| Project/Area Number |
19300158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
TOOYAMA Ikuo Shiga University of Medical Science, 分子神経科学研究センター, 教授 (20207533)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Hiroyasu 滋賀医科大学, 分子神経科学研究センター, 特任教授 (90102912)
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| Co-Investigator(Renkei-kenkyūsha) |
INUBUSHI Toshiro 滋賀医科大学, MR医学総合研究センター, 教授 (20213142)
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| Project Period (FY) |
2007 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2009: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2007: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | パーキンソン病 / 核磁気共鳴 / 診断薬 / フッ素MR画像法 / 超常磁性酸化鉄 / 核磁気共鳴画像 |
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| Research Abstract |
We examined the feasibility of high filed MRI, particularly ^<19>F-MRI, for the diagnosis of Parkinson disease using PC12 cells and 6OH-dopamine treated rats. We measured ^<19>F-NMR signals in the homogenates of rat brain and several agents such as 6-^<19>F-fluoro-L-DOPA (^<19>F-DOPA) and chemicals that are involved in the activity of dopamine neurons. Among them, ^<19>F-DOPA showed the best signal to nose ratio. When ^<19>F-DOPA was added into culture medium of PC12 cells, MR images of ^<19>F-DOPA in about 50 mg of PC12 cells were successfully detected. When we measured the brain from 6-OH-dopamine treated rats that received intra-peritoneal injection of ^<19>F-DOPA, MR images of ^<19>F-DOPA in the striatum were successfully detected by 10 hours measurements of 7-tesla MRI. This study gives us the fundamental data for the development of diagnostic methods for Parkinson disease using high fieled MRI.
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