4巻

      Pages: 79-81

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tick vitellogenin receptor reveals critical role in oocyte development and transovarial transmission of Babesia parasite2008

    • Author(s)
      Boldbaatar B, Battsetseg B, Matuso T, Hatta T, Umemiya-Shirafuji R, Xuan X, Fujisaki K
    • Journal Title

      Biochem.Cell Biol. 86

      Pages: 331-344

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Autophagy in ticks2008

    • Author(s)
      Umemiya-Shirafuji R, Matsuo T, Fujisaki K
    • Journal Title

      Meth.Enzymol. 451

      Pages: 621-638

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Autophagy in ticks2008

    • Author(s)
      Umemiya-Shirafuji R
    • Journal Title

      Meth.Enzymol. 451

      Pages: 621-638

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy-related genes from a tick, Haemaphysalis longicornis2008

    • Author(s)
      Umemiya-Shirafuji R
    • Journal Title

      Autophagy 4

      Pages: 79-81

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A cysteine protease is critical for Babesia spp.Transmission in Haemaphysalis ticks2008

    • Author(s)
      Tsuji N
    • Journal Title

      PLoS Pathog. 4

      Pages: 1-14

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Haemaphysalis longicornis: Molecular characterization of a homologue of the macrophage migration inhibitory factor from the partially fed ticks.2007

    • Author(s)
      Umemiya R
    • Journal Title

      Exp. Parasitol. 115

      Pages: 135-142

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and characterization of an autophagy-related gene, ATG12, from the three-host tick Haemaphysalis longicornis.2007

    • Author(s)
      Umemiya R
    • Journal Title

      Insect Biochem. Mol. Biol. 37

      Pages: 975-984

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Autophagy-related genes from a tick, Haemaphysalis longicornis.2007

    • Author(s)
      Umemiya R
    • Journal Title

      Autophagy 4

      Pages: 79-81

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] マダニの原虫媒介に血液消化酵素が果たす役割2010

    • Author(s)
      藤崎幸藏
    • Organizer
      第22回日本比較免疫学会
    • Place of Presentation
      九州大学
    • Year and Date
      2010-08-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] マダニの吸血消化の分子基盤 : 最近の話題2010

    • Author(s)
      藤崎幸蔵
    • Organizer
      第24回日本動物原虫病学会学術集会
    • Place of Presentation
      日本生命科学大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 原虫病の原状とその制御の困難性2010

    • Author(s)
      藤崎幸蔵
    • Organizer
      第73回家畜衛生学会家畜衛生フォーラム
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] Molecular interface between Babesia parasites and tick vitellogenesis associated molecules, especially vitellogenin receptor2010

    • Author(s)
      藤崎幸蔵
    • Organizer
      International Seminar of Zhejiang University
    • Place of Presentation
      Hangzou, China
    • Related Report
      2010 Final Research Report
  • [Presentation] マダニの原虫媒介に血液消化酵素が果たす役割2010

    • Author(s)
      藤崎幸蔵
    • Organizer
      第22回日本比較免疫学会
    • Place of Presentation
      福岡
    • Related Report
      2010 Final Research Report
  • [Presentation] Longistatin, a potent salivary-gland plasminoge activator from ixodid tick, is critical for blood pool development and successfur blood-feeding by the ticks2010

    • Author(s)
      Anisuzzaman, Islam MK, Miyoshi T, Alim MA, Hatta T, Matsumoto Y, Fujisaki K, Tsuji N
    • Organizer
      12th International Congress of Parasitology
    • Place of Presentation
      オーストラリア
    • Related Report
      2010 Final Research Report
  • [Presentation] Asparaginyl endopeptidases/legumains are critically essential for blood-feeding, midgut cellular remodelling and reproduction in the ixodid tick Haemaphysalis longicornis2010

    • Author(s)
      Alim MA, Tsuji N, Miyoshi T, Islam MK, Hatta T, Anisuzzaman, Fujisaki K
    • Organizer
      12th International Congress of Parasitology
    • Place of Presentation
      オーストラリア
    • Related Report
      2010 Final Research Report
  • [Presentation] Molecular characterization and expression analysis of lysozome gene from the hard tick Haemaphysalis longicornis2010

    • Author(s)
      Tanaka T, Kawano S, Nakao S, Umemiya-Shirafuji R, Rahman M, Boldbaatar D, Battur B, Liao M, Fujisaki K
    • Organizer
      12th International Congress of Parasitologu
    • Place of Presentation
      オーストラリア
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification and characterization of Forkhead traqnscription factor from the hard tick, Haemaphysalis longicornis2010

    • Author(s)
      Boldbaatar D, 白藤(梅宮)梨可, 田仲哲也, 松岡輝重, 藤崎幸蔵
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本生命科学大学
    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
  • [Presentation] RNA干渉法によるフタトゲチマダニTOR様遺伝子の発現抑制2010

    • Author(s)
      白藤(梅宮)梨可, Boldbaatar D, Liao M, 藤崎幸蔵
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本生命科学大学
    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
  • [Presentation] マダニの吸血消化の分子基盤:最近の話題2010

    • Author(s)
      藤崎幸蔵
    • Organizer
      第24回日本動物原虫病学会学術集会
    • Place of Presentation
      日本生命科学大学
    • Related Report
      2010 Final Research Report
  • [Presentation] Role of SNARE protein in the secretion cascade f tick saliva2009

