The differentiation and function of molecular-layer interneurons in the cerebellum

• Project/Area Number: 19300127
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAKANO Kohichi Tokyo Medical and Dental University, 大学院・疾患生命科学研究部, 教授 (80171750)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2007: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 小脳 / 抑制性神経細胞 / ノッチ / 遺伝子欠損マウス / 籠細胞 / 星状細胞 / 抑制性ニューロン / 発達 / グリア細胞

Research Abstract

小脳は運動学習の中枢であり、その機能的役割は詳細に解析されてきたが、小脳形成過程の分子機序は不明な点が多い。我々は、細胞特異的遺伝子改変マウスを用い、小脳抑制性介在神経細胞の神経前駆細胞において、RBP-Jシグナルは神経前駆細胞から神経細胞への分化を促進する役割をもつことを明らかにした。さらに、小脳抑制性介在神経細胞を同定できるマーカーの組み合わせを明らかにした。

Research Products

• [Journal Article] Development of the mouse amygdala as revealed by enhanced green fluorescent protein gene transfer by means of in utero electroporation (2009)
  • Author(s): Soma, M., Aizawa, H., Ito, Y., Maekawa,M., Osumi, N., Nakahira, E., Okamoto,H., Tanaka, K., Yuasa, S.
  • Journal Title: J Comp Neurol 513 Pages: 113-128
  • Peer Reviewed
• [Journal Article] Development of the mouse amygdala as revealed by Enhanced green fluorescent protein gene transfer by means of in utero electroporation. (2009)
  • Author(s): Soma, M.
  • Journal Title: J Comp Neurol 513 Pages: 113-128
  • Peer Reviewed
• [Journal Article] Loss of Glial Glutamate and Aspartate Transporter(Excitatory Amino Acid Transporter 1)Causes Locomotor Hyperactivity and Exaggerated Responses to Psychotomimetics : Rescus by Haloperidol and Metabotropic Glutamate 2/3 Agonist (2008)
  • Author(s): Karlsson, RM., Tanaka, K., Heilig, M., Holmes, A.
  • Journal Title: Biol Psychiatry 64 Pages: 810-814
  • Peer Reviewed
• [Journal Article] Glutamate transporters regulate lesion-induced plasticity in the developing somatosensory cortex (2008)
  • Author(s): Takasaki, C., Okada, R., Mitani, A., Fukaya, M., Yamasaki, M., Fujihara, Y., Shirakawa, T., Tanaka, K., Watanabe, M.
  • Journal Title: J Neurosci 28 Pages: 4995-5006
  • Peer Reviewed
• [Journal Article] グルタミン酸トランスポーターの中枢神経系における役割 (2008)
  • Author(s): 相田知海、田中光一
  • Journal Title: Clinical Neuroscience 26 Pages: 1072-1076
• [Journal Article] グルタミン酸トランスポーターと精神神経疾患 (2008)
  • Author(s): 相田知海、田中光一
  • Journal Title: 遺伝子医学MOOK 12 Pages: 212-217
  • Peer Reviewed
• [Journal Article] アストロサイトにおけるグルタミン酸トランスポーターの機能 (2008)
  • Author(s): 田中光一
  • Journal Title: Brain and Nerve 59 Pages: 677-688
• [Journal Article] 神経幹細胞の分化過程のメカニズム (2008)
  • Author(s): 田中光一
  • Journal Title: 分子精神医学 7 Pages: 86-91
• [Journal Article] Behavioral characterization of GLT1(+/-) mice as a model of mild glutamatergic hyperfunction. (2008)
  • Author(s): Kiryk, A.
  • Journal Title: Neurotox Res 13 Pages: 19-30
  • Peer Reviewed
• [Journal Article] Glutamate transporters regulate lesion-induced Plasticity in the developing somatosensory cortex. (2008)
  • Author(s): Takasaki C.
  • Journal Title: J Neurosci 28 Pages: 4955-5006
  • Peer Reviewed
• [Journal Article] A loss of glial glutamate and aspartate transporter (Excitatory amino acid transporter 1) causes locomotor hyperactivity and exaggerated responses to psychomimetics : Rescue by haloperidol and metabotropic glutamate 2/3 agonist. (2008)
  • Author(s): Karlsson RM.
  • Journal Title: Biol Psychiatry 64 Pages: 810-814
  • Peer Reviewed
• [Journal Article] characterization of GLT1(+/-)mice as a model of mild glutamatergic hyperfunction (2007)
  • Author(s): Kiryk, A., Aida, T., Tanaka, K., Banerjee, P., Wilczynski, G.M., Meyza,K., Knapska, E., Filipkowaski, R.K., Kaczmarek, L., Danysz, W. Behavioral
  • Journal Title: Neurotox Res 13 Pages: 19-30
  • Peer Reviewed
