Analysis of the locomotor circuit formation in zebrafish spinal cord

• Project/Area Number: 19300118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institutes of Natural Sciences Okazaki Research Facilities
• Principal Investigator: HIGASHIJIMA Shin-ichi National Institutes of Natural Sciences Okazaki Research Facilities, 岡崎統合バイオサイエンスセンター, 准教授 (80270479)
• Project Period (FY): 2007 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2008: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2007: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: ゼブラフィッシュ / 神経分化 / 神経回路 / トランスジェニック / 脊髄 / 非対称分裂

Research Abstract

The p2 progenitor domain in the ventral spinal cord gives rise to two interneuron subtypes, V2a and V2b. The mechanism as to how V2a and V2b neurons arise from a single progenitor domain was unknown. We showed in this study that p2 progenitors divide once to produce V2a/V2b neuron pairs, indicating that V2a and V2b neurons are generated by the asymmetric division of pair-producing progenitor cells. We also showed that Delta-Notch interactions between sister cells play a critical role in the final outcome of these asymmetric divisions. This mechanism for determining cell fate is similar to asymmetric divisions that occur during Drosophila neurogenesis, where ganglion mother cells divide once to produce distinct neurons. However, unlike in Drosophila, the divisional axes of p2 progenitors in zebrafish were not fixed. We report that the terminal division of pair-producing progenitor cells in vertebrate neurogenesis can reproducibly produce two distinct neurons through a mechanism that may not depend on the orientation of the division axis.

