| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2007: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Research Abstract |
For the cryopreservation of fish oocytes, immature oocytes with smaller size and high membrane permeability are suitable. First, we developed a reliable method for in vitro maturation of immature zebrafish oocytes at middle stage III. Then, we showed that exogenous expression of water/cryoprotectant channel (aquaporin 3) in middle stage III oocytes increases the permeability to water and cryoprotectants without decreasing the rates of maturation and development till hatching. However, we did not succeed in the vitrification of oocytes..
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