| Project/Area Number |
19209045
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nihon University |
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Principal Investigator |
TAKAYAMA Tadatoshi Nihon University, 医学部, 教授 (30280944)
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| Co-Investigator(Kenkyū-buntansha) |
MAKUUCHI Masatoshi 東京大学, 医学部, 名誉教授 (60114641)
INOUE Kazuto 日本大学, 医学部, 准教授 (00372996)
NAKAYAMA Hisashi 日本大学, 医学部, 講師 (00287632)
MIKI Kenji 日本大学, 医学部, 兼任講師 (20386014)
YAMAZAKI Shintaro 日本大学, 医学部, 助手 (20409014)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥48,360,000 (Direct Cost: ¥37,200,000、Indirect Cost: ¥11,160,000)
Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2008: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2007: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
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| Keywords | 膵島移植 / 膵β細胞 / PDX-1 / インスリン / 肝再生 / 骨髄幹細胞 / 分化誘導 / 骨髄キメラ / 特異的免疫寛容 |
|---|
| Research Abstract |
The development of effective immunosuppressants has dramatically improved both graft and patient survival rates in organ transplantation, and has contributed to advances in medical transplantation during the past few years. Inducing donor-specific tolerance (DST) is widely believed to be the most effective solution to these problems. Chimerism is viewed as an attractive approach to inducing DST, and many studies of this phenomenon have been published. A mixed chimera can be created by transplanting bone marrow (BM) from histoincompatible donor mice to lethally-irradiated recipient mice, together with BM that is histocompatible with the recipient mice, so that the recipient haematopoietic cells can be partially replaced by donor-compatible cells. Compared to an allochimera, a mixed chimera is more stable and less susceptible to the lethal BM aplasia associated with BM graft rejection. We have focused on using splenocytes as a source of cells for establishing tolerance, in view of their availability for use in clinical practice. Splenocytes are an attractive source of cells with which to develop chimerism because they are a rich source of lymphocytes. In the current study, we verified the hypothesis that splenocytes containing abundant immunocytes are effective for maintaining chimerism and improving long-term graft survival in mice.
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