Development of consomic mouse model for metabolic syndrome

• Project/Area Number: 19300143
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: University of Tsukuba
• Principal Investigator: SUGIYAMA Fumihiro University of Tsukuba, 大学院・人間総合科学研究科, 准教授 (90226481)
• Co-Investigator(Kenkyū-buntansha): KUNITA Satoshi 筑波大学, 大学院・人間総合科学研究科, 講師 (10195472) ; FUKAMIZU Akiyoshi 筑波大学, 大学院・生命環境科学研究科, 教授 (60199172)
• Project Period (FY): 2007 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2007: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: NZOマウス / 量的形質遺伝子座 / 高血圧 / 肥満 / 糖尿病 / メタボリックシンドローム / 疾患モデル / マウス / コンソミック / QTL / メタポリックシンドローム

Research Abstract

The development of metabolic syndrome is found in NZO/HILtJ mice, but not in C3H/HeJ mice. We identified quantitative trait loci associated with obesity, abnormal glucose metabolism and hypertension. To develop the animal model for understanding the molecular mechanism of metabolic syndrome, we successfully produced three consomic mouse strains, NZO/HILtJ-Chr 7^<C3H/HeJ,>, NZO/HILtJ-Chr 12^<C3H/HeJ> and NZO/HILtJ-Chr 17^<C3H/HeJ>. There were no significant differences in obesity, glucose metabolism and blood pressure between the consomic mice and NZO/HILtJ mice. These results demonstrate that a single major gene associated with metabolic syndrome was not located on the chromosome 7, 12 and 17 of NZO/HILtJ mice. Furthermore, we found Fgf5-deficient natural mutant mice from ICR mouse colony supporting the breeding of NZO/HILtJ mice. The Fgf5-decifient mice might be useful animal model for the basic research of hypertension and metabolic syndrome, because there is strong significant association between hypertension and Fgf5 polymorphism in human.

