Establishment of ES cell lines derived from MSM/Ms and JF1/Ms lines and production of genetically engineered mice
| Project/Area Number |
19300149
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kumamoto University |
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Principal Investigator |
ARAKI Kimi Kumamoto University, 発生医学研究センター, 准教授 (90211705)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKI Masatake 熊本大学, 生命資源研究・支援センター, 准教授 (80271609)
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| Project Period (FY) |
2007 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2008: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2007: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | ES 細胞 / 野生マウス系統 / キメラマウス / 生殖系列キメラ / 遺伝子トラップ / 遺伝子導入 / ES細胞 / 遺伝子操作 |
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| Research Abstract |
MSM及びJF1は、日本固有のモロシヌス亜種から樹立されたマウス系統で、西ヨーロッパ産のマウスから派生した実験用マウスの系統とは、遺伝的にも表現型的にも大きく異なる。我々は、これらの系統で遺伝子導入や遺伝子破壊を可能にするためES細胞を樹立、それらのES細胞からは、生殖系列キメラを作製することができた。さらに、実際に遺伝子トラップによる変異マウス作製およびがん遺伝子のトランスジェニックマウス作製を行った。
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Report
(3 results)
Research Products
(43 results)
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[Journal Article] Establishment of germline-competentembryonic stem cell lines from the MSM/Ms strain2009
Author(s)
Araki, K., Takeda, N., Yoshiki, A., Obata, Y., Nakagata, N., Shiroishi, T., Moriwaki, K. and Yamamura, K.
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Journal Title
Mamm Genome 20
Pages: 14-20
Related Report
Peer Reviewed
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[Journal Article] Inconsistency between hepatic expression and serum concentration of transthyretin in mice humanized at the transthyretin locus2008
Author(s)
Zhao, G., Li, Z., Araki, K., Haruna, K., Yamaguchi, K., Araki, M., Takeya, M., Ando, Y. and Yamamura, K.
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Journal Title
Genes to Cells 13
Pages: 1257-1268
Related Report
Peer Reviewed
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[Journal Article] Expression Pattern of the Serine Protease Inhibitor Kazal Type 3 (Spink3) Gene During the Mouse Embryonic Development2008
Author(s)
Wang, J., Ohmuraya, M., Hirota, M., Baba, H., Araki, K. and Yamamura, K.
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Journal Title
Histochemistry and Cell Biology 130
Pages: 387-397
Related Report
Peer Reviewed
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[Journal Article] Early pre-implantation lethality in mice carrying truncated mutation in the RNA polymerase 1-2 gene2008
Author(s)
Chen, H., Li, Z., Haruna, K., Li, Z., Li. Z., Semba, K., Araki, M., Yamamura, K. and Araki, K.
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Journal Title
Biochem. Biophys. Res. Commun. 365
Pages: 636-642
Related Report
Peer Reviewed
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