• [Journal Article] activation of metabotropic glutamate receptors in cerebellar Purkinje cells in glutamate transporter EAAT4-deficient mice (2007)
  • Author(s): Nikkuni, O., Takayasu, Y., Iino, M., Tanaka, K., Ozawa, S. Facilitated
  • Journal Title: Neurosci Res 59 Pages: 296-303
  • Peer Reviewed
• [Journal Article] The monolayer formation of Bergmann glial cells is regulated by Notch/RBP-J signaling (2007)
  • Author(s): Komine, O., Nagaoka, M., Watase, K., Gutmann, D.H., Tanigaki, K., Honjo, T., Radtke, F., Saito, C., Chiba, S., Tanaka, K.
  • Journal Title: Dev Biol 311 Pages: 238-250
  • Peer Reviewed
• [Journal Article] Proteomics in retina of Excitory Acid Carrier 1(EAAC1)knockout mice (2007)
  • Author(s): Tanimoto, S., Suzuki, M., Ue, T., Okumichi, H., Aoyama, H., Souchelnytskyi, S., Tanaka, K., Agarwal, N., Mishima, H.K., Kanamoto, T.
  • Journal Title: Proteome Science 5 Pages: 13-13
  • Peer Reviewed
• [Journal Article] Activation of cholecystokinin neurons in the dorsal pallium of the telencephalon is indispensable for the acquisition of chick imprinting behavior (2007)
  • Author(s): Maekawa, F., Nakamori, T., Uchimura,M., Fujiwara, K., Yada, T., Tsukahara,S., Kanamatsu, T., Tanaka, K., Ohki-Hamazaki, H
  • Journal Title: J Neurochem 102 Pages: 645-1657
  • Peer Reviewed
• [Journal Article] The potential role of glutamate transporters in the pathogenesis of normal tension glaucoma (2007)
  • Author(s): Harada, T., Harada, C., Nakamura, K.,Qua, H-M.A., Okumura, A., Namekata, K., Saeki, T., Aihara, M., Yoshida, H., Mitani, A., Tanaka, K
  • Journal Title: J Clin Invest 117 Pages: 1763-1770
  • Peer Reviewed
• [Journal Article] Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses (2007)
  • Author(s): Takatsuru, Y., Iino, M., Tanaka, K., Ozawa, S
  • Journal Title: Neurosci Lett 420 Pages: 85-89
  • Peer Reviewed
• [Journal Article] Contribution of glutamate transporter GLT-1 to removal of synaptically released glutamate at climbing fiber-Purkinje cell synapses. (2007)
  • Author(s): Takatsuru, Y.
  • Journal Title: Neurosci Lett 420 Pages: 85-89
  • Peer Reviewed
• [Journal Article] Facilitated activation of metabotropic glutamate receptors in cerebellar Purkinje cells in glutamate transporter EAAT4-deficient mice. (2007)
  • Author(s): Nikkuni, O.
  • Journal Title: Neurosci Res 59 Pages: 296-303
  • Peer Reviewed
• [Journal Article] The potential role of glutamate transporters in the pathogenesis of normal tension glaucoma. (2007)
  • Author(s): Harada, T.
  • Journal Title: J Clin Invest 117 Pages: 1763-1770
  • Peer Reviewed
• [Journal Article] The monolayer formation of Bergmann glial cells is regulated by Notch/RBP-J signaling. (2007)
  • Author(s): Komine, O.
  • Journal Title: Dev Biol 311 Pages: 238-250
  • Peer Reviewed
• [Journal Article] Proteomics in retina of Excitory Acid Carrier 1 (EAAC1) knockout mice. (2007)
  • Author(s): Tanimoto, S.
  • Journal Title: Proteome Science 5 Pages: 13-18
  • Peer Reviewed
• [Journal Article] Activation of cholecystokinin neurons in the dorsal pallium of the telencephalon is indispensable for the acquisition of chick imprinting behavior. (2007)
  • Author(s): Maekawa, F.
  • Journal Title: J Neurochem 102 Pages: 1645-1657
  • Peer Reviewed
• [Journal Article] Assessment of glutamate transporter GLAST(EAAT1)deficient mice for phenotypes relevant to the negative and executive/cognitive symptoms of schizophrenia
  • Author(s): Karlsson, RM., Tanaka, K., Saksida,LM., Bussey, TJ., Heilig, M., Holmes, A.
  • Journal Title: Neuropsychopharmacology (in press)
  • Peer Reviewed