Surveillance of human African trypanosomiases transmitted from domestic and wild animals and detection of virulence factors

• Project/Area Number: 19255015
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: SUGIMOTO Chihiro Hokkaido University, 人獣共通感染症リサーチセンター, 教授 (90231373)
• Co-Investigator(Kenkyū-buntansha): KAJINO Kiichi 北海道大学, 人獣共通感染症リサーチセンター, 准教授 (80322147) ; NAKAMURA Ichirou 北海道大学, 人獣共通感染症リサーチセンター, 講師 (20374241) ; INOUE Noboru 帯広畜産大学, 原虫病研究センター, 准教授 (10271751) ; 渡邊 純一 (渡辺 純一) 東京大学, 医科学研究所, 助教 (20201189)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥33,930,000 (Direct Cost: ¥26,100,000、Indirect Cost: ¥7,830,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2007: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
• Keywords: アフリカ / トリパノソーマ / ツェツェバエ / 病原性 / ゲノム

Research Abstract

Molecular epidemiological surveillance of human and animal had been conducted in Zambia and Sudan and human cases were confirmed in the Eastern Province in Zambia. Tsetse flies (Glossina morsitans) were infected with human-infective Trypanosoma brucei rhodesiense at high infection ratio, and warthog and kudu were supposed to serve as natural reservoirs. In Sudan, high infection rate with T. evansi was observed in camels.

Research Products

• [Journal Article] Rapid discrimination and quantification of Theileria orientalis types using ribosomal DNA internal transcribed spacers (2011)
  • Author(s): Kamau J., 他
  • Journal Title: Infect Genet Evol. 11 Pages: 407-414
  • Peer Reviewed
• [Journal Article] Emergence of new types of Theileria orientalis in Australian cattle and possible cause of theileriosis outbreaks (2011)
  • Author(s): Kamau J., 他
  • Journal Title: Parasit Vectors 21 Pages: 22-22
  • Peer Reviewed
• [Journal Article] Molecular epidemiology of camel trypanosomiasis based on ITS1 rDNA and RoTat 1.2 VSG gene in the Sudan. (2011)
  • Author(s): Salim B., 他
  • Journal Title: Parasit Vectors. 4 Pages: 31-31
  • Peer Reviewed
• [Journal Article] Genotypic diversity of Theileria orientalis detected from cattle grazing in Kumamoto and Okinawa Prefectures of Japan. (2011)
  • Author(s): Yokoyama N., 他
  • Journal Title: J.Vet.Med.Sci. 73 Pages: 305-312
  • Peer Reviewed
• [Journal Article] Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP) (2010)
  • Author(s): Thekisoe O.M., 他
  • Journal Title: Am.J.Trop.Med.Hyg. 82 Pages: 855-860
  • Peer Reviewed
• [Journal Article] Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene within Theileria equi from horses in Sudan (2010)
  • Author(s): Salim B., 他
  • Journal Title: Parasitol Res. 106 Pages: 493-498
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification (LAMP) assays for detection of Theileria parva infections targeting the PIM and p150 genes (2010)
  • Author(s): Thekisoe O.M., 他
  • Journal Title: Int.J.Parasitol. 40 Pages: 55-61
  • Peer Reviewed
• [Journal Article] Development of loop-mediated isothermal amplification (LAMP) assays for rapid detection of Ehrlichia ruminantium (2010)
  • Author(s): Nakao R., 他
  • Journal Title: BMC Microbiol. 10 Pages: 296-296
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification (LAMP) assays fordetection of Theileria parva infections targeting the PIM and p150 genes. (2010)
  • Author(s): Thekisoe O.M.
  • Journal Title: Int J Parasitol. Volume: 40 Pages: 55-61
  • Peer Reviewed
• [Journal Article] Detection of Trypanosoma cruzi and T.rangeli infections from Rhodnius pallescens bugs by loop-mediated isothermal amplification (LAMP). (2010)
  • Author(s): T Thekisoe O.M.
  • Journal Title: Am J Trop Med Hyg. Volume: 82 Pages: 855-860
  • Peer Reviewed
• [Journal Article] Genotypic diversity of Theileria orientalis detected from cattle grazing in Kumamoto and Okinawa Prefectures of Japan. (2010)
  • Author(s): Yokoyama, N.
