| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2007: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Research Abstract |
The ultimate aim of the present study is to clarify how glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) in the brain respond to hormonal milieu fluctuated during stress response and circadian rhythm by directly visualizing these receptors as a whole brain level. As a preliminary study, we analyzed dynamics of GR using primary cultured neurons obtained from GFP-GR knock-in mice, and furthermore, we observed a fixed brain from GFP-GR knock-in mouse as a whole brain level using a two-photon laser microscope. We succeeded a direct visualization of GFP-GR at the depth of layer II of cerebral cortex of GFP-GR knock-in mouse.
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