Cryopreservation of laboratory fish oocytes
| Project/Area Number |
19300147
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kochi University |
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Principal Investigator |
KASAI Magosaburo Kochi University, 教育研究部自然科学系, 教授 (60152617)
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| Co-Investigator(Kenkyū-buntansha) |
EDASHIGE Keisuke 高知大学, 教育研究部自然科学系, 教授 (30175228)
KOSHIMOTO Chihiro 宮崎大学, フロンティア科学実験総合センター, 教授 (70295210)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2007: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 凍結保存 / 魚類卵子 / 水チャンネル / ゼブラフィッシュ / 細胞膜透過性 / 魚類 / 卵子 / 水透過性 / 耐凍剤透過性 / 膜透過性 / 尿素輸送体 |
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| Research Abstract |
For the cryopreservation of fish oocytes, immature oocytes with smaller size and high membrane permeability are suitable. First, we developed a reliable method for in vitro maturation of immature zebrafish oocytes at middle stage III. Then, we showed that exogenous expression of water/cryoprotectant channel (aquaporin 3) in middle stage III oocytes increases the permeability to water and cryoprotectants without decreasing the rates of maturation and development till hatching. However, we did not succeed in the vitrification of oocytes..
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Equilibrium vitrification of mouse embryos2010
Author(s)
Bo Jin, Keiji Mochida, Atsuo Ogura, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keisuke Edashige, Magosaburo Kasai
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Journal Title
Biology of Reproduction 82(2)
Pages: 444-450
Related Report
Peer Reviewed
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[Journal Article]2010
Author(s)
Magosaburo Kasai
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Journal Title
Movement of water and cryoprotectants in mouse oocytes and embryos at different stages : relevance to cryopreservation. In : Chian R, Quinn P(eds.), Fertility Cryopreservation(Cambridge University Press, Cambridge, U.K.)
Pages: 16-23
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[Journal Article] The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae2007
Author(s)
Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M. Valdez Jr., Takao Hara, Bo Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magosaburo Kasai
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Journal Title
Biology of Reproduction 77(2)
Pages: 365-375
Related Report
Peer Reviewed
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[Journal Article] The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae2007
Author(s)
Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M.Valdez Jr., Takao Hara, Bo Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magsaburo Kasai.
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Journal Title
Biology of Reproduction 77
Pages: 365-375
Related Report
Peer Reviewed
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[Presentation] Equilibrium vitrification of mouse embryos2009
Author(s)
Bo Jin, Eri Hotta, Yukiko Kobayashi, Kaori Ito, Go Egawa, Shinsuke Seki, Hiroshi Honda, Keiji Mochida, Atsuo Ogura, Keisuke Edashige, Magosaburo Kasai
Organizer
The 46th Annual Meeting of the Society for Cryobiology
Place of Presentation
Sapporo
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[Presentation] Permeability to water and cryoprotectants of zebrafish (Danio Rerio) oocytes at mid stage III.2008
Author(s)
Delgado M. Valdez, Jr., Ryoma Tsuchiya, Shinsuke Seki, Naoya Saida, Bo Jin, Masahiro Yoshimura, Chihiro Koshimoto, Magosaburo Kasai, Keisuke Edashige
Organizer
The 45th Annual Meeting of the Society for Cryobiology
Place of Presentation
The Omni Charlotte Hotel, Charlotte, NC, USA
Related Report
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[Presentation] The pathway for the movement of water and cryoprotectants in bovine oocytes and embryos.2008
Author(s)
Keisuke Edashige, Takao Hara, Yasunori Kawai, Masahiro Yoshimura, Bo Jin, Delgado M. Valdez, Jr., Magosaburo Kasai
Organizer
The 45th Annual Meeting of the Society for Cryobiology
Place of Presentation
The Omni Charlotte Hotel, Charlotte, NC, USA
Related Report
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[Presentation] ウシ桑実胚の水透過と耐凍剤透過におけるaquaporin 3の役割2008
Author(s)
河合 泰典, 原 隆夫, 松尾完, Valdez M. Delgado Jr., 金 波, 好村 正博, 東山 龍一, 市川 恭子, 恒石 望太郎, 枝重 圭祐, 葛西 孫三郎
Organizer
第101回日本繁殖生物学会
Place of Presentation
福岡県福岡市九州大学医学部
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[Presentation] Short-term storage of vitrified two-cell mouse embryos at-80℃ for handy transportation with dry ice.2008
Author(s)
Bo Jin, Eri Hotta, Yukiko Kobayashi, Shinsuke Seki, Masahiro Yoshimura, Hiroshi Honda, Keiji, Mochida, Atsuo Ogura, Magosaburo Kasai, Keisuke Edashige
Organizer
第101回日本繁殖生物学会
Place of Presentation
福岡県福岡市九州大学医学部
Related Report
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[Presentation] Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio).2008
Author(s)
Shinsuke Seki, Bo Jin, Tsuchiya Ryouma, Toshimitsu Kouya, Takao Hara, Delgado M. Valdez Jr., Magosaburo Kasai, Keisuke Edashige
Organizer
The 5th Annual Conference of the Asia Reproductive Biotechnology Society
Place of Presentation
Horizon Hotel, Kunming, Yunnan Province, China
Related Report
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[Presentation] マウス精子の凍結保存による傷害2007
Author(s)
金 波, 山崎 千寛, 山田 奈央子, 関 信輔, Valdez M.Delgado Jr., 葛西 孫三郎, 枝重 圭祐
Organizer
第100回日本繁殖生物学会大会
Place of Presentation
東京
Year and Date
2007-10-20
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[Presentation] The role of aquaporin-3 in the movement of water and cryoprotectants in mouse morulae2007
Author(s)
Keisuke Edashige, Satoshi Ohta, Mitsunobu Tanaka, Tatsunaga Kuwano, Delgado M. Valdez Jr., Takao Hara, Bo. Jin, Sei-ichi Takahashi, Shinsuke Seki, Chihiro Koshimoto, Magosaburo Kasai
Organizer
The 5th International Conference of Aquaporin
Place of Presentation
Nara
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