  • Journal Title: J.Vet.Med.Sci. Volume: 73 Pages: 305-312
  • Peer Reviewed
• [Journal Article] Stability of Loop-Mediated Isothermal Amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates (2009)
  • Author(s): Thekisoe O.M., 他
  • Journal Title: J.Vet.Med.Sci. 71 Pages: 525-527
  • Peer Reviewed
• [Journal Article] A field study to estimate the prevalence of bovine African Trypanosomosis in Butaleja District, Uganda (2009)
  • Author(s): Jing Z., 他
  • Journal Title: J.Vet.Med.Sci. 71 Pages: 525-527
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of a T24-like protein in Echinococcus multilocularis (2009)
  • Author(s): Dang Z., 他
  • Journal Title: Mol.Biochem.Parasitol 168 Pages: 117-119
  • Peer Reviewed
• [Journal Article] Preliminary studies on the effects of orally-administered transforming growth factor-beta on protozoan diseases in mice (2009)
  • Author(s): Namangala B., Inoue N., Sugimoto C.
  • Journal Title: Jpn.J.Vet.Res. 57 Pages: 101-108
  • NAID: 120006390421
  • Peer Reviewed
• [Journal Article] Characterization of a leucine aminopeptidase of Babesia gibsoni. (2009)
  • Author(s): Jia H., 他
  • Journal Title: Parasitology 136 Pages: 945-952
  • Peer Reviewed
• [Journal Article] Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis (2009)
  • Author(s): Dang Z., 他
  • Journal Title: Vaccine 27 Pages: 7339-7345
  • Peer Reviewed
• [Journal Article] Characterization of a concealed antigen Hq05 from the hard tick Haemaphysalis qinghaiensis and its effect as a vaccine against tick infestation in sheep (2009)
  • Author(s): Gao J., 他
  • Journal Title: Vaccine 56 Pages: 171-180
  • Peer Reviewed
• [Journal Article] A field study to estimate the prevalence of bovine African Trypanosomosis in Butaleja District, Uganda (2009)
  • Author(s): Jing Z, Magona JW, Sakurai T, Thekisoe OM, Otim CP, Sugimoto C, Inoue N.
  • Journal Title: J Vet Med Sci. 71(4) Pages: 525-527
  • NAID: 120006390412
• [Journal Article] Stability of Loop-Mediated Isothermal Amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates (2009)
  • Author(s): Thekisoe OM, Bazie RS, Coronel-Servian AM, Sugimoto C, Kawazu S, Inoue N.
  • Journal Title: J Vet Med Sci. 71(4) Pages: 471-475
  • NAID: 120006390413
• [Journal Article] Preliminary studies on the effects of orally-administered Transforming Growth Factor-beta on protozoan diseases in mice (2009)
  • Author(s): Namangala B Inoue N. Sugimoto C.
  • Journal Title: Jpn J Vet Res. 57(2) Pages: 101-108
  • NAID: 120006390421
• [Journal Article] Stability of Loop-Mediated Isothermal Amplification(LAMP)reagents and its amplification efficiency on crude trypanosome DNA templates. (2009)
  • Author(s): Thekisoe O.M., Bazie R.S., Coronel-Servian A.M., Sugimoto C., Kawazu S. Inoue N.
  • Journal Title: Journal of Veterinary Medical Science. 71 Pages: 471-475
  • NAID: 120006390413
  • Peer Reviewed
• [Journal Article] A field study to estimate the prevalence of bovine African Trypanosomosis in Butaleja District, Uganda. (2009)
  • Author(s): Jing Z., Magona J.W., Sakurai T., Thekisoe O.M., Otim C.P., Sugimoto C., Inoue N.
  • Journal Title: Journal of Veterinary Medical Science. 71 Pages: 525-527
  • NAID: 120006390412
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of a T24-like protein in Echinococcus multiloeularis. (2009)
  • Author(s): Dang Z., Watanabe J., Kajino K., Oku Y., Matsumoto J., Yagi K., Kouguchi H., Sugimoto C.
  • Journal Title: Molecular Biochemical Parasitology. 168 Pages: 117-119
  • Peer Reviewed
• [Journal Article] Preliminary studies on the effects of orally-administered transforming growth factor-beta on protozoan diseases in mice. 200. (2009)
  • Author(s): Namangala B., Inoue N., Sugimoto C.
  • Journal Title: Japanese Jourmal of Veterinary Research. 57 Pages: 101-108
  • Peer Reviewed
• [Journal Article] Characterization of a leucine aminopeptidase of Babesia gibsoni. (2009)
  • Author(s): Jia H., Terkawi M.A., Aboge G.O., Goo Y.K., Luo Y., Li Y., Yamagishi J., Nishikawa Y., Igarashi I., Sugimoto C., Fujisaki K., Xuan X.
