| Budget Amount *help |
¥49,400,000 (Direct Cost: ¥38,000,000、Indirect Cost: ¥11,400,000)
Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2009: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2007: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
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| Research Abstract |
In order to clarify the role of AIRE gene in resist against Candida infection, we used Aire knockout mice and studied the expression of AIRE gene and protein in human peripheral immune cells. Interestingly, AIRE gene was expressed distinctively in CD4+ T cells, B cells and dendritic cells. Although AIRE gene was expressed in CD4+ T cells, AIRE protein was absent in those cells. We found that hsa-miR-anti-AIRE microRNA (tentative name) was highly expressed in CD4+ T cells, but lower in EB virus transformed B (EBV-B) cells expressing both AIRE gene and protein, indicating the microRNA may play an important role in regulating the translation of AIRE gene. We also found that both 58kDa and 45kDa AIRE related protein was expressed in dendritic cells, while EBV-B cells only expressed only 45 kDa AIRE protein, indicating multiple AIRE protein function dependent on cells expressing AIRE protein.
Experimentally, Aire knockout mice were susceptible to intravenous candida infection as worse as Rag-1 KO mice, whereas T cell deficient nu/nu mice or B cell deficient mu/mu mice could resist against candida infection, suggesting that either intact T cells or B cells are sufficient to protect against candida infection.
Whether any interactive protein or gene associate with AIRE protein remain uncertain and exact functions of AIRE gene require further study.
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