Molecular mechanism of activity-dependent gene expression and synapse formation

• Project/Area Number: 19300107
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: NOSE Akinao The University of Tokyo, 大学院・新領域創成科学研究科, 教授 (30260037)
• Project Period (FY): 2007 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: シナプス / ショウジョウバエ / 転写因子 / グルタミン酸受容体 / マイクロアレイ / 神経活動 / 遺伝子発現 / lola / 神経回路 / 運動神経 / 筋肉 / prospero

Research Abstract

マイクロアレイ技術を用い、ショウジョウバエの神経筋シナプスにおいて、シナプス形成の前後や有無に対応したシナプス後細胞(筋肉細胞)での遺伝子発現の変化を検出することにより、シナプス形成・成熟過程において誘導される遺伝子を系統的にスクリーニングし、さらに機能を調べた。その結果、神経支配により発現が抑制される遺伝子のひとつである、転写因子longitudinals lacking(lola)が、筋肉において、グルタミン酸受容体などの複数のシナプス機能分子の発現を転写レベルで制御することを見いだした。

Research Products

• [Journal Article] Drosophlla Teyrepresses transcription of a repulsive cue Toll and generates neuromuscular target specificity (2010)
  • Author(s): Inaki, M, Shinza-Kameda, M, Ismat, A., Frasch, M, Nose, A.
  • Journal Title: Development (inpress. (掲載確定))
• [Journal Article] Subunit-specific and homeostatic regulation of glutamate receptor localization by CaMKII in Drosophila neuromuscular junctions (2010)
  • Author(s): Morimoto T, Nobechi M, Komatsu A, Miyakawa H, Nose A.
  • Journal Title: Neuroscience 165 Pages: 1284-1292
• [Journal Article] Target recognition at the tips of postsynaptic filopodia : accumulation and function of Capricious (2009)
  • Author(s): Kohsaka, H, Nose, A.
  • Journal Title: Development 136 Pages: 1127-1135
• [Journal Article] Innervation and activity dependent dynamics of postsynaptic oxidative metabolism (2008)
  • Author(s): Kazama, H., Ichikawa, A, Kohsaka, H., Morimoto-Tanifuji, T., Nose, A.
  • Journal Title: Neuroscience 152 Pages: 40-49
• [Journal Article] Innervation and activity dependent dynamics of postsynaptic oxidative metabolism (2008)
  • Author(s): 風間北斗、市川彩子、高坂洋史、森本(谷藤)高子、能瀬聡直
  • Journal Title: Neuroscience 152 Pages: 40-49
  • Peer Reviewed
• [Journal Article] In vivo induction of postsynaptic molecular assembly by the cell adhesion molecule Fasciclin2 (2007)
  • Author(s): Kohsaka, H., Takasu, E., Nose, A.
  • Journal Title: J. Cell Biol 179 Pages: 1289-1300
• [Journal Article] Wnt4 is a local repulsive cue that determines synaptic target specificity (2007)
  • Author(s): Inaki, M., Yoshikawa, S., Thomas, J. B., Aburatani, H., Nose, A
  • Journal Title: Curr. Biol 17 Pages: 1574-1579
• [Journal Article] Synaptic components necessary for retrograde signaling triggered by calcium/calmodulin-dependent protein kinase II during synaptogenesis (2007)
  • Author(s): Kazama, H., Nose, A., Morimoto-Tanifuji, T.
  • Journal Title: Neuroscience 145 Pages: 1007-15
• [Journal Article] Wnt4 is a local repulsive cue that determines synaptic target specificity (2007)
  • Author(s): 稲木美紀子、Yoshikawa, S., Thomas, J. B., 油谷浩幸、能瀬聡直
  • Journal Title: Current Biology 17 Pages: 1574-1579
  • Peer Reviewed
• [Journal Article] In vivo induction of postsynaptic molecular assembly by the cell adhesion molecule Fasciclin2 (2007)
  • Author(s): 高坂洋史、高須悦子、能瀬聡直
  • Journal Title: Journal of Cell Biology 179 Pages: 1289-1300
  • Peer Reviewed
• [Presentation] Functional analysis of genes whose expression is regulated by nerve innervation during synaptogenesis (2009)
  • Author(s): Ai Fukui, Gaku Tonoe, Mikiko Inaki, Mizuho Homma, Takako Morimoto, Hiroyuki Aburatani, Akinao Nose
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Functional analysis of genes whose expression is regulated by nerve innervation during synaptogenesis (2009)
  • Author(s): Fukui, A., Tonoe, G., Inaki, M., Homma, M., Morimoto, T., Aburatani, H., Nose, A.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 古屋
• [Presentation] Single-cell analysis of innervation-dependent gene expression during synaptogenesis NeuroFly2008 (2008)
  • Author(s): Fukui, A., Tonoe, G., Inaki, M., Aburatani, H., Nose, A.
  • Organizer: 12th European Drosophila neurobiology conference
  • Place of Presentation: Wurzburg, Germany
• [Presentation] Single-cell analysis of innervation-dependent gene expression during synaptogenesis (2008)
  • Author(s): Fukui, A., Tonoe, G., Inaki, M., Aburatani, H., Nose, A.
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] Analysis of innervation-dependent geneexpression during synaptogenesis (2007)
  • Author(s): 福井愛, 稲木美紀子, 油谷浩幸, 能瀬聡亘
  • Organizer: 日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Analysis of innervation-dependent gene expression during synaptogenesis (2007)
  • Author(s): 福井愛, 稲木美紀子, 油谷浩幸, 能瀬聡直
  • Organizer: 日本神経科学大会
  • Place of Presentation: 横浜
• [Remarks]
  • URL: http://bio.phys.s.u-tokyo.ac.jp/