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Development of a novel detection method for abnormal prion protein

Research Project

Project/Area Number 19209020
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionNagasaki University

Principal Investigator

KAI Masaaki  Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (00160953)

Co-Investigator(Kenkyū-buntansha) 柴田 孝之  長崎大学, 大学院・医歯薬学総合研究科, 助教 (10448491)
椛島 力  長崎大学, 大学院・医歯薬学総合研究科, 准教授 (20274673)
Co-Investigator(Renkei-kenkyūsha) KABASHIMA Tsutomu  長崎大学, 大学院・医歯薬学総合研究科, 准教授 (20274673)
SHIBATA takayuki  長崎大学, 大学院・医歯薬学総合研究科, 助教 (10448491)
Project Period (FY) 2007 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000)
Fiscal Year 2010: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2009: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2008: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2007: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Keywordsプリオン / アプタマー / 感染症 / 核酸 / タンパク質 / 発光検出 / マイクロアレイ / 物理化学的性質 / マイクロアレー / 蛋白質
Research Abstract

The aim of this research is to develop the novel detection method for the abnormal prion protein, which causes the prion disease. The recombinant normal prion protein was expressed in E. coli, and purified with affinity chromatography. The denatured prion protein, which indicates resistance against protease digestion, was prepared by the treatment of the normal prion protein with cupric ions. In this project, we developed highly sensitive and specific detection methods for the prion proteins on a solid phase membrane using nucleic aptamer and chemiluminescent reagents.

Report

(6 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report   Self-evaluation Report ( PDF )
  • 2008 Annual Research Report
  • 2007 Annual Research Report
  • Research Products

    (103 results)

All 2011 2010 2009 2008 2007 Other

All Journal Article (40 results) (of which Peer Reviewed: 40 results) Presentation (43 results) Book (2 results) Remarks (6 results) Patent(Industrial Property Rights) (12 results) (of which Overseas: 2 results)

  • [Journal Article] Aptamer-barcode based immunoassay for the instantaneous derivatization chemiluminescence detection of IgE coupled to magnetic beads.2011

    • Author(s)
      Peng Q, Cao Z, Lau C, Kai M, Lu J.
    • Journal Title

      Analyst 136

      Pages: 140-147

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aptamer-barcode based immunoassay for the instantaneous derivatization chemiluminescence detection of IgE coupled to magnetic beads.2011

    • Author(s)
      Qianwen Peng
    • Journal Title

      Analyst

      Volume: 136 Pages: 140-147

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alginic acid-based macromolecular chemiluminescent probe for universal protein assay on a solid-phase membrane.2010

    • Author(s)
      Krawczyk T, Kondo M, Azam MG, Zhang H, Shibata T, Kai M.
    • Journal Title

      Analyst 135

      Pages: 2894-2900

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction.2010

    • Author(s)
      Yu Z, Kabashima T, Tang C, Shibata T, Kitazato K, Kobayashi N, Lee MK, Kai M.
    • Journal Title

      Anal.Biochem. 397

      Pages: 197-201

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] G-rich sequence-functionalized polystyrene microsphere-based instantaneous derivatization for the chemiluminescent amplified detection of DNA.2010

    • Author(s)
      Xin L, Cao Z, Lau C, Kai M, Lu J.
    • Journal Title

      Luminescence 25

      Pages: 336-342

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aptamer-mediated chemiluminescence detection of prion protein on a membrane using trimethoxyphenylglyoxal.2010

    • Author(s)
      Hossain MT, Shibata T, Kabashima T, Kai M.
    • Journal Title

      Anal.Sci. 26

      Pages: 645-647

    • NAID

      10026783879

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of L-DOPA-induced cytotoxicity in rat adrenal pheochromocytoma cells : implication of oxidative stress-related kinases and cyclic AMP.2010

    • Author(s)
      Jin CM, Yang YJ, Huang HS, Kai M, Lee MK.
    • Journal Title

      Neuroscience 170

      Pages: 390-398

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel and specific fluorescence reaction for uracil.2010

    • Author(s)
      Shibata T, Kawasaki SY, Fujita JY, Kabashima T, Kai M.
    • Journal Title

      Anal.Chim.Acta 674

      Pages: 234-238

    • NAID

      120006984283

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alginic acid-based macromolecular chemiluminescent probe for universal protein assay on a solid-phase membrane.2010

