Neural circuit mechanisms of behavioral control through striatal projection pathways

• Project/Area Number: 19300109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Fukushima Medical University
• Principal Investigator: KOBAYASHI Kazuto Fukushima Medical University, 医学部, 教授 (90211903)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Kenta 福島県立医科大学, 医学部, 助教 (70315662) ; KAI Nobuyuki 福島県立医科大学, 医学部, 助教 (50301750) ; YASOSHIMA Yasunobu 福島県立医科大学, 医学部, 講師 (00273566)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2007: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 学習 / 線条体 / 投射ニューロン / ドーパミン伝達 / ドーパミン受容体 / 神経活動 / 遺伝子改変動物 / イムノトキシン細胞標的法 / 強化学習 / ドーパミンD1受容体 / 遺伝子改変マウス / 運動制御

Research Abstract

In the present study, we addressed the behavioral and physiological roles of these two pathways in the performance of conditional discrimination task. The analysis of the transgenic mice that deleted the striatonigral pathway indicated that the striatonigral pathway is mainly involved in the facilitation of speed of learned motor response but does not affect the accuracy of response selection. In addition, the analysis of the transgenic rats that lacked the striatopallidal pathway revealed that the striatopallidal pathway plays an important role in the accuracy of response selection but does not influence speed of motor response in conditional discrimination task.

Research Products

• [Journal Article] Molecular identity of periglomerular and short axon cells. (2010)
  • Author(s): Kiyokage E., Pan Y., Shao, Z., Kobayashi, K., Szabo, Y., Yanagawa, Y., Obata, K., Okano, H., Toida, K., Puche, A.C., Shipley. M.T.
  • Journal Title: J. Neurosci. 30(3) Pages: 1185-1196
  • Peer Reviewed
• [Journal Article] Synaptic input of ON-bipolar cells onto the dopaminergic neurons of the mouse retina. (2010)
  • Author(s): Contini, M., Lin, B., Kobayashi, K., Okano, H., Masland, R.H., Raviola, E.
  • Journal Title: J. Comp. Neurol. 518(11) Pages: 2035-2050
  • Peer Reviewed
• [Journal Article] 道具的学習と行動制御を研究するモデル実験動物 (2010)
  • Author(s): 小林和人, 深堀良二, 甲斐信行, 岡田佳奈, 小林とも子
  • Journal Title: 生体の科学 Vol.61,No.1 Pages: 47-52
• [Journal Article] Molecular identity of periglomerular and short axon cells (2010)
  • Author(s): Kiyokage E., et al.
  • Journal Title: J. Neurosci. 30 Pages: 1185-1196
  • Peer Reviewed
• [Journal Article] Synaptic input of ON-bipolar cells onto the dopaminergic neurons of the mouse retina (2010)
  • Author(s): Contini, M., et al.
  • Journal Title: J.Comp.Neurol. 519(in press)
  • Peer Reviewed
• [Journal Article] 道具的学習と行動制御を研究するモデル実験動物 (2010)
  • Author(s): 小林和人, 他
  • Journal Title: 生体の科学 61 Pages: 47-52
• [Journal Article] Interplay between cytosolic dopamine, calcium, and alpha-synuclein causes selective death of substantia nigra neurons. (2009)
  • Author(s): Mosharov EV, Larsen KE, Kanter E, Phillips KA, Wilson K, Schmitz Y, Krantz DE, Kobayashi K, Edwards RH, Sulzer D.
  • Journal Title: Neuron 62(2) Pages: 218-229
  • Peer Reviewed
• [Journal Article] Unexpected expression of a-and b-globin in mesencephalic dopaminergic neurons and glial cells. (2009)
  • Author(s): Biagioli, M., Pinto, M., Cesselli, D., Zaninello, M., Lazarevic, D., Simone, R., Vlachouli, C., Plessy, C., Bertin, N., Beltrami, A., Kobayashi, K., Gallo, V., Ferrer, I., Santoro, C., Rivella, S., Beltrami, C.A., Carninci, P., Raviola, E., Gustincich, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA 106(36) Pages: 15446-15451
  • Peer Reviewed
• [Journal Article] Ephrin-A5 regulates the formation of the ascending midbrain dopaminergic pathways. (2009)
  • Author(s): Cooper, M.A., Kobayashi, K., Zhou, R.
