Real-time imaging of stress-related receptor dynamics in the brain
Project/Area Number |
19300120
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Nerve anatomy/Neuropathology
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
NISHI Mayumi Kyoto Prefectural University of Medicine, 医学部, 教授 (40295639)
|
Co-Investigator(Kenkyū-buntansha) |
金城 政孝 北海道大学, 大学院・先端生命科学研究院, 教授 (70177971)
藤川 和世 京都府立医科大学, 医学研究科, プロジェクト研究員 (40433247)
|
Project Period (FY) |
2007 – 2009
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Project Status |
Completed (Fiscal Year 2009)
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Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2007: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
Keywords | GFP / GR / MR / ノックインマウス / in vivoイメージング / 海馬 / ストレス / 核輸送 / in vivo imaging / グルココルチコイド受容体 / ミネラルコルチコイド受容体 / ストレス応答 / 日内変動 |
Research Abstract |
The ultimate aim of the present study is to clarify how glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) in the brain respond to hormonal milieu fluctuated during stress response and circadian rhythm by directly visualizing these receptors as a whole brain level. As a preliminary study, we analyzed dynamics of GR using primary cultured neurons obtained from GFP-GR knock-in mice, and furthermore, we observed a fixed brain from GFP-GR knock-in mouse as a whole brain level using a two-photon laser microscope. We succeeded a direct visualization of GFP-GR at the depth of layer II of cerebral cortex of GFP-GR knock-in mouse.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] The A-and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription.2008
Author(s)
Shimi T, Pfleghaar K, Kojima S, Pack CG, Solovei I, Goldman AE, Adam SA, Shumaker DK, Kinjo M, Cramer T, Goldman RD
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Journal Title
Genes Dev. 22
Pages: 3409-21
Related Report
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[Journal Article] Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking.2008
Author(s)
Noda Y, Horikawa S, Kanda E, Yamashita M, Meng H, Eto K, Li Y, Kuwahara M, Hirai K, Pack C, Kinjo M, Okabe S, Sasaki S
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Journal Title
J Cell Biol. 182
Pages: 587-601
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[Journal Article] The novel cargo Alcadein induces vesicle association of kinesin-1 motor components and activates axonal transport.2007
Author(s)
Araki Y, Kawano T, Taru H, Saito Y, Wada S, Miyamoto K, Kobayashi H, Ishikawa HO, Ohsugi Y, Yamamoto T, Matsuno K, Kinjo M, Suzuki T
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Journal Title
EMBO J. 26
Pages: 1475-86
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[Journal Article] Direct visualization of glucocorticoid receptor positive cells in the hippocampal regions using green fluorescent protein transgenic mice2007
Author(s)
Nishi, M., Usuku, T., Itose, M., Fujikawa, K., Hosokawa, K., Matsuda, K-I., & Kawata, M
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Journal Title
Neuroscience 146
Pages: 1555-1560
Related Report
Peer Reviewed
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[Journal Article] Expression of GPR 30, a G-protein coupled membrane estrogen receptor in oxytocin neurons of the rat paraventricular and supraoptic nuclei.2007
Author(s)
Sakamoto, H., Matsuda, K., Hosokawa, K., Nishi, M., Morris, J., & Kawata, M
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Journal Title
Endocrinology 148
Pages: 5842-5850
Related Report
Peer Reviewed
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