    • Author(s)
      Tomasz Krawczyk
    • Journal Title

      Analyst

      Volume: 135 Pages: 2894-2900

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction.2010

    • Author(s)
      Zhiqiang Yu
    • Journal Title

      Anal.Biochem

      Volume: 397 Pages: 197-201

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] G-rich sequence-functionalized polystyrene microsphere-based instantane ous derivatization for the chemiluminescent amplified detection of DNA.2010

    • Author(s)
      Liang Xin
    • Journal Title

      Luminescence

      Volume: 25 Pages: 336-342

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aptamer-mediated chemiluminescence detection of prion protein on a membrane using trimethoxyphenylglyoxal.2010

    • Author(s)
      Md Towhid Hossain
    • Journal Title

      Anal.Sci

      Volume: 26 Pages: 645-647

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mechanisms of L-DOPA-induced cytotoxicity in rat adrenal pheochromocytoma cells : implication of oxidative stress-related kinases and cyclic AMP.2010

    • Author(s)
      Jin CM
    • Journal Title

      Neuroscience

      Volume: 170 Pages: 390-398

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel and specific fluorescence reaction for uracil.2010

    • Author(s)
      Takayuki Shibata
    • Journal Title

      Anal.Chim.Acta

      Volume: 674 Pages: 234-238

    • NAID

      120006984283

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Selective and facile assay of HIV protease activity by a novel fluorogenic reaction2010

    • Author(s)
      ZhiQiang Yu
    • Journal Title

      Anal.Biochem. 397

      Pages: 197-201

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer.2009

    • Author(s)
      Zhang H, Shibata T, Krawczyk T, Kabashima T, Lu J, Lee MK, Kai M.
    • Journal Title

      Talanta 79

      Pages: 700-705

    • NAID

      120006984124

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Label-free aptamer-based chemiluminescence detection of adenosine.2009

    • Author(s)
      Yan X, Cao Z, Kai M, Lu J.
    • Journal Title

      Talanta 79

      Pages: 383-387

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diimine ligand as a novel chemiluminescence enhancer of luminol-containing compounds.2009

    • Author(s)
      Smanmoo C, Yamasuji M, Sagawa T, Shibata T, Kabashima T, Yuan DQ, Fujita K, Kai M.
    • Journal Title

      Talanta 77

      Pages: 1761-1766

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemiluminescence platforms in immunoassay and DNA analyses.2009

    • Author(s)
      Fan A, Cao Z, Li H, Kai M, Lu J.
    • Journal Title

      Anal.Sci. 25

      Pages: 587-597

    • NAID

      10025211962

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer2009

    • Author(s)
      Huan Zhang, Takayuki Shibata, Tomasz Krawczyk, Tsutomu Kabashima, Jianzhong Lu, Myung K. Leec, Masaaki Kai
    • Journal Title

      Talanta 79(3)

      Pages: 700-705

    • NAID

      120006984124

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer2009

    • Author(s)
      Huan Zhang
    • Journal Title

      Talanta 79

      Pages: 700-705

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Label-free aptamer-based chemiluminescence detection of adenosine2009

    • Author(s)
      Xiluan Yan
    • Journal Title

      Talanta 79

      Pages: 383-387

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diimine ligand as a novel chemiluminescence enhancer of luminol-containing compounds2009

    • Author(s)
      Smanmoo Chaivat
    • Journal Title

      Talanta 77

      Pages: 1761-1766

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemiluminescence platforms in immunoassay and DNA analyses2009

    • Author(s)
      Aiping Fan
    • Journal Title

      Analytical Sciences 25

      Pages: 587-597

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diimine ligand as a novel chemiluminescence enhancer of luminol-containing compounds2009

    • Author(s)
      C. Smanmoo
    • Journal Title

      Talanta 77

      Pages: 1761-1766

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent.2008

    • Author(s)
      Tonooka K, Kabashima T, Shibata T, Tang C, Yu Z, Kai M.
    • Journal Title

      Anal.Sci. 24

      Pages: 471-475

    • NAID

      10021099521

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of dopamine biosynthesis by 1-DOPA in PC12 cells : implications of L-DOPA influx and cyclic AMP.2008

    • Author(s)
      Jin CM, Yang YJ, Huang HS, Lim SC, Kai M, Lee MK.
    • Journal Title