  • Journal Title: Dev. Neurobiol. 69(1) Pages: 36-46
  • Peer Reviewed
• [Journal Article] Identification of an activator protein-1-like Sequence as the glucocorticoid response element in the rat tyrosine hydroxylase gene. (2009)
  • Author(s): Sheela Rani, C.S., Elango, N., Wang, S.-S., Kobayashi, K., Strong, R.
  • Journal Title: Mol. Pharmacol. 75(3) Pages: 589-598
  • Peer Reviewed
• [Journal Article] イムノトキシン細胞標的法を用いた直接路・間接路の機能解析 (2009)
  • Author(s): 深堀良二, 小林和人
  • Journal Title: Brain and Nerve Vol.61,No.4 Pages: 412-418
• [Journal Article] Interplay between cytosolic dopamine, calcium, and alpha-synuclein causes selective death of substantia nigra neurons (2009)
  • Author(s): Mosharov, E.V., et al.
  • Journal Title: Neuron 62 Pages: 218-229
  • Peer Reviewed
• [Journal Article] Unexpected expression of α- and β-globin in mesencephalic dopaminergic neurons and glial cells (2009)
  • Author(s): Biagioli, M., et al.
  • Journal Title: Proc.Natl.Acad. Sci.USA 106 Pages: 15446-15451
  • Peer Reviewed
• [Journal Article] イムノトキシン細胞標的法を用いた直接路・間接路の機能解析 (2009)
  • Author(s): 深堀良二、小林和人
  • Journal Title: Brain and Nerve 61 Pages: 412-418
• [Journal Article] Identification of an activator protein-1-like Sequence as the glucocorticoid response element in the rat tyrosine hydroxylase gene. (2009)
  • Author(s): Sheela Rani, C. S., et al.
  • Journal Title: Mol. Pharmacol. 75 Pages: 589-598
  • Peer Reviewed
• [Journal Article] Selective loss of nigral dopamine neurons induced by overexpression of truncated human a-synuclein in mice. (2008)
  • Author(s): Wakamatsu, M., Ishii, A., Iwata, S., Sakagami, J., Ukai, Y., Ono, M., Kanbe, D., Muramatsu, S.I., Kobayashi, K., Iwatsubo, T., Yoshimoto, M.
  • Journal Title: Neurobiol. Aging 29(4) Pages: 574-585
  • Peer Reviewed
• [Journal Article] Transient silencing of synaptic transmitter release from specific neuronal types by recombinant tetanus toxin light chain fused to antibody variable region. (2008)
  • Author(s): Kobayashi, T., Kai, N., Kobayashi, K., Fujiwara, T., Akagawa, I., Onda, M., Kobayashi, K.
  • Journal Title: J. Neurosci. Methods 175(1) Pages: 125-132
  • Peer Reviewed
• [Journal Article] イムノトキシン細胞標的法 (2008)
  • Author(s): 小林和人, 深堀良二
  • Journal Title: 実験医学(増刊号) Vol.26,No.12 Pages: 195-202
• [Journal Article] ドーパミン伝達による行動発現システムの制御 (2008)
  • Author(s): 甲斐信行, 小林和人
  • Journal Title: 蛋白質核酸酵素(増刊号:神経の分化、回路形成、機能発現) Vol.53,No.4 Pages: 565-572
• [Journal Article] Selective loss of nigral dopamine neurons induced by overexpression of truncated human α-synuclein in mice (2008)
  • Author(s): Wakamatsu, M., et al.
  • Journal Title: Neurobiol. Aging 29 Pages: 574-585
  • Peer Reviewed
• [Journal Article] Transient silencing of synaptic transmitter release from specific neuronal types by recombinant tetanus toxin light chain fused to antibody variable region (2008)
  • Author(s): Kobayashi, T., et al.
  • Journal Title: J. Neurosci. Methods 175 Pages: 125-132
  • Peer Reviewed
• [Journal Article] Ephrin-A5 regulates the formation of the ascending midbrain dopaminergic pathways (2008)
  • Author(s): Cooper, M. A., et al.