  • Journal Title: Parasitology 136 Pages: 945-952
  • Peer Reviewed
• [Journal Article] Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis. (2009)
  • Author(s): Dang Z., Yagi K., Kouguchi H., Kajino K., Watanabe J., Matsumoto J., Nakao R., Wakaguri H., Toyoda A., Sugimoto C.
  • Journal Title: Vaccine 27 Pages: 7339-7345
  • NAID: 120001821839
  • Peer Reviewed
• [Journal Article] Characterization of a concealed antigen Hq05 from the hard tick Haemaphysalis qinghaiensis and its effect as a vaccine against tick infestation in sheep. (2009)
  • Author(s): Gao J., Luo J., Fan R., Schulte-Spechtel U.C., Fingerle V., Guan G., Zhao H., Li Y., Ren Q., Ma M., Liu Z., Liu A., Dang Z., Sugimoto C., Yin H.
  • Journal Title: Vaccine 27 Pages: 483-490
  • Peer Reviewed
• [Journal Article] A perspective on Theileria equi infections in donkeys. Review (2009)
  • Author(s): Kumar S, Kumar R, Sugimoto C.
  • Journal Title: Japanese Journal of Veterinary Research. 56 Pages: 171-180
  • Peer Reviewed
• [Journal Article] Characterization of a concealed antigen Hq05 from the hard tick Haemaphysalis qinghaiensis and its effect as a vaccine against tick infestation in sheep. (2009)
  • Author(s): Gao J, Luo J, Fan R, Schulte-Spechtel UC, Fingerle V, Guan G, Zhao H, Li Y, Ren Q, Ma M, Liu Z, Liu A, Dand Z, Sugimoto C, Yin H.
  • Journal Title: Vaccine 14 Pages: 483-490
  • Peer Reviewed
• [Journal Article] Effect of CD4(+)CD25(+) T Cell-Depletion on Acute Lethal Infection of Mice with Trypanosome congolense (2008)
  • Author(s): Namangala B, Yokoyama N, Ikehara Y, Taguchi O, Tsujimura K, Sugimoto C, Inoue N.
  • Journal Title: J Vet Med Sci. 70(8) Pages: 751-759
• [Journal Article] Prevalence of Theileria equi and Babesia caballi infections in horses belonging to resource-poor farmers in the north-eastern Free State Province, South Africa (2008)
  • Author(s): Motloang MY, Thekisoe OM, Alhassan A, Bakheit M, Motheo MP, Masangane FE, Thibedi ML, Inoue N, Igarashi I, Sugimoto C, Mbati PA.
  • Journal Title: Onderstepoort J Vet Res. 75(2) Pages: 141-146
• [Journal Article] Identification and molecular characterization of a novel stage-specific surface protein of Trypanosoma congolense epimastigotes (2008)
  • Author(s): Sakurai T, Sugimoto C, Inoue N.
  • Journal Title: Mol Biochem Parasitol. 161 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Effect of CD4(+)CD25(+) T Cell-Depletion on Acute Lethal Infection of Mice with Trypanosoma congolense (2008)
  • Author(s): Namangala B, Yokoyama N, Ikehara Y, Taguchi O, Tsujimura K, Sugimoto C, Inoue N.
  • Journal Title: J Vet Med Sci. 70 Pages: 751-9
  • NAID: 110006878921
  • Peer Reviewed
• [Journal Article] Prevalence of Theileria equi and Babesia caballi infections in horses belonging to resource-poor farmers in the north-eastern Free State Province, South Africa (2008)
  • Author(s): Motloang MY, Thekisoe OM, Al hassan A, Bakheit M, Motheo M P, Masangane FE, Thibedi ML, Inoue N, Igarashi I, Sugimoto C, Mbati PA.
  • Journal Title: Onderstepoort J Vet Res. 75 Pages: 141-146
  • Peer Reviewed
• [Presentation] Surveillance of Human African Trypanosomiasis in Zambia and Characterization of Tyapanosoma brucei rhodesiense (2010)
  • Author(s): Sugimoto C.
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2010
  • Place of Presentation: Hanoi (Vietnam)
  • Year and Date: 2010-11-12
• [Presentation] Surveillance of Human African Trypanosomiasis in Zambia and Charact erization of Tyapanosoma brucei rhodesiense. (2010)
  • Author(s): Sugimoto, C.