      Eur.J.Pharmacol. 591

      Pages: 88-95

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A selective fluorescence reaction for peptides and chromatographic analysis.2008

    • Author(s)
      Kabashima T, Yu Z, Tang C, Nakagawa Y, Okumura K, Shibata T, Lu J, Kai M.
    • Journal Title

      Peptides 29

      Pages: 356-363

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescence detection of amino acids in the postcleavage conversions for manual sequencing of a peptide.2008

    • Author(s)
      Wainaina MN, Shibata T, Smanmoo C, Kabashima T, Kai M.
    • Journal Title

      Anal.Biochem. 374

      Pages: 423-425

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent2008

    • Author(s)
      K. Tonooka
    • Journal Title

      Anal. Sci. 24

      Pages: 471-475

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of dopamine biosynthesis by l-DOPA in PC12 cells : implications of L-DOPA influx and cyclic AMP2008

    • Author(s)
      CM. Jin
    • Journal Title

      Eur. J. Pharmacol. 591

      Pages: 88-95

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A selective fluorescence reaction for peptides and chromatographic analysis2008

    • Author(s)
      T. Kabashima
    • Journal Title

      Peptides 29

      Pages: 356-363

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescence detection of amino acids in the postcleavage conversions for manual sequencing of a peptide2008

    • Author(s)
      M. N. Wainaina
    • Journal Title

      Anal. Biochem. 374

      Pages: 423-425

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dextran-based polymeric chemiluminescent compounds for the sensitive optical imaging of a cytochrome p450 protein on a solid-phase membrane.2007

    • Author(s)
      Zhang H, Smanmoo C, Kabashima T, Lu J, Kai M.
    • Journal Title

      Angew.Chem.Int.Ed.Engl. 46

      Pages: 8226-8229

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Facile determination of DNA-binding nuclear factor-kappaB by chemiluminescence detection.2007

    • Author(s)
      Tonooka K, Kabashima T, Yamasuji M, Kai M.
    • Journal Title

      Anal.Biochem. 364

      Pages: 30-36

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cerium complexes of cyclodextrin dimers as efficient catalysts for luminol chemiluminescence reactions.2007

    • Author(s)
      Yuan DQ, Lu J, Atsumi M, Yan JM, Kai M, Fujita K.
    • Journal Title

      Org.Biomol.Chem. 5

      Pages: 2932-2939

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dextran-based polymeric chemiluminescent compounds for the sensitive optical imaging of a cytochrome P450 protein on a solid-phase membrane2007

    • Author(s)
      Huan Zhang, Chaivat Smanmoo, Tsutomu Kabashima, Jianzhong Lu, Masaaki Kai
    • Journal Title

      Angew.Chem.Int.Ed. 46(43)

      Pages: 8226-8229

    • Related Report
      2009 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Dextran-based polymeric chemiluminescent compounds for the sensitive optical imaging of a cytochrome p450 protein on a solid-phase membrane2007

    • Author(s)
      H. Zhang
    • Journal Title

      Angew. Chem. Int. Ed. 46

      Pages: 8226-8229

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Facile detemination of DNA-binding nuclear factor-κB by chemiluminescence detection2007

    • Author(s)
      K. Tonooka
    • Journal Title

      Anal. Biochem. 364

      Pages: 30-36

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cerium complexes of cyclodextrin dimmmers as efficient for luminol chemiluminescence reactions2007

    • Author(s)
      D-Q. Yuan
    • Journal Title

      Org. Biomol. Chem. 5

      Pages: 2932-2939

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] 3,4-dihydroxyphenylacetic acidを用いたペプチドの蛍光検出法2011

    • Author(s)
      Hasina Yasmin
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡市
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] ペプチド選択的な蛍光反応のカスパーゼ活性測定への応用2011

    • Author(s)
      Rahman Md.Shafikur
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡市
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウラシル特異的蛍光誘導体化反応を用いた尿中ウラシルのハイスループット定量法2011

    • Author(s)
      藤田順也
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡市
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] TMPG反応を用いた膜上テロメアDNAの高感度検出法の開発2010

    • Author(s)
      山筋睦美
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 特異的蛍光誘導体化反応による尿中ウラシルの定量2010

    • Author(s)
      藤田順也
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Assay method for caspase activity with a selective fluorescence reaction for peptides2010

    • Author(s)
      唐辰虹
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Alkaline phosphatase-labelled chemiluminescent probe for sensitive immunoassay of proteins on a solid-phase membrane2010

