| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2007: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Research Abstract |
Techniques of chemical release control for differentiation induction of neural stem cells were developed. First, four nanohole arrays and microvalves were fabricated at the bottom of a cell-culturing chamber, and chemical release from the nanoholes was controlled by switching open/close of the microvalves. Next, a sheathflow was generated in a microchannel under a chamber, and chemical release from microholes was controlled by regulating flow rates of three fluids which form the sheathflow. Moreover, in order to permit multiple chemicals to be released on a single device, 64 chambers were arranged in an 8 x 8 matrix, and each of 8 microchannels was located directly under a row of chambers.
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