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections 2010
  • Place of Presentation: Hanoi, Vietnam
  • Year and Date: 2010-11-12
• [Presentation] 次世代シークエンサーを活用したEhrlichia ruminantium弱毒ワクチン株の全ゲノム解析 (2010)
  • Author(s): 中尾亮
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-03-29
• [Presentation] 次世代シークエンサーを活用したEhrilichia ruminantium弱毒ワクチン株の全ゲノム解析 (2010)
  • Author(s): 中尾亮
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-03-29
• [Presentation] 次世代シークエンサーによるEhrlichia ruminantium弱毒ワクチン株の全長ゲノム解読と弱毒化機序の遺伝的背景の解析 (2010)
  • Author(s): 中尾亮
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 日本獣医生命科学大学(武蔵野市)
  • Year and Date: 2010-03-26
• [Presentation] Ehrlichia ruminantium多型解析のためのMulti-Locus Variable Number Tandem Repeat Analysis(MLVA)法の開発 (2010)
  • Author(s): 中尾亮
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学(帯広市)
• [Presentation] Whole-genome analysis of an attenuated Ehrlichia ruminantium vaccine strain using next-generation sequencer (2010)
  • Author(s): 中尾亮
  • Organizer: The 2nd International Young Researcher Seminar in Zoonosis Control 2010
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] Whole-genome analysis of an attenuated Ehrlichia ruminantium vaccine strain using next-generation sequencer (2010)
  • Author(s): 中尾亮
  • Organizer: XIIth International Congress of Parasitology (ICOPA)
  • Place of Presentation: Melbourne Convention & Exhibition Centre (Melbourne, Australia)
• [Presentation] 次世代シーケンサーによるEhrlichia ruminantium弱毒ワクチン株の全長ゲノム解読と弱毒化機序の遺伝的背景の解析 (2010)
  • Author(s): 中尾亮
  • Organizer: 第18回ダニと疾患のインターフェースに関するセミナー
  • Place of Presentation: トキ交流会館(新潟県佐渡市)
• [Presentation] Ehrlichia ruminantium多型解析のためのMulti-Locus Variable Number Tandem Repeat Analysis (MLUA)法の開発 (2010)
  • Author(s): 中尾亮
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学(帯広市)
• [Presentation] Ehrlichia ruminantium多型解析のためのMulti-Locus Variable Number Tandem Repeat Analysis (MLUA)法開発 (2010)
  • Author(s): 中尾亮
  • Organizer: 第56回日本寄生虫学会・日本衛生動物学会
  • Place of Presentation: 北海道大学(札幌市)
• [Presentation] LAMP法によるEhrlichia ruminantium迅速検出法の開発 (2009)
  • Author(s): 中尾亮
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: とりぎん文化会館(鳥取市)
  • Year and Date: 2009-09-26
• [Presentation] invited lecture "Development of loop-mediated isothermal amplification (LAMP) for diagnoses of trypanosomoses" (2009)
  • Author(s): Chihiro Sugimoto
  • Organizer: 45th Annual Meeting of Malaysian Society of Parasitology and Tropical Medicine
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Book] Theileria(Eds. V. Nene and C. Kole., Genome Mapping and Genomics in Animal-Associated Microbes.) (2009)
  • Author(s): Richard P. Bishop, David O. Odongo, David J. Mann, Terry W. Pearson, Chihiro Sugimoto, Lee R. Haines Elizabeth Glass, Kirsty Jensen, Ulrike Seitzer, Jabbar S. Ahmed, Simon P. Graham, Etienne P de Villiers
  • Publisher: Springer-Verlag
• [Book] Theileria. In : Genome Mapping and Genomics in Animal-Associated Microbes.(Eds. V. Nene and C. Kole) (2009)
  • Author(s): Richard P. Bishop, David O. Odongo, David J. Mann, Terry W. Pearson, Chihiro Sugimoto, Lee R. Haines, Elizabeth Glass, Kirsty Jensen, Ulrike Seitzer, Jabbar S. Ahmed, Simon P. Graham, Etienne P. de Villiers.
  • Total Pages: 41
  • Publisher: Springer-Verlag, Berlin Heidelberg, Germany
• [Remarks]
  • URL: http://www.hokudai.ac.jp/czc/collabed/
• [Remarks] ツェツェバエ完全長cDNAデータバンク公開
  • URL: http://fullmal.ims.u-tokyo.ac.jp/
• [Remarks] ツェツェバエ完全長c DNAデータベース
  • URL: http://fullmal.hgc.jp/index_arth.html
• [Remarks]
  • URL: http://fullmal.hgc.jp/index_arth.html