Research Products

• [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum. (2010)
  • Author(s): Kani, S., Bae, Y-K, Shimizu T, Tanabe, K., Satou, C., Parsons, M., Scott, E., Baier, H., Higashijima, S., Hibi, M.
  • Journal Title: Developmental Biology (in press)
  • Peer Reviewed
• [Journal Article] Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish. (2010)
  • Author(s): Wada, H., Ghysen, A., Satou, C., Higashijima, S, Kawakami, K., Hamaguchi, S., Sakaizumi, M.
  • Journal Title: Developmental Biology 340 Pages: 583-594
  • Peer Reviewed
• [Journal Article] Illuminating Cell-Cycle Progression in the Developing Zebrafish Embryo. (2009)
  • Author(s): Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S., Miyawaki, A.
  • Journal Title: Proc. Natl. Acad. Sci. (USA) 106 Pages: 20812-20817
  • Peer Reviewed
• [Journal Article] Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish. (2009)
  • Author(s): Satou, C., Kimura, Y., Kohashi, T., Horikawa, K., Takeda, H., Oda, Y., Higashijima, S.
  • Journal Title: J. Neuroscience 29 Pages: 6780-6793
  • Peer Reviewed
• [Journal Article] Anatomy of zebrafish cerebellum and screen for mutations affecting its development. (2009)
  • Author(s): Bae, Y., Kani, S., Shimizu, T., Tanabe, K., Nojima, H., Kimura, Y., Higashijima, S., Hibi, M.
  • Journal Title: Developmental Biology 300 Pages: 406-426
  • Peer Reviewed
• [Journal Article] From the Olfactory Bulb to Higher Brain Centers: Genetic Visualization of Secondary Olfactory Pathways in Zebrafish. (2009)
  • Author(s): Miyasaka, N., Morimoto, K., Tsubokawa, T., Higashijima, S., Okamoto, H., Yoshihara, Y.
  • Journal Title: J. Neuroscience 29 Pages: 4756-4767
  • Peer Reviewed
• [Journal Article] Vsx2 in the zebrafish retina: restricted lineages through depression. (2009)
  • Author(s): Vitorino, M., Jusf, P.R., Maurus, D., Kimura, Y., Higashijima, S., Harris, W.A.
  • Journal Title: Neural Development 4 Pages: 14-14
  • Peer Reviewed
• [Journal Article] Functional role of a specialized class of spinal commissural inhibitory neurons during fast escapes in zebrafish (2009)
  • Author(s): Satou, C.
  • Journal Title: Journal of Neuroscience 29 Pages: 6780-6793
  • Peer Reviewed
• [Journal Article] From the Olfactory Bulb to Higher Brain Centers : Genetic Visualization of Secondary Olfactory Pathways in Zebrafish (2009)
  • Author(s): Miyasaka, N.
  • Journal Title: Journal of Neuroscience 29 Pages: 4756-4767
  • Peer Reviewed
• [Journal Article] Illuminating Cell-Cycle Progression in the Developing Zebrafish Embryo (2009)
  • Author(s): Sugiyama, M.
  • Journal Title: Proc.Natl.Acad.Sci.(USA) 106 Pages: 20812-20817
  • Peer Reviewed
• [Journal Article] Mutation in the abdb gene causes abnormal iron and fatty acid metabolism in developing medaka fish. (2008)
  • Author(s): Miyake, A., Higashijima, S., Kobayashi, D., Narita, T, Jindo, T., Setiamarga, D.H.E., Ohisa, S., Orihara, N, Hibiya, K., Konnno, S., Sakaguchi, S., Hoie, K., Imai, Y., Naruse, K., Kudo, A., Takeda, H.
  • Journal Title: Development, Growth & Differentiation 50 Pages: 703-716
  • Peer Reviewed
• [Journal Article] V2a and V2b neurons are generated by the final divisions of pair-producing progenitors in the zebrafish spinal cord. (2008)
  • Author(s): Kimura, Y., Satou, C., Higashijima, S.
  • Journal Title: Development 135 Pages: 3001-3005
  • Peer Reviewed
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs. (2008)
  • Author(s): Hossain, Md. I., Iwasaki, H., Okouchi, Y., Chahine, M., Higashijima, S., Nagayama, K., Okamura, Y.
  • Journal Title: J. Biol. Chem 283 Pages: 18248-59
  • Peer Reviewed
• [Journal Article] V2a and V2b neurons are generated by the final divisions of pair-producing progenitors in the zebrafish spinal cord (2008)
  • Author(s): Kimura, Y.
  • Journal Title: Development 135 Pages: 3001-3005
  • Peer Reviewed
• [Journal Article] Enzyme domain affects the movement of the voltage sensor in ascidian and zebrafish VSPs (2008)
  • Author(s): Hossain, Md. I.
  • Journal Title: J. Biol. Chem. 283 Pages: 18248-18259
  • Peer Reviewed
• [Journal Article] Mutation in the abdb gene causes abnormal iron and fatty acid metabolism in developing medaka fish (2008)
  • Author(s): Miyake, A.
  • Journal Title: Development, Growth & Differentiation 50 Pages: 703-716
  • Peer Reviewed
• [Journal Article] A topographic map of recruitment in spinal cord. (2007)
  • Author(s): McLean, D.L., Fan, J., Higashijima, S., Hale, M.E., Fetcho, J.R.
  • Journal Title: Nature 446 Pages: 71-5
  • Peer Reviewed
• [Journal Article] A topographic map of recruitment in spinal cord (2007)
  • Author(s): McLean, D.L., Fan, J., Higashijima, S., Hale, M.E. & Fetcho, J.R.
  • Journal Title: Nature 446 Pages: 71-5
  • Peer Reviewed
• [Journal Article] Zebrafish and motor control over the last decade (2007)
  • Author(s): Fetcho, J.R., Higashijima, S., & McLean.D.L.
  • Journal Title: Brain Research Reviews 57 Pages: 86-93
• [Journal Article] in vivoイメージングのためのプローブ〜ゼブラフィッシュの蛍光イメージング (2007)
  • Author(s): 木村 有希子、佐藤 千恵、東島 眞一
  • Journal Title: 実験医学増刊号蛍光・発光試薬の選び方と使い方 Pages: 125-130
• [Presentation] ゼブラフィッシュを用いたin vivoイメージング (2009)
  • Author(s): 東島眞一
  • Organizer: 第32回日本分子生物学会年会ワークショップ
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Development and function of spinal locomotor circuits in zebrafish (2009)
  • Author(s): S. Higashijima
  • Organizer: 3rd Viktor Hamburger Symposium
  • Place of Presentation: Chicago, USA
  • Year and Date: 2009-10-16
• [Presentation] Development and function of spinal locomotor circuits in zebrafish (2009)
  • Author(s): S Higashijima, C Satou, Y Kimura
  • Organizer: The 32th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2009-09-16
• [Presentation] Development and function of spinal locomotor circuits in zebrafish (2009)
  • Author(s): S Higashijima, Y Kimura, C Satou
  • Organizer: IUPS2009
  • Place of Presentation: Kyoto Japan
  • Year and Date: 2009-07-31
• [Presentation] V2a and V2b neurons are generated by the final divisions of pair-producing progenitors. (2008)
  • Author(s): Kimura Y, Satou C, Higashijima S
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-18
• [Presentation] Analysis of spinal V0 neurons with transgenic fish. (2008)
  • Author(s): Satou C, Kimura Y, Higashijima S
  • Organizer: Neuroscience 2008
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2008-11-17
• [Presentation] ゼブラフィッシュのロコモーションの成魚に関わる脊髄神経回路の分子的基盤 (2008)
  • Author(s): 東島眞一, 木村有希子, 佐藤千恵
  • Organizer: 第80回日本遺伝学会ワークショップ
  • Place of Presentation: 名古屋
  • Year and Date: 2008-09-04
• [Presentation] Properties and functions of spinal V0 neurons in zebrafish. (2008)
  • Author(s): Satou C, Kimura Y, Higashijima S
  • Organizer: Neuro2008
  • Place of Presentation: Tokyo
  • Year and Date: 2008-07-09
• [Presentation] Development and function of spinal locomotor circuits in zebrafish (2007)
  • Author(s): 東島眞一, 木村有希子, 佐藤千恵
  • Organizer: 第30回日本神経科学大会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Presentation] Development and function of spinal locomotor circuits in zebrafish (2007)
  • Author(s): Higashijima, S., Kimura, Y.& Satou, C.
  • Organizer: 第30回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2007-09-11
• [Book] 細胞工学 (2009)
  • Author(s): 東島眞一
  • Total Pages: 107
  • Publisher: 秀潤社
• [Book] Development, Growth & Differentiation(Transgenic zebrafish expressing fluorescent proteins in central nervous system neurons.) (2008)
  • Author(s): Higashijima, S.
• [Book] Brain Research Reviews(Zebrafish and motor control over the last decade.) (2008)
  • Author(s): Fetcho, J. R., Higashijima, S., McLean, D. L.
• [Book] 実験医学増刊号 蛍光・発光試薬の選び方と使い方(in vivoイメージングのためのプローブ～ゼブラフィッシュの蛍光イメージング) (2007)
  • Author(s): 木村有希子、佐藤千恵、東島眞一
  • Publisher: 羊土社