Chemical Biology in internalization of membrane-permeable peptides
| Project/Area Number |
19209004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Drug development chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
FUTAKI Shiroh Kyoto University, 化学研究所, 教授 (50199402)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASE Ikuhiko 京都大学, 化学研究所, 助教 (40432322)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥51,350,000 (Direct Cost: ¥39,500,000、Indirect Cost: ¥11,850,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2008: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2007: ¥36,920,000 (Direct Cost: ¥28,400,000、Indirect Cost: ¥8,520,000)
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| Keywords | アルギニンペプチド / 膜透過ペプチド / マクロピノサイトーシス / 受容体 / 細胞内移行 / 細胞膜 / 光反応性架橋団 / 薬物送達 / アルギニン / 細胞内情報伝達 / アクチン重合化 |
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| Research Abstract |
Involvement of macropinocytosis to the internalization of arginine-rich membrane-permeable peptides has been suggested. In this study, using an R12 peptide bearing diazirine-based photo-reactive crosslinkers, a potential receptor to induce macropinocytosis by the interaction with R12 was identified. It was also elucidated that internalization efficiency of arginine-rich peptides are greatly affected by the peptide concentration, presence of serum, addition of hydrophobic segments and so on.
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Report
(4 results)
Research Products
(28 results)