  • Journal Title: Dev. Neurobiol. 69 Pages: 36-46
  • Peer Reviewed
• [Journal Article] イムノトキシン細胞標的法 (2008)
  • Author(s): 小林和人, 深堀良二
  • Journal Title: 実験医学 26 Pages: 195-202
• [Journal Article] 基底核と記憶 (2008)
  • Author(s): 甲斐信行, 小林和人
  • Journal Title: 分子精神医学 8 Pages: 10-15
• [Journal Article] ドーパミン伝達による行動発現システムの制御 (2008)
  • Author(s): 甲斐信行、小林和人
  • Journal Title: 蛋白質核酸酵素 53 Pages: 565-572
• [Journal Article] Controlled cell targeting system to study the brain neural circuitry. (2007)
  • Author(s): Kobayashi, K.
  • Journal Title: Neurosci. Res 58(2) Pages: 118-123
  • NAID: 10024414871
  • Peer Reviewed
• [Journal Article] Compartment-specific modulation of GABAergic synaptic transmission by m-opioid receptor in the mouse striatum with green fluorescent protein-expressing dopamine islands. (2007)
  • Author(s): Miura, M., Saino-Saito, S., Masuda, M., Kobayashi, K., Aosaki, T.
  • Journal Title: J. Neurosci. 27(36) Pages: 9721-9728
  • Peer Reviewed
• [Journal Article] Efficient gene transfer via retrograde transport in rodent and primate brains using a human immunodeficiency virus type 1-based vector pseudotyped with rabies virus glycoprotein. (2007)
  • Author(s): Kato, S., Inoue, K., Kobayashi, K., Yasoshima, Y., Miyachi, S., Inoue, S., Hanawa, H., Shimada, T., Takada, M., Kobayashi, K.
  • Journal Title: Human Gene Ther. 18(11) Pages: 1141-1152
  • Peer Reviewed
• [Journal Article] Controlled cell targeting system to study the brain neural circuitry (2007)
  • Author(s): Kobayashi, K.
  • Journal Title: Neurosci. Res. 58 Pages: 118-123
  • NAID: 10024414871
  • Peer Reviewed
• [Journal Article] Efficient gene transfer via retrograde transport in rodent and primate brains using a human immunodeficiency virus type 1-based vector pseudotyped with rabies virus glycoprotein (2007)
  • Author(s): Kato, S, et. al.
  • Journal Title: Human Gene Ther. 18 Pages: 1141-1152
  • Peer Reviewed
• [Journal Article] Compartment-specific modulation of GABAergic synaptic transmission by μ-opioid receptor in the mouse striatum with green fluorescent protein-expressing dopamine islands (2007)
  • Author(s): Miura, M., et. al.
  • Journal Title: J. Neurosci. 27 Pages: 9721-9728
  • Peer Reviewed
• [Journal Article] Visceral afferents directly activate catecholamine neurons in the solitary tract nucleus (2007)
  • Author(s): Appleyard, S.M., et. al.
  • Journal Title: J. Neurosci. 27 Pages: 13292-13302
  • Peer Reviewed
• [Journal Article] ニューロンネットワーク解析の新技術-組換え体イムノトキシンを利用した細胞標的法 (2007)
  • Author(s): 小林和人
  • Journal Title: 化学と生物 45 Pages: 16-20
• [Presentation] Generating animal models for the central nervous system diseases by immunotoxin cell targeting (2010)
  • Author(s): Kobayashi, K., Okada, K.
  • Organizer: 2^<nd> International Congress of Antibodies
  • Place of Presentation: Beijing
  • Year and Date: 2010-03-26
• [Presentation] Generating animal models for the central nervous system diseases by immunotoxin cell targeting. (2010)
  • Author(s): Kobayashi K, Okada K.
  • Organizer: 2nd International Congress of Antibodies.
  • Place of Presentation: Beijing
• [Presentation] Implication of hippocampal dopamine in the conditioning of the rewarding effect of cocaine (2009)
  • Author(s): Tanaka, T, et al.
