Coordination and dissociation of nonlinear oscillations in cardiomyocytes : dysfunction of holonic oscillators leading to arrhythmias
| Project/Area Number |
19300153
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
KAWAHARA Koichi Hokkaido University, 大学院・情報科学研究科, 教授 (20125397)
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| Co-Investigator(Kenkyū-buntansha) |
OKU Yoshitaka 兵庫医科大学, 医学部, 教授 (20252512)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
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| Keywords | 心筋細胞 / 細胞内Ca^<2+>振動 / ATP-purinoceptor系 / 細胞外ATP振動 / 細胞内Mg^<2+>振動 / 非線形振動子 / 負帰還制御系 / ATP-purinocetor系 |
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| Research Abstract |
We have recently found that the extracellular ATP-purinoceptor signaling is crucially involved in the intercellular synchronization of intracellular Ca^<2+> oscillations among cardiomyocytes. In this study, I aim at elucidating whether and how the concentrations of extracellular ATP and intracellular Mg^<2+> oscillate in association with rhythmic contraction of cultured cardiomyocytes from neonatal rats. The simultaneous measurement of the fluo-4 fluorescence and the luciferin/luciferase chemical luminescence showed that the concentration of extracellular ATP oscillated synchronously with the concentration of intracellular Ca^<2+> in cardiomyocytes, supporting our previous inference that the extracellular ATP-purinoceptor signaling is responsible for the intercellular synchronization of intracellular Ca^<2+> oscillations. In addition, the concentration of intracellular Mg^<2+> also oscillated in association with rhythmic contraction and intracellular Ca^<2+> oscillations, suggesting that the concentration of intracellular ATP varied depending on the rhythmic contraction of cardiomyocytes. All these findings have led to an idea that the coordination of multiple nonlinear oscillations in cardiomyocytes such as rhythmic variations in the concentrations of intracellular Ca^<2+> Mg^<2+> and ATP seems critical to the normal function of the heart.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Power-Law stress and creep relaxations of single cells measured by colloidal probe atomic force microscopy2009
Author(s)
Hiratsuka, S., Mizutani, Y., Toda, A., Fukushima, N., Kawahara, K., Tokumoto, H., Okajima. T.
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Journal Title
Japanese Journal of Applied Physics 48
NAID
Related Report
Peer Reviewed
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[Journal Article] The number distribution of complex shear modulus of single cells measured by atomic force microscopy2009
Author(s)
Hiratsuka, S., Mizutani, Y., Tsuchiya, M., Kawahara, K., Tokumoto, H., Okajima, T.
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Journal Title
Ultramicroscopy 109
Pages: 937-941
Related Report
Peer Reviewed
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[Journal Article] Elasticity of living cells on a microarray during the early stages of adhesion measured by atomic force microscopy2008
Author(s)
Mizutani, Y., Tsuchia, M., Hiratsuka, S., Kawahara, K., Tokumoto, H., Okajima, T.
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Journal Title
Japanese Journal of Applied Physics 47(7)
Pages: 6177-6180
Related Report
Peer Reviewed
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[Presentation] Elasticity of Cardiac Cells on a Microarray under Oxidative Stress Measured by Atomic Force Microscopy. Y. Mizutani, M. Tsuchiya, S. Hiratsuka, K. Kawahara, H. Tokumoto, and T. Okajima2008
Author(s)
Mizutani, Y., Tsuchiya, M., Hiratsuka, S., Kawahara, K., Tokumoto, H., Okajima, T.
Organizer
International Scanning Probe Microscopy Conference Seattle '08, Seattle, WA, U.S.A.
Place of Presentation
International Scanning Probe Microscopy Conference Seattle Meeting program
Related Report
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[Presentation] Force measurements of microarray living cells2007
Author(s)
Mizutani, Y., Tsuchiya, M., Hiratsuka, S., Kawahara, K., Tokumoto, H., Okajima, T.
Organizer
Proceedings of the 15^<th> International Colloquium on Scanning Probe Microscopy (ICSPM15)
Place of Presentation
Atagawa, Japan
Related Report
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