Research Products

• [Journal Article] Retrotransposon-mediated Fgf5^<go-Utr> mutant mice with long pelage hair. (2011)
  • Author(s): Mizuno S, Iijima S, Okano T, Kajiwara N, Kunita S, Sugiyama F, Yagami K
  • Journal Title: Exp Anim 60 Pages: 161-167
  • NAID: 10029550360
  • Peer Reviewed
• [Journal Article] Retrotransposon-mediated Fgf5^<go-Utr> mutant mice with long pelage hair (2011)
  • Author(s): Mizuno S, et al.
  • Journal Title: Exp Anim Volume: 60 In press
  • Peer Reviewed
• [Journal Article] Genetic analysis of blood pressure in 8 mouse intercross populations. (2009)
  • Author(s): Feng M, Deerhake ME, Keating R, Thaisz J, Xu L, Tsaih SW, Smith R, Ishige T, Sugiyama F, Churchill GA, DiPetrillo K
  • Journal Title: Hypertension 54 Pages: 802-809
  • Peer Reviewed
• [Journal Article] Genetic analysis of blood pressure in 8 mouse intercross populations (2009)
  • Author(s): Feng M, Deerhake ME, Keating R, Thaisz J, Xu L, Tsaih SW, Smith R, Ishige T, Sugiyama F, Churchill GA, DiPetrillo K.
  • Journal Title: Hypertension 54 Pages: 802-809
  • Peer Reviewed
• [Journal Article] Genetic analysis of blood pressure in 8 mouse intercross populations. (2009)
  • Author(s): Feng M, Deerhake ME, Keating R, Thaisz J, Xu L, Tsaih SW, Smith R, Ishige T, Sugiyama F, Churchill GA, DiPetrillo K.
  • Journal Title: Hypertension 54 Pages: 802-809
  • Peer Reviewed
• [Journal Article] Angiotensin type 1 receptor blockade prevents cardiac remodeling in mice with pregnancy-associated hypertension. (2008)
  • Author(s): Sakairi A, Ishida J, Honjo K, Inaba S, Nakamura S, Sugiyama F, Yagami K, Fukamizu A.
  • Journal Title: Hypertens Res 31 Pages: 216-275
  • NAID: 10024828642
  • Peer Reviewed
• [Journal Article] Deterioration of atherosclerosis in mice lacking angiotensin II type 1A receptor in bone marrow-derived cells. (2008)
  • Author(s): Kato H, Ishida J, Nagano K, Honjo K, Sugaya T, Takeda N, Sugiyama F, Yagami K, Fujita T, Nangaku M, Fukamizu A
  • Journal Title: Lab Invest. 88 Pages: 731-739
  • Peer Reviewed
• [Journal Article] Deterioration of atherosclerosis in mice lacking angiotensin II type 1A receptor in bone marrow-derived cells. (2008)
  • Author(s): Kato H, Ishida J, Nagano K, Honjo K, Sugaya T, Takeda N, Sugiyama F, Yagami K, Fujita T, Nangaku M, Fukamizu A.
  • Journal Title: Lab Invest. 88 Pages: 731-739
  • Peer Reviewed
• [Journal Article] Angiotensin type 1 receptor blockade prevents cardiac remodeling in mice with pregnancy-associated hypertension (2008)
  • Author(s): Sakairi A. et al
  • Journal Title: Hypertens Res 31 Pages: 2165-2175
  • Peer Reviewed
• [Journal Article] Deterioration of atherosclerosis in mice lacking angiotensin II type 1A recept or in bone marrow-derived cells. (2008)
  • Author(s): Kato H et al
  • Journal Title: Lab Invest 88 Pages: 731-739
  • Peer Reviewed
• [Journal Article] Quantitative trait loci associated with blood pressure of metabolic syndrome in the progeny of NZO/HILtJxC3H/HeJ intercrosses. (2007)
  • Author(s): Nishihara E, Tsaih SW, Tsukahara C, Langley S, Sheehan S, DiPetrillo K, Kunita S, Yagami K, Churchill GA, Paigen B, Sugiyama F
  • Journal Title: Mamm Genome. 18 Pages: 573-83
  • Peer Reviewed
• [Journal Article] Quantitative trait loci associated with blood pressure of metabolic syndrome in the progeny of NZO/HILtJxC3H/HeJ intercrosses (2007)
  • Author(s): Nishihara E, Tsaih SW, Tsukahara C, Langley S, Sheehan S, DiPetrillo K, Kunita S, Yagami K, Churchill GA, Paigen B, Sugiyama F.
  • Journal Title: Mamm Genome. 18 Pages: 573-83
  • Peer Reviewed
• [Journal Article] Quantitative trait loci associated with blood pressure of metabolic syndrome in the progeny of NZO/HILtJxC3H/HeJ intercrosses. (2007)
  • Author(s): Nishihara E., et. al.
  • Journal Title: Mammalian Genome 18 Pages: 573-583
  • Peer Reviewed
• [Presentation] メタボリックシンドロームを発症するNZOマウスにおける制限給餌の効果 (2009)
  • Author(s): 石毛太一郎、西野入燈、國田智、杉山文博、八神健一
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 埼玉
  • Year and Date: 2009-05-15
• [Presentation] メタボリックシンドロームを発症するNZOマウスにおける制限給餌の効果 (2009)
  • Author(s): 石毛太一郎, 西野入燈, 國田智, 杉山文博, 八神健一
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮ソニックシティ (埼玉県)
  • Year and Date: 2009-05-15
• [Presentation] メタボリックシンドロームを発症するNZOマウスにおける制限給餌の効果 (2009)
  • Author(s): 石毛太一郎、西野入燈、國田智、杉山文博、八神健一
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮ソニックホシティ
  • Year and Date: 2009-05-15
• [Presentation] (NZOxC3H)マウスにおける血圧量的遺伝子座解析 (2007)
  • Author(s): 西原絵里、Tsaih SW、塚原千恵子、DiPetrillo K、Churchill GA、國田智、杉山文博、八神健一
  • Organizer: 第54回日本実験動物学会
  • Place of Presentation: 東京
  • Year and Date: 2007-05-24
• [Presentation] (NZOxC3H) マウスにおける血圧量的遺伝子座解析 (2007)
  • Author(s): 西原絵里, Tsaih SW, 塚原千恵子, DiPetrillo K, Churchi GA, 國田智, 杉山文博, 八神健一
  • Organizer: 第54回日本実験動物学会
  • Place of Presentation: タワーホール船堀 (東京都)
  • Year and Date: 2007-05-24
• [Presentation] (NZOxC#H)F2マウスにおける血圧量的形質遺伝子座解析 (2007)
  • Author(s): 西原絵里, ら
  • Organizer: 日本実験動物学会
  • Place of Presentation: 江戸川区民ホール
  • Year and Date: 2007-05-23