| Project/Area Number |
19300150
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
HIRABAYASHI Masumi National Institute for Physiological Sciences, 行動・代謝分子解析センター, 准教授 (20353435)
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| Co-Investigator(Kenkyū-buntansha) |
SHINOHARA Takashi 京都大学, 大学院・医学研究科, 教授 (30322770)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2008: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2007: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | ラット / 蛍光タンパク質遺伝子 / 精子幹細胞 / 人為的活性化 / 円形精子細胞 / 胚性幹細胞 / 精細管移植 / キメラ / トランスジェニック / FGFレセプターインヒビター / MEK活性化インヒビター / GSK3インヒビター / LIF / レンチウイルス / レトロウイルス / 異種間移植 / 精子 / 第二減数分裂後期 / イオノマイシン / 体内受精 / DNAメチル化 |
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| Research Abstract |
The ultimate aim of this study is to establish reproductive technology for production of knock-out rats, by improving microinsemination techniques and producing rat offspring via transfected germ stem cells (GSCs) and embryonic stem cells (ESCs). As the primary result obtained, transgenic rats expressing GFP gene were successfully produced, when the established GSCs were subjected to lentiviral transfection and round spermatid injection. Transgenic rats expressing Kusabira-Orange gene were also produced via blastocyst injection of ESCs established and transfected by electroporation. It was shown for the first time that the GSCs and ESCs can contribute to the production of transgenic offspring in the rats.
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