    • Author(s)
      Fujisaki K
    • Organizer
      13^<th> International Congress of Protistology
    • Place of Presentation
      Bujios, Brazil
    • Year and Date
      2009-08-25
    • Related Report
      2008 Annual Research Report
  • [Presentation] マダニのオートファジー研究の展開 : マダニとその媒介病原体の制圧に向けて2009

    • Author(s)
      白藤(梅宮)梨可, 松尾智英, 藤崎幸蔵
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      麻布大学
    • Year and Date
      2009-04-02
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] マダニによるバベシア原虫媒介の分子基盤2009

    • Author(s)
      藤崎幸蔵
    • Organizer
      第21回日本薬学会微生物シンポジウム
    • Place of Presentation
      福山大学
    • Related Report
      2010 Final Research Report 2009 Annual Research Report 2009 Self-evaluation Report
  • [Presentation] マダニカテプシンL様システインプロテアーゼの性状解析2009

    • Author(s)
      山地佳代子, 辻尚利, 三好猛晴, 八田岳士, Abdul M.Alim, M.Anisuzzanman, 櫛引史郎, 藤崎幸蔵
    • Organizer
      第148回日本獣医学会学術集会
    • Place of Presentation
      鳥取大学
    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
  • [Presentation] 創薬開発のマダニ生物活性分子2009

    • Author(s)
      藤崎幸蔵
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      麻布大学
    • Related Report
      2010 Final Research Report 2009 Self-evaluation Report
  • [Presentation] マダニのオートファジー研究の展開:マダニとその媒介病原体の制圧に向けて2009

    • Author(s)
      白藤(梅宮)梨可, 松尾智英, 藤崎幸蔵
    • Organizer
      第147回日本獣医学会学術集会
    • Place of Presentation
      麻布大学
    • Related Report
      2010 Final Research Report
  • [Presentation] Strategy of a three host tick Haemaphysalis longicornis against starvation during the nonfeeding period2008

    • Author(s)
      Umemiya-Shirafuji R, Matsuo T, Boldbaatar D, Liao M, Tanaka T, Fujisaki K
    • Organizer
      6th International Conference on Tick and Tick-Borne Pathogens
    • Place of Presentation
      アルゼンチン
    • Related Report
      2010 Final Research Report
  • [Presentation] Babesial vector tick cysteine protease against Babesia sp. Parasites2008

    • Author(s)
      藤崎幸蔵, 辻尚利
    • Organizer
      16th Japanese-German Cooperative Symposium on Protozoan Diseases
    • Place of Presentation
      ドイツ
    • Related Report
      2010 Final Research Report
  • [Presentation] A tick cysteine protease, longipian, from the babesial parasite vector Haemaphysalis longicornis, with possible multifunctional roles during parasite transmission2008

    • Author(s)
      辻尚利, 藤崎幸蔵
    • Organizer
      US-Japan Cooperative Medical Science Program Parasitic Disease Panel
    • Place of Presentation
      アメリカ合衆国
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification and Molecular Characterization of a Vitellogenin Receptorfrom the Hard Tick Haemaphysalis longicornis2007

    • Author(s)
      Fujisaki K
    • Organizer
      21st World Association for the Advancement of Veterianry Parasitology
    • Place of Presentation
      Ghent, Belgium
    • Year and Date
      2007-08-19
    • Related Report
      2007 Annual Research Report
  • [Book] 図説獣医衛生動物学2009

    • Author(s)
      今井壮一、藤崎幸蔵、板垣匡、森田達志
    • Publisher
      講談社
    • Related Report
      2010 Final Research Report
  • [Book] 図説獣医衛生動物学2009

    • Author(s)
      今井壮一, 藤崎幸蔵, 板垣匡, 森田達志
    • Publisher
      講談社
    • Related Report
      2009 Self-evaluation Report
  • [Book] 最新家畜寄生虫病学2007

    • Author(s)
      藤崎幸蔵
    • Total Pages
      324
    • Publisher
      朝倉書店(東京)
    • Related Report
      2007 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://w3vet.agri.kagoshima-u.ac.jp/V-Infection/sinkoukansen/toppage.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] ダニのキチナーゼ2010

    • Inventor(s)
      藤崎幸蔵, ほか7名
    • Industrial Property Rights Holder
      明治製菓、藤崎幸蔵
    • Acquisition Date
      2010-02-05
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] ダニのキチナーゼ2010

    • Inventor(s)
      藤崎幸蔵, ほか7名
    • Industrial Property Rights Holder
      明治製菓、藤崎幸蔵
    • Acquisition Date
      2010-02-05
    • Related Report
      2009 Self-evaluation Report
  • [Patent(Industrial Property Rights)] ダニのキチナーゼ2010

    • Inventor(s)
      藤崎幸蔵, ほか7名
    • Industrial Property Rights Holder
      明治製菓・藤崎幸蔵
    • Acquisition Date
      2010-02-05
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] マダニのLKRとSDH2008

    • Inventor(s)
      藤崎幸蔵
    • Industrial Property Rights Holder
      鹿児島大学
    • Industrial Property Number
      2008-130019
    • Filing Date
      2008-05-16
    • Related Report
      2008 Annual Research Report

URL: 

Published: 2007-04-01   Modified: 2016-04-21  

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