  • Organizer: 39^<th> Annual Meeting of Society for Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-18
• [Presentation] 線条体におけるドーパミンD2受容体発現ニューロンの運動機能 (2009)
  • Author(s): 佐野裕美, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] 強化学習における線条体-黒質神経路の行動学的役割 (2009)
  • Author(s): 深堀良二, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] コカイン誘発報酬効果に対する海馬領域におけるドパミンの役割 (2009)
  • Author(s): 田中智子, 他
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] Roles of the striatonigral neural pathway in basal ganglia circuitry in two-choice reaction time task (2009)
  • Author(s): Fukabori, R., et al
  • Organizer: 4th Symposium for Molecular and Cellular Cognition Society-Asia
  • Place of Presentation: Yokohama
  • Year and Date: 2009-09-15
• [Presentation] Neural circuit mechanisms underlying dopamine-dependent behaviors. Multidisciplinary approaches to basal ganglia functions (2009)
  • Author(s): Kobayashi, K.
  • Organizer: XXXVI International Congress of Physiological Sciences (IUPS)
  • Place of Presentation: Kyoto
  • Year and Date: 2009-07-29
• [Presentation] Roles of the striatonigral neural pathway in basal ganglia circuitry in two-choice reaction time task. (2009)
  • Author(s): Fukabori R, Kai N, Kobayashi K, Yasoshima Y, Tsutsui Y, Kobayashi K.
  • Organizer: 4th symposium for Molecular and Cellular Cognition Society-Asia
  • Place of Presentation: Yokohama
• [Presentation] Implication of hippocampal dopamine in the conditioning of the rewarding effect of cocaine. (2009)
  • Author(s): Tanaka T, Kai N, Kobayashi K, Hironaka N.
  • Organizer: 39th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Chicago.
• [Presentation] Behavioral roles of the striatonigral neural pathway in reinforcement learning (2009)
  • Author(s): Fukabori R, Kai N, Kobayashi K, Yasoshima Y, Tsutsui Y, Kobayashi K.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Motor functions of dopamine D2 receptorexpressing neurons in the striatum. (2009)
  • Author(s): Sano H, Chiken S, Kobayashi K, Nambu A.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Neural circuit mechanisms underlying dopamine-dependent behaviors. Multidisciplinary approaches to basal ganglia functions. (2009)
  • Author(s): Kobayashi K.
  • Organizer: XXXVI International Congress of Physiological Sciences (IUPS)
  • Place of Presentation: Kyoto
• [Presentation] Behavioral and physiological analysis of the striatopallidal neural pathway by conditional transmission silencing. (2008)
  • Author(s): Kobayashi K, Kobayashi T, Kai N, Kobayashi K, Fujiwara T, Akagawa K, Onda M, Pastan I.
  • Organizer: 38th Annual Meeting of Society for Neuroscience
  • Place of Presentation: Washington DC
• [Presentation] Behavioral and physiological analysis of the striatopallidal neural path way by conditional transmission silencing (2008)
  • Author(s): Kobayashi, K., et al.
  • Organizer: 38^<th> Annual Meeting of Society for Neuro'science
  • Place of Presentation: Washington DC
• [Presentation] Behavioral and physiological roles of the striatonigral neural pathway in motor control (2008)
  • Author(s): Fukabori, R., et al.
  • Organizer: 第31回日本神経科学学会
  • Place of Presentation: 東京
• [Presentation] Essential role of the striatonigral neural pathway in motor behavior revealed by conditional ablation of dopamine D1 receptor-containing neurons in the dorsal striatum (2007)
  • Author(s): Fukabori, R., et. al.
  • Organizer: 2nd Molecular and Cellular Cognition Society-Asia
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2007-09-08
• [Presentation] Conditional ablation of striatal neuronal types containing dopamine D_1 receptors by using immunotoxin cell targeting (2007)
  • Author(s): Kobayashi, K., et. al.
  • Organizer: Dopamine 50 Years
  • Place of Presentation: Gotenborg, Sweden
  • Year and Date: 2007-06-01
• [Presentation] Conditional ablation of striatal neuronal types containing dopamine D1 receptors by using immunotoxin cell targeting. (2007)
  • Author(s): Kobayashi K, Kai N, Yasoshima Y, Fukabori R
  • Organizer: Dopamine 50 Years
  • Place of Presentation: Gotenborg