    • Author(s)
      Md.Golam Azam
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] ルミノールを基本骨格とする新規化学発光ラベル化剤2010

    • Author(s)
      吉村裕紀
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] HIVプロテアーゼを認識するアプタマーの探索2010

    • Author(s)
      河井健真
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 人工siRNAによるHIVプロテアーゼの発現阻害2010

    • Author(s)
      長谷康志
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Aptamer-mediated chemiluminescence detection of prion protein using trimethoxyphenylglyoxal on membrane2010

    • Author(s)
      Hossain Md.Towhid
    • Organizer
      第23回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      宮城県宮城郡松島町
    • Year and Date
      2010-07-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新規ペプチド蛍光誘導体化反応を基にしたウイルス識別法の開発2010

    • Author(s)
      椛島力
    • Organizer
      第23回バイオメディカル分析科学シンポジウム
    • Place of Presentation
      宮城県宮城郡松島町
    • Year and Date
      2010-07-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高感度化学発光イメージングのための酵素的デキストランプローブ2010

    • Author(s)
      Golam Azam, et al
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山市
    • Related Report
      2010 Final Research Report
  • [Presentation] プリオンタンパク質の溶解性における銅イオンの影響2010

    • Author(s)
      室田紗由美, et al
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Alkaline phosphatase-labelled chemiluminescent probe for sensitive immunoassay of proteins on a solid-Phase membrane2010

    • Author(s)
      Golam Azam, et al
    • Organizer
      第27回日本薬学会九州支部大会
    • Place of Presentation
      長崎市
    • Related Report
      2010 Final Research Report
  • [Presentation] Aptamer-mediated chemiluminescence detection of prion protein using trimethoxyphenyl glyoxal on membrane2010

    • Author(s)
      Hossain Md.Towhid, et al
    • Organizer
      第23回バイオメデイカル分析科学シンポジウム
    • Place of Presentation
      宮城県宮城郡松島町
    • Related Report
      2010 Final Research Report
  • [Presentation] A fluorogenic-based assay for facile discrimination of mutant HIV-1 proteases2010

    • Author(s)
      Yu ZhiQiang
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Evaluation on siRNA-induced inhibition of HIV-1 protease in mammalian cells2010

    • Author(s)
      Zhang Huan
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dextran-based enzymatic probes for sensitive imaging of chemiluminescence2010

    • Author(s)
      Md.Golam Azam
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山市
    • Related Report
      2009 Annual Research Report
  • [Presentation] New tools for the research on HIV and prion diseases2009

    • Author(s)
      Masaaki Kai
    • Organizer
      The second Asia symposium on pharmaceutical sciences in Nagasaki
    • Place of Presentation
      長崎市
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Development of simple method for DNA aptamer against protein2009

    • Author(s)
      Hossain Md.Towhid, et al
    • Organizer
      The second Asia symposium on pharmaceutical sciences in Nagasaki
    • Place of Presentation
      長崎市
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] New tools for the research on HIV and prion diseases2009

    • Author(s)
      Masaaki Kai
    • Organizer
      The second Asia symposium on pharmaceutical sciences in Nagasaki
    • Place of Presentation
      Nagasaki
    • Related Report
      2009 Self-evaluation Report
  • [Presentation] 修飾塩基を持つsiRNAの設計と干渉効果の評価(1)2009

    • Author(s)
      塩崎真里
    • Organizer
      第26回日本薬学会九州支部大会
    • Place of Presentation
      福岡市
    • Related Report
      2009 Annual Research Report
  • [Presentation] Polysaccaride-based chemiluminescent probe for proteins detection on a membrane2009

    • Author(s)
      Tomasz Krawczyk
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Sequential analysis of amino acids in peptides with fluorogenic Edman reagent, 4-(1'-cyanoisoindolyl)phenylisothiocyanate2009

    • Author(s)
      Moses N. Wainaina
    • Organizer
      日本薬学会第129年会
    • Place of Presentation
      京都市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Chemiluminescence platforms in immunoassay and DNA analyses2009

    • Author(s)
      Jianzhong Lu
    • Organizer
      The second Asia symposium on pharmaceutical sciences in Nagasaki
    • Place of Presentation
      長崎市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Alginic acid-based macromolecular probe for chemiluminescent detection of protein2009

    • Author(s)
      Tomasz Krawczyk
    • Organizer
      The second Asia symposium on pharmaceutical sciences in Nagasaki
    • Place of Presentation
      長崎市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of sequence determination method for peptides with fluorogenic Edman reagent, 4-(1'-cyanoisoindolyl)phenylisothiocyanate2009

    • Author(s)
      Moses N. Wainaina
    • Organizer
      The second Asia symposium on pharmaceutical sciences in Nagasaki
    • Place of Presentation
      長崎市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Novel fluorometric assay for identification of mutant HIV proteases with HPLC2009

    • Author(s)
      Zhiqiang Yu
    • Organizer
      The second Asia symposium on pharmaceutical sciences in Nagasaki
    • Place of Presentation
      長崎市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Application of DNA detectable chemiluminogenic reagent (TMPG) to telomerase assay2008

    • Author(s)
      Tsutomu Kabashima, et al
    • Organizer
      Pure and applied chemistry international conference 2008
    • Place of Presentation
      バンコク(タイ)
    • Related Report
      2010 Final Research Report
  • [Presentation] Selection of DNA aptamer against cytochrome P4502008

    • Author(s)
      Hossain Md. Towhid
    • Organizer
      第25回日本薬学会九州支部大会
    • Place of Presentation
      延岡市
    • Related Report
      2008 Annual Research Report
  • [Presentation] A fluorometric HPLC method for the assay of HIV protease activity2008

    • Author(s)
      Yu Zhiqiang
    • Organizer
      日本分析化学会第57年会
    • Place of Presentation
      福岡市
    • Related Report
      2008 Annual Research Report
  • [Presentation] ピリミジン塩基を識別できる新規蛍光反応の開発と反応機構の解明2008

    • Author(s)
      川崎 慎也
    • Organizer
      日本分析化学会第57年会
    • Place of Presentation
      福岡市
    • Related Report
      2008 Annual Research Report
  • [Presentation] Preparation of super-sensitive luminescent probe for detection genes and proteins on microchips2008

    • Author(s)
      C. Smanmoo
    • Organizer
      Pure and applied chemistry international conference 2008
    • Place of Presentation
      バンコク(タイ)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Application of DNA-detectable chemiluminogenic reagent(TMPG) to telomeraseassay2008

    • Author(s)
      T. Kabashima
    • Organizer
      Pure and applied chemistry international conference 2008
    • Place of Presentation
      バンコク(タイ)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression of mouse prion protein in E.coli to develop aptamer2007

    • Author(s)
      Kenji Koba, et al
    • Organizer
      International conference in structural biology
    • Place of Presentation
      香港(中国)
    • Related Report
      2010 Final Research Report 2007 Annual Research Report
  • [Presentation] Development of novel fluorescence-detection method for uracil and cytosine2007

    • Author(s)
      S. Kawasaki
    • Organizer
      International conference in structural biology
    • Place of Presentation
      香港(中国)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Sensitive fluorescence-detection method of amino acids for protein sequencing2007

    • Author(s)
      T. Shibata
    • Organizer
      International conference in structural biology
    • Place of Presentation
      香港(中国)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Expression of carbohydrate-response element-binding protein fused with GFP in two cell lines2007

    • Author(s)
      CH. Tang
    • Organizer
      International conference in structural biology
    • Place of Presentation
      香港(中国)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Chemiluminescence detection of proteins on membranes employing DNA probe and TMPG reagent2007

    • Author(s)
      M. Yamasuji
    • Organizer
      International conference in structural biology
    • Place of Presentation
      香港(中国)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Highly selective fluorometric assay for HIV-1 protease activity2007

    • Author(s)
      ZQ. Yu
    • Organizer
      International conference in structural biology
    • Place of Presentation
      香港(中国)
    • Related Report
      2007 Annual Research Report
  • [Presentation] Evaluation of dextran-based polymeric chemiluminescent compounds for the direct detection of proteins on a membrane2007

    • Author(s)
      H. Zhang
    • Organizer
      International conference in structural biology
    • Place of Presentation
      香港(中国)
    • Related Report
      2007 Annual Research Report
  • [Presentation] A simple bicyclic cytosine analogue that stabilizes DNA duplexes2007

    • Author(s)
      T. Shibata
    • Organizer
      7th International meeting on recognition studies in nucleic acids
    • Place of Presentation
      シェフィールド(イギリス)
    • Related Report
      2007 Annual Research Report
  • [Book] 薬学物理化学[第5版]2010

    • Author(s)
      小野行雄編集
    • Publisher
      廣川書店
    • Related Report
      2010 Final Research Report
  • [Book] 薬学物理化学[第5版](第5章 電解質溶液pp79-98)2010

    • Author(s)
      甲斐雅亮、椛島力(小野行雄編集)
    • Total Pages
      379
    • Publisher
      廣川書店
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/function/index-j.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/function/index-j.html

    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/function/index-j.html

    • Related Report
      2009 Self-evaluation Report
  • [Remarks]

    • URL

      http://www.ph.nagasaki-u.acjp/lab/function/index-j.html

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/function/index-j.html

    • Related Report
      2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.ph.nagasaki-u.ac.jp/lab/function/index-j.html

    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] ペプチドの検出方法2011

    • Inventor(s)
      甲斐雅亮、椛島力、柴田孝之
    • Industrial Property Rights Holder
      長崎大学
    • Industrial Property Number
      2011-045491
    • Filing Date
      2011-03-02
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 2環状シトシン誘導体含有人工二本鎖核酸2011

    • Inventor(s)
      甲斐雅亮、柴田孝之、椛島力
    • Industrial Property Rights Holder
      長崎大学
    • Industrial Property Number
      2011-045485
    • Filing Date
      2011-03-02
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] ペプチドの検出方法2011

    • Inventor(s)
      甲斐雅亮, 椛島力, 柴田孝之
    • Industrial Property Rights Holder
      長崎大学
    • Industrial Property Number
      2011-045491
    • Filing Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 2環状シトシン誘導体含有人工二本鎖核酸2011

    • Inventor(s)
      甲斐雅亮, 柴田孝之, 椛島力
    • Industrial Property Rights Holder
      長崎大学
    • Industrial Property Number
      2011-045485
    • Filing Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] ウイルスの識別方法2010

    • Inventor(s)
      甲斐雅亮、椛島力
    • Industrial Property Rights Holder
      長崎大学
    • Industrial Property Number
      2010-047640
    • Filing Date
      2010-03-04
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] ウラシル特異的な蛍光検出反応及びジヒドロピリミジンデヒドロゲナーゼ欠損症の検査法2010

    • Inventor(s)
      甲斐雅亮、柴田孝之
    • Industrial Property Rights Holder
      長崎大学
    • Industrial Property Number
      2010-044610
    • Filing Date
      2010-03-01
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Patent(Industrial Property Rights)] ウラシル特異的な蛍光検出反応及びジヒドロピリミジンデヒドロゲナーゼ欠損症の検査法2010

    • Inventor(s)
      甲斐雅亮, 柴田孝之
    • Industrial Property Rights Holder
      長崎大学
    • Industrial Property Number
      2010-044610
    • Filing Date
      2010-03-01
    • Related Report
      2009 Self-evaluation Report
  • [Patent(Industrial Property Rights)] ウイルスの識別方法2010

    • Inventor(s)
      甲斐雅亮, 椛島力
    • Industrial Property Rights Holder
      長崎大学
    • Filing Date
      2010-03-04
    • Related Report
      2009 Self-evaluation Report
  • [Patent(Industrial Property Rights)] ウイルスの識別方法2010

    • Inventor(s)
      甲斐雅亮、椛島力
    • Industrial Property Rights Holder
      長崎大学
    • Filing Date
      2010-03-04
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] Luminescent polymer and use there of in bioassay2008

    • Inventor(s)
      甲斐雅亮
    • Industrial Property Rights Holder
      長崎大学
    • Acquisition Date
      2008-10-03
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] LUMINESCENT POLYMER AND USE THEREOF IN BIOASSAY2008

    • Inventor(s)
      甲斐雅亮
    • Industrial Property Rights Holder
      長崎大学
    • Filing Date
      2008-10-03
    • Related Report
      2009 Self-evaluation Report
    • Overseas
  • [Patent(Industrial Property Rights)] Luminescent polymer and use thereof in bioassay2008

    • Inventor(s)
      Masaaki Kai
    • Industrial Property Rights Holder
      M. Kai, Daiichi Pure Chemicals
    • Acquisition Date
      2008-10-03
    • Related Report
      2008 Annual Research Report
    • Overseas

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Published: 2007-04-01   Modified: 2016-04-21  

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