| Project/Area Number |
19209047
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KAWAGUCHI NANAKO (KAWAGUCHI Nanako) (2009-2010) Tokyo Women's Medical University, 神経研究所 遺伝子疾患治療研究部, 科研費研究員 (10200700)
黒澤 博身 (2007-2008) Tokyo Women's Medical University, 医学部, 教授 (50075511)
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| Co-Investigator(Kenkyū-buntansha) |
SAITOH Satoshi 東京女子医科大学, 医学部, 講師 (60246551)
MATSUMURA Goki 東京女子医科大学, 医学部, 助教 (20297469)
佐藤 志樹 東京女子医科大学, 医学部, 助教 (40235403)
川口 奈奈子 東京女子医科大学, 国際統合医科学インスティチュート, 講師 (10200700)
坂本 貴彦 東京女子医科大学, 医学部, 助教 (80246553)
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| Project Period (FY) |
2007 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥51,740,000 (Direct Cost: ¥39,800,000、Indirect Cost: ¥11,940,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2007: ¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
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| Keywords | 心臓幹細胞c-kit / GATA4 / 3次元培養 / cardiac / stem cell / 再生医療 / 心臓 / 培養 / characterization / IGF-1 / 心筋 / 幹細胞 / 分化 / 再生 / 三次元培養 / 成体心臓由来幹細胞 / 組織幹細胞 / 心筋細胞 / ナノーテクノロジー / TGF-βスーパーファミリー / 心筋梗塞 / c-kit / スキャッフォールド / ティッシュエンジニアリング / 幹 細胞 / 遺伝子解析 / 成長因子 |
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| Research Abstract |
The aim of this study is to establish 3D culture for transplantation to the infarcted heart. Cardiac stem cells obtained as c-kit positive cells from adult rat hearts were used as a cell source. Even if we use the same method to obtain c-kit positive cells, different genes expression pattern was observed after the long-term culture. Among the various cardiac stem cells (CSCs), GATA4 high-expressing CSCs showed more potential to differentiate into cardiac myocytes than GATA4 low-expressing cells in vitro. Furthermore, these GATA4 high CSCs had more survival effect on the co-cultured cardiacmyocytes derived from adult rat heart than GATA4 low-expressing CSCs. Therefore,GATA4 high CSCs are the appropriate cell source for regenerative medicine for heart failure. We also showed that TGF-βsuperfamily associates